Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0002
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g49830.1 68418.m06171 expressed protein 43 2e-04
At1g10385.1 68414.m01170 hypothetical protein 40 0.001
At1g14460.1 68414.m01715 DNA polymerase-related weak similarity ... 30 1.2
At4g34131.1 68417.m04841 UDP-glucoronosyl/UDP-glucosyl transfera... 29 2.8
At5g38050.1 68418.m04585 hypothetical protein 29 3.8
At3g10180.1 68416.m01219 kinesin motor protein-related similar t... 29 3.8
At3g07920.1 68416.m00967 eukaryotic translation initiation facto... 28 5.0
At3g18090.1 68416.m02300 DNA-directed RNA polymerase family prot... 28 6.6
At5g66280.1 68418.m08357 GDP-D-mannose 4,6-dehydratase, putative... 27 8.7
At5g09820.1 68418.m01136 plastid-lipid associated protein PAP / ... 27 8.7
At3g23780.1 68416.m02989 DNA-directed RNA polymerase family prot... 27 8.7
At1g53830.1 68414.m06127 pectinesterase family protein identical... 27 8.7
>At5g49830.1 68418.m06171 expressed protein
Length = 752
Score = 43.2 bits (97), Expect = 2e-04
Identities = 24/78 (30%), Positives = 43/78 (55%)
Frame = +2
Query: 152 EIDMDKFAVPDFVPERYVKDLARSCVGGEELQQQKEKIQNLAEETASALKKNVYENYMQF 331
E + F F + YV+ ++ + ++++Q + +L +A ++++VY NY F
Sbjct: 21 EEGLSLFKSDKFDADAYVQ--SKCSINEKDIKQLCSYLLDLKRASAEEMRRSVYANYPAF 78
Query: 332 IETATEISHLETEMSSYR 385
I T+ EIS LE E+SS R
Sbjct: 79 IRTSKEISDLEGELSSIR 96
>At1g10385.1 68414.m01170 hypothetical protein
Length = 754
Score = 40.3 bits (90), Expect = 0.001
Identities = 22/67 (32%), Positives = 36/67 (53%)
Frame = +2
Query: 185 FVPERYVKDLARSCVGGEELQQQKEKIQNLAEETASALKKNVYENYMQFIETATEISHLE 364
F P+ YV + + +E + + L + +A ++K+VY NY FI T+ EIS LE
Sbjct: 37 FDPDAYVTSKCQR-MNEKETRHLSSYLVELKKASAEEMRKSVYANYAAFIRTSKEISALE 95
Query: 365 TEMSSYR 385
++ S R
Sbjct: 96 GQLLSMR 102
>At1g14460.1 68414.m01715 DNA polymerase-related weak similarity to
DNA polymerase III holoenzyme tau subunit [Thermus
thermophilus] GI:2583049
Length = 1116
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = +2
Query: 530 NLITEKVESCMNLLDVPDRTLLHEGDLLEIDAEENTGLQRMRVYL 664
+++TE+++ C + D L DLLE+DA + G +++R L
Sbjct: 483 DVVTEEMKPCGYCKECSDYMLGKSRDLLELDAGKKNGAEKVRYLL 527
>At4g34131.1 68417.m04841 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 481
Score = 29.1 bits (62), Expect = 2.8
Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = +2
Query: 182 DFVPERYVKDLARSCVGGEELQQQKEKIQNLAEETASALK-KNVYENYMQFIETAT 346
DF+ V R + GEE +++E+ + LAE +A++ + + + FIE T
Sbjct: 425 DFISREKVVKAVREVLVGEEADERRERAKKLAEMAKAAVEGGSSFNDLNSFIEEFT 480
>At5g38050.1 68418.m04585 hypothetical protein
Length = 281
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +1
Query: 253 EGENTKFSRRNRKCPKEKCLR---ELYAVYRDCDGDIPPRNRNVKLSHL 390
+GEN + + +R + KCLR E + R +PP + VK++H+
Sbjct: 100 DGENFRLEKLHRSVKRLKCLRVPDESASASRVVTKIVPPVEKPVKMAHI 148
>At3g10180.1 68416.m01219 kinesin motor protein-related similar to
centromere protein E GB:4502781 [Homo sapiens]
Length = 1348
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
Frame = +2
Query: 236 EELQQQKEKIQNLAEETASALKKNV---YENYMQFIETATEISHLETEMSSYR 385
EE+ +E+ Q E+ S + + EN+ + ATEI LE+E Y+
Sbjct: 639 EEIHAAREEAQVAREQLVSKESEVIDVINENFNSLVNVATEIEVLESEFQKYK 691
>At3g07920.1 68416.m00967 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 169
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 266 QNLAEETASALKKNVYENYMQFIET 340
Q L EETA KK V+ N+M + +T
Sbjct: 47 QVLREETAKGTKKTVFVNFMDYCKT 71
>At3g18090.1 68416.m02300 DNA-directed RNA polymerase family protein
similar to SP|P38420 DNA-directed RNA polymerase II 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
2) {Arabidopsis thaliana}; contains Pfam profiles
PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
domain 3, PF04566: RNA polymerase Rpb2 domain 4,
PF04567: RNA polymerase Rpb2 domain 5
Length = 1038
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +1
Query: 304 KCLRELYAVYRDCDGDIPPRNRNVKLSHLLSEQRSVLTVLSHA 432
KCL YA R C+ RN+ ++L+ L E R + L+HA
Sbjct: 246 KCLLSAYAGKRKCENRDSFRNKRIELAGELLE-REIRVHLAHA 287
>At5g66280.1 68418.m08357 GDP-D-mannose 4,6-dehydratase, putative
strong similarity to GDP-D-mannose-4,6-dehydratase
[Arabidopsis thaliana] GI:1764100
Length = 361
Score = 27.5 bits (58), Expect = 8.7
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = +1
Query: 70 YRIE*CASQCFVINTKEVYNKYMCNDV 150
Y CA+ + +N +E Y Y CN +
Sbjct: 173 YAASKCAAHWYTVNYREAYGLYACNGI 199
>At5g09820.1 68418.m01136 plastid-lipid associated protein PAP /
fibrillin family protein low similarity to plastid-lipid
associated protein PAP3 [Brassica rapa] GI:14248552;
contains Pfam profile PF04755: PAP_fibrillin
Length = 259
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 221 SCVGGEELQQQKEKIQNLAEETASALKKNVYE 316
SC E QQ +E+ Q E T S +K+ +YE
Sbjct: 61 SCSSPNEQQQDEEQEQEQEEITVSHIKEELYE 92
>At3g23780.1 68416.m02989 DNA-directed RNA polymerase family protein
similar to SP|P38420 DNA-directed RNA polymerase II 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
2) {Arabidopsis thaliana}; contains Pfam profiles
PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
domain 3, PF04566: RNA polymerase Rpb2 domain 4,
PF04567: RNA polymerase Rpb2 domain 5
Length = 946
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +1
Query: 304 KCLRELYAVYRDCDGDIPPRNRNVKLSHLLSEQRSVLTVLSHA 432
KCL YA R C+ RN+ ++L+ L E R + L+HA
Sbjct: 155 KCLLNSYAGKRKCENRDSFRNKRIELAGELLE-REIRVHLAHA 196
>At1g53830.1 68414.m06127 pectinesterase family protein identical to
pectinesterase 2 (PME2/ PE 2) SP:Q42534 from
[Arabidopsis thaliana];contains Pfam profiles: PF01095
pectinesterase, PF04043 plant invertase/pectin
methylesterase inhibitor
Length = 587
Score = 27.5 bits (58), Expect = 8.7
Identities = 19/66 (28%), Positives = 28/66 (42%)
Frame = +2
Query: 116 KKYTINTCATMSEIDMDKFAVPDFVPERYVKDLARSCVGGEELQQQKEKIQNLAEETASA 295
K I T ++ S + PE +A + GG+EL QKE I+ T A
Sbjct: 47 KNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAAT--GGKELTSQKEVIEASLNLTTKA 104
Query: 296 LKKNVY 313
+K N +
Sbjct: 105 VKHNYF 110
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,921,822
Number of Sequences: 28952
Number of extensions: 232358
Number of successful extensions: 666
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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