BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0058 (639 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g23690.1 68418.m02777 polynucleotide adenylyltransferase fami... 40 0.001 At2g17580.1 68415.m02034 polynucleotide adenylyltransferase fami... 40 0.001 At1g28090.2 68414.m03439 polynucleotide adenylyltransferase fami... 40 0.001 At1g28090.1 68414.m03438 polynucleotide adenylyltransferase fami... 40 0.001 At1g22660.2 68414.m02832 tRNA-nucleotidyltransferase, putative /... 39 0.002 At1g22660.1 68414.m02831 tRNA-nucleotidyltransferase, putative /... 39 0.002 At3g48830.1 68416.m05333 polynucleotide adenylyltransferase fami... 38 0.004 At2g20030.1 68415.m02341 zinc finger (C3HC4-type RING finger) fa... 30 1.5 At5g37420.1 68418.m04502 hypothetical protein contains Pfam PF04... 29 2.6 At5g57480.1 68418.m07183 AAA-type ATPase family protein contains... 29 3.4 At1g76280.1 68414.m08858 pentatricopeptide (PPR) repeat-containi... 27 7.9 >At5g23690.1 68418.m02777 polynucleotide adenylyltransferase family protein low similarity to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 527 Score = 39.9 bits (89), Expect = 0.001 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = +1 Query: 304 HEIRIAGGAVRDLLMGLTPKDLDFATTATPEEMKEMFTAEEV 429 H++ + GG VRDL++ TPKD D T+A E+ F E+ Sbjct: 95 HDVYLVGGCVRDLILKRTPKDFDILTSAELREVVRTFPRCEI 136 >At2g17580.1 68415.m02034 polynucleotide adenylyltransferase family protein similar to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 757 Score = 39.9 bits (89), Expect = 0.001 Identities = 17/60 (28%), Positives = 34/60 (56%) Frame = +1 Query: 250 SIFTEEVIDLKKIFDKYKHEIRIAGGAVRDLLMGLTPKDLDFATTATPEEMKEMFTAEEV 429 S+ E +++ ++ + + + GG VRDL++ PKD D TTA ++++ +F +V Sbjct: 72 SMIPESSMNVLRLLRRQGFDAYLVGGCVRDLILNRVPKDYDVITTADLKQIRRLFHRAQV 131 >At1g28090.2 68414.m03439 polynucleotide adenylyltransferase family protein low similarity to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 505 Score = 39.9 bits (89), Expect = 0.001 Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 3/66 (4%) Frame = +1 Query: 226 KVDSPEF---KSIFTEEVIDLKKIFDKYKHEIRIAGGAVRDLLMGLTPKDLDFATTATPE 396 K+D+ EF +S+ + + K ++ + GG VRDL++ PKD D TTA + Sbjct: 48 KLDANEFGIQRSMIPDSTRMVLNKLKKKGFQVYLVGGCVRDLILDRIPKDFDVITTAELK 107 Query: 397 EMKEMF 414 E++++F Sbjct: 108 EVRKVF 113 >At1g28090.1 68414.m03438 polynucleotide adenylyltransferase family protein low similarity to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 541 Score = 39.9 bits (89), Expect = 0.001 Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 3/66 (4%) Frame = +1 Query: 226 KVDSPEF---KSIFTEEVIDLKKIFDKYKHEIRIAGGAVRDLLMGLTPKDLDFATTATPE 396 K+D+ EF +S+ + + K ++ + GG VRDL++ PKD D TTA + Sbjct: 84 KLDANEFGIQRSMIPDSTRMVLNKLKKKGFQVYLVGGCVRDLILDRIPKDFDVITTAELK 143 Query: 397 EMKEMF 414 E++++F Sbjct: 144 EVRKVF 149 >At1g22660.2 68414.m02832 tRNA-nucleotidyltransferase, putative / tRNA adenylyltransferase, putative similar to tRNA nucleotidyltransferase [Lupinus albus] GI:1139585; contains Pfam profile PF01743: polyA polymerase family protein Length = 603 Score = 39.1 bits (87), Expect = 0.002 Identities = 38/116 (32%), Positives = 63/116 (54%), Gaps = 13/116 (11%) Frame = +1 Query: 307 EIRIAGGAVRDLLMGLTPKDLDFAT-----TATPEEMKEMFTA--EEVR---MINVNGER 456 ++R+AGG VRD L+G D+D A + ++ KE ++ EEV+ +I N ++ Sbjct: 109 QLRVAGGWVRDKLLGKESDDIDIAIDNMSGSEFLDKFKEYLSSRDEEVQGDTVIERNPDQ 168 Query: 457 HGTI-TARINDKENF-EVTTLRIDMVTD-GRFADVQFTKDWKLDANRRDLTINSMF 615 + TA++ + + + LR + T+ R ++F K DA RRDLTINS+F Sbjct: 169 SKHLETAKLRIYDQWIDFVNLRSEEYTENSRIPTMKFGTA-KDDAFRRDLTINSLF 223 >At1g22660.1 68414.m02831 tRNA-nucleotidyltransferase, putative / tRNA adenylyltransferase, putative similar to tRNA nucleotidyltransferase [Lupinus albus] GI:1139585; contains Pfam profile PF01743: polyA polymerase family protein Length = 605 Score = 39.1 bits (87), Expect = 0.002 Identities = 38/116 (32%), Positives = 63/116 (54%), Gaps = 13/116 (11%) Frame = +1 Query: 307 EIRIAGGAVRDLLMGLTPKDLDFAT-----TATPEEMKEMFTA--EEVR---MINVNGER 456 ++R+AGG VRD L+G D+D A + ++ KE ++ EEV+ +I N ++ Sbjct: 109 QLRVAGGWVRDKLLGKESDDIDIAIDNMSGSEFLDKFKEYLSSRDEEVQGDTVIERNPDQ 168 Query: 457 HGTI-TARINDKENF-EVTTLRIDMVTD-GRFADVQFTKDWKLDANRRDLTINSMF 615 + TA++ + + + LR + T+ R ++F K DA RRDLTINS+F Sbjct: 169 SKHLETAKLRIYDQWIDFVNLRSEEYTENSRIPTMKFGTA-KDDAFRRDLTINSLF 223 >At3g48830.1 68416.m05333 polynucleotide adenylyltransferase family protein / RNA recognition motif (RRM)-containing protein similar to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profiles PF01743: polyA polymerase family protein, PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Length = 881 Score = 38.3 bits (85), Expect = 0.004 Identities = 16/42 (38%), Positives = 26/42 (61%) Frame = +1 Query: 304 HEIRIAGGAVRDLLMGLTPKDLDFATTATPEEMKEMFTAEEV 429 +++ + GG VRDL++ TPKD D T+A E+ F+ E+ Sbjct: 92 YDVYLVGGCVRDLILKRTPKDFDILTSAELREVVRSFSRCEI 133 >At2g20030.1 68415.m02341 zinc finger (C3HC4-type RING finger) family protein similar to RING-H2 zinc finger protein ATL6 [Arabidopsis thaliana] GI:4928403; contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger) Length = 390 Score = 29.9 bits (64), Expect = 1.5 Identities = 23/85 (27%), Positives = 40/85 (47%) Frame = +1 Query: 265 EVIDLKKIFDKYKHEIRIAGGAVRDLLMGLTPKDLDFATTATPEEMKEMFTAEEVRMINV 444 E ID KK+ K+ H I ++ ++ +T DL F T+ EM +++ R +V Sbjct: 236 ENIDEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTS----EMLNSVSSD--RFSSV 289 Query: 445 NGERHGTITARINDKENFEVTTLRI 519 + G + DKE+ E+ + I Sbjct: 290 DRVHRG----NLRDKEDMEMKRMLI 310 >At5g37420.1 68418.m04502 hypothetical protein contains Pfam PF04510 : Family of unknown function (DUF577)); common family comprised of At5g37410, At5g37400, At5g37920, At5g37460, At5g37650, At5g37470, At5g37420, At5g37430 Length = 579 Score = 29.1 bits (62), Expect = 2.6 Identities = 17/58 (29%), Positives = 32/58 (55%) Frame = -2 Query: 623 NPKNIEFIVRSLLLASSFQSLVN*TSAKRPSVTISILKVVTSKFSLSLIRAVIVPCLS 450 NP ++ + + +SS S++ S + S T++ +K S SL I+ V++PCL+ Sbjct: 54 NPSSLTLKLLMVYQSSSSNSVLRFRSIYQLSQTLTDVKFKPSLDSLFEIKGVLIPCLT 111 >At5g57480.1 68418.m07183 AAA-type ATPase family protein contains Pfam profile: PF00004 ATPase family Length = 520 Score = 28.7 bits (61), Expect = 3.4 Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 4/62 (6%) Frame = -2 Query: 269 TSSVNILLNSGESTLITIGFSRHFKFKSI----FDRNLLRLGSVTLHVPLSWKNCPTPKI 102 T +++ LLN + G R F F + D LLR G + +H+ +S+ N P+ KI Sbjct: 344 TITLSGLLNFTDGLWSCCGSERIFVFTTNHIEKLDPALLRSGRMDMHIYMSFCNFPSLKI 403 Query: 101 LL 96 LL Sbjct: 404 LL 405 >At1g76280.1 68414.m08858 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 773 Score = 27.5 bits (58), Expect = 7.9 Identities = 22/78 (28%), Positives = 32/78 (41%) Frame = -2 Query: 455 LSPLTFIILTSSAVNISFISSGVAVVAKSRSFGVSPIKRSLTAPPAILISCLYLSKIFFR 276 LSPL FI+ +SS+ ++S G + G S + T I C SKI Sbjct: 7 LSPLRFIVSSSSSKLTPYVSRGRGLSGIDNGAGCSCSRSVTTMIGNEFIRCQDESKILQL 66 Query: 275 SITSSVNILLNSGESTLI 222 I ++ G S L+ Sbjct: 67 QIVDALRSGERQGASALL 84 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,789,737 Number of Sequences: 28952 Number of extensions: 252547 Number of successful extensions: 706 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 690 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 704 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1314848736 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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