BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0131
(511 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g51730.1 68414.m05829 RWD domain-containing protein contains ... 27 7.3
At5g59900.1 68418.m07512 pentatricopeptide (PPR) repeat-containi... 27 9.7
At4g30120.1 68417.m04282 ATPase E1-E2 type family protein / heav... 27 9.7
At4g19500.1 68417.m02868 disease resistance protein (TIR-NBS-LRR... 27 9.7
At2g04305.1 68415.m00423 magnesium transporter CorA-like protein... 27 9.7
>At1g51730.1 68414.m05829 RWD domain-containing protein contains
Pfam profile PF05773: RWD domain; similar to GCN2
eIF2alpha kinase (GI:6066585) [Mus musculus]; similar to
GCN2beta (GI:10764163) [Mus musculus]; similar to RING
finger protein 25 (RING finger protein AO7)
(Swiss-Prot:Q9QZR0) [Mus musculus]; similar to RING
finger protein 25 (Swiss-Prot:Q96BH1) [Homo sapiens]
Length = 252
Score = 27.1 bits (57), Expect = 7.3
Identities = 15/54 (27%), Positives = 22/54 (40%)
Frame = +3
Query: 117 RNKYKRSRQWKLFGYNIDGVIYTSDSDFEHKRGSFTFKDELLEDHEKILIRKNL 278
+ K RQW G V+ + D E F+DE ED E+ ++ L
Sbjct: 186 KEKKLTGRQWFESGRGRGTVVIADEEDEEEDEEDIDFEDEDFEDDEEDMLEHYL 239
>At5g59900.1 68418.m07512 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 907
Score = 26.6 bits (56), Expect = 9.7
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +3
Query: 156 GYNIDGVIYTSDSDFEHKRGSFTFKDELLEDHEKILIRKNLVPNHINY 299
G D VIYTS D + K G F + + ++I + VPN + Y
Sbjct: 677 GLKPDDVIYTSMIDAKSKTGDFKEAFGIWD----LMINEGCVPNEVTY 720
>At4g30120.1 68417.m04282 ATPase E1-E2 type family protein /
heavy-metal-associated domain-containing protein similar
to cadmium efflux ATPase [Streptococcus thermophilus]
GI:22416341; contains Pfam profile PF00122: E1-E2 ATPase
Length = 542
Score = 26.6 bits (56), Expect = 9.7
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -3
Query: 506 SC*ATYITLTGQQFPLQNSSFLTVIASASDTTSPLQLKLT 387
SC TLTG+ FP+ TV+A+ + +++K T
Sbjct: 240 SCDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTT 279
>At4g19500.1 68417.m02868 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein. A false
intron was added between exons 2 and 3 to circumvent a
frameshift caused by a sequencing error, as per Blake
Meyers (bcmeyers@vegmail.ucdavis.edu)
Length = 1308
Score = 26.6 bits (56), Expect = 9.7
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = -3
Query: 335 PCPRNRYYCTKEIVDVVRN 279
PC R+R + +K+IVDV+ N
Sbjct: 1045 PCERSRLWDSKDIVDVLTN 1063
>At2g04305.1 68415.m00423 magnesium transporter CorA-like
protein-related
Length = 434
Score = 26.6 bits (56), Expect = 9.7
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +3
Query: 195 DFEHKRGSFTFKDELLEDHEKILIRKNL-----VPNHINYLFRTIIPIPRTRPESYSDQI 359
+ E RG F K ++L+D + NL V H +F + T+P ++ I
Sbjct: 228 ELEMDRGGFAMKKKMLDDRRFPKLHLNLQRLLQVIAHGEQVFPRVKEKCSTKPWFLAEDI 287
Query: 360 HS-SEIAQRISELK 398
HS E+ R+ LK
Sbjct: 288 HSLEELIGRLRRLK 301
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,725,281
Number of Sequences: 28952
Number of extensions: 181446
Number of successful extensions: 418
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 413
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 418
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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