Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0028
(447 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 31 0.11
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 30 0.14
SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.25
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 28 0.57
SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 28 0.75
SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 27 0.99
SPBC2A9.02 |||NAD dependent epimerase/dehydratase family protein... 26 3.0
SPCC338.15 |||dolichyl-di-phosphooligosaccharide-protein glycotr... 26 3.0
SPCC18B5.06 |erf1|sup45|translation release factor eRF1|Schizosa... 25 4.0
SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr 1|||M... 25 7.0
SPAC5D6.05 |sep11|pmc6, med18|mediator complex subunit Pmc6 |Sch... 24 9.3
SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|c... 24 9.3
SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizos... 24 9.3
SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom... 24 9.3
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 30.7 bits (66), Expect = 0.11
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = -1
Query: 279 VSNQNVNAYYARTRIRKFLNVRLSKESGRSCNKNGFTIVKFPHGLIMFIQSSI 121
V+N+N Y +LN RLS++ NGFT + PH L MF +S I
Sbjct: 857 VTNEN-RLQYIHLVSNYYLNARLSRQC--RAFTNGFTQIIDPHWLAMFHESEI 906
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 30.3 bits (65), Expect = 0.14
Identities = 14/48 (29%), Positives = 27/48 (56%)
Frame = +1
Query: 166 DGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERM 309
+G+ +TGATG+ G+ ++E Y +S+ L+R+ +ER+
Sbjct: 643 NGQYFLLTGATGYFGRRFLE---YLVKLNISVVCLVRESSDEAAKERL 687
>SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 600
Score = 29.5 bits (63), Expect = 0.25
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +1
Query: 271 IRDKKGSNTEERMRKYLDQPIFSRIKYEHPEYFKKII 381
+ K SN E+R+ KY+ R +HP++ K++
Sbjct: 393 VHQKLNSNLEKRVEKYIKHSCSQRNVADHPDFALKVV 429
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 28.3 bits (60), Expect = 0.57
Identities = 23/91 (25%), Positives = 43/91 (47%)
Frame = +1
Query: 172 KSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMRKYLDQPIFSRIKY 351
K+VF+TGA G+LG + L + + L+R S+ E +++ D +
Sbjct: 1005 KTVFLTGANGYLGVFILRDLMTRSSNL-KVIALVR---ASSEEHGLKRLKDSCTAYGVWD 1060
Query: 352 EHPEYFKKIIPISGDITAPKLGLCDEERNIL 444
E + +KI ++GD+ G+ + + N L
Sbjct: 1061 E--SWAQKISVVNGDLALENWGIEERKWNKL 1089
>SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 259
Score = 27.9 bits (59), Expect = 0.75
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = +1
Query: 166 DGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEE 303
DGK++ ITGA+ +GK+ A+ + + +++ ++ S EE
Sbjct: 5 DGKTILITGASSGIGKS----TAFEIAKVAKVKLILAARRFSTVEE 46
>SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 0.99
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1
Query: 169 GKSVFITGATGFLGKAYVEKL 231
GK V +TG TGF+G E+L
Sbjct: 3 GKLVLVTGVTGFIGAHVAEQL 23
>SPBC2A9.02 |||NAD dependent epimerase/dehydratase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 295
Score = 25.8 bits (54), Expect = 3.0
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 178 VFITGATGFLGKAYVEKL 231
+F+TGA GF+G V +L
Sbjct: 3 IFVTGAAGFIGSEIVRQL 20
>SPCC338.15 |||dolichyl-di-phosphooligosaccharide-protein
glycotransferase subunit |Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 25.8 bits (54), Expect = 3.0
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = -2
Query: 443 NIFLSSSHNPSLGAVISPEMGMIFLKYSGCSYFI 342
N+ L SS + SLG V SP+ + F++ SG + F+
Sbjct: 71 NLILLSSQSKSLGPVFSPKSLLEFVQ-SGGNLFV 103
>SPCC18B5.06 |erf1|sup45|translation release factor
eRF1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 4.0
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
Frame = +1
Query: 22 NTCLI*TIISLLRKNCDQLQIIFKFRVISKMSHNGTLDEHYQTV-----REF-YDGKSVF 183
N CLI +++LR+ D Q+I + R ++ LD+ Y +V EF +D V
Sbjct: 148 NICLITDYMTILRQRID--QVIPRKRRGDSSAYQKGLDKFYDSVFQSINSEFDFDKLKVV 205
Query: 184 ITGATGFLGK 213
I + GF+ +
Sbjct: 206 ILASPGFVAR 215
>SPAC4G9.15 |||ketoreductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 24.6 bits (51), Expect = 7.0
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +1
Query: 184 ITGATGFLGKAYVEKLAYSCPGIVSI 261
+TGAT +GK Y +LA S +V I
Sbjct: 62 VTGATDGIGKEYATQLAMSGFNVVLI 87
>SPAC5D6.05 |sep11|pmc6, med18|mediator complex subunit Pmc6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 207
Score = 24.2 bits (50), Expect = 9.3
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 178 RIYHRKIPSRSDNVHPKFH 122
RIY IPS+ ++ P FH
Sbjct: 139 RIYQTLIPSQQRSIKPPFH 157
>SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 392
Score = 24.2 bits (50), Expect = 9.3
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +1
Query: 112 MSHNGTLDEHYQTVREFYDGKSVFITG 192
+SH +D YQ VR F D + + + G
Sbjct: 110 LSHVRAVDAIYQVVRAFDDAEIIHVEG 136
>SPBC8D2.01 |gsk31||serine/threonine protein kinase
Gsk31|Schizosaccharomyces pombe|chr 2|||Manual
Length = 381
Score = 24.2 bits (50), Expect = 9.3
Identities = 14/55 (25%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +1
Query: 196 TGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMRKYLDQP-IFSRIKYEH 357
+G G KL PG +++ +++DK+ N E ++ + + P I I + H
Sbjct: 33 SGSFGVVIQAKLV-GTPGFIAVKRVLQDKRYKNRELQIMRAISHPNIIKLIAFFH 86
>SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 397
Score = 24.2 bits (50), Expect = 9.3
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1
Query: 166 DGKSVFITGATGFLGKAYVEKLA 234
+G V +TG + +G+ VEKLA
Sbjct: 74 NGMVVMVTGGSSGIGQVVVEKLA 96
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,948,336
Number of Sequences: 5004
Number of extensions: 40597
Number of successful extensions: 109
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 164204010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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