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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0028
         (447 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g56700.1 68416.m06307 male sterility protein, putative simila...    61   3e-10
At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identica...    61   3e-10
At3g44550.1 68416.m04788 acyl CoA reductase, putative similar to...    56   1e-08
At5g22500.1 68418.m02626 acyl CoA reductase, putative / male-ste...    55   3e-08
At3g44560.1 68416.m04789 acyl CoA reductase, putative similar to...    54   6e-08
At3g44540.1 68416.m04787 acyl CoA reductase, putative similar to...    51   4e-07
At5g22420.1 68418.m02615 acyl CoA reductase, putative similar to...    43   8e-05
At2g28760.2 68415.m03498 NAD-dependent epimerase/dehydratase fam...    33   0.12 
At2g28760.1 68415.m03497 NAD-dependent epimerase/dehydratase fam...    33   0.12 
At1g60110.1 68414.m06772 jacalin lectin family protein similar t...    29   1.4  
At1g08200.1 68414.m00906 expressed protein                             29   1.4  
At5g59450.1 68418.m07451 scarecrow-like transcription factor 11 ...    28   3.3  
At4g38850.1 68417.m05500 auxin-responsive protein / small auxin ...    28   3.3  
At2g34460.1 68415.m04229 flavin reductase-related low similarity...    27   4.4  
At2g21280.1 68415.m02533 expressed protein similar to YfhF (GI:2...    27   4.4  
At5g06060.1 68418.m00671 tropinone reductase, putative / tropine...    27   5.8  
At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S...    27   5.8  
At3g14790.1 68416.m01869 NAD-dependent epimerase/dehydratase fam...    27   7.6  
At2g11623.1 68415.m01250 hypothetical protein                          27   7.6  

>At3g56700.1 68416.m06307 male sterility protein, putative similar
           to SP|Q08891 Male sterility protein 2 {Arabidopsis
           thaliana}; contains Pfam profile PF03015: Male sterility
           protein
          Length = 527

 Score = 61.3 bits (142), Expect = 3e-10
 Identities = 34/101 (33%), Positives = 54/101 (53%), Gaps = 6/101 (5%)
 Frame = +1

Query: 160 FYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERM-RKYLDQPIF 336
           F +GKS  +TGATGFL K  +EKL      I  I++L+R K   +  +R+  + +   +F
Sbjct: 80  FLEGKSYLVTGATGFLAKVLIEKLLRESLEIGKIFLLMRSKDQESANKRLYDEIISSDLF 139

Query: 337 SRIKYEHPEYF-----KKIIPISGDITAPKLGLCDEERNIL 444
             +K  H   +     +K+IP+ GDI    LG+  E  N++
Sbjct: 140 KLLKQMHGSSYEAFMKRKLIPVIGDIEEDNLGIKSEIANMI 180


>At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identical
           to male sterility protein 2 (MS2) SP:Q08891 (Arabidopsis
           thaliana)
          Length = 616

 Score = 61.3 bits (142), Expect = 3e-10
 Identities = 33/93 (35%), Positives = 51/93 (54%), Gaps = 6/93 (6%)
 Frame = +1

Query: 160 FYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIF 336
           F  GK   ITG+TGFL K  +EK+    P +  IY+LI+ K      ER++ + LD  +F
Sbjct: 127 FLQGKKFLITGSTGFLAKVLIEKVLRMAPDVSKIYLLIKAKSKEAAIERLKNEVLDAELF 186

Query: 337 SRIKYEHPEYF-----KKIIPISGDITAPKLGL 420
           + +K  H   +      K+IP++G+I    +GL
Sbjct: 187 NTLKETHGASYMSFMLTKLIPVTGNICDSNIGL 219


>At3g44550.1 68416.m04788 acyl CoA reductase, putative similar to
           acyl CoA reductase [Simmondsia chinensis] GI:5020215;
           contains Pfam profile PF03015: Male sterility protein
          Length = 402

 Score = 55.6 bits (128), Expect = 1e-08
 Identities = 27/97 (27%), Positives = 53/97 (54%), Gaps = 7/97 (7%)
 Frame = +1

Query: 157 EFYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPI 333
           +F   K++ +TGATGFL K +VEK+    P +  +Y+L+R        +R+R +  ++ +
Sbjct: 7   QFLRNKTILVTGATGFLAKVFVEKILRVQPNVKKLYLLVRASDNEAATKRLRTEVFEKEL 66

Query: 334 FSRIKYE------HPEYFKKIIPISGDITAPKLGLCD 426
           F  ++        +   ++K++ + GDI   +LG+ D
Sbjct: 67  FKVLRQNLGDEKLNTLLYEKVVSVPGDIATDQLGIND 103


>At5g22500.1 68418.m02626 acyl CoA reductase, putative /
           male-sterility protein, putative similar to acyl CoA
           reductase [Simmondsia chinensis] GI:5020215; contains
           Pfam profile PF03015: Male sterility protein; identical
           to cDNA male sterility 2-like protein GI:1491614
          Length = 491

 Score = 54.8 bits (126), Expect = 3e-08
 Identities = 32/99 (32%), Positives = 53/99 (53%), Gaps = 7/99 (7%)
 Frame = +1

Query: 157 EFYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPI 333
           +F   K++ ITGA GFL K  VEK+    P +  IY+L+R     +  +R+R + ++  +
Sbjct: 7   QFLGNKTILITGAPGFLAKVLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEIDL 66

Query: 334 FSRIKYEHPE------YFKKIIPISGDITAPKLGLCDEE 432
           F  ++    E        +KI+P+ GDI+   LGL D +
Sbjct: 67  FKVLRNNLGEDNLNALMREKIVPVPGDISIDNLGLKDTD 105


>At3g44560.1 68416.m04789 acyl CoA reductase, putative similar to
           acyl CoA reductase [Simmondsia chinensis] GI:5020215;
           contains Pfam profile PF03015: Male sterility protein
          Length = 449

 Score = 53.6 bits (123), Expect = 6e-08
 Identities = 27/96 (28%), Positives = 52/96 (54%), Gaps = 7/96 (7%)
 Frame = +1

Query: 160 FYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIF 336
           F   K++ +TGATGFL K +VEK+    P +  +Y+++R        +R+R +  ++ +F
Sbjct: 8   FLQNKTILVTGATGFLAKVFVEKILRVQPNVNKLYLVVRASDNEAATKRLRTEAFEKDLF 67

Query: 337 SRIKYE------HPEYFKKIIPISGDITAPKLGLCD 426
             ++        +    +K++P++GDI    LG+ D
Sbjct: 68  KVLRDNLGDEKLNTLLSEKVVPVAGDIAMDHLGMKD 103


>At3g44540.1 68416.m04787 acyl CoA reductase, putative similar to
           acyl CoA reductase [Simmondsia chinensis] GI:5020215;
           contains Pfam profile PF03015: Male sterility protein
          Length = 433

 Score = 50.8 bits (116), Expect = 4e-07
 Identities = 28/95 (29%), Positives = 53/95 (55%), Gaps = 7/95 (7%)
 Frame = +1

Query: 157 EFYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPI 333
           +F   K++ +TG  GFL K +VEK+    P +  +++L+R     +  +R   + L++ +
Sbjct: 7   QFLHDKTILVTGVPGFLAKVFVEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKDL 66

Query: 334 FSRIKY----EHPEYF--KKIIPISGDITAPKLGL 420
           F  +K     E+ + F  +K++PI GDI+   LG+
Sbjct: 67  FRVLKNALGDENLKAFITEKVVPIPGDISVDNLGV 101


>At5g22420.1 68418.m02615 acyl CoA reductase, putative similar to
           acyl CoA reductase [Simmondsia chinensis] GI:5020215;
           contains Pfam profile PF03015: Male sterility protein
          Length = 409

 Score = 43.2 bits (97), Expect = 8e-05
 Identities = 16/50 (32%), Positives = 31/50 (62%)
 Frame = +1

Query: 157 EFYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEER 306
           +F + +++ +TGA+GFL K  VE++    P +  +Y+L+R     + E+R
Sbjct: 7   QFLENRTILVTGASGFLAKVLVERILRLQPNVKRLYLLVRASDKKSAEQR 56


>At2g28760.2 68415.m03498 NAD-dependent epimerase/dehydratase family
           protein similar to UDP-glucuronic acid decarboxylase
           Uxs1p from Filobasidiella neoformans GI:14318327;
           contains Pfam profile PF01370 NAD dependent
           epimerase/dehydratase family
          Length = 343

 Score = 32.7 bits (71), Expect = 0.12
 Identities = 15/68 (22%), Positives = 34/68 (50%)
 Frame = +1

Query: 160 FYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMRKYLDQPIFS 339
           F     + +TG  GF+G   V+KL  +        +++ D   + +++ ++K++  P F 
Sbjct: 27  FQSNMRILVTGGAGFIGSHLVDKLMQN----EKNEVIVADNYFTGSKDNLKKWIGHPRFE 82

Query: 340 RIKYEHPE 363
            I+++  E
Sbjct: 83  LIRHDVTE 90


>At2g28760.1 68415.m03497 NAD-dependent epimerase/dehydratase family
           protein similar to UDP-glucuronic acid decarboxylase
           Uxs1p from Filobasidiella neoformans GI:14318327;
           contains Pfam profile PF01370 NAD dependent
           epimerase/dehydratase family
          Length = 343

 Score = 32.7 bits (71), Expect = 0.12
 Identities = 15/68 (22%), Positives = 34/68 (50%)
 Frame = +1

Query: 160 FYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMRKYLDQPIFS 339
           F     + +TG  GF+G   V+KL  +        +++ D   + +++ ++K++  P F 
Sbjct: 27  FQSNMRILVTGGAGFIGSHLVDKLMQN----EKNEVIVADNYFTGSKDNLKKWIGHPRFE 82

Query: 340 RIKYEHPE 363
            I+++  E
Sbjct: 83  LIRHDVTE 90


>At1g60110.1 68414.m06772 jacalin lectin family protein similar to
           myrosinase-binding protein homolog [Arabidopsis
           thaliana] GI:2997767; contains Pfam profile PF01419
           jacalin-like lectin domain
          Length = 598

 Score = 29.1 bits (62), Expect = 1.4
 Identities = 16/62 (25%), Positives = 25/62 (40%)
 Frame = +1

Query: 100 VISKMSHNGTLDEHYQTVREFYDGKSVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRD 279
           ++     N   DEH ++V  +YD KS  I G          E + Y+  G     + +  
Sbjct: 66  ILQTFDINHKNDEHLESVEGYYDPKSDAIKGLQFKTNMRISELIGYANDGATKFSLAVEG 125

Query: 280 KK 285
           KK
Sbjct: 126 KK 127


>At1g08200.1 68414.m00906 expressed protein
          Length = 389

 Score = 29.1 bits (62), Expect = 1.4
 Identities = 21/83 (25%), Positives = 36/83 (43%)
 Frame = +1

Query: 175 SVFITGATGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMRKYLDQPIFSRIKYE 354
           ++ + GA GF+G    EKL    P  V    +  DK     E    ++  +  F RI  +
Sbjct: 19  TICMIGAGGFIGSHLCEKLMTETPHKVLALDVYNDKIKHLLEPDTVQWAGRIQFHRINIK 78

Query: 355 HPEYFKKIIPISGDITAPKLGLC 423
           H    + +I ++ D+T     +C
Sbjct: 79  HDSRLEGLIKMA-DLTINLAAIC 100


>At5g59450.1 68418.m07451 scarecrow-like transcription factor 11
           (SCL11) scarecrow-like 11, Arabidopsis thaliana,
           EMBL:AF036307
          Length = 610

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +1

Query: 55  LRKNCDQLQIIFKFRVISKMSHNGTLDE 138
           L++ CDQ  + F+F  I+K     TLDE
Sbjct: 388 LKRFCDQFNVPFEFNFIAKKWETITLDE 415


>At4g38850.1 68417.m05500 auxin-responsive protein / small auxin up
           RNA (SAUR-AC1) identical to GP:546362 small auxin up RNA
           {Arabidopsis thaliana}; belongs to auxin-induced
           (indole-3-acetic acid induced) protein family
          Length = 89

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 21/71 (29%), Positives = 37/71 (52%), Gaps = 2/71 (2%)
 Frame = +1

Query: 202 FLG-KAYVEKLAYSCP-GIVSIYILIRDKKGSNTEERMRKYLDQPIFSRIKYEHPEYFKK 375
           FLG K  + + + S P G +++Y+   D+K       +  YL+QP+F ++  +  E F  
Sbjct: 7   FLGAKQIIRRESSSTPRGFMAVYVGENDQKKKRYVVPV-SYLNQPLFQQLLSKSEEEFGY 65

Query: 376 IIPISGDITAP 408
             P+ G +T P
Sbjct: 66  DHPMGG-LTIP 75


>At2g34460.1 68415.m04229 flavin reductase-related low similarity to
           SP|P30043 Flavin reductase {Homo sapiens}
          Length = 280

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = +1

Query: 172 KSVFITGATGFLGKAYVEKL 231
           K VF+ GATG  GK  VE+L
Sbjct: 47  KKVFVAGATGQTGKRIVEQL 66


>At2g21280.1 68415.m02533 expressed protein similar to YfhF
           (GI:2804536) [Bacillus subtilis]
          Length = 347

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 10/19 (52%), Positives = 16/19 (84%)
 Frame = +1

Query: 175 SVFITGATGFLGKAYVEKL 231
           +V +TGATGF+G+  V++L
Sbjct: 47  TVSVTGATGFIGRRLVQRL 65


>At5g06060.1 68418.m00671 tropinone reductase, putative / tropine
           dehydrogenase, putative similar to tropinone reductase
           SP:P50165 from [Datura stramonium]
          Length = 264

 Score = 27.1 bits (57), Expect = 5.8
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = +1

Query: 169 GKSVFITGATGFLGKAYVEKLA 234
           GK+  +TG T  +G+A VE+LA
Sbjct: 11  GKTALVTGGTRGIGRAVVEELA 32


>At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein
           Similar to gb|U04299 mannosyl-oligosaccharide
           alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145
           and gb|AA394707 come from this gene
          Length = 574

 Score = 27.1 bits (57), Expect = 5.8
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = -2

Query: 242 HEYASFSTYAFPRNPVAPVIKT 177
           H Y ++ TYAFP + + P+ K+
Sbjct: 47  HAYDNYMTYAFPHDELKPLTKS 68


>At3g14790.1 68416.m01869 NAD-dependent epimerase/dehydratase family
           protein similar to dTDP-glucose 4,6-dehydratase from
           Aneurinibacillus thermoaerophilus GI:16357461,
           Saccharopolyspora spinosa GI:15077647, RmlB from
           Leptospira borgpetersenii GI:4234803; contains Pfam
           profile PF01370 NAD dependent epimerase/dehydratase
           family
          Length = 664

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 11/28 (39%), Positives = 15/28 (53%)
 Frame = +1

Query: 163 YDGKSVFITGATGFLGKAYVEKLAYSCP 246
           Y  K++ ITGA GF+      +L  S P
Sbjct: 4   YKPKNILITGAAGFIASHVANRLVRSYP 31


>At2g11623.1 68415.m01250 hypothetical protein
          Length = 110

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 9/24 (37%), Positives = 18/24 (75%)
 Frame = +1

Query: 304 RMRKYLDQPIFSRIKYEHPEYFKK 375
           ++  +L +PI+ +++ E PE+FKK
Sbjct: 72  KISHHLTKPIWEQLQKESPEFFKK 95


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,802,191
Number of Sequences: 28952
Number of extensions: 197713
Number of successful extensions: 491
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 478
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 485
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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