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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Nnor0476
         (492 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q5VV42 Cluster: CDK5 regulatory subunit-associated prot...   184   1e-45
UniRef50_Q7X7U6 Cluster: OSJNBa0088K19.13 protein; n=8; Viridipl...   144   1e-33
UniRef50_UPI00006CC448 Cluster: MiaB-like tRNA modifying enzyme,...   138   8e-32
UniRef50_Q5CXD5 Cluster: 2-methylthioadenine synthetase; MiaB; n...   119   4e-26
UniRef50_Q5VV42-2 Cluster: Isoform 2 of Q5VV42 ; n=3; Catarrhini...   116   4e-25
UniRef50_Q01CK2 Cluster: CDK5 activator-binding protein; n=1; Os...   114   9e-25
UniRef50_Q584Z1 Cluster: TRNA modification enzyme, putative; n=3...   113   2e-24
UniRef50_Q5C2M1 Cluster: SJCHGC07561 protein; n=1; Schistosoma j...   101   1e-20
UniRef50_Q4N1Y9 Cluster: Putative uncharacterized protein; n=2; ...   100   2e-20
UniRef50_O59545 Cluster: UPF0004 protein PH1875; n=5; Thermococc...    81   2e-14
UniRef50_Q91WE6-5 Cluster: Isoform 5 of Q91WE6 ; n=1; Mus muscul...    76   5e-13
UniRef50_Q7RQ12 Cluster: Drosophila melanogaster GH28477p-relate...    73   3e-12
UniRef50_Q6LF91 Cluster: Osjnba0088k19.13 protein; n=1; Plasmodi...    71   2e-11
UniRef50_Q4JA56 Cluster: Universally conserved protein; n=4; Sul...    71   2e-11
UniRef50_A5K256 Cluster: tRNA modifying enzyme, putative; n=1; P...    68   1e-10
UniRef50_A1RXU0 Cluster: RNA modification enzyme, MiaB family; n...    66   4e-10
UniRef50_Q9BKW0 Cluster: Putative uncharacterized protein; n=4; ...    63   4e-09
UniRef50_Q4SH97 Cluster: Chromosome 8 SCAF14587, whole genome sh...    62   7e-09
UniRef50_Q6ALW9 Cluster: Putative uncharacterized protein; n=1; ...    60   2e-08
UniRef50_A7DNS8 Cluster: MiaB-like tRNA modifying enzyme; n=2; C...    60   2e-08
UniRef50_Q1IQH5 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    60   3e-08
UniRef50_A5IJD4 Cluster: RNA modification enzyme, MiaB family; n...    60   3e-08
UniRef50_UPI00004984BC Cluster: RNA modification enzymes, MiaB-f...    59   6e-08
UniRef50_A0RW56 Cluster: 2-methylthioadenine synthetase; n=1; Ce...    58   8e-08
UniRef50_A1ZC85 Cluster: TRNA-I(6)A37 thiotransferase enzyme Mia...    58   1e-07
UniRef50_A0LFB7 Cluster: RNA modification enzyme, MiaB family; n...    57   2e-07
UniRef50_Q9L699 Cluster: UPF0004 protein PM1001; n=289; Proteoba...    57   2e-07
UniRef50_Q895H1 Cluster: MiaB protein; n=11; Bacteria|Rep: MiaB ...    56   3e-07
UniRef50_A4LYJ3 Cluster: TRNA-i(6)A37 thiotransferase enzyme Mia...    56   3e-07
UniRef50_Q8TWF4 Cluster: 2-methylthioadenine synthetase; n=1; Me...    56   3e-07
UniRef50_A7HAH8 Cluster: RNA modification enzyme, MiaB family; n...    56   4e-07
UniRef50_O26914 Cluster: UPF0004 protein MTH_826; n=3; Methanoba...    56   4e-07
UniRef50_Q8RA72 Cluster: 2-methylthioadenine synthetase; n=9; Cl...    56   6e-07
UniRef50_Q11BD9 Cluster: RNA modification enzyme, MiaB family; n...    56   6e-07
UniRef50_Q58277 Cluster: UPF0004 protein MJ0867; n=2; Methanococ...    56   6e-07
UniRef50_Q4HEV7 Cluster: MiaB-like tRNA modifying enzyme; n=19; ...    55   7e-07
UniRef50_Q2FSK8 Cluster: MiaB-like tRNA modifying enzyme; n=1; M...    55   7e-07
UniRef50_Q64CL1 Cluster: Putative uncharacterized protein; n=1; ...    55   7e-07
UniRef50_Q7QYP6 Cluster: GLP_393_20381_21958; n=1; Giardia lambl...    54   2e-06
UniRef50_Q74MF6 Cluster: NEQ008; n=1; Nanoarchaeum equitans|Rep:...    54   2e-06
UniRef50_A3DNI7 Cluster: RNA modification enzyme, MiaB family; n...    54   2e-06
UniRef50_Q2RZF8 Cluster: TRNA-i(6)A37 thiotransferase enzyme Mia...    54   2e-06
UniRef50_A7CVG2 Cluster: RNA modification enzyme, MiaB family pr...    54   2e-06
UniRef50_A3EV78 Cluster: 2-methylthioadenine synthetase; n=1; Le...    54   2e-06
UniRef50_O31778 Cluster: UPF0004 protein ymcB; n=55; Firmicutes|...    54   2e-06
UniRef50_A0B642 Cluster: MiaB-like tRNA modifying enzyme; n=1; M...    53   3e-06
UniRef50_Q74B44 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    53   4e-06
UniRef50_UPI00015B4592 Cluster: PREDICTED: similar to radical sa...    52   5e-06
UniRef50_Q2GCU4 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    52   5e-06
UniRef50_Q8TRM2 Cluster: 2-methylthioadenine synthase; n=4; Meth...    52   5e-06
UniRef50_Q55803 Cluster: UPF0004 protein slr0082; n=36; Cyanobac...    52   5e-06
UniRef50_A6DI62 Cluster: Putative uncharacterized protein; n=1; ...    52   7e-06
UniRef50_A0D7J9 Cluster: Chromosome undetermined scaffold_40, wh...    52   7e-06
UniRef50_Q8MXQ7 Cluster: CDKAL1-like protein; n=1; Caenorhabditi...    52   7e-06
UniRef50_A6DMH4 Cluster: Putative uncharacterized protein; n=1; ...    52   9e-06
UniRef50_A4SAH0 Cluster: Predicted protein; n=2; Ostreococcus|Re...    52   9e-06
UniRef50_Q7MAW4 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    51   1e-05
UniRef50_Q7ULM9 Cluster: Probable MiaB protein-putative tRNA-thi...    51   2e-05
UniRef50_Q2LQ68 Cluster: TRNA 2-methylthioadenine synthetase-lik...    51   2e-05
UniRef50_A4FZ90 Cluster: MiaB-like tRNA modifying enzyme; n=4; M...    51   2e-05
UniRef50_Q74A23 Cluster: MiaB-like tRNA modifying enzyme; n=3; D...    50   2e-05
UniRef50_O66638 Cluster: UPF0004 protein aq_284; n=2; Aquifex ae...    50   2e-05
UniRef50_Q8EUX4 Cluster: Putative uncharacterized protein MYPE79...    50   3e-05
UniRef50_P73127 Cluster: UPF0004 protein sll0996; n=37; Cyanobac...    50   3e-05
UniRef50_Q9WZT7 Cluster: UPF0004 protein TM_0830; n=2; Thermotog...    50   3e-05
UniRef50_Q6MAB7 Cluster: Probable 2-methylthioadenine synthetase...    50   4e-05
UniRef50_Q0AWM7 Cluster: MiaB-like tRNA modifying enzyme; n=1; S...    50   4e-05
UniRef50_A7H6G8 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    50   4e-05
UniRef50_Q49842 Cluster: UPF0004 protein ML0989; n=71; Actinobac...    50   4e-05
UniRef50_Q9ZCE8 Cluster: UPF0004 protein RP808; n=15; Alphaprote...    50   4e-05
UniRef50_A6PSP0 Cluster: RNA modification enzyme, MiaB family; n...    49   5e-05
UniRef50_A5UUG7 Cluster: RNA modification enzyme, MiaB family; n...    49   5e-05
UniRef50_A4XKJ7 Cluster: RNA modification enzyme, MiaB family; n...    49   5e-05
UniRef50_P56131 Cluster: UPF0004 protein HP_0269; n=26; Epsilonp...    49   5e-05
UniRef50_Q1V1E1 Cluster: TRNA-i(6)A37 modification enzyme; n=2; ...    49   7e-05
UniRef50_Q3AU39 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    48   9e-05
UniRef50_Q1PZS6 Cluster: Putative uncharacterized protein; n=1; ...    48   9e-05
UniRef50_Q2RJK1 Cluster: Putative uncharacterized protein; n=1; ...    48   1e-04
UniRef50_Q028J0 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    48   1e-04
UniRef50_A5D2R3 Cluster: 2-methylthioadenine synthetase; n=3; Cl...    48   1e-04
UniRef50_A4J5U4 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    48   1e-04
UniRef50_A4EC80 Cluster: Putative uncharacterized protein; n=1; ...    48   1e-04
UniRef50_A6NSZ3 Cluster: Putative uncharacterized protein; n=1; ...    48   2e-04
UniRef50_Q9YBR9 Cluster: MiaB homolog; n=2; Desulfurococcales|Re...    47   2e-04
UniRef50_Q823A0 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    47   3e-04
UniRef50_Q1JY65 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    47   3e-04
UniRef50_Q1FEI6 Cluster: Putative uncharacterized protein; n=2; ...    46   3e-04
UniRef50_Q3ZYS0 Cluster: TRNA-i(6)A37 thiotransferase enzyme Mia...    46   5e-04
UniRef50_A6GID8 Cluster: MiaB-like tRNA modifying enzyme YliG, T...    46   6e-04
UniRef50_A0L887 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    46   6e-04
UniRef50_A1HR14 Cluster: RNA modification enzyme, MiaB family; n...    45   8e-04
UniRef50_Q2AFA0 Cluster: Putative uncharacterized protein; n=1; ...    45   0.001
UniRef50_A6CGG9 Cluster: Probable MiaB protein-putative tRNA-thi...    45   0.001
UniRef50_Q5QP48 Cluster: CDK5 regulatory subunit associated prot...    45   0.001
UniRef50_Q0W344 Cluster: Putative 2-methylthioadenine synthetase...    45   0.001
UniRef50_Q96SZ6 Cluster: CDK5 regulatory subunit-associated prot...    45   0.001
UniRef50_Q54KV4 Cluster: Putative uncharacterized protein; n=1; ...    44   0.001
UniRef50_A7D1M3 Cluster: MiaB-like tRNA modifying enzyme; n=1; H...    44   0.001
UniRef50_O67016 Cluster: UPF0004 protein aq_849; n=2; Aquifex ae...    44   0.001
UniRef50_A6FYG6 Cluster: tRNA-i(6)A37 thiotransferase enzyme Mia...    44   0.002
UniRef50_A6DR68 Cluster: Putative Fe-S oxidoreductase; n=1; Lent...    44   0.002
UniRef50_A7I5K8 Cluster: MiaB-like tRNA modifying enzyme; n=1; C...    44   0.002
UniRef50_Q09316 Cluster: CDK5RAP1-like protein; n=3; Bilateria|R...    44   0.002
UniRef50_Q6A908 Cluster: Conserved protein, radical SAM superfam...    44   0.002
UniRef50_A6LKT7 Cluster: MiaB-like tRNA modifying enzyme; n=2; T...    44   0.002
UniRef50_Q1IPQ5 Cluster: Putative uncharacterized protein; n=1; ...    43   0.003
UniRef50_A6GE00 Cluster: tRNA 2-methylthioadenosine synthase-lik...    43   0.003
UniRef50_A5UQQ2 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    43   0.003
UniRef50_UPI00006CFA0B Cluster: RNA modification enzyme, MiaB fa...    43   0.004
UniRef50_Q8H0V1 Cluster: CDK5RAP1-like protein; n=9; Viridiplant...    43   0.004
UniRef50_Q8RB61 Cluster: 2-methylthioadenine synthetase; n=19; C...    42   0.006
UniRef50_Q67NX5 Cluster: 2-methylthioadenine synthetase; n=1; Sy...    42   0.006
UniRef50_Q1VHX9 Cluster: Putative uncharacterized protein; n=1; ...    42   0.006
UniRef50_A7B2V4 Cluster: Putative uncharacterized protein; n=1; ...    42   0.006
UniRef50_A6P2W1 Cluster: Putative uncharacterized protein; n=1; ...    42   0.006
UniRef50_A1I9T0 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    42   0.006
UniRef50_A2SQZ8 Cluster: MiaB-like tRNA modifying enzyme; n=1; M...    42   0.006
UniRef50_Q9VGZ1 Cluster: CDK5RAP1-like protein; n=2; Sophophora|...    42   0.006
UniRef50_A5ZQ90 Cluster: Putative uncharacterized protein; n=2; ...    42   0.007
UniRef50_Q6MAB2 Cluster: Putative 2-methylthioadenine synthetase...    42   0.010
UniRef50_Q3ACX5 Cluster: MiaB-like tRNA modifying enzyme YliG, T...    42   0.010
UniRef50_Q892R4 Cluster: Fe-S oxidoreductase; n=3; Clostridium|R...    41   0.013
UniRef50_Q1JYQ2 Cluster: MiaB-like tRNA modifying enzyme; n=2; D...    41   0.013
UniRef50_A6ESE6 Cluster: Possible 2-methylthioadenine synthetase...    41   0.013
UniRef50_O83735 Cluster: UPF0004 protein TP_0754; n=2; Treponema...    41   0.013
UniRef50_Q2LVR5 Cluster: TRNA 2-methylthioadenosine synthase-lik...    41   0.017
UniRef50_Q2J750 Cluster: Putative uncharacterized protein; n=2; ...    41   0.017
UniRef50_Q04UA3 Cluster: 2-methylthioadenine synthetase; n=4; Le...    41   0.017
UniRef50_A0LV11 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    41   0.017
UniRef50_Q6MLC6 Cluster: Putative uncharacterized protein; n=1; ...    40   0.023
UniRef50_Q6AQ27 Cluster: Putative uncharacterized protein; n=3; ...    40   0.023
UniRef50_Q6AIZ5 Cluster: Putative uncharacterized protein; n=1; ...    40   0.023
UniRef50_Q3A8J5 Cluster: 2-methylthioadenine synthetase; n=2; De...    40   0.023
UniRef50_Q1ISD7 Cluster: MiaB-like tRNA modifying enzyme; n=2; A...    40   0.023
UniRef50_A7CWE3 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    40   0.023
UniRef50_A5GE34 Cluster: MiaB-like tRNA modifying enzyme; n=5; D...    40   0.023
UniRef50_Q73JG6 Cluster: MiaB-like tRNA modifying enzyme YliG, T...    40   0.030
UniRef50_Q1AW39 Cluster: Putative uncharacterized protein; n=1; ...    40   0.030
UniRef50_A7GZE8 Cluster: 2-methylthioadenine synthetase; n=14; E...    40   0.030
UniRef50_A5TX86 Cluster: tRNA 2-methylthioadenosine synthase; n=...    40   0.030
UniRef50_A1VF04 Cluster: RNA modification enzyme, MiaB family; n...    40   0.030
UniRef50_Q01DS1 Cluster: Predicted Fe-S oxidoreductase; n=1; Ost...    40   0.030
UniRef50_A0UWB9 Cluster: Radical SAM; n=1; Clostridium celluloly...    40   0.040
UniRef50_Q6L1Y8 Cluster: Hypothetical oxidoreductase; n=4; Therm...    40   0.040
UniRef50_UPI00015BB1B3 Cluster: RNA modification enzyme, MiaB fa...    39   0.053
UniRef50_A6C349 Cluster: Putative uncharacterized protein; n=1; ...    39   0.053
UniRef50_Q5SHW2 Cluster: Putative uncharacterized protein TTHA16...    39   0.070
UniRef50_Q1PXT1 Cluster: Putative uncharacterized protein; n=1; ...    39   0.070
UniRef50_Q9HP07 Cluster: Putative uncharacterized protein; n=3; ...    39   0.070
UniRef50_A1IFA3 Cluster: TRNA 2-methylthioadenosine synthase-lik...    38   0.092
UniRef50_P54462 Cluster: UPF0004 protein yqeV; n=38; Bacillales|...    38   0.092
UniRef50_Q6MGT1 Cluster: Putative uncharacterized protein; n=1; ...    38   0.12 
UniRef50_Q607P8 Cluster: Putative uncharacterized protein; n=1; ...    38   0.12 
UniRef50_A6QCC6 Cluster: tRNA modifying enzyme; n=3; Epsilonprot...    38   0.12 
UniRef50_A5TU09 Cluster: 2-methylthioadenine synthetase; n=3; Fu...    38   0.12 
UniRef50_A0W5N6 Cluster: MiaB-like tRNA modifying enzyme; n=1; G...    38   0.12 
UniRef50_A0LIM0 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    38   0.12 
UniRef50_A5FQT7 Cluster: MiaB-like tRNA modifying enzyme; n=3; D...    38   0.16 
UniRef50_A1IDX9 Cluster: TRNA-i(6)A37 modification enzyme MiaB; ...    38   0.16 
UniRef50_Q1Q4S9 Cluster: Similar to 2-methylthioadenine syntheta...    37   0.21 
UniRef50_Q04PJ5 Cluster: 2-methylthioadenine synthetase; n=4; Le...    37   0.21 
UniRef50_A4S5H4 Cluster: Predicted protein; n=2; Ostreococcus|Re...    37   0.21 
UniRef50_P56130 Cluster: UPF0004 protein HP_0285; n=10; Epsilonp...    37   0.21 
UniRef50_A7H5G3 Cluster: MiaB-like tRNA modifying enzyme YliG, T...    37   0.28 
UniRef50_A5ZXQ4 Cluster: Putative uncharacterized protein; n=1; ...    37   0.28 
UniRef50_A4XLD9 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    37   0.28 
UniRef50_A4M7C8 Cluster: MiaB-like tRNA modifying enzyme; n=1; P...    37   0.28 
UniRef50_Q7UK39 Cluster: Putative uncharacterized protein; n=2; ...    36   0.37 
UniRef50_Q49573 Cluster: UPF0004 protein in 16S RNA 5'region; n=...    36   0.37 
UniRef50_Q2RKX1 Cluster: MiaB-like tRNA modifying enzyme; n=5; C...    36   0.49 
UniRef50_Q7MSY9 Cluster: MiaB-like tRNA modifying enzyme; n=4; B...    35   0.86 
UniRef50_Q4W554 Cluster: MiaB-like tRNA modifying enzyme; n=6; C...    35   0.86 
UniRef50_Q30XS8 Cluster: Putative uncharacterized protein; n=1; ...    35   1.1  
UniRef50_Q194H8 Cluster: TRNA-i(6)A37 thiotransferase enzyme Mia...    35   1.1  
UniRef50_Q0AXI3 Cluster: 2-methylthioadenine synthetase; n=1; Sy...    35   1.1  
UniRef50_Q03HM3 Cluster: Transcriptional regulator containing an...    35   1.1  
UniRef50_A3JF75 Cluster: Putative uncharacterized protein; n=1; ...    35   1.1  
UniRef50_A1FEK1 Cluster: Putative uncharacterized protein; n=1; ...    35   1.1  
UniRef50_O66772 Cluster: UPF0004 protein aq_474; n=1; Aquifex ae...    35   1.1  
UniRef50_Q29R15 Cluster: LP17019p; n=5; Sophophora|Rep: LP17019p...    34   1.5  
UniRef50_Q4RNH8 Cluster: Chromosome undetermined SCAF15013, whol...    34   2.0  
UniRef50_Q1NYL6 Cluster: TRNA-i(6)A37 thiotransferase enzyme Mia...    34   2.0  
UniRef50_UPI00015BD265 Cluster: UPI00015BD265 related cluster; n...    33   2.6  
UniRef50_Q9RYW7 Cluster: Putative uncharacterized protein; n=1; ...    33   2.6  
UniRef50_Q04ZD0 Cluster: 2-methylthioadenine synthetase; n=5; Le...    33   2.6  
UniRef50_A5GAH4 Cluster: Metallophosphoesterase precursor; n=1; ...    33   2.6  
UniRef50_A3ZYE3 Cluster: Putative uncharacterized protein; n=2; ...    33   2.6  
UniRef50_A0M3K8 Cluster: Radical SAM superfamily protein, UPF000...    33   2.6  
UniRef50_Q0YRY0 Cluster: MiaB-like tRNA modifying enzyme; n=4; C...    33   3.5  
UniRef50_A4M7N1 Cluster: MiaB-like tRNA modifying enzyme YliG; n...    33   3.5  
UniRef50_A3EVU0 Cluster: 2-methylthioadenine synthetase; n=1; Le...    33   3.5  
UniRef50_A0JCT4 Cluster: 5' nucleotidase, putative; n=1; Glyptap...    33   3.5  
UniRef50_Q9CKN9 Cluster: UPF0004 protein PM1571; n=239; cellular...    33   3.5  
UniRef50_UPI000155FF6B Cluster: PREDICTED: similar to lymphocyte...    33   4.6  
UniRef50_A6NW35 Cluster: Putative uncharacterized protein; n=1; ...    33   4.6  
UniRef50_A6R7E1 Cluster: Predicted protein; n=1; Ajellomyces cap...    33   4.6  
UniRef50_Q1FGL7 Cluster: MiaB-like tRNA modifying enzyme; n=5; C...    32   6.0  
UniRef50_Q2U1C1 Cluster: Predicted protein; n=1; Aspergillus ory...    32   6.0  
UniRef50_Q8PKB2 Cluster: Putative uncharacterized protein XAC226...    32   8.0  
UniRef50_A0LEL6 Cluster: RNA modification enzyme, MiaB family; n...    32   8.0  

>UniRef50_Q5VV42 Cluster: CDK5 regulatory subunit-associated protein
           1-like 1; n=48; Eumetazoa|Rep: CDK5 regulatory
           subunit-associated protein 1-like 1 - Homo sapiens
           (Human)
          Length = 579

 Score =  184 bits (448), Expect = 1e-45
 Identities = 85/149 (57%), Positives = 109/149 (73%), Gaps = 2/149 (1%)
 Frame = +2

Query: 50  PKERYASRKNV--SVRSKKREKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYM 223
           P++R+  RK+V   VR +  +K   E+      +S +PG Q I+++TWGC+HNNSD EYM
Sbjct: 24  PQDRHFVRKDVVPKVRRRNTQKYLQEEENSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYM 83

Query: 224 AGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQGA 403
           AG LAA GYK+TE+  DA LWLLNSCTVK+PAEDHF+N I+  Q     +V+AGCVPQ  
Sbjct: 84  AGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKIVLAGCVPQAQ 143

Query: 404 PKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
           P+  YL GLSI+GVQQIDR+VEVVE T+K
Sbjct: 144 PRQDYLKGLSIIGVQQIDRVVEVVEETIK 172


>UniRef50_Q7X7U6 Cluster: OSJNBa0088K19.13 protein; n=8;
           Viridiplantae|Rep: OSJNBa0088K19.13 protein - Oryza
           sativa subsp. japonica (Rice)
          Length = 626

 Score =  144 bits (348), Expect = 1e-33
 Identities = 67/116 (57%), Positives = 87/116 (75%)
 Frame = +2

Query: 143 ESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           E+ +PGTQTIYVKT+GC+HN SDSEYM+G L+A GY +TE+   A LWL+N+CTVK+P++
Sbjct: 51  EARIPGTQTIYVKTFGCSHNQSDSEYMSGQLSAFGYAITEEPEGADLWLINTCTVKNPSQ 110

Query: 323 DHFKNEIELGQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
                 I   +S    +VVAGCVPQG+     L G+S++GVQQIDR+VEVVE TLK
Sbjct: 111 SAMTTLISKCKSANKPLVVAGCVPQGSRDLKELEGISVIGVQQIDRVVEVVEETLK 166


>UniRef50_UPI00006CC448 Cluster: MiaB-like tRNA modifying enzyme,
           archaeal-type family protein; n=1; Tetrahymena
           thermophila SB210|Rep: MiaB-like tRNA modifying enzyme,
           archaeal-type family protein - Tetrahymena thermophila
           SB210
          Length = 574

 Score =  138 bits (333), Expect = 8e-32
 Identities = 70/150 (46%), Positives = 97/150 (64%), Gaps = 4/150 (2%)
 Frame = +2

Query: 53  KERYASRKNVSVRSKKREKKDPEQIEKVILE----SVVPGTQTIYVKTWGCAHNNSDSEY 220
           K+R    K V    K+ E ++PE  +++  +    + VPGTQ +YVKT+GC+HN SDSE+
Sbjct: 33  KKRPKKVKKVEEEPKQEELQEPEDDDEIKFDMPVNNQVPGTQNVYVKTFGCSHNISDSEF 92

Query: 221 MAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQG 400
           M G LA  GY L  D  DA L L+NSCTVK+P++D F   ++  + +   +VVAGCVPQG
Sbjct: 93  MMGQLAEYGYNLCSDPKDAHLILVNSCTVKNPSQDAFMTIVKTYKHKKKPIVVAGCVPQG 152

Query: 401 APKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
                 L  +S++G+ QIDR+VEVVE TLK
Sbjct: 153 DRNIPGLEDVSVIGISQIDRVVEVVEETLK 182


>UniRef50_Q5CXD5 Cluster: 2-methylthioadenine synthetase; MiaB; n=3;
           Cryptosporidium|Rep: 2-methylthioadenine synthetase;
           MiaB - Cryptosporidium parvum Iowa II
          Length = 543

 Score =  119 bits (286), Expect = 4e-26
 Identities = 60/116 (51%), Positives = 76/116 (65%)
 Frame = +2

Query: 143 ESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           E  VPG   I VK +GC HN SDSE M GLL+  GY L E+  +  L ++NSCTVK P++
Sbjct: 95  EGFVPGVAKIMVKNFGCNHNRSDSESMMGLLSEYGYTLVEELDECNLIVINSCTVKGPSQ 154

Query: 323 DHFKNEIELGQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
           D  +N IEL +S+   VVV GCVPQ      +L  +SI+GV+ I RIVEVVE TL+
Sbjct: 155 DSCQNLIELAKSKRKFVVVTGCVPQADINLNFLKDVSIIGVRNIHRIVEVVELTLQ 210


>UniRef50_Q5VV42-2 Cluster: Isoform 2 of Q5VV42 ; n=3;
           Catarrhini|Rep: Isoform 2 of Q5VV42 - Homo sapiens
           (Human)
          Length = 488

 Score =  116 bits (278), Expect = 4e-25
 Identities = 51/81 (62%), Positives = 64/81 (79%)
 Frame = +2

Query: 248 YKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQGAPKSGYLHG 427
           +++TE+  DA LWLLNSCTVK+PAEDHF+N I+  Q     +V+AGCVPQ  P+  YL G
Sbjct: 22  HQVTENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKIVLAGCVPQAQPRQDYLKG 81

Query: 428 LSIVGVQQIDRIVEVVEXTLK 490
           LSI+GVQQIDR+VEVVE T+K
Sbjct: 82  LSIIGVQQIDRVVEVVEETIK 102


>UniRef50_Q01CK2 Cluster: CDK5 activator-binding protein; n=1;
           Ostreococcus tauri|Rep: CDK5 activator-binding protein -
           Ostreococcus tauri
          Length = 558

 Score =  114 bits (275), Expect = 9e-25
 Identities = 52/106 (49%), Positives = 76/106 (71%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           I+V T+GC+HN+SDSE+MAG L + GY+L +D  DA  WL+N+CTVK+P++      +E 
Sbjct: 35  IFVHTFGCSHNHSDSEFMAGQLQSYGYELVKDASDADGWLVNTCTVKNPSQSAMNTVLER 94

Query: 350 GQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTL 487
           G++    ++VAGCVPQG   +  L  +S++GV QIDR+VE +E TL
Sbjct: 95  GKAANKALLVAGCVPQGDKGAKELKDVSLLGVTQIDRVVEAMERTL 140


>UniRef50_Q584Z1 Cluster: TRNA modification enzyme, putative; n=3;
           Trypanosoma|Rep: TRNA modification enzyme, putative -
           Trypanosoma brucei
          Length = 535

 Score =  113 bits (272), Expect = 2e-24
 Identities = 52/113 (46%), Positives = 75/113 (66%)
 Frame = +2

Query: 152 VPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHF 331
           +PG  TI+V T+GC HN SD EYMAG L  +GY +T++   A  +LLNSCTVK+P+E+HF
Sbjct: 47  IPGNATIFVHTFGCGHNVSDGEYMAGQLVESGYNVTDEFGQADAYLLNSCTVKNPSEEHF 106

Query: 332 KNEIELGQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
            + +   +  G  ++VAGCVPQ  P +     +S+VGV+ ID +  VV+  L+
Sbjct: 107 VSMMNRVRDTGKPLIVAGCVPQADPTNKQWGDVSVVGVRSIDCVSYVVQEALQ 159


>UniRef50_Q5C2M1 Cluster: SJCHGC07561 protein; n=1; Schistosoma
           japonicum|Rep: SJCHGC07561 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 218

 Score =  101 bits (241), Expect = 1e-20
 Identities = 54/104 (51%), Positives = 63/104 (60%), Gaps = 4/104 (3%)
 Frame = +2

Query: 191 CAHNNSDSEYMAGLLAANGYKLTE----DKWDAQLWLLNSCTVKSPAEDHFKNEIELGQS 358
           C  NN D E  +G    N  K        K  A +W+LNSCTVK PAEDHF+N +  G  
Sbjct: 103 CQRNNDD-ECCSGERILNRRKDMSPHFNSKMKADIWVLNSCTVKGPAEDHFRNAVLEGLK 161

Query: 359 RGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
            G  VV  GCVPQ  P + YL G+S+VGV QIDRIVEVVE TL+
Sbjct: 162 LGKRVVACGCVPQSRPGADYLKGVSVVGVHQIDRIVEVVEETLQ 205



 Score = 63.7 bits (148), Expect = 2e-09
 Identities = 34/71 (47%), Positives = 47/71 (66%), Gaps = 1/71 (1%)
 Frame = +2

Query: 56  ERYASRKNVSVRSKKREKKDPEQI-EKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGL 232
           +R  +   V V++K R KK  +QI + + L S +P    I+V+TWGCAHN SDSEYM GL
Sbjct: 11  DRPETVSTVLVKTKFRNKK--QQISDDLCLSSYLPERFHIFVQTWGCAHNTSDSEYMTGL 68

Query: 233 LAANGYKLTED 265
           LA  G+++T D
Sbjct: 69  LAKYGFQVTLD 79


>UniRef50_Q4N1Y9 Cluster: Putative uncharacterized protein; n=2;
           Theileria|Rep: Putative uncharacterized protein -
           Theileria parva
          Length = 750

 Score =  100 bits (240), Expect = 2e-20
 Identities = 46/116 (39%), Positives = 72/116 (62%), Gaps = 2/116 (1%)
 Frame = +2

Query: 146 SVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED 325
           S+ PG   +Y+K +GC+HN SDSEYM G+++ +GY +T+      L ++NSCTVK+P+E 
Sbjct: 320 SINPGEVVVYLKNFGCSHNISDSEYMLGIISESGYAITDTMDSCDLVIINSCTVKNPSEH 379

Query: 326 HFKNEIELGQSRGIHVVVAGCVPQGAPKSGYL--HGLSIVGVQQIDRIVEVVEXTL 487
              N I  G   G  ++V GC+PQ          + +S++G+ QI++IV V+E  L
Sbjct: 380 GMINYINQGLKLGKKIIVTGCIPQSDKLHPIFNNNNISLLGIMQIEKIVYVIENML 435


>UniRef50_O59545 Cluster: UPF0004 protein PH1875; n=5;
           Thermococcaceae|Rep: UPF0004 protein PH1875 - Pyrococcus
           horikoshii
          Length = 425

 Score = 80.6 bits (190), Expect = 2e-14
 Identities = 38/107 (35%), Positives = 61/107 (57%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y++ +GCA N +D E MA LL  +G+++ E   ++++ ++NSC VK P E      I  
Sbjct: 4   VYIENYGCARNRADGEIMAALLYLSGHEIVESPEESEIVVVNSCAVKDPTERKIARRIRE 63

Query: 350 GQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
               G  V+V GC+P   P        +I+GV+ IDRIV+ VE  ++
Sbjct: 64  LLDNGKKVIVTGCLPHVNPDVIDERVSAILGVKSIDRIVQAVEYAMR 110


>UniRef50_Q91WE6-5 Cluster: Isoform 5 of Q91WE6 ; n=1; Mus
           musculus|Rep: Isoform 5 of Q91WE6 - Mus musculus (Mouse)
          Length = 136

 Score = 75.8 bits (178), Expect = 5e-13
 Identities = 35/71 (49%), Positives = 50/71 (70%), Gaps = 1/71 (1%)
 Frame = +2

Query: 50  PKERYASRKNVSVRSKKREKKDPEQIE-KVILESVVPGTQTIYVKTWGCAHNNSDSEYMA 226
           P++R  SRK+V  + ++R  +   Q E +   +S +PG Q I+++TWGC+HNNSD EYMA
Sbjct: 24  PQDRQFSRKHVFPKVRRRNTQKYLQEEPRPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMA 83

Query: 227 GLLAANGYKLT 259
           G LAA GYK+T
Sbjct: 84  GQLAAYGYKIT 94


>UniRef50_Q7RQ12 Cluster: Drosophila melanogaster GH28477p-related;
           n=4; Plasmodium (Vinckeia)|Rep: Drosophila melanogaster
           GH28477p-related - Plasmodium yoelii yoelii
          Length = 817

 Score = 72.9 bits (171), Expect = 3e-12
 Identities = 30/65 (46%), Positives = 46/65 (70%)
 Frame = +2

Query: 149 VVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDH 328
           ++P    IY K++GCAHN+SDSE+M GLL+  G+K  ++  D  + ++NSCTVK+P+E+ 
Sbjct: 246 IIPENYNIYFKSFGCAHNSSDSEFMMGLLSNYGFKFVKNIEDCDICIVNSCTVKNPSEES 305

Query: 329 FKNEI 343
            K  I
Sbjct: 306 MKTII 310



 Score = 40.7 bits (91), Expect = 0.017
 Identities = 23/64 (35%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
 Frame = +2

Query: 305 VKSPAEDHFKNEI---ELGQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVV 475
           VK+  E    NEI        + I ++V GCVPQ          +S+VGV  ID+IV+ V
Sbjct: 433 VKNKVEG-INNEIIKKRTNSGKDIKIIVCGCVPQAENDMKIFENVSLVGVNNIDKIVDAV 491

Query: 476 EXTL 487
           E  +
Sbjct: 492 ENVI 495


>UniRef50_Q6LF91 Cluster: Osjnba0088k19.13 protein; n=1; Plasmodium
           falciparum 3D7|Rep: Osjnba0088k19.13 protein -
           Plasmodium falciparum (isolate 3D7)
          Length = 860

 Score = 70.5 bits (165), Expect = 2e-11
 Identities = 30/65 (46%), Positives = 45/65 (69%)
 Frame = +2

Query: 149 VVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDH 328
           ++P    IY K++GCAHN+SDSE+M GLLA  G+K  +   +  + ++NSCTVK+P+E+ 
Sbjct: 238 ILPENYKIYFKSFGCAHNSSDSEFMMGLLANYGFKFVKKIEECDICIVNSCTVKNPSEES 297

Query: 329 FKNEI 343
            K  I
Sbjct: 298 MKTII 302



 Score = 45.2 bits (102), Expect = 8e-04
 Identities = 22/65 (33%), Positives = 36/65 (55%)
 Frame = +2

Query: 293 NSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEV 472
           N+      +E + K +I + + + I ++V GCVPQ          +S+VGV  ID+IV+V
Sbjct: 474 NNILENRTSEKNKKKKIHV-EGKNIKIIVCGCVPQAEKDMEIFENVSLVGVNNIDKIVDV 532

Query: 473 VEXTL 487
           VE  +
Sbjct: 533 VENVI 537


>UniRef50_Q4JA56 Cluster: Universally conserved protein; n=4;
           Sulfolobaceae|Rep: Universally conserved protein -
           Sulfolobus acidocaldarius
          Length = 421

 Score = 70.5 bits (165), Expect = 2e-11
 Identities = 36/106 (33%), Positives = 60/106 (56%), Gaps = 3/106 (2%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-E 346
           +Y++T+GCA N  DS  M  LL   G+++ ++  DA++ ++N+C V+   E+  K  I E
Sbjct: 3   VYIETYGCALNKGDSYIMMTLLRDKGHEIVDNIQDAEILVINTCAVRLETEERMKQRIKE 62

Query: 347 LGQSRGIHVVVAGCVPQGAPK--SGYLHGLSIVGVQQIDRIVEVVE 478
           L +     +VVAGC+    P          S++G Q + +IV+VVE
Sbjct: 63  LKKYNDKRLVVAGCLASAEPAVVVSLAPEASVIGPQSVQKIVDVVE 108


>UniRef50_A5K256 Cluster: tRNA modifying enzyme, putative; n=1;
           Plasmodium vivax|Rep: tRNA modifying enzyme, putative -
           Plasmodium vivax
          Length = 799

 Score = 68.1 bits (159), Expect = 1e-10
 Identities = 28/65 (43%), Positives = 44/65 (67%)
 Frame = +2

Query: 149 VVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDH 328
           ++P    IY K++GCAHN+SDSE+M GLL   G++  +   +  + ++NSCTVK+P+E+ 
Sbjct: 251 ILPEKYKIYFKSFGCAHNSSDSEFMMGLLGNYGFQFVKSVEECDICIINSCTVKNPSEES 310

Query: 329 FKNEI 343
            K  I
Sbjct: 311 MKTII 315



 Score = 41.5 bits (93), Expect = 0.010
 Identities = 18/41 (43%), Positives = 25/41 (60%)
 Frame = +2

Query: 365 IHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTL 487
           I ++V GCVPQ          +S+VGV  ID+IV+VVE  +
Sbjct: 437 IKIIVCGCVPQAEKDMEIFENVSLVGVTNIDKIVDVVENVI 477


>UniRef50_A1RXU0 Cluster: RNA modification enzyme, MiaB family; n=1;
           Thermofilum pendens Hrk 5|Rep: RNA modification enzyme,
           MiaB family - Thermofilum pendens (strain Hrk 5)
          Length = 428

 Score = 66.1 bits (154), Expect = 4e-10
 Identities = 39/109 (35%), Positives = 58/109 (53%), Gaps = 6/109 (5%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-- 343
           +Y++T+GC  N  +S  MA LL   G+K+ E   +A + +LN+C V+   E      +  
Sbjct: 4   VYIETFGCWLNKGESNIMATLLKRRGHKVVESIENADVVILNTCAVRGDTETKIFRRLRE 63

Query: 344 --ELGQSRGIHVVVAGCVPQGAPKS--GYLHGLSIVGVQQIDRIVEVVE 478
             EL Q RG  +VV+GC+    PKS        S+V    I++I EVVE
Sbjct: 64  LEELRQKRGFRLVVSGCLVNVRPKSILDVAPSASLVEPDAIEKIPEVVE 112


>UniRef50_Q9BKW0 Cluster: Putative uncharacterized protein; n=4;
           Caenorhabditis|Rep: Putative uncharacterized protein -
           Caenorhabditis elegans
          Length = 397

 Score = 62.9 bits (146), Expect = 4e-09
 Identities = 27/62 (43%), Positives = 45/62 (72%), Gaps = 1/62 (1%)
 Frame = +2

Query: 80  VSVRSKKREKKDPEQIEKVILESVVPGT-QTIYVKTWGCAHNNSDSEYMAGLLAANGYKL 256
           + +R++K+  K+ +Q +   ++S+VPG  Q ++V+TWGC+HN SDSEYM+GLL   GY +
Sbjct: 20  IKIRTRKQVPKE-QQPDDANVDSMVPGVGQKVWVRTWGCSHNTSDSEYMSGLLQQAGYDV 78

Query: 257 TE 262
            +
Sbjct: 79  VK 80


>UniRef50_Q4SH97 Cluster: Chromosome 8 SCAF14587, whole genome
           shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
           Chromosome 8 SCAF14587, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 253

 Score = 62.1 bits (144), Expect = 7e-09
 Identities = 27/40 (67%), Positives = 33/40 (82%)
 Frame = +2

Query: 371 VVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
           VV+AGCVPQ  P+  YL GLSI+GVQQIDR+VEVV+  +K
Sbjct: 102 VVLAGCVPQAQPRMDYLKGLSIIGVQQIDRVVEVVDEAIK 141



 Score = 51.6 bits (118), Expect = 9e-06
 Identities = 22/36 (61%), Positives = 27/36 (75%)
 Frame = +2

Query: 239 ANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           A   K  +D  +A LWLLNSCTVK+PAEDHF+N I+
Sbjct: 9   AEDRKNRDDPIEADLWLLNSCTVKNPAEDHFRNSIK 44


>UniRef50_Q6ALW9 Cluster: Putative uncharacterized protein; n=1;
           Desulfotalea psychrophila|Rep: Putative uncharacterized
           protein - Desulfotalea psychrophila
          Length = 447

 Score = 60.5 bits (140), Expect = 2e-08
 Identities = 39/113 (34%), Positives = 65/113 (57%), Gaps = 8/113 (7%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           ++ ++KT+GC  N  DSE +A +L  NGY  T +   A L LLN+C++++ AE    +++
Sbjct: 4   RSFFIKTYGCQMNLRDSEIIAQILNNNGYVETSEIGGADLVLLNTCSIRAKAEQKVMSKL 63

Query: 344 -ELGQSRGIH----VVVAGCVPQGAPK---SGYLHGLSIVGVQQIDRIVEVVE 478
            EL +++ I+    + VAGCV Q   K   +   H   ++G Q I  I E++E
Sbjct: 64  GELRRNKKINPRMQICVAGCVAQQEGKQIQAKMPHVDLVIGTQYIYAINELLE 116


>UniRef50_A7DNS8 Cluster: MiaB-like tRNA modifying enzyme; n=2;
           Crenarchaeota|Rep: MiaB-like tRNA modifying enzyme -
           Candidatus Nitrosopumilus maritimus SCM1
          Length = 422

 Score = 60.5 bits (140), Expect = 2e-08
 Identities = 30/109 (27%), Positives = 67/109 (61%), Gaps = 2/109 (1%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           I+V+++GC+ + +DSE ++GL+   G+ L ED  ++ L ++ +C+VK    +   + I+ 
Sbjct: 4   IFVESYGCSASFADSEMISGLILNGGHTLVEDSSESDLNVVVTCSVKDATANKMVHRIKS 63

Query: 350 GQSRGIHVVVAGCVPQGAPKS--GYLHGLSIVGVQQIDRIVEVVEXTLK 490
            +++ +  VVAGC+P+   ++   +    S++G   + + ++V++ TLK
Sbjct: 64  LKTKPL--VVAGCLPKAEKETVEKFSENASLLGPNSLGKTLQVIDSTLK 110


>UniRef50_Q1IQH5 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=2; Acidobacteria|Rep: TRNA-i(6)A37 modification enzyme
           MiaB - Acidobacteria bacterium (strain Ellin345)
          Length = 444

 Score = 60.1 bits (139), Expect = 3e-08
 Identities = 31/81 (38%), Positives = 44/81 (54%), Gaps = 3/81 (3%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED---HFK 334
           +T Y++T+GC  N  DSE + G L + GY+  E + DA L L N+C+++  AE    H  
Sbjct: 8   KTFYIETFGCQMNFHDSEKVVGTLISQGYRQVETELDAGLILYNTCSIRDKAEQKVFHRL 67

Query: 335 NEIELGQSRGIHVVVAGCVPQ 397
           +E    Q  G    V GCV Q
Sbjct: 68  SEFRQLQKEGKRFAVLGCVAQ 88


>UniRef50_A5IJD4 Cluster: RNA modification enzyme, MiaB family; n=5;
           Thermotogaceae|Rep: RNA modification enzyme, MiaB family
           - Thermotoga petrophila RKU-1
          Length = 443

 Score = 59.7 bits (138), Expect = 3e-08
 Identities = 33/111 (29%), Positives = 61/111 (54%), Gaps = 5/111 (4%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE---- 340
           Y+KT+GC  N +DSE MAGLL   G+       +A + ++N+C V+  +E+   +E    
Sbjct: 4   YIKTFGCQMNENDSETMAGLLMKEGFTPASAPEEADVVIINTCAVRRKSEEKAYSELGQM 63

Query: 341 IELGQSRGIHVVVAGCVPQGAPKSGYLHGLS-IVGVQQIDRIVEVVEXTLK 490
           +++ + R + V VAGCV +   +     G   ++G + + ++ E V+  L+
Sbjct: 64  LKIKRKRKLVVGVAGCVAEKEREKLLERGADFVLGTRAVLKVTEAVKRALQ 114


>UniRef50_UPI00004984BC Cluster: RNA modification enzymes,
           MiaB-family; n=2; Entamoeba histolytica HM-1:IMSS|Rep:
           RNA modification enzymes, MiaB-family - Entamoeba
           histolytica HM-1:IMSS
          Length = 414

 Score = 58.8 bits (136), Expect = 6e-08
 Identities = 32/86 (37%), Positives = 47/86 (54%), Gaps = 4/86 (4%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDA----QLWLLNSCTVKSPAEDHFK 334
           TI   T+GC+HN SDSE M   L   GYK+           +  ++NSCTVK+P++    
Sbjct: 9   TIKFLTYGCSHNVSDSEVMQKDLINAGYKIDSSSTPISSKYKAVVINSCTVKNPSQQAID 68

Query: 335 NEIELGQSRGIHVVVAGCVPQGAPKS 412
              +  +   + +V+AGCVPQ  PK+
Sbjct: 69  VVQKKCEEANVPLVIAGCVPQADPKA 94


>UniRef50_A0RW56 Cluster: 2-methylthioadenine synthetase; n=1;
           Cenarchaeum symbiosum|Rep: 2-methylthioadenine
           synthetase - Cenarchaeum symbiosum
          Length = 421

 Score = 58.4 bits (135), Expect = 8e-08
 Identities = 32/109 (29%), Positives = 59/109 (54%), Gaps = 2/109 (1%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           I+++ +GC+ + +DSE ++GLL   G+ L     ++   ++ +C VK    +   + I++
Sbjct: 4   IWIEAYGCSASQADSEMISGLLVNGGHTLAASPEESDAGVIVTCAVKDATANRMVHRIKM 63

Query: 350 GQSRGIHVVVAGCVPQGAP--KSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
              R +  VVAGC+P+  P   +    G +++G   I R V VVE  L+
Sbjct: 64  LGGRPL--VVAGCLPKAEPGTMARISPGAALMGPNSIGRTVPVVEAALR 110


>UniRef50_A1ZC85 Cluster: TRNA-I(6)A37 thiotransferase enzyme MiaB;
           n=16; Bacteria|Rep: TRNA-I(6)A37 thiotransferase enzyme
           MiaB - Microscilla marina ATCC 23134
          Length = 493

 Score = 58.0 bits (134), Expect = 1e-07
 Identities = 32/110 (29%), Positives = 61/110 (55%), Gaps = 5/110 (4%)
 Frame = +2

Query: 95  KKREKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWD 274
           K  +K+  EQ+ K+  E+    T+ +Y++++GC  N SDSE +A +++ +G+  T +  +
Sbjct: 12  KPDDKEANEQV-KISEENNTGKTRKLYIESYGCQMNFSDSEIVASIMSEHGFDTTSEVDN 70

Query: 275 AQLWLLNSCTVKSPAEDHFKNEIE-----LGQSRGIHVVVAGCVPQGAPK 409
           A + LLN+C ++  AE   +N +        +  G+ V V GC+ +   K
Sbjct: 71  ADVVLLNTCAIRDNAEQRVRNRLRNLNHIKNKKPGMVVGVLGCMAERLKK 120


>UniRef50_A0LFB7 Cluster: RNA modification enzyme, MiaB family; n=1;
           Syntrophobacter fumaroxidans MPOB|Rep: RNA modification
           enzyme, MiaB family - Syntrophobacter fumaroxidans
           (strain DSM 10017 / MPOB)
          Length = 456

 Score = 57.2 bits (132), Expect = 2e-07
 Identities = 44/124 (35%), Positives = 62/124 (50%), Gaps = 11/124 (8%)
 Frame = +2

Query: 140 LESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPA 319
           L    P  + +YV+T+GC  N  DS+    LL A GY+ T D  DA +  LN+C+V+  A
Sbjct: 5   LAKTAPAPRYLYVRTFGCQMNEYDSQRALRLLCAVGYRPTSDIADADVIFLNTCSVRDKA 64

Query: 320 EDHFKNEIELGQSR-------GIHVVVAGCVPQ----GAPKSGYLHGLSIVGVQQIDRIV 466
           E   K    LG+ R        + +VVAGCV Q    G  K  + H   +VG + I  I 
Sbjct: 65  EQ--KVYSFLGRLRRLKAHRPWLKIVVAGCVAQQLGDGLLKR-FEHVDLVVGTRGIGSIA 121

Query: 467 EVVE 478
            ++E
Sbjct: 122 SLLE 125


>UniRef50_Q9L699 Cluster: UPF0004 protein PM1001; n=289;
           Proteobacteria|Rep: UPF0004 protein PM1001 - Pasteurella
           multocida
          Length = 474

 Score = 57.2 bits (132), Expect = 2e-07
 Identities = 31/83 (37%), Positives = 52/83 (62%), Gaps = 6/83 (7%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLL-AANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKN 337
           TQ +++KTWGC  N  DS  MA LL + +G +LTE   +A + LLN+C+++  A++   +
Sbjct: 2   TQKLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVFH 61

Query: 338 EI----ELGQSR-GIHVVVAGCV 391
           ++    EL + + G+ + V GCV
Sbjct: 62  QLGRWKELKKHKPGLVIGVGGCV 84


>UniRef50_Q895H1 Cluster: MiaB protein; n=11; Bacteria|Rep: MiaB
           protein - Clostridium tetani
          Length = 453

 Score = 56.4 bits (130), Expect = 3e-07
 Identities = 31/84 (36%), Positives = 45/84 (53%), Gaps = 7/84 (8%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           T +++TWGC  N  DSE ++G+L   GYK  EDK  A + + N+C V+  AE   K    
Sbjct: 18  TFFIETWGCQMNEEDSEKLSGMLKNIGYKNAEDKNQADIIIFNTCCVRENAE--LKVYGN 75

Query: 347 LGQSRGIH-------VVVAGCVPQ 397
           LG  +G+        + V GC+ Q
Sbjct: 76  LGALKGLKSKNPNLIIAVCGCMMQ 99


>UniRef50_A4LYJ3 Cluster: TRNA-i(6)A37 thiotransferase enzyme MiaB;
           n=7; Desulfuromonadales|Rep: TRNA-i(6)A37
           thiotransferase enzyme MiaB - Geobacter bemidjiensis Bem
          Length = 441

 Score = 56.4 bits (130), Expect = 3e-07
 Identities = 30/81 (37%), Positives = 47/81 (58%), Gaps = 5/81 (6%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y++T+GC  N SDSE +  L+   GY+ T+D  DA L LLN+C++++ AE      +  
Sbjct: 7   LYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRVYGHLGK 66

Query: 350 GQS-----RGIHVVVAGCVPQ 397
            +S      G+ + V GCV Q
Sbjct: 67  FKSIKKTKPGLIIGVGGCVAQ 87


>UniRef50_Q8TWF4 Cluster: 2-methylthioadenine synthetase; n=1;
           Methanopyrus kandleri|Rep: 2-methylthioadenine
           synthetase - Methanopyrus kandleri
          Length = 423

 Score = 56.4 bits (130), Expect = 3e-07
 Identities = 31/109 (28%), Positives = 55/109 (50%), Gaps = 2/109 (1%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           + V+ +GCA N+ D   +  LL   G+++ ED  +A + +L +C V+   +    N +  
Sbjct: 4   VAVEVYGCAANHDDGRLVRELLRREGFEVVEDAENADVAVLLTCIVRDSVDARMVNRMR- 62

Query: 350 GQSRGIHVVVAGCVPQGAPKSG--YLHGLSIVGVQQIDRIVEVVEXTLK 490
            +   +  VVAGC P+  P+         ++VG + +DRI E V   L+
Sbjct: 63  -ELERVPTVVAGCFPEAYPERARKLRPDAALVGPRHLDRIPEAVRAVLR 110


>UniRef50_A7HAH8 Cluster: RNA modification enzyme, MiaB family; n=4;
           Cystobacterineae|Rep: RNA modification enzyme, MiaB
           family - Anaeromyxobacter sp. Fw109-5
          Length = 460

 Score = 56.0 bits (129), Expect = 4e-07
 Identities = 31/80 (38%), Positives = 43/80 (53%), Gaps = 4/80 (5%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-- 343
           +YV T+GC  N SDS+ M  LL  + +   E   DA L LLN+C V+  AE    + +  
Sbjct: 25  VYVHTFGCQMNASDSDRMIELLGRHAFARAETPDDADLILLNTCAVREKAEQKLLSALGR 84

Query: 344 --ELGQSRGIHVVVAGCVPQ 397
             E+   RG  + V+GCV Q
Sbjct: 85  YREVKARRGALIAVSGCVAQ 104


>UniRef50_O26914 Cluster: UPF0004 protein MTH_826; n=3;
           Methanobacteriaceae|Rep: UPF0004 protein MTH_826 -
           Methanobacterium thermoautotrophicum
          Length = 424

 Score = 56.0 bits (129), Expect = 4e-07
 Identities = 39/106 (36%), Positives = 57/106 (53%), Gaps = 4/106 (3%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y++T+GC  N +DSE MAG+L   G  LT    DA + ++N+C VK P E    N I+ 
Sbjct: 6   VYIETFGCTFNQADSEIMAGVLREEGAVLTGID-DADVIIINTCYVKHPTEHKVINRIKK 64

Query: 350 GQSRGIH--VVVAGCVPQGAP-KSGYLHG-LSIVGVQQIDRIVEVV 475
            Q       +VVAGC+ +  P K   + G  S +G  Q+ R  + V
Sbjct: 65  IQETYPEKGLVVAGCMVEIDPSKLEAISGDASWLGPHQLRRAPQAV 110


>UniRef50_Q8RA72 Cluster: 2-methylthioadenine synthetase; n=9;
           Clostridia|Rep: 2-methylthioadenine synthetase -
           Thermoanaerobacter tengcongensis
          Length = 471

 Score = 55.6 bits (128), Expect = 6e-07
 Identities = 31/78 (39%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
 Frame = +2

Query: 92  SKKREKKDPEQIEKVILESVVPGTQTIY-VKTWGCAHNNSDSEYMAGLLAANGYKLTEDK 268
           S++  KK  E +E++  E+   G +  Y ++T+GC  N  DSE +AG+L   GYK TED 
Sbjct: 8   SEEELKKQREIMEEIAWEN--RGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYKYTEDL 65

Query: 269 WDAQLWLLNSCTVKSPAE 322
             A + L N+C V+  AE
Sbjct: 66  EKADVLLFNTCAVREHAE 83


>UniRef50_Q11BD9 Cluster: RNA modification enzyme, MiaB family;
           n=78; Proteobacteria|Rep: RNA modification enzyme, MiaB
           family - Mesorhizobium sp. (strain BNC1)
          Length = 475

 Score = 55.6 bits (128), Expect = 6e-07
 Identities = 34/85 (40%), Positives = 46/85 (54%), Gaps = 9/85 (10%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-- 343
           ++VKT+GC  N  DS+ MA  LAA GY+ T+   DA L LLN+C ++  A +   +E+  
Sbjct: 27  VFVKTYGCQMNVYDSQRMADALAAEGYRATDVIEDADLVLLNTCHIREKAAEKVYSELGR 86

Query: 344 -------ELGQSRGIHVVVAGCVPQ 397
                     Q R   V VAGCV Q
Sbjct: 87  IRVLKEERAKQGRETVVGVAGCVAQ 111


>UniRef50_Q58277 Cluster: UPF0004 protein MJ0867; n=2;
           Methanococcales|Rep: UPF0004 protein MJ0867 -
           Methanococcus jannaschii
          Length = 427

 Score = 55.6 bits (128), Expect = 6e-07
 Identities = 26/87 (29%), Positives = 49/87 (56%), Gaps = 2/87 (2%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +YV+ +GC  N +D+E +   L  +G+++  +  +A + ++N+C V+   E+     I  
Sbjct: 14  VYVEGYGCVLNTADTEIIKNSLKKHGFEVVNNLEEADIAIINTCVVRLETENRMIYRINE 73

Query: 350 GQSRGIHVVVAGCVPQGAPK--SGYLH 424
            ++ G  VVVAGC+P+       G+LH
Sbjct: 74  LKNLGKEVVVAGCLPKALKNKVKGFLH 100


>UniRef50_Q4HEV7 Cluster: MiaB-like tRNA modifying enzyme; n=19;
           Campylobacterales|Rep: MiaB-like tRNA modifying enzyme -
           Campylobacter coli RM2228
          Length = 418

 Score = 55.2 bits (127), Expect = 7e-07
 Identities = 30/104 (28%), Positives = 54/104 (51%), Gaps = 2/104 (1%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           + ++ KT+GC  N  D+E +   +    Y++  D+  AQ+ ++NSCTV + A+   K+ I
Sbjct: 3   EKVFFKTFGCRTNIYDTELLKSYV--KDYEIVNDEEKAQIIVVNSCTVTNGADSGIKSYI 60

Query: 344 ELGQSRGIHVVVAGC--VPQGAPKSGYLHGLSIVGVQQIDRIVE 469
              Q +G+ V++ GC  V +G           ++G    D+I E
Sbjct: 61  NSMQKKGVRVILTGCGAVSKGKELLDKKQVFGVLGASNKDKINE 104


>UniRef50_Q2FSK8 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Methanospirillum hungatei JF-1|Rep: MiaB-like tRNA
           modifying enzyme - Methanospirillum hungatei (strain
           JF-1 / DSM 864)
          Length = 428

 Score = 55.2 bits (127), Expect = 7e-07
 Identities = 32/112 (28%), Positives = 61/112 (54%)
 Frame = +2

Query: 77  NVSVRSKKREKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKL 256
           N+S ++ ++++ D    EK  ++++    + I ++T+GCA+N  DS+ +A +L A+G  +
Sbjct: 2   NISDQAPEKKRNDLFLPEKEWVKAL--SGRPICIRTFGCAYNVGDSDLLASVLTASGSVI 59

Query: 257 TEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQGAPKS 412
             D   A++ ++N+C V +  E     EI         V V GC+P   P+S
Sbjct: 60  VSDPELAEVMIINTCIVIASTERKMLKEISSYPDH--EVYVTGCLPLALPES 109


>UniRef50_Q64CL1 Cluster: Putative uncharacterized protein; n=1;
           uncultured archaeon GZfos21B5|Rep: Putative
           uncharacterized protein - uncultured archaeon GZfos21B5
          Length = 430

 Score = 55.2 bits (127), Expect = 7e-07
 Identities = 27/90 (30%), Positives = 49/90 (54%)
 Frame = +2

Query: 140 LESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPA 319
           L  +  GT  ++++T+GC  N  D+  M  +L   G+++ E+  +A + ++N+CTV    
Sbjct: 3   LTELSEGTAKVFIETFGCTANTGDTMEMRAILRNAGHEIVEES-EADIVIVNTCTVTKRT 61

Query: 320 EDHFKNEIELGQSRGIHVVVAGCVPQGAPK 409
           E +    +   + RG  VVVAGC+    P+
Sbjct: 62  ELNVIKRLNELKERGKAVVVAGCMAAAQPE 91


>UniRef50_Q7QYP6 Cluster: GLP_393_20381_21958; n=1; Giardia lamblia
           ATCC 50803|Rep: GLP_393_20381_21958 - Giardia lamblia
           ATCC 50803
          Length = 525

 Score = 54.0 bits (124), Expect = 2e-06
 Identities = 30/85 (35%), Positives = 44/85 (51%), Gaps = 9/85 (10%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLT---------EDKWDAQLWLLNSCTVKSPAE 322
           + + T GC HN ++S+ +A  L   G  +T         E   D  +  +NSCTVK+P+E
Sbjct: 26  VMMVTMGCGHNAAESDIIASALQTAGAVITHSNGKYITPESARDVDVLYINSCTVKNPSE 85

Query: 323 DHFKNEIELGQSRGIHVVVAGCVPQ 397
           D     ++ G   G  VV+ GCVPQ
Sbjct: 86  DKAFVHVQKGLEVGTVVVLGGCVPQ 110


>UniRef50_Q74MF6 Cluster: NEQ008; n=1; Nanoarchaeum equitans|Rep:
           NEQ008 - Nanoarchaeum equitans
          Length = 413

 Score = 54.0 bits (124), Expect = 2e-06
 Identities = 34/108 (31%), Positives = 51/108 (47%), Gaps = 1/108 (0%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y +++GC  N  D+ YM   +      L E    A + ++NSC VK P E      I  
Sbjct: 3   VYFESYGCTLNKRDTLYMQAQIENTTNNLEE----ADVVVINSCIVKQPTETKILYRINQ 58

Query: 350 GQSRGIHVVVAGC-VPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
            +  G  +V+ GC V +   K   L  +S+V +   DRI E +E T K
Sbjct: 59  LKKMGKKIVLTGCMVSEPYLKYKELQDISLVNIYNQDRIKEAIERTYK 106


>UniRef50_A3DNI7 Cluster: RNA modification enzyme, MiaB family; n=1;
           Staphylothermus marinus F1|Rep: RNA modification enzyme,
           MiaB family - Staphylothermus marinus (strain ATCC 43588
           / DSM 3639 / F1)
          Length = 429

 Score = 54.0 bits (124), Expect = 2e-06
 Identities = 25/83 (30%), Positives = 44/83 (53%), Gaps = 4/83 (4%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE----DHFKN 337
           IY++T+GCA N  D   M  +L + G+KL E+  +A   ++N+CTV+   E       K 
Sbjct: 5   IYIETYGCALNRGDEYIMKTVLVSRGHKLVEEITEADTIIINTCTVRYDTELKMIKRIKE 64

Query: 338 EIELGQSRGIHVVVAGCVPQGAP 406
              +   +   +++AGC+ +  P
Sbjct: 65  LYRIASEQNKKLIIAGCMAKAQP 87


>UniRef50_Q2RZF8 Cluster: TRNA-i(6)A37 thiotransferase enzyme MiaB;
           n=12; cellular organisms|Rep: TRNA-i(6)A37
           thiotransferase enzyme MiaB - Salinibacter ruber (strain
           DSM 13855)
          Length = 572

 Score = 53.6 bits (123), Expect = 2e-06
 Identities = 27/92 (29%), Positives = 52/92 (56%), Gaps = 1/92 (1%)
 Frame = +2

Query: 86  VRSKKREKKDPEQIEKVILE-SVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTE 262
           VR ++ + +  E +++V        G + +Y++T+GC  N +DS  +A +L  +GY LT 
Sbjct: 84  VRQREADGEVDEDLDRVKHGYDATAGDKQVYIETYGCQMNVNDSGIVASVLEESGYGLTR 143

Query: 263 DKWDAQLWLLNSCTVKSPAEDHFKNEIELGQS 358
           D+  A + LLN+C ++  AE   +  + + +S
Sbjct: 144 DQAAADVVLLNTCAIRENAERKIRARLSMLRS 175


>UniRef50_A7CVG2 Cluster: RNA modification enzyme, MiaB family
           precursor; n=1; Opitutaceae bacterium TAV2|Rep: RNA
           modification enzyme, MiaB family precursor - Opitutaceae
           bacterium TAV2
          Length = 562

 Score = 53.6 bits (123), Expect = 2e-06
 Identities = 21/51 (41%), Positives = 33/51 (64%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           +Y+KT+GC  N  DS  +A +L A GY++   + D  + LLN+C+V+  AE
Sbjct: 72  VYIKTYGCQMNERDSNAVAAMLRARGYRIVNTEDDCDIMLLNTCSVRDAAE 122


>UniRef50_A3EV78 Cluster: 2-methylthioadenine synthetase; n=1;
           Leptospirillum sp. Group II UBA|Rep: 2-methylthioadenine
           synthetase - Leptospirillum sp. Group II UBA
          Length = 468

 Score = 53.6 bits (123), Expect = 2e-06
 Identities = 30/83 (36%), Positives = 46/83 (55%), Gaps = 5/83 (6%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +T Y+KT+GC  N  DSE MAGLL A G     +   A + L+N+CT++  A+    +++
Sbjct: 28  KTFYIKTFGCQMNVHDSERMAGLLTAEGGNPVSEPAAADIILVNTCTIRDKADQKALSDL 87

Query: 344 -ELGQSR----GIHVVVAGCVPQ 397
             + Q R    G  + V GC+ Q
Sbjct: 88  GRIRQVRKEGPGTILAVTGCMAQ 110


>UniRef50_O31778 Cluster: UPF0004 protein ymcB; n=55;
           Firmicutes|Rep: UPF0004 protein ymcB - Bacillus subtilis
          Length = 509

 Score = 53.6 bits (123), Expect = 2e-06
 Identities = 23/57 (40%), Positives = 35/57 (61%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           Y++T+GC  N  D+E MAG+  A GY+ T    DA + LLN+C ++  AE+    E+
Sbjct: 69  YIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGEL 125


>UniRef50_A0B642 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Methanosaeta thermophila PT|Rep: MiaB-like tRNA
           modifying enzyme - Methanosaeta thermophila (strain DSM
           6194 / PT) (Methanothrixthermophila (strain DSM 6194 /
           PT))
          Length = 411

 Score = 53.2 bits (122), Expect = 3e-06
 Identities = 31/103 (30%), Positives = 57/103 (55%), Gaps = 3/103 (2%)
 Frame = +2

Query: 176 VKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ 355
           ++T+GC  N  +S  + G L A+G++   D   +++ +LN+C V S  E +    I  G+
Sbjct: 5   IETYGCTSNTGNSMELRGALIAHGHQ-ESDLDGSEVVILNTCAVTSRTERNMLRRI--GE 61

Query: 356 SRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQ---IDRIVEVV 475
            +G  ++VAGC+P   P+   +  +  VGV     IDR+++ +
Sbjct: 62  LKGRRLIVAGCLPAAIPE--LIESVECVGVLNRWGIDRVLDAL 102


>UniRef50_Q74B44 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=4; Deltaproteobacteria|Rep: TRNA-i(6)A37 modification
           enzyme MiaB - Geobacter sulfurreducens
          Length = 446

 Score = 52.8 bits (121), Expect = 4e-06
 Identities = 30/81 (37%), Positives = 45/81 (55%), Gaps = 5/81 (6%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-- 343
           +YV+T+GC  N +DSE +A LL   GY  T+D   A L +LN+C+V++ AE      +  
Sbjct: 7   LYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKVYGHLGR 66

Query: 344 ---ELGQSRGIHVVVAGCVPQ 397
                 + +G  + V GCV Q
Sbjct: 67  FKGVRSRKKGFLLGVGGCVAQ 87


>UniRef50_UPI00015B4592 Cluster: PREDICTED: similar to radical sam
           proteins; n=1; Nasonia vitripennis|Rep: PREDICTED:
           similar to radical sam proteins - Nasonia vitripennis
          Length = 660

 Score = 52.4 bits (120), Expect = 5e-06
 Identities = 27/86 (31%), Positives = 51/86 (59%), Gaps = 1/86 (1%)
 Frame = +2

Query: 92  SKKREKKDPEQIEKVILESVVPGT-QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDK 268
           SK   + + E  EK+   S + G  Q +Y++ +GC  N +D+E ++ +L  + YK+T+D 
Sbjct: 139 SKPSHRSEVES-EKIPYLSPLDGDLQKVYLEVYGCQMNVNDTEVVSAILKKHNYKITKDI 197

Query: 269 WDAQLWLLNSCTVKSPAEDHFKNEIE 346
            DA + LL +C ++  AE+   N+++
Sbjct: 198 MDANVILLVTCAIRENAENKVWNKLK 223


>UniRef50_Q2GCU4 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=11; Rickettsiales|Rep: TRNA-i(6)A37 modification
           enzyme MiaB - Neorickettsia sennetsu (strain Miyayama)
          Length = 471

 Score = 52.4 bits (120), Expect = 5e-06
 Identities = 38/127 (29%), Positives = 65/127 (51%), Gaps = 12/127 (9%)
 Frame = +2

Query: 131 KVILESVVPGTQTI---YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC 301
           KV +E +     ++   ++KT+GC  N  DSE +  +++  G+ L+E   DA L +LN+C
Sbjct: 15  KVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLSERAEDADLIILNTC 74

Query: 302 TVKSPAEDHFKNE---IELGQSR---GIHVVVAGCVPQGAPKSGYLHGLS---IVGVQQI 454
            ++  A +   +E   I L Q +    I +VVAGCV Q   +       +   +VG Q I
Sbjct: 75  NIREKAAEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMRRAENVDVVVGPQSI 134

Query: 455 DRIVEVV 475
             + E++
Sbjct: 135 HSLPELI 141


>UniRef50_Q8TRM2 Cluster: 2-methylthioadenine synthase; n=4;
           Methanosarcinaceae|Rep: 2-methylthioadenine synthase -
           Methanosarcina acetivorans
          Length = 435

 Score = 52.4 bits (120), Expect = 5e-06
 Identities = 30/107 (28%), Positives = 58/107 (54%), Gaps = 5/107 (4%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDK--WDAQLWLLNSCTVKSPAEDHFKNEI 343
           +Y++++GC+ + + +E M   +   G++L       +A++++ NSCTVK   E     +I
Sbjct: 3   VYLESFGCSASLASAEIMKASVERLGHELLNPAAAGEAEVYICNSCTVKYTTEQKILYKI 62

Query: 344 ELGQSRGIHVVVAGCVPQGAPKSGYLHG---LSIVGVQQIDRIVEVV 475
                +G+ V+V+GC+P+       LH      I+GV  I R+ E++
Sbjct: 63  RSMGEKGVQVIVSGCMPE-VQLEEILHANPEAHILGVNAISRLGELL 108


>UniRef50_Q55803 Cluster: UPF0004 protein slr0082; n=36;
           Cyanobacteria|Rep: UPF0004 protein slr0082 -
           Synechocystis sp. (strain PCC 6803)
          Length = 443

 Score = 52.4 bits (120), Expect = 5e-06
 Identities = 29/112 (25%), Positives = 59/112 (52%), Gaps = 4/112 (3%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKN 337
           T TI +   GC  N  DSE+M GLL   GY++  ++  A   ++N+C+ ++   ++  + 
Sbjct: 4   TPTIAINHLGCEKNRIDSEHMLGLLVEAGYQVDANEELADYVIVNTCSFIQDARQESVRT 63

Query: 338 EIELGQSRGIHVVVAGCVPQGAPK---SGYLHGLSIVGVQQIDRIVEVVEXT 484
            +EL +++   +V++GC+ Q   +         +++VG      IV+++  T
Sbjct: 64  LVELAEAKK-KIVISGCLAQHFQEQLLEEIPEAVAVVGTGDYQNIVDIIRRT 114


>UniRef50_A6DI62 Cluster: Putative uncharacterized protein; n=1;
           Lentisphaera araneosa HTCC2155|Rep: Putative
           uncharacterized protein - Lentisphaera araneosa HTCC2155
          Length = 469

 Score = 52.0 bits (119), Expect = 7e-06
 Identities = 31/91 (34%), Positives = 48/91 (52%), Gaps = 5/91 (5%)
 Frame = +2

Query: 152 VPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHF 331
           +P T  I V + GCA N  D+E M G +A +G  +T D  DA ++++N+C+    A    
Sbjct: 1   MPKTAKICVSSLGCAKNLVDTEVMLGSMAKSGVVITGDLNDADIFVVNTCSFIEGARQES 60

Query: 332 KNEIE-----LGQSRGIHVVVAGCVPQGAPK 409
              I        + +   VVVAGC+PQ +P+
Sbjct: 61  NAAIMDAITWKKKRKSRKVVVAGCLPQRSPE 91


>UniRef50_A0D7J9 Cluster: Chromosome undetermined scaffold_40, whole
           genome shotgun sequence; n=1; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_40,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 504

 Score = 52.0 bits (119), Expect = 7e-06
 Identities = 22/89 (24%), Positives = 51/89 (57%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELG 352
           +++T+GC  N +DS+ +  +L++ GY  T D  +A +  LN+C++++ AE      +   
Sbjct: 48  FIETYGCQMNANDSQIVQSILSSEGYSNTNDISEADIIFLNTCSIRANAEKKVFQRMSEL 107

Query: 353 QSRGIHVVVAGCVPQGAPKSGYLHGLSIV 439
           +S+   + + GC+ +   +  ++ G +I+
Sbjct: 108 KSQNKVLGILGCMAERLKEQLFVQGANII 136


>UniRef50_Q8MXQ7 Cluster: CDKAL1-like protein; n=1; Caenorhabditis
           elegans|Rep: CDKAL1-like protein - Caenorhabditis
           elegans
          Length = 425

 Score = 52.0 bits (119), Expect = 7e-06
 Identities = 25/38 (65%), Positives = 29/38 (76%)
 Frame = +2

Query: 377 VAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXTLK 490
           +AGCV Q AP   +L  +SIVGV+QIDRIVEVV  TLK
Sbjct: 1   MAGCVSQAAPSEPWLQNVSIVGVKQIDRIVEVVGETLK 38


>UniRef50_A6DMH4 Cluster: Putative uncharacterized protein; n=1;
           Lentisphaera araneosa HTCC2155|Rep: Putative
           uncharacterized protein - Lentisphaera araneosa HTCC2155
          Length = 452

 Score = 51.6 bits (118), Expect = 9e-06
 Identities = 33/113 (29%), Positives = 59/113 (52%), Gaps = 8/113 (7%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE- 340
           + + +KT+GC  N+ DSE +   L  +GY++T ++ DA + +LN+C+V+  AE     + 
Sbjct: 4   EKVLIKTYGCQMNDRDSEAVEMDLLKSGYEITTEEKDADVIILNTCSVRDQAERKALGKV 63

Query: 341 ---IELGQSR-GIHVVVAGCVPQGAPK---SGYLHGLSIVGVQQIDRIVEVVE 478
              I+L +    + V V GC+ Q           H   + G  Q+ +I E++E
Sbjct: 64  GSLIKLRRKNPKLQVGVIGCMAQSRADDIVEKNAHVNFVAGTDQLHKIPELIE 116


>UniRef50_A4SAH0 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
           Predicted protein - Ostreococcus lucimarinus CCE9901
          Length = 579

 Score = 51.6 bits (118), Expect = 9e-06
 Identities = 22/55 (40%), Positives = 35/55 (63%)
 Frame = +2

Query: 158 GTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           G + +YV+T+GC  N +DSE M  +L   GY  T++  DA + L+N+C ++  AE
Sbjct: 65  GRRAVYVETYGCQMNVNDSEVMMAVLEGAGYDETKEVNDADVILINTCAIRDKAE 119


>UniRef50_Q7MAW4 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=3; Porphyromonadaceae|Rep: TRNA-i(6)A37 modification
           enzyme MiaB - Porphyromonas gingivalis (Bacteroides
           gingivalis)
          Length = 463

 Score = 51.2 bits (117), Expect = 1e-05
 Identities = 21/58 (36%), Positives = 38/58 (65%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +Y++T+GC  N +DSE +A ++  +GY LT++  +A   L+N+C+V+  AE    N +
Sbjct: 20  LYIETYGCQMNVADSEVVASVMQMDGYNLTDNVDEADTILVNTCSVRDNAEQKVLNRL 77


>UniRef50_Q7ULM9 Cluster: Probable MiaB protein-putative
           tRNA-thiotransferase; n=2; Planctomycetaceae|Rep:
           Probable MiaB protein-putative tRNA-thiotransferase -
           Rhodopirellula baltica
          Length = 479

 Score = 50.8 bits (116), Expect = 2e-05
 Identities = 27/84 (32%), Positives = 48/84 (57%), Gaps = 5/84 (5%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           T+T+Y+KT GC  N  DSE +   L  +GY + +   +A L L N+C+++  AE+   + 
Sbjct: 5   TKTVYIKTVGCQMNVLDSEMVIADLKRHGYTVVDTPGEADLLLYNTCSIREQAEEKTYSA 64

Query: 341 I-ELGQSRGIH----VVVAGCVPQ 397
           + +L +++  H    + V GC+ Q
Sbjct: 65  LGKLKETKARHPEKTIGVMGCMAQ 88


>UniRef50_Q2LQ68 Cluster: TRNA 2-methylthioadenine synthetase-like
           protein; n=1; Syntrophus aciditrophicus SB|Rep: TRNA
           2-methylthioadenine synthetase-like protein - Syntrophus
           aciditrophicus (strain SB)
          Length = 453

 Score = 50.8 bits (116), Expect = 2e-05
 Identities = 33/111 (29%), Positives = 58/111 (52%), Gaps = 10/111 (9%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI- 343
           ++++ + GC  N  DSE MA LL   G ++     +A + LLN+C    PA +   +EI 
Sbjct: 5   SVHIVSLGCPKNLIDSEVMAALLEQAGCRIVSGPEEADILLLNTCAFILPAREESIDEIF 64

Query: 344 ------ELGQSRGIHVVVAGCVPQ--GAPKSGYLHGLSI-VGVQQIDRIVE 469
                 + G+ R  H++V GC+PQ  GA  +  L  + + +G+ ++  I +
Sbjct: 65  RLAEWKKAGKCR--HLIVTGCLPQRYGAELAAELPEVDLFLGISEVPNIAD 113


>UniRef50_A4FZ90 Cluster: MiaB-like tRNA modifying enzyme; n=4;
           Methanococcus|Rep: MiaB-like tRNA modifying enzyme -
           Methanococcus maripaludis
          Length = 425

 Score = 50.8 bits (116), Expect = 2e-05
 Identities = 25/81 (30%), Positives = 46/81 (56%), Gaps = 1/81 (1%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAA-NGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           IY++ +GC  N +D+E +   +     ++LT++  D+ + ++N+C V+   E    + IE
Sbjct: 3   IYIEGYGCTLNTADTEIIKNSVNEFEDFELTDNVDDSDIIVINTCIVRQETEHRMISRIE 62

Query: 347 LGQSRGIHVVVAGCVPQGAPK 409
             +S    VVVAGC+ +  PK
Sbjct: 63  YFKSLDKKVVVAGCMAKALPK 83


>UniRef50_Q74A23 Cluster: MiaB-like tRNA modifying enzyme; n=3;
           Deltaproteobacteria|Rep: MiaB-like tRNA modifying enzyme
           - Geobacter sulfurreducens
          Length = 434

 Score = 50.4 bits (115), Expect = 2e-05
 Identities = 30/95 (31%), Positives = 48/95 (50%), Gaps = 3/95 (3%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           Q + + T GC  N  +S  M   L   G++L   + +A ++++N+CTV +  +   +  I
Sbjct: 2   QRVAITTLGCKINQFESAAMTESLGREGFRLVPFEDEADIYVINTCTVTARTDAESRRLI 61

Query: 344 ELGQSR--GIHVVVAGCVPQGAPKS-GYLHGLSIV 439
                R     VVV GC  Q AP + G L G+S+V
Sbjct: 62  RRAMRRNPAARVVVTGCYAQVAPDAVGELPGVSLV 96


>UniRef50_O66638 Cluster: UPF0004 protein aq_284; n=2; Aquifex
           aeolicus|Rep: UPF0004 protein aq_284 - Aquifex aeolicus
          Length = 440

 Score = 50.4 bits (115), Expect = 2e-05
 Identities = 29/86 (33%), Positives = 49/86 (56%), Gaps = 7/86 (8%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           ++  ++KT+GC  N +DSE + GLL   GY+ T++  +A L +LN+CT++   +   K  
Sbjct: 2   SKKFFIKTFGCQMNFNDSERIRGLLKTIGYEQTDNWEEADLIILNTCTIREKPDQ--KVL 59

Query: 341 IELGQSRGIH-------VVVAGCVPQ 397
             LG+ + I        + VAGC+ Q
Sbjct: 60  SHLGEYKKIKEKNPKALIAVAGCLAQ 85


>UniRef50_Q8EUX4 Cluster: Putative uncharacterized protein MYPE7940;
           n=1; Mycoplasma penetrans|Rep: Putative uncharacterized
           protein MYPE7940 - Mycoplasma penetrans
          Length = 491

 Score = 50.0 bits (114), Expect = 3e-05
 Identities = 23/53 (43%), Positives = 33/53 (62%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           +T ++KT+GC  N  D+E M G+L   GY+  ED   + L LLN+C V+  AE
Sbjct: 54  KTYHIKTFGCQSNLRDTEVMMGMLELIGYEYNEDVNTSDLVLLNTCAVREHAE 106


>UniRef50_P73127 Cluster: UPF0004 protein sll0996; n=37;
           Cyanobacteria|Rep: UPF0004 protein sll0996 -
           Synechocystis sp. (strain PCC 6803)
          Length = 451

 Score = 50.0 bits (114), Expect = 3e-05
 Identities = 36/110 (32%), Positives = 57/110 (51%), Gaps = 8/110 (7%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELG 352
           ++ T+GC  N +DSE MAG+L   G   T+D   A L L N+C+++  AE    + +   
Sbjct: 9   HIITFGCQMNKADSERMAGILENLGMTYTDDPNQADLVLYNTCSIRDNAEQKVYSYLGRQ 68

Query: 353 QSR-----GIHVVVAGCVPQ--GAPKSGYLHGLSIV-GVQQIDRIVEVVE 478
             R      + +VVAGCV Q  G      +  L +V G Q  +R+ +++E
Sbjct: 69  AKRKQVEPELTLVVAGCVAQQEGEQLLRRVPELDLVMGPQHANRLDQLLE 118


>UniRef50_Q9WZT7 Cluster: UPF0004 protein TM_0830; n=2;
           Thermotoga|Rep: UPF0004 protein TM_0830 - Thermotoga
           maritima
          Length = 434

 Score = 50.0 bits (114), Expect = 3e-05
 Identities = 30/105 (28%), Positives = 56/105 (53%), Gaps = 3/105 (2%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +T+ ++T+GC  N  +SEYMA  L   GY +  D  +A  +++NSC V    E   K  I
Sbjct: 2   KTVRIETFGCKVNQYESEYMAEQLEKAGYVVLPD-GNAAYYIVNSCAVTKEVEKKVKRLI 60

Query: 344 E--LGQSRGIHVVVAGCVPQGAP-KSGYLHGLSIVGVQQIDRIVE 469
           +    +++   +++ GC  Q +P ++  L    ++G+ +   IV+
Sbjct: 61  KSIRNRNKNAKIILTGCFAQLSPDEAKNLPVDMVLGIDEKKHIVD 105


>UniRef50_Q6MAB7 Cluster: Probable 2-methylthioadenine synthetase;
           n=1; Candidatus Protochlamydia amoebophila UWE25|Rep:
           Probable 2-methylthioadenine synthetase - Protochlamydia
           amoebophila (strain UWE25)
          Length = 450

 Score = 49.6 bits (113), Expect = 4e-05
 Identities = 29/85 (34%), Positives = 47/85 (55%), Gaps = 4/85 (4%)
 Frame = +2

Query: 149 VVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDH 328
           ++   +  +VKT+GC  N  DSE M G L   G   + D+ DA L + N+C+++  AE  
Sbjct: 12  IMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFNTCSIRDLAERK 71

Query: 329 FKNEI-ELG---QSRGIHVVVAGCV 391
              ++ +LG   QS+ I + V GC+
Sbjct: 72  VMGKLGKLGLTKQSQAI-IGVTGCM 95


>UniRef50_Q0AWM7 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Syntrophomonas wolfei subsp. wolfei str. Goettingen|Rep:
           MiaB-like tRNA modifying enzyme - Syntrophomonas wolfei
           subsp. wolfei (strain Goettingen)
          Length = 456

 Score = 49.6 bits (113), Expect = 4e-05
 Identities = 32/103 (31%), Positives = 53/103 (51%), Gaps = 4/103 (3%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSR 361
           T GC  N  ++E +       GY+L +    A L+++N+CTV   ++   +  +     R
Sbjct: 8   TLGCKVNQVETEQLKEKFIQRGYQLVDFNESADLYIVNTCTVTHSSDRKSRAMLRRAARR 67

Query: 362 --GIHVVVAGCVPQ-GAPKSGYLHGLS-IVGVQQIDRIVEVVE 478
             G  VV  GC+ Q  A +   + GL+ IVG QQ + I+E++E
Sbjct: 68  NPGAMVVATGCLAQVDAAQLAAIPGLNLIVGSQQKEAILELIE 110


>UniRef50_A7H6G8 Cluster: MiaB-like tRNA modifying enzyme YliG;
           n=10; Deltaproteobacteria|Rep: MiaB-like tRNA modifying
           enzyme YliG - Anaeromyxobacter sp. Fw109-5
          Length = 470

 Score = 49.6 bits (113), Expect = 4e-05
 Identities = 29/84 (34%), Positives = 43/84 (51%), Gaps = 5/84 (5%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKN 337
           T  +Y+ T GC  N  DSE M G L   GY+L  D   A + ++N+C  ++S  E+    
Sbjct: 3   TTRVYLHTLGCPKNRVDSEVMLGTLTGAGYRLERDPAQADVIVVNTCGFIESAKEESVDA 62

Query: 338 EIELG----QSRGIHVVVAGCVPQ 397
            +EL     + R   +VV GC+ Q
Sbjct: 63  IVELAGMKQEGRCKKLVVTGCLVQ 86


>UniRef50_Q49842 Cluster: UPF0004 protein ML0989; n=71;
           Actinobacteria (class)|Rep: UPF0004 protein ML0989 -
           Mycobacterium leprae
          Length = 517

 Score = 49.6 bits (113), Expect = 4e-05
 Identities = 30/93 (32%), Positives = 49/93 (52%), Gaps = 8/93 (8%)
 Frame = +2

Query: 143 ESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDK---WDAQLWLLNSCTVKS 313
           ++    T+T  V+T+GC  N  DSE +AGLL A GY+   D+    DA + + N+C V+ 
Sbjct: 11  DAATGSTRTYQVRTYGCQMNVHDSERLAGLLEAAGYQRAADEADVGDADVVVFNTCAVRE 70

Query: 314 PAEDH-FKNEIELGQSR----GIHVVVAGCVPQ 397
            A++  + N   L   +     + + V GC+ Q
Sbjct: 71  NADNRLYGNLSHLAPRKRNNPDMQIAVGGCLAQ 103


>UniRef50_Q9ZCE8 Cluster: UPF0004 protein RP808; n=15;
           Alphaproteobacteria|Rep: UPF0004 protein RP808 -
           Rickettsia prowazekii
          Length = 445

 Score = 49.6 bits (113), Expect = 4e-05
 Identities = 31/89 (34%), Positives = 52/89 (58%), Gaps = 10/89 (11%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           ++ +Y+KT+GC  N  DS  +  LL   GY+ TED  +A + +LN+C ++  A +   +E
Sbjct: 2   SKKLYIKTYGCQMNVYDSVKIQDLLYPFGYESTEDIKEADIIILNTCHIREKAAEKTYSE 61

Query: 341 I----ELGQSR---GIH---VVVAGCVPQ 397
           +    +L  +R   G++   +VVAGCV Q
Sbjct: 62  LGRIKKLQNTRKQEGLNPAIIVVAGCVAQ 90


>UniRef50_A6PSP0 Cluster: RNA modification enzyme, MiaB family; n=1;
           Victivallis vadensis ATCC BAA-548|Rep: RNA modification
           enzyme, MiaB family - Victivallis vadensis ATCC BAA-548
          Length = 446

 Score = 49.2 bits (112), Expect = 5e-05
 Identities = 20/51 (39%), Positives = 32/51 (62%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           I++KT+GC  N  DSE  AG+L   G+ + + +  A + L N+C+V+  AE
Sbjct: 3   IFIKTYGCQMNERDSEAFAGMLVEAGHTMVDSEEQADVLLFNTCSVREQAE 53


>UniRef50_A5UUG7 Cluster: RNA modification enzyme, MiaB family; n=5;
           Chloroflexi (class)|Rep: RNA modification enzyme, MiaB
           family - Roseiflexus sp. RS-1
          Length = 476

 Score = 49.2 bits (112), Expect = 5e-05
 Identities = 32/97 (32%), Positives = 47/97 (48%), Gaps = 5/97 (5%)
 Frame = +2

Query: 116 PEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLN 295
           PE+          P  +  YV T GC  N SDSE +   L   GY   E   DA   +LN
Sbjct: 10  PEEARATQSRDATPRERRYYVWTVGCQMNVSDSERLEAALQGVGYAPAERPEDASFIVLN 69

Query: 296 SCTVKSPAEDHFKNEI-ELGQSRGIH----VVVAGCV 391
           SC+V++ AE+    ++ E+ + +  H    VV+ GC+
Sbjct: 70  SCSVRASAEERILGKLSEVQRLKRKHPDTKVVLWGCM 106


>UniRef50_A4XKJ7 Cluster: RNA modification enzyme, MiaB family; n=2;
           Clostridiales|Rep: RNA modification enzyme, MiaB family
           - Caldicellulosiruptor saccharolyticus (strain ATCC
           43494 / DSM 8903)
          Length = 434

 Score = 49.2 bits (112), Expect = 5e-05
 Identities = 32/107 (29%), Positives = 57/107 (53%), Gaps = 4/107 (3%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ-- 355
           T GC  N  +++ +A      GY++ +   +A ++++N+CTV + ++   +  I+  +  
Sbjct: 7   TLGCKVNQYETQAIAETFERLGYEIVDFDQEADIYVINTCTVTNVSDRKSRQAIKRAKKT 66

Query: 356 SRGIHVVVAGCVPQGAPKS-GYLHGLS-IVGVQQIDRIVEVVEXTLK 490
           S    VVV GC PQ  P+    + G+  IVG +  ++IVE V   LK
Sbjct: 67  SPDSIVVVMGCYPQVYPQEVQKIEGVDIIVGTRDREKIVEYVTEYLK 113


>UniRef50_P56131 Cluster: UPF0004 protein HP_0269; n=26;
           Epsilonproteobacteria|Rep: UPF0004 protein HP_0269 -
           Helicobacter pylori (Campylobacter pylori)
          Length = 437

 Score = 49.2 bits (112), Expect = 5e-05
 Identities = 23/58 (39%), Positives = 35/58 (60%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +Y++T GCA N+ DSE++   L+   YK T D   A L L+N+C+V+   E    +EI
Sbjct: 3   VYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKTADLILINTCSVREKPERKLFSEI 60


>UniRef50_Q1V1E1 Cluster: TRNA-i(6)A37 modification enzyme; n=2;
           Candidatus Pelagibacter ubique|Rep: TRNA-i(6)A37
           modification enzyme - Candidatus Pelagibacter ubique
           HTCC1002
          Length = 455

 Score = 48.8 bits (111), Expect = 7e-05
 Identities = 27/81 (33%), Positives = 44/81 (54%), Gaps = 5/81 (6%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE- 346
           I++KT+GC  N  DS  +   +   G++ TE   DA  +LLN+C ++  A++   +EI  
Sbjct: 14  IFIKTFGCQMNEYDSNRIFDTVKKIGFEKTEKYEDANCYLLNTCHIRDKAKEKVYHEIGR 73

Query: 347 ----LGQSRGIHVVVAGCVPQ 397
                 + +   V+VAGCV Q
Sbjct: 74  VKKIFREKKKPIVIVAGCVAQ 94


>UniRef50_Q3AU39 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=9; Chlorobiaceae|Rep: TRNA-i(6)A37 modification enzyme
           MiaB - Chlorobium chlorochromatii (strain CaD3)
          Length = 449

 Score = 48.4 bits (110), Expect = 9e-05
 Identities = 27/81 (33%), Positives = 41/81 (50%), Gaps = 6/81 (7%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED---HFKNEI 343
           Y+ T+GC  N +DS  M  +L   GY    ++ DA + LLN+C V+  A +   H    +
Sbjct: 10  YIHTFGCQMNQADSGIMTAILQNEGYVAASNEADAGIVLLNTCAVREHATERVGHLLQHL 69

Query: 344 ELGQSRG---IHVVVAGCVPQ 397
              + R    + V V GC+PQ
Sbjct: 70  HGRKKRSKGRLLVGVTGCIPQ 90


>UniRef50_Q1PZS6 Cluster: Putative uncharacterized protein; n=1;
           Candidatus Kuenenia stuttgartiensis|Rep: Putative
           uncharacterized protein - Candidatus Kuenenia
           stuttgartiensis
          Length = 447

 Score = 48.4 bits (110), Expect = 9e-05
 Identities = 20/54 (37%), Positives = 34/54 (62%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED 325
           +T++ +T+GC  N  D+E   GLL  +GY + +   +A + L N+C+V+  AED
Sbjct: 14  KTVFFETFGCQMNKLDAELSLGLLQEDGYSIVDKVEEADVILYNTCSVRQHAED 67


>UniRef50_Q2RJK1 Cluster: Putative uncharacterized protein; n=1;
           Moorella thermoacetica ATCC 39073|Rep: Putative
           uncharacterized protein - Moorella thermoacetica (strain
           ATCC 39073)
          Length = 432

 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 29/112 (25%), Positives = 55/112 (49%), Gaps = 5/112 (4%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKNEIE 346
           + V T GC  N  +SEYM G+L  N  ++  D   A++ ++N+C+ + +  E+     +E
Sbjct: 4   VAVITLGCPKNQVESEYMLGILEKNHLEVVSDPRQAEVVIINTCSFITAAREEALDTILE 63

Query: 347 LGQSRG-IHVVVAGCVPQGAPKSGYL---HGLSIVGVQQIDRIVEVVEXTLK 490
           L ++     ++VAGC+ Q      +       + +G     R+ E++   LK
Sbjct: 64  LARAANHPRLIVAGCLAQQYASELWQELPEAAAFIGPGATGRLPEIINRVLK 115


>UniRef50_Q028J0 Cluster: MiaB-like tRNA modifying enzyme YliG; n=2;
           Acidobacteria|Rep: MiaB-like tRNA modifying enzyme YliG
           - Solibacter usitatus (strain Ellin6076)
          Length = 465

 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 31/105 (29%), Positives = 55/105 (52%), Gaps = 8/105 (7%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-ELGQ--- 355
           GC  N  DSE M G L A G++LT     A + ++N+C+   PA+    + I E+ +   
Sbjct: 9   GCPKNLVDSEVMMGQLVAKGHELTSHPDQADVLVVNTCSFIDPAKKESVDTILEMAEYKK 68

Query: 356 -SRGIHVVVAGCVPQ---GAPKSGYLHGLSIVGVQQIDRIVEVVE 478
             R   ++VAGC+ +   G  ++      +++G  ++D IV++ E
Sbjct: 69  IGRAKKLIVAGCLVERYRGDIRTEMPEVDALIGTNELDSIVDICE 113


>UniRef50_A5D2R3 Cluster: 2-methylthioadenine synthetase; n=3;
           Clostridiales|Rep: 2-methylthioadenine synthetase -
           Pelotomaculum thermopropionicum SI
          Length = 444

 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 33/109 (30%), Positives = 53/109 (48%), Gaps = 8/109 (7%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKNEIELGQS-- 358
           GC  N  DSE M G+L   GY++T  + +A + ++N+C+ +    E+  +  IEL ++  
Sbjct: 11  GCPKNLVDSEIMLGILKKAGYEITAREKEADVLIVNTCSFINDAKEESIRTIIELARNKI 70

Query: 359 --RGIHVVVAGCVPQGAPKSGYLHGLSI---VGVQQIDRIVEVVEXTLK 490
             R   ++VAGC+ Q  P         I   VG  Q+  I   V   L+
Sbjct: 71  NGRCRAILVAGCLAQRYPAELMAEMPEIDGLVGTGQVPEIARAVRRVLE 119


>UniRef50_A4J5U4 Cluster: MiaB-like tRNA modifying enzyme YliG; n=4;
           Clostridiales|Rep: MiaB-like tRNA modifying enzyme YliG
           - Desulfotomaculum reducens MI-1
          Length = 444

 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 36/110 (32%), Positives = 56/110 (50%), Gaps = 9/110 (8%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIELGQ--S 358
           GC  N  DSE M GLL  N + +T ++ +A   ++N+C  ++S  E+  ++  EL Q   
Sbjct: 10  GCPKNLVDSEVMLGLLRENNFTITNNEANADALIVNTCGFIESAKEESIRHIFELAQYKE 69

Query: 359 RG--IHVVVAGCVPQGAPKSGYLHGLS----IVGVQQIDRIVEVVEXTLK 490
           RG    ++V GC+ Q   K   L  +     I+G   +  IVEVV   L+
Sbjct: 70  RGKCKALIVTGCLAQRYSKE-LLEEIPEIDVILGPGHVSNIVEVVNHALE 118


>UniRef50_A4EC80 Cluster: Putative uncharacterized protein; n=1;
           Collinsella aerofaciens ATCC 25986|Rep: Putative
           uncharacterized protein - Collinsella aerofaciens ATCC
           25986
          Length = 449

 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 28/86 (32%), Positives = 44/86 (51%), Gaps = 6/86 (6%)
 Frame = +2

Query: 155 PGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHF 331
           P    +Y+ T GCA N  D++ M  LL A GY+   D  DA + ++N+C+ + S   +  
Sbjct: 4   PLGSVLYI-TLGCAKNEVDTDRMRSLLTAAGYEEAFDPQDADIAIVNTCSFLASATSESI 62

Query: 332 KNEIELGQS-----RGIHVVVAGCVP 394
           +  +EL        R   +V+ GCVP
Sbjct: 63  ETTLELANEVQDGVRSCPIVMCGCVP 88


>UniRef50_A6NSZ3 Cluster: Putative uncharacterized protein; n=1;
           Bacteroides capillosus ATCC 29799|Rep: Putative
           uncharacterized protein - Bacteroides capillosus ATCC
           29799
          Length = 471

 Score = 47.6 bits (108), Expect = 2e-04
 Identities = 19/50 (38%), Positives = 32/50 (64%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           +V T+GC  N +DSE + G L   GY  T+D+ +A + ++N+C ++  AE
Sbjct: 37  FVDTYGCQQNEADSERIRGYLKEMGYGFTQDEKEAAVIVINTCAIREHAE 86


>UniRef50_Q9YBR9 Cluster: MiaB homolog; n=2; Desulfurococcales|Rep:
           MiaB homolog - Aeropyrum pernix
          Length = 450

 Score = 47.2 bits (107), Expect = 2e-04
 Identities = 29/110 (26%), Positives = 55/110 (50%), Gaps = 4/110 (3%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           ++T Y++ +GC+ +  D+  MA  L   GY+      DA + L+N+C V+   E      
Sbjct: 17  SRTYYLEVYGCSLSEFDALIMASRLEEAGYRRVARPEDADVILVNTCAVRLDTEQRIAER 76

Query: 341 IE--LGQSRGIHVVVAGCVPQGAP--KSGYLHGLSIVGVQQIDRIVEVVE 478
           +E    Q      VVAGC+ +  P   +  +   S++  Q ++R+++ V+
Sbjct: 77  LEKLRLQLPDRKYVVAGCLVKARPGLVARLVPEASLLAPQAVERVLDAVD 126


>UniRef50_Q823A0 Cluster: MiaB-like tRNA modifying enzyme YliG; n=7;
           Chlamydiales|Rep: MiaB-like tRNA modifying enzyme YliG -
           Chlamydophila caviae
          Length = 460

 Score = 46.8 bits (106), Expect = 3e-04
 Identities = 29/111 (26%), Positives = 53/111 (47%), Gaps = 8/111 (7%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE- 346
           I+  + GC+ N  DSE M G+L   GY+ TE   +A   +LN+C     A D  K+ ++ 
Sbjct: 18  IHFISLGCSRNLVDSEVMLGILLKAGYEATETLEEADYLILNTCAFLKAARDESKDYLQR 77

Query: 347 --LGQSRGIHVVVAGCV-----PQGAPKSGYLHGLSIVGVQQIDRIVEVVE 478
               +     +++ GC+      +  P   Y+H   ++G   ++ I+  +E
Sbjct: 78  IIKAKKESAKIILTGCMVSKHKEELKPWLPYIH--YVLGSGDVEHILSAIE 126


>UniRef50_Q1JY65 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=1; Desulfuromonas acetoxidans DSM 684|Rep:
           TRNA-i(6)A37 modification enzyme MiaB - Desulfuromonas
           acetoxidans DSM 684
          Length = 444

 Score = 46.8 bits (106), Expect = 3e-04
 Identities = 29/84 (34%), Positives = 43/84 (51%), Gaps = 5/84 (5%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE----DH 328
           +++ Y++T+GC  N  DSE++  LL    Y   E    A L LLN+C+V+  AE     H
Sbjct: 2   SKSFYLETFGCQMNVVDSEWIVNLLGQIDYHPVETPQQADLILLNTCSVRDKAERKVYGH 61

Query: 329 FKNEIELGQSR-GIHVVVAGCVPQ 397
             +   L   R  + + V GCV Q
Sbjct: 62  LSHFKPLKDQRPDLILAVGGCVAQ 85


>UniRef50_Q1FEI6 Cluster: Putative uncharacterized protein; n=2;
           Clostridium|Rep: Putative uncharacterized protein -
           Clostridium phytofermentans ISDg
          Length = 440

 Score = 46.4 bits (105), Expect = 3e-04
 Identities = 34/115 (29%), Positives = 58/115 (50%), Gaps = 9/115 (7%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-- 343
           I+  + GC  N  DSE M GL+   G++LT D+ +A + ++N+C     A++   N I  
Sbjct: 3   IFFISLGCDKNLVDSEVMLGLIRDRGFELTNDESEADIIVVNTCCFIHDAKEESINTILE 62

Query: 344 --ELGQSRGIH-VVVAGCVPQGAPKSGYLHGL----SIVGVQQIDRIVEVVEXTL 487
             E  +S  +  ++V GC+ Q   K   L  +    +++G    D I EV++  L
Sbjct: 63  MAEYKKSGSLKGLIVTGCLAQ-RYKEDILAEIPEVDALLGTTSYDAITEVIDKVL 116


>UniRef50_Q3ZYS0 Cluster: TRNA-i(6)A37 thiotransferase enzyme MiaB;
           n=3; Dehalococcoides|Rep: TRNA-i(6)A37 thiotransferase
           enzyme MiaB - Dehalococcoides sp. (strain CBDB1)
          Length = 418

 Score = 46.0 bits (104), Expect = 5e-04
 Identities = 23/62 (37%), Positives = 35/62 (56%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELG 352
           Y+ T GC  N ++S+ +  L    GY L +   DA+L L+NSC V+  AE+   N + L 
Sbjct: 5   YLWTIGCQMNQAESDRLGRLFELWGYSLADKAEDAELVLVNSCVVREHAENKVVNRLHLL 64

Query: 353 QS 358
           +S
Sbjct: 65  RS 66


>UniRef50_A6GID8 Cluster: MiaB-like tRNA modifying enzyme YliG,
           TIGR01125; n=1; Plesiocystis pacifica SIR-1|Rep:
           MiaB-like tRNA modifying enzyme YliG, TIGR01125 -
           Plesiocystis pacifica SIR-1
          Length = 251

 Score = 45.6 bits (103), Expect = 6e-04
 Identities = 28/96 (29%), Positives = 50/96 (52%), Gaps = 11/96 (11%)
 Frame = +2

Query: 152 VPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDH 328
           V G + +Y  + GC  N  D+E M G++ ANG++L +D  +A   ++N+C  + +  ++ 
Sbjct: 18  VSGPKKVYFVSLGCPKNQVDTEVMLGVVQANGHQLVDDPSEADTLVVNTCGFIDAAKQES 77

Query: 329 FKNEIEL--------GQSRGI--HVVVAGCVPQGAP 406
               +EL        G +  +   +VVAGC+ Q  P
Sbjct: 78  IDTILELAAVKAEAAGDASVVDKRLVVAGCLSQRYP 113


>UniRef50_A0L887 Cluster: MiaB-like tRNA modifying enzyme YliG; n=1;
           Magnetococcus sp. MC-1|Rep: MiaB-like tRNA modifying
           enzyme YliG - Magnetococcus sp. (strain MC-1)
          Length = 487

 Score = 45.6 bits (103), Expect = 6e-04
 Identities = 27/90 (30%), Positives = 44/90 (48%), Gaps = 5/90 (5%)
 Frame = +2

Query: 143 ESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           E +     T+ V + GC+ N  DSE M G     GY L  D  +A L ++N+C   + AE
Sbjct: 30  EQLANAKGTVGVISLGCSKNTVDSEQMLGRFVREGYLLVADPLEADLLVVNTCGFIADAE 89

Query: 323 DHFKNEI-ELGQSRGIH----VVVAGCVPQ 397
              +  I E+   + ++    ++V GC+ Q
Sbjct: 90  RESRESIDEMAHIKQLYPHKKLIVTGCLSQ 119


>UniRef50_A1HR14 Cluster: RNA modification enzyme, MiaB family; n=1;
           Thermosinus carboxydivorans Nor1|Rep: RNA modification
           enzyme, MiaB family - Thermosinus carboxydivorans Nor1
          Length = 432

 Score = 45.2 bits (102), Expect = 8e-04
 Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ-- 355
           T GC  N  ++E + GL    GY +      A ++++N+C+V    E   +  I      
Sbjct: 8   TLGCKVNQFETEVIEGLFKQRGYTIVSFDEPADVYVINTCSVTHLGEKKSRQLIRRAARV 67

Query: 356 SRGIHVVVAGCVPQGAP-KSGYLHGLS-IVGVQQIDRIVEVVE 478
           +    +V  GC  Q +P K   + G+  IVG Q   RIV++VE
Sbjct: 68  NPEAVIVATGCYAQVSPDKVAAIPGVDVIVGTQDRGRIVDLVE 110


>UniRef50_Q2AFA0 Cluster: Putative uncharacterized protein; n=1;
           Halothermothrix orenii H 168|Rep: Putative
           uncharacterized protein - Halothermothrix orenii H 168
          Length = 438

 Score = 44.8 bits (101), Expect = 0.001
 Identities = 25/82 (30%), Positives = 41/82 (50%), Gaps = 2/82 (2%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           T+   T GC  N+ ++E M G+    GYK+ +    A ++++NSCTV + A    +    
Sbjct: 4   TVAFHTLGCKVNHYETEAMMGIFEEAGYKVVDFDDRADVYIINSCTVTNEAARKSRQLAR 63

Query: 347 LGQSRGIHVVVA--GCVPQGAP 406
             + +    VVA  GC  Q +P
Sbjct: 64  KARRKNPEAVVALVGCYAQVSP 85


>UniRef50_A6CGG9 Cluster: Probable MiaB protein-putative
           tRNA-thiotransferase; n=1; Planctomyces maris DSM
           8797|Rep: Probable MiaB protein-putative
           tRNA-thiotransferase - Planctomyces maris DSM 8797
          Length = 510

 Score = 44.8 bits (101), Expect = 0.001
 Identities = 20/51 (39%), Positives = 32/51 (62%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           +Y++T GC  N  DSE +   L   GY+LT++  +A+  L N+C+V+  AE
Sbjct: 34  LYIETVGCQMNMLDSELVVADLRKRGYELTQNVKEAETILFNTCSVREHAE 84


>UniRef50_Q5QP48 Cluster: CDK5 regulatory subunit associated protein
           1; n=6; Eutheria|Rep: CDK5 regulatory subunit associated
           protein 1 - Homo sapiens (Human)
          Length = 510

 Score = 44.8 bits (101), Expect = 0.001
 Identities = 21/90 (23%), Positives = 48/90 (53%)
 Frame = +2

Query: 74  KNVSVRSKKREKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYK 253
           K+ S   +K   +  +    ++++ ++   + +Y++T+GC  N +D+E    +L  +GY 
Sbjct: 70  KSASAPQEKLSSEVEDPPPYLMMDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYL 129

Query: 254 LTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
            T +  +A + LL +C+++  AE    N +
Sbjct: 130 RTSNLQEADVILLVTCSIREKAEQTIWNRL 159


>UniRef50_Q0W344 Cluster: Putative 2-methylthioadenine synthetase;
           n=1; uncultured methanogenic archaeon RC-I|Rep: Putative
           2-methylthioadenine synthetase - Uncultured methanogenic
           archaeon RC-I
          Length = 404

 Score = 44.8 bits (101), Expect = 0.001
 Identities = 29/95 (30%), Positives = 46/95 (48%), Gaps = 2/95 (2%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           T  +Y++T+GC  N +DS  +   + A+G  +     +A + ++N+C V      H  N 
Sbjct: 2   TMRVYIETYGCTANEADSAGIRDAVLASGGAIASSPEEADVIVVNTCAVTG----HTANS 57

Query: 341 IELGQSR--GIHVVVAGCVPQGAPKSGYLHGLSIV 439
           +    SR  G  V+VAGC+    P  G L G   V
Sbjct: 58  MLRAVSRFPGKRVLVAGCLAVAEP--GRLKGYEFV 90


>UniRef50_Q96SZ6 Cluster: CDK5 regulatory subunit-associated protein
           1; n=37; Bilateria|Rep: CDK5 regulatory
           subunit-associated protein 1 - Homo sapiens (Human)
          Length = 601

 Score = 44.8 bits (101), Expect = 0.001
 Identities = 21/90 (23%), Positives = 48/90 (53%)
 Frame = +2

Query: 74  KNVSVRSKKREKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYK 253
           K+ S   +K   +  +    ++++ ++   + +Y++T+GC  N +D+E    +L  +GY 
Sbjct: 70  KSASAPQEKLSSEVEDPPPYLMMDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYL 129

Query: 254 LTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
            T +  +A + LL +C+++  AE    N +
Sbjct: 130 RTSNLQEADVILLVTCSIREKAEQTIWNRL 159


>UniRef50_Q54KV4 Cluster: Putative uncharacterized protein; n=1;
           Dictyostelium discoideum AX4|Rep: Putative
           uncharacterized protein - Dictyostelium discoideum AX4
          Length = 607

 Score = 44.4 bits (100), Expect = 0.001
 Identities = 16/51 (31%), Positives = 33/51 (64%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           ++++T+GC  N SD E +  ++ ++GY ++ D   A +  LN+C+++  AE
Sbjct: 109 VWIETYGCQMNVSDEEVICSIMKSSGYTISNDFNTADIVFLNTCSIRENAE 159


>UniRef50_A7D1M3 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Halorubrum lacusprofundi ATCC 49239|Rep: MiaB-like tRNA
           modifying enzyme - Halorubrum lacusprofundi ATCC 49239
          Length = 434

 Score = 44.4 bits (100), Expect = 0.001
 Identities = 21/75 (28%), Positives = 38/75 (50%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           T +++T+GC+ N  +S  +   L   G++  +   DA + +LN+CTV    E +     E
Sbjct: 3   TYHIETYGCSSNRGESREIERALRDGGHRPADGPEDADVAILNTCTVVEKTERNMLRRAE 62

Query: 347 LGQSRGIHVVVAGCV 391
             +     +VV GC+
Sbjct: 63  ELEDVTAELVVTGCM 77


>UniRef50_O67016 Cluster: UPF0004 protein aq_849; n=2; Aquifex
           aeolicus|Rep: UPF0004 protein aq_849 - Aquifex aeolicus
          Length = 432

 Score = 44.4 bits (100), Expect = 0.001
 Identities = 26/75 (34%), Positives = 42/75 (56%), Gaps = 1/75 (1%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE-DHFKNEIE 346
           I V + GCA N  DSE + G L   G +LT +  +A + ++N+C    PA+ +  +  +E
Sbjct: 3   IGVVSLGCAKNLVDSEILLGKLKGAGVELTPNPEEADVIIVNTCGFIEPAKLESIETILE 62

Query: 347 LGQSRGIHVVVAGCV 391
             +S G  V+V GC+
Sbjct: 63  FAES-GKEVIVMGCL 76


>UniRef50_A6FYG6 Cluster: tRNA-i(6)A37 thiotransferase enzyme MiaB;
           n=1; Plesiocystis pacifica SIR-1|Rep: tRNA-i(6)A37
           thiotransferase enzyme MiaB - Plesiocystis pacifica
           SIR-1
          Length = 486

 Score = 44.0 bits (99), Expect = 0.002
 Identities = 19/57 (33%), Positives = 32/57 (56%)
 Frame = +2

Query: 155 PGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED 325
           P    +Y++T+GC  N +D+  + G L  +G+       +A L L+N+C V+  AED
Sbjct: 33  PHAPRVYMETFGCQMNEADTALVLGRLRQDGWVRVTSPAEADLVLVNTCAVREKAED 89


>UniRef50_A6DR68 Cluster: Putative Fe-S oxidoreductase; n=1;
           Lentisphaera araneosa HTCC2155|Rep: Putative Fe-S
           oxidoreductase - Lentisphaera araneosa HTCC2155
          Length = 437

 Score = 44.0 bits (99), Expect = 0.002
 Identities = 30/110 (27%), Positives = 54/110 (49%), Gaps = 4/110 (3%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           T+   V T GC  N S+S  M   L   G+ + + K ++ + ++N+CTV + A+   +N 
Sbjct: 9   TKKASVYTLGCRLNQSESSVMEQGLKEQGFDIVDFKGESNIAIVNTCTVTARADSDCRNV 68

Query: 341 IE--LGQSRGIHVVVAGCVPQ-GAPKSGYLHGLS-IVGVQQIDRIVEVVE 478
           I   + ++    V V GC  Q G      + G+  I+G Q    +++ V+
Sbjct: 69  IRSYIRRNPDAFVAVVGCYSQMGYKTLAEIEGVDLIIGNQDKMSVLDYVK 118


>UniRef50_A7I5K8 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Candidatus Methanoregula boonei 6A8|Rep: MiaB-like tRNA
           modifying enzyme - Methanoregula boonei (strain 6A8)
          Length = 430

 Score = 44.0 bits (99), Expect = 0.002
 Identities = 21/75 (28%), Positives = 37/75 (49%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y++T+GC +N  D+  +  +L   G  +     DA   ++N+CTV  P E      +  
Sbjct: 25  VYIETYGCRYNFGDTANLVAVLKHYGSTVVPAPEDADAVVVNTCTVVGPTERRMLRRLSA 84

Query: 350 GQSRGIHVVVAGCVP 394
            Q + +   V GC+P
Sbjct: 85  LQEKPL--FVTGCMP 97


>UniRef50_Q09316 Cluster: CDK5RAP1-like protein; n=3; Bilateria|Rep:
           CDK5RAP1-like protein - Caenorhabditis elegans
          Length = 547

 Score = 44.0 bits (99), Expect = 0.002
 Identities = 23/82 (28%), Positives = 46/82 (56%), Gaps = 4/82 (4%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +T+   T+GC  N SD E +  ++   G+  ++ K +A + LL +C+++  AE    N++
Sbjct: 79  RTVCYVTYGCQMNVSDMEIVRSIMTKYGFVESDKKENADIVLLMTCSIRDGAEKKVWNQL 138

Query: 344 ELGQSRGIH----VVVAGCVPQ 397
           +L +S  ++    V V GC+ +
Sbjct: 139 KLIRSNSVNKGQIVGVLGCMAE 160


>UniRef50_Q6A908 Cluster: Conserved protein, radical SAM superfamily
           protein; n=11; Actinomycetales|Rep: Conserved protein,
           radical SAM superfamily protein - Propionibacterium
           acnes
          Length = 481

 Score = 43.6 bits (98), Expect = 0.002
 Identities = 24/82 (29%), Positives = 43/82 (52%), Gaps = 5/82 (6%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC----TVKSPAEDHFK 334
           T+++ + GCA N+ DSE +A  + A G++L +D  +A+  ++N+C      K  + D   
Sbjct: 9   TVHLVSMGCARNDVDSEELAARMEAGGFRLVDDPAEAETVVVNTCGFIEQAKKDSVDTLL 68

Query: 335 NEIEL-GQSRGIHVVVAGCVPQ 397
              +L G      VV  GC+ +
Sbjct: 69  AAADLKGNGITTSVVAVGCMAE 90


>UniRef50_A6LKT7 Cluster: MiaB-like tRNA modifying enzyme; n=2;
           Thermotogaceae|Rep: MiaB-like tRNA modifying enzyme -
           Thermosipho melanesiensis BI429
          Length = 429

 Score = 43.6 bits (98), Expect = 0.002
 Identities = 23/78 (29%), Positives = 39/78 (50%), Gaps = 2/78 (2%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           + + T+GC  N  +SE M   L   GY +   + ++ ++++NSC V + A    K +I  
Sbjct: 3   VSIITYGCKLNQYESELMTERLENEGYVVVNGEVESDIYVINSCVVTNEATRKVKQQIRR 62

Query: 350 GQSR--GIHVVVAGCVPQ 397
            + R     +VV GC  Q
Sbjct: 63  LKKRFPDSKIVVTGCYSQ 80


>UniRef50_Q1IPQ5 Cluster: Putative uncharacterized protein; n=1;
           Acidobacteria bacterium Ellin345|Rep: Putative
           uncharacterized protein - Acidobacteria bacterium
           (strain Ellin345)
          Length = 504

 Score = 43.2 bits (97), Expect = 0.003
 Identities = 24/73 (32%), Positives = 40/73 (54%), Gaps = 5/73 (6%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKNEIEL----G 352
           GC  N  DSE M GLLA NG ++T    DA + ++N+C+ + +  ++     +E+     
Sbjct: 27  GCPKNLVDSEVMMGLLATNGAEITARAEDADIIVVNTCSFIDTAKQESVDTILEMAGHKA 86

Query: 353 QSRGIHVVVAGCV 391
             R   ++VAGC+
Sbjct: 87  TGRAQKLIVAGCL 99


>UniRef50_A6GE00 Cluster: tRNA 2-methylthioadenosine synthase-like
           protein; n=1; Plesiocystis pacifica SIR-1|Rep: tRNA
           2-methylthioadenosine synthase-like protein -
           Plesiocystis pacifica SIR-1
          Length = 453

 Score = 43.2 bits (97), Expect = 0.003
 Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 2/83 (2%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           + V T GC  N ++S+ +A  L A G++L      A L+LLNSC +   A+   +  +  
Sbjct: 3   VAVDTHGCRLNQAESDAIAEQLRAAGHELVPRAELADLYLLNSCAITHEADADARAAVRR 62

Query: 350 GQ--SRGIHVVVAGCVPQGAPKS 412
            +  +  + V+V GC     P++
Sbjct: 63  ARRHNPAVEVIVTGCHANAEPEA 85


>UniRef50_A5UQQ2 Cluster: MiaB-like tRNA modifying enzyme YliG; n=4;
           Chloroflexaceae|Rep: MiaB-like tRNA modifying enzyme
           YliG - Roseiflexus sp. RS-1
          Length = 472

 Score = 43.2 bits (97), Expect = 0.003
 Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 3/78 (3%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDH---FKNEI 343
           ++ T GC  N  DSE M+ +LAA G+       DA + ++N+C+  + A +       E+
Sbjct: 4   HIITLGCPKNQVDSEGMSSILAAQGHTPVAHADDADVVVVNTCSFIAAAREETLDVLREV 63

Query: 344 ELGQSRGIHVVVAGCVPQ 397
              ++ G ++V AGC+ +
Sbjct: 64  AARKTPGQYLVAAGCMAE 81


>UniRef50_UPI00006CFA0B Cluster: RNA modification enzyme, MiaB
           family; n=1; Tetrahymena thermophila SB210|Rep: RNA
           modification enzyme, MiaB family - Tetrahymena
           thermophila SB210
          Length = 604

 Score = 42.7 bits (96), Expect = 0.004
 Identities = 17/58 (29%), Positives = 34/58 (58%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           +++T+GC  N SD+E ++G+L   G+    +  +A +  LN+C ++  AE+     +E
Sbjct: 79  FIETYGCQMNESDTEIISGILQKAGFVRESNLDNADIVFLNTCAIREGAENKIWKRLE 136


>UniRef50_Q8H0V1 Cluster: CDK5RAP1-like protein; n=9;
           Viridiplantae|Rep: CDK5RAP1-like protein - Arabidopsis
           thaliana (Mouse-ear cress)
          Length = 640

 Score = 42.7 bits (96), Expect = 0.004
 Identities = 21/70 (30%), Positives = 38/70 (54%), Gaps = 1/70 (1%)
 Frame = +2

Query: 116 PEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYK-LTEDKWDAQLWLL 292
           PE   +  L+S +     IY +T+GC  N +D E +  ++  +GYK +  D   A++  +
Sbjct: 113 PETESESTLDSDIASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFV 172

Query: 293 NSCTVKSPAE 322
           N+C ++  AE
Sbjct: 173 NTCAIRENAE 182


>UniRef50_Q8RB61 Cluster: 2-methylthioadenine synthetase; n=19;
           Clostridia|Rep: 2-methylthioadenine synthetase -
           Thermoanaerobacter tengcongensis
          Length = 437

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 28/103 (27%), Positives = 55/103 (53%), Gaps = 4/103 (3%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSR 361
           T GC  N  ++E MA L    GY++ +    A ++++N+C+V + ++   +  I   +++
Sbjct: 8   TLGCKVNQYETEVMAELFRKAGYEIVDFDEIADVYVINTCSVTARSDMKSRQMIRKTRNK 67

Query: 362 G--IHVVVAGCVPQGAPKSGY-LHGLSIV-GVQQIDRIVEVVE 478
                VV  GC  Q +P   + +  + IV G +  D+IV++V+
Sbjct: 68  NPDAIVVAVGCYVQVSPDEVFSMPEVDIVIGTKDKDKIVDLVK 110


>UniRef50_Q67NX5 Cluster: 2-methylthioadenine synthetase; n=1;
           Symbiobacterium thermophilum|Rep: 2-methylthioadenine
           synthetase - Symbiobacterium thermophilum
          Length = 485

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 38/108 (35%), Positives = 56/108 (51%), Gaps = 8/108 (7%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-ELGQ--S 358
           GCA N  D+E M GLL   GY++T    +A + ++N+C     A+    + I E  Q  +
Sbjct: 27  GCAKNLVDTESMIGLLRNTGYQITNRAEEADVLVVNTCGFIDAAKQESVDAILEAAQHKT 86

Query: 359 RG--IHVVVAGC-VPQ-GAPKSGYLHGL-SIVGVQQIDRIVEVVEXTL 487
           RG    +VVAGC VP+ G   +  +  + ++VG     RI EVV   L
Sbjct: 87  RGRCQALVVAGCMVPRYGEELAREIPEIDALVGTADYPRIGEVVAGIL 134


>UniRef50_Q1VHX9 Cluster: Putative uncharacterized protein; n=1;
           Psychroflexus torquis ATCC 700755|Rep: Putative
           uncharacterized protein - Psychroflexus torquis ATCC
           700755
          Length = 118

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 16/51 (31%), Positives = 30/51 (58%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED 325
           ++KT+GC  N  DSE ++G+   +G      +  A +  +N+CT++  A+D
Sbjct: 23  FIKTFGCQMNEHDSERISGMFELDGMSKASSEEFADILFVNTCTIRENADD 73


>UniRef50_A7B2V4 Cluster: Putative uncharacterized protein; n=1;
           Ruminococcus gnavus ATCC 29149|Rep: Putative
           uncharacterized protein - Ruminococcus gnavus ATCC 29149
          Length = 494

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 20/51 (39%), Positives = 29/51 (56%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPA 319
           T +V T+GC  N  DSE + G+L   GY   E++  A   + N+CTV+  A
Sbjct: 54  TFHVTTFGCQMNARDSEKLTGILEQIGYVEEEEENQADFVIYNTCTVRENA 104


>UniRef50_A6P2W1 Cluster: Putative uncharacterized protein; n=1;
           Bacteroides capillosus ATCC 29799|Rep: Putative
           uncharacterized protein - Bacteroides capillosus ATCC
           29799
          Length = 434

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 25/90 (27%), Positives = 44/90 (48%), Gaps = 2/90 (2%)
 Frame = +2

Query: 176 VKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ 355
           + T GC  N  +++ +   L   G+ L   + +A  +++N+CTV + ++   +N I   +
Sbjct: 5   IYTLGCKVNQYETQALETELLRRGHTLVPFEDEADAYIINTCTVTAVSDRKSRNAIRRAK 64

Query: 356 SRGIHVVVA--GCVPQGAPKSGYLHGLSIV 439
            R    VVA  GC  Q AP      G+ +V
Sbjct: 65  KRNPAAVVAVCGCYAQTAPDDVAALGVDLV 94


>UniRef50_A1I9T0 Cluster: MiaB-like tRNA modifying enzyme YliG; n=1;
           Candidatus Desulfococcus oleovorans Hxd3|Rep: MiaB-like
           tRNA modifying enzyme YliG - Candidatus Desulfococcus
           oleovorans Hxd3
          Length = 440

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 30/110 (27%), Positives = 52/110 (47%), Gaps = 8/110 (7%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKNEIE 346
           +++ + GCA N  DSE M G  AA G  + +D   A + ++N+C  ++    +     + 
Sbjct: 3   VHLTSLGCAKNQVDSELMLGAFAAEGLTVCDDPAGADVLVVNTCAFIEDAVNEAVDTILA 62

Query: 347 LG--QSRGI--HVVVAGCVPQ--GAPKSGYLHGLS-IVGVQQIDRIVEVV 475
           L   +S G    ++V GC+P+  G   +G L       G     R++E V
Sbjct: 63  LARYKSEGSCRRLIVCGCLPERFGEELAGALPEADFFFGTGAYHRVIEAV 112


>UniRef50_A2SQZ8 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Methanocorpusculum labreanum Z|Rep: MiaB-like tRNA
           modifying enzyme - Methanocorpusculum labreanum (strain
           ATCC 43576 / DSM 4855 / Z)
          Length = 416

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 21/76 (27%), Positives = 36/76 (47%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           ++Y +T+GC +N  D+E +  +    G        +A   L+N+C V    E H    ++
Sbjct: 16  SLYTETYGCTYNAGDTEKLMEIARNQGCVPASSAEEADAILINTCVVIDKTEQHMYERLD 75

Query: 347 LGQSRGIHVVVAGCVP 394
           L    G  + V GC+P
Sbjct: 76  L--YAGKLLFVTGCLP 89


>UniRef50_Q9VGZ1 Cluster: CDK5RAP1-like protein; n=2;
           Sophophora|Rep: CDK5RAP1-like protein - Drosophila
           melanogaster (Fruit fly)
          Length = 583

 Score = 42.3 bits (95), Expect = 0.006
 Identities = 16/59 (27%), Positives = 34/59 (57%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           ++ + +GC  N +D+E +  +L  NGY   ++  +A + +L +C V+  AE   +N ++
Sbjct: 94  VHFEVYGCQMNTNDTEVVFSILKENGYLRCQEPEEADVIMLVTCAVRDGAEQRIRNRLK 152


>UniRef50_A5ZQ90 Cluster: Putative uncharacterized protein; n=2;
           Ruminococcus|Rep: Putative uncharacterized protein -
           Ruminococcus obeum ATCC 29174
          Length = 445

 Score = 41.9 bits (94), Expect = 0.007
 Identities = 23/75 (30%), Positives = 41/75 (54%), Gaps = 5/75 (6%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-ELGQSRG 364
           GC  N +DSE M GLL  NG+++ + + +A   ++N+C     A++   N I E+ + + 
Sbjct: 9   GCDKNLADSEEMLGLLTGNGHEIVDSEEEADAIVINTCCFIHDAKEESVNTILEMAEYKK 68

Query: 365 IH----VVVAGCVPQ 397
                 ++V GC+ Q
Sbjct: 69  TGPCKILIVTGCMAQ 83


>UniRef50_Q6MAB2 Cluster: Putative 2-methylthioadenine synthetase;
           n=1; Candidatus Protochlamydia amoebophila UWE25|Rep:
           Putative 2-methylthioadenine synthetase - Protochlamydia
           amoebophila (strain UWE25)
          Length = 434

 Score = 41.5 bits (93), Expect = 0.010
 Identities = 22/85 (25%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           T    + T GC  N  +S+     L   GY+  ++   A + ++N+CTV   A+   ++ 
Sbjct: 5   TNKFKIITLGCRTNQYESQAYQNQLLRMGYQEAKEGEKADICIVNTCTVTESADSSSRHA 64

Query: 341 IE--LGQSRGIHVVVAGCVPQGAPK 409
           I     +++G  ++VAGC  +  P+
Sbjct: 65  IRQLARENQGTQLLVAGCFAERQPE 89


>UniRef50_Q3ACX5 Cluster: MiaB-like tRNA modifying enzyme YliG,
           TIGR01125; n=1; Carboxydothermus hydrogenoformans
           Z-2901|Rep: MiaB-like tRNA modifying enzyme YliG,
           TIGR01125 - Carboxydothermus hydrogenoformans (strain
           Z-2901 / DSM 6008)
          Length = 438

 Score = 41.5 bits (93), Expect = 0.010
 Identities = 21/78 (26%), Positives = 44/78 (56%), Gaps = 3/78 (3%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIEL 349
           ++ + GC  N +DSE + G+L + GY  + +  ++ L ++N+C  + +  E+  +  + L
Sbjct: 4   FILSLGCTKNQADSEVIMGILESKGYVRSLNPEESDLLIVNTCGFIAAAIEESIEEILNL 63

Query: 350 GQSR--GIHVVVAGCVPQ 397
              +  G  ++VAGC+ Q
Sbjct: 64  VHLKKPGQKILVAGCLVQ 81


>UniRef50_Q892R4 Cluster: Fe-S oxidoreductase; n=3; Clostridium|Rep:
           Fe-S oxidoreductase - Clostridium tetani
          Length = 433

 Score = 41.1 bits (92), Expect = 0.013
 Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ-- 355
           T GC  N  ++E M      +GY + +    A ++++N+CTV +  +   +  I   +  
Sbjct: 7   TLGCRVNQYETEAMTEKFIKSGYDIVDFDKLADVYVINTCTVTNMGDKKSRQMISRARRI 66

Query: 356 SRGIHVVVAGCVPQGAP-KSGYLHGLSIV-GVQQIDRIVEVVE 478
           +    + V GC  Q AP K   + G+ +V G +    IV+ VE
Sbjct: 67  NNNATIAVVGCYSQVAPEKVSQIPGVDVVIGTRNKGDIVKKVE 109


>UniRef50_Q1JYQ2 Cluster: MiaB-like tRNA modifying enzyme; n=2;
           Desulfuromonadales|Rep: MiaB-like tRNA modifying enzyme
           - Desulfuromonas acetoxidans DSM 684
          Length = 428

 Score = 41.1 bits (92), Expect = 0.013
 Identities = 25/106 (23%), Positives = 52/106 (49%), Gaps = 4/106 (3%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           + + T GC  N  +S  M  +L   GY++   +  A+L ++N+CTV S  +   +  +  
Sbjct: 4   VSIVTLGCKANQFESAAMERMLREQGYQIVPFEQGAELVIVNTCTVTSATDAQSRKLVRR 63

Query: 350 GQ--SRGIHVVVAGCVPQGAPKS-GYLHG-LSIVGVQQIDRIVEVV 475
            +  +    +VV GC  Q  P+    L G + ++G  +   +++++
Sbjct: 64  ARRLNGQCRIVVTGCYAQIQPQQIAELPGVMYVIGNSEKQDLIDIL 109


>UniRef50_A6ESE6 Cluster: Possible 2-methylthioadenine synthetase;
           n=22; cellular organisms|Rep: Possible
           2-methylthioadenine synthetase - unidentified
           eubacterium SCB49
          Length = 449

 Score = 41.1 bits (92), Expect = 0.013
 Identities = 23/78 (29%), Positives = 40/78 (51%), Gaps = 2/78 (2%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSR 361
           T GC  N S++  +A      GY+  + K +A ++++N+C+V   A+  FK+ ++  Q  
Sbjct: 11  TLGCKLNFSETSTIARDFTKEGYERVDFKEEADIYVVNTCSVTENADKRFKSIVKQAQKV 70

Query: 362 GIHVVVA--GCVPQGAPK 409
                VA  GC  Q  P+
Sbjct: 71  NPDAFVAAIGCYAQLKPE 88


>UniRef50_O83735 Cluster: UPF0004 protein TP_0754; n=2;
           Treponema|Rep: UPF0004 protein TP_0754 - Treponema
           pallidum
          Length = 456

 Score = 41.1 bits (92), Expect = 0.013
 Identities = 21/81 (25%), Positives = 40/81 (49%), Gaps = 4/81 (4%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI- 343
           T + +T+GC  N ++S  +  LL A G+    D     + ++N+C+V+  AE      + 
Sbjct: 2   TYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRLG 61

Query: 344 ---ELGQSRGIHVVVAGCVPQ 397
               L + R   +++ GC+ Q
Sbjct: 62  LFSSLKKKRAFFIILMGCMAQ 82


>UniRef50_Q2LVR5 Cluster: TRNA 2-methylthioadenosine synthase-like
           protein; n=1; Syntrophus aciditrophicus SB|Rep: TRNA
           2-methylthioadenosine synthase-like protein - Syntrophus
           aciditrophicus (strain SB)
          Length = 451

 Score = 40.7 bits (91), Expect = 0.017
 Identities = 28/109 (25%), Positives = 52/109 (47%), Gaps = 4/109 (3%)
 Frame = +2

Query: 176 VKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE--L 349
           + T GC  N  +SE +   L   GY +      A  +++N+CTV +      +  I   +
Sbjct: 21  IATLGCKVNQYESEGLGEALTRRGYTMVPFSSVADCYIINTCTVTARTNYQSRQIIRKAI 80

Query: 350 GQSRGIHVVVAGCVPQGAP-KSGYLHGLSIV-GVQQIDRIVEVVEXTLK 490
             +    +VV GC  Q AP +   + G++++ G  + D+I +++   LK
Sbjct: 81  RNNPEAVIVVTGCYAQTAPAEIAGIPGVTLIAGHAEKDQIPDLIARLLK 129


>UniRef50_Q2J750 Cluster: Putative uncharacterized protein; n=2;
           Frankia|Rep: Putative uncharacterized protein - Frankia
           sp. (strain CcI3)
          Length = 523

 Score = 40.7 bits (91), Expect = 0.017
 Identities = 18/40 (45%), Positives = 25/40 (62%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC 301
           T GC+ N  DSE +A  L A+G++L  D  DA   L+N+C
Sbjct: 13  TLGCSRNEVDSEELAARLGADGWELVSDAADADAVLVNTC 52


>UniRef50_Q04UA3 Cluster: 2-methylthioadenine synthetase; n=4;
           Leptospira|Rep: 2-methylthioadenine synthetase -
           Leptospira borgpetersenii serovar Hardjo-bovis (strain
           JB197)
          Length = 449

 Score = 40.7 bits (91), Expect = 0.017
 Identities = 24/84 (28%), Positives = 43/84 (51%), Gaps = 5/84 (5%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           T  +Y++T+GC  N  DS  ++ L+    Y  + D  ++ +  LN+C ++  A     N 
Sbjct: 10  TGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNR 69

Query: 341 IE-LG--QSRGIHVV--VAGCVPQ 397
           ++ LG  + R   +V  V GC+ Q
Sbjct: 70  LQSLGYLKKRNPELVIGVLGCMAQ 93


>UniRef50_A0LV11 Cluster: MiaB-like tRNA modifying enzyme YliG; n=1;
           Acidothermus cellulolyticus 11B|Rep: MiaB-like tRNA
           modifying enzyme YliG - Acidothermus cellulolyticus
           (strain ATCC 43068 / 11B)
          Length = 475

 Score = 40.7 bits (91), Expect = 0.017
 Identities = 28/114 (24%), Positives = 53/114 (46%), Gaps = 2/114 (1%)
 Frame = +2

Query: 152 VPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHF 331
           +P ++T+ +   GCA N+ D+E +A  L   G++LTE    A + ++N+C     A+   
Sbjct: 1   MPASRTVRLIRLGCARNDVDAEELAARLVDAGWRLTEAP-SADVTVVNTCGFIEAAKQES 59

Query: 332 KNEIELGQSRGIHVVVAGCVPQ--GAPKSGYLHGLSIVGVQQIDRIVEVVEXTL 487
            + +         VV  GC+ +  GA  +  +   +I+       I + +E  L
Sbjct: 60  IDTLLEAADGSTRVVAVGCLAERYGAALADAMPEATILSFDDYPVIAQRLEDVL 113


>UniRef50_Q6MLC6 Cluster: Putative uncharacterized protein; n=1;
           Bdellovibrio bacteriovorus|Rep: Putative uncharacterized
           protein - Bdellovibrio bacteriovorus
          Length = 453

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 29/111 (26%), Positives = 51/111 (45%), Gaps = 9/111 (8%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y+ T+GC  N +D+E M  LL    +    D   A L ++NSC+V+        +E+  
Sbjct: 23  VYISTYGCQMNVNDTERMYALLEMQNFVPVTDPKKASLIIINSCSVREKPVHKVYSEVGT 82

Query: 350 -----GQSRGIHVVVAGCVPQGAPKSGYLHGLSIV----GVQQIDRIVEVV 475
                 ++  + + V GCV Q   K   +    ++    G  QID + ++V
Sbjct: 83  YKYMKRKNPELKIGVGGCVGQ-QEKENLMKTQPMIDFVFGTDQIDSLPQLV 132


>UniRef50_Q6AQ27 Cluster: Putative uncharacterized protein; n=3;
           Deltaproteobacteria|Rep: Putative uncharacterized
           protein - Desulfotalea psychrophila
          Length = 443

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 22/52 (42%), Positives = 30/52 (57%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           GCA N  DSE + G L   G+++T D+ DA L L+N+C    PA +    EI
Sbjct: 9   GCAKNLVDSEVVLGCLRDAGWEMT-DEQDADLLLVNTCGFIQPAVEEAVEEI 59


>UniRef50_Q6AIZ5 Cluster: Putative uncharacterized protein; n=1;
           Desulfotalea psychrophila|Rep: Putative uncharacterized
           protein - Desulfotalea psychrophila
          Length = 434

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 30/113 (26%), Positives = 52/113 (46%), Gaps = 8/113 (7%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE- 346
           I + T GC  N  +S   +  L+  GYK+     +A   ++N+CTV + A    ++ I  
Sbjct: 4   ISITTLGCKVNQFESASFSDNLSQTGYKIVGHNEEADYIIINTCTVTAAASAQSRHSIRH 63

Query: 347 -LGQSRGIHVVVAGC-VPQGAPKSGYLHGL-----SIVGVQQIDRIVEVVEXT 484
            L  S    +++ GC V  GA +   +  L      I+G    D++V+ +  T
Sbjct: 64  ALRLSPTAKIIITGCYVEIGAEEIQAIEELRGREYHIIGNSCKDQVVDTIRST 116


>UniRef50_Q3A8J5 Cluster: 2-methylthioadenine synthetase; n=2;
           Desulfuromonadales|Rep: 2-methylthioadenine synthetase -
           Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
          Length = 455

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 31/105 (29%), Positives = 53/105 (50%), Gaps = 8/105 (7%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDH----FKNEIELGQ 355
           GCA N  D+E M G L  + +++T D+  A + ++N+C   S A++         IE  +
Sbjct: 18  GCAKNLVDAEVMLGYLPQDRFEITTDEAQADIIIVNTCGFISDAKEESVETLLEAIEYKK 77

Query: 356 SRGIH-VVVAGCVPQ--GAPKSGYLHGLSI-VGVQQIDRIVEVVE 478
           S     +VV GC+ Q      +  L  + I +G   + RI+E++E
Sbjct: 78  SGNCTLLVVTGCLSQRYAEDMAKELPEVDILLGTGDVPRILELIE 122


>UniRef50_Q1ISD7 Cluster: MiaB-like tRNA modifying enzyme; n=2;
           Acidobacteria|Rep: MiaB-like tRNA modifying enzyme -
           Acidobacteria bacterium (strain Ellin345)
          Length = 495

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 25/95 (26%), Positives = 45/95 (47%), Gaps = 4/95 (4%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFK---N 337
           + +V+ +GC    +D   +   L   G        DA++ +LN+CTV + A+   +    
Sbjct: 40  SFFVENFGCRATQADGAAIERQLLEKGLARGSSAIDAEVVVLNTCTVTASADQDARAAIR 99

Query: 338 EIELGQSRGIHVVVAGCVPQGAPKS-GYLHGLSIV 439
            I+ G      ++V GC  Q AP+    + G+S+V
Sbjct: 100 RIKRGNPEA-RIIVTGCYAQRAPEEISRIEGVSLV 133


>UniRef50_A7CWE3 Cluster: MiaB-like tRNA modifying enzyme YliG; n=1;
           Opitutaceae bacterium TAV2|Rep: MiaB-like tRNA modifying
           enzyme YliG - Opitutaceae bacterium TAV2
          Length = 473

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 33/119 (27%), Positives = 57/119 (47%), Gaps = 13/119 (10%)
 Frame = +2

Query: 161 TQTIYVK--TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHF 331
           T TI V   + GCA N  DSE M G L   G  +  +   A + ++N+C+ + S  E+  
Sbjct: 2   TTTIKVSLVSLGCAKNLVDSEIMIGHLHQAGMSVVPETDQADVVIVNTCSFIDSSKEESI 61

Query: 332 KNEIELGQSRGI-------HVVVAGCVPQGAPK---SGYLHGLSIVGVQQIDRIVEVVE 478
            + +   Q+RG+        ++VAGC+ Q   K   +      + +G+ Q+  I  ++E
Sbjct: 62  NHILAAHQARGLSKRRKEQKLIVAGCMSQRFSKELPAAMPEVDAFIGLDQLTGIAPIIE 120


>UniRef50_A5GE34 Cluster: MiaB-like tRNA modifying enzyme; n=5;
           Desulfuromonadales|Rep: MiaB-like tRNA modifying enzyme
           - Geobacter uraniumreducens Rf4
          Length = 444

 Score = 40.3 bits (90), Expect = 0.023
 Identities = 23/81 (28%), Positives = 40/81 (49%), Gaps = 2/81 (2%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           T+ + T GC  N  +S  M+  L  +G+++      A ++++N+CTV S  +   +  I 
Sbjct: 11  TVAITTLGCKINQFESAAMSEALGKDGFQVIPFDDVADIYVINTCTVTSRTDAESRRLIR 70

Query: 347 LG--QSRGIHVVVAGCVPQGA 403
               Q+    +VV GC  Q A
Sbjct: 71  RASRQNPSARIVVTGCYAQVA 91


>UniRef50_Q73JG6 Cluster: MiaB-like tRNA modifying enzyme YliG,
           TIGR01125; n=1; Treponema denticola|Rep: MiaB-like tRNA
           modifying enzyme YliG, TIGR01125 - Treponema denticola
          Length = 467

 Score = 39.9 bits (89), Expect = 0.030
 Identities = 23/74 (31%), Positives = 41/74 (55%), Gaps = 4/74 (5%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGI 367
           GCA N  D+E + G++    +K T D  +A L ++NSC   + A++   N +   Q++  
Sbjct: 5   GCAKNQVDAELIIGIMENLSWKNTSDPDEADLIIVNSCGFINSAKEESINAVL--QAKAA 62

Query: 368 H----VVVAGCVPQ 397
           H    V++AGC+ +
Sbjct: 63  HPKAKVLLAGCLAE 76


>UniRef50_Q1AW39 Cluster: Putative uncharacterized protein; n=1;
           Rubrobacter xylanophilus DSM 9941|Rep: Putative
           uncharacterized protein - Rubrobacter xylanophilus
           (strain DSM 9941 / NBRC 16129)
          Length = 445

 Score = 39.9 bits (89), Expect = 0.030
 Identities = 24/81 (29%), Positives = 41/81 (50%), Gaps = 5/81 (6%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +T  ++T+GC  N  DS+ M  ++   GY   +   DA L +LN+C V+  A +  +  +
Sbjct: 29  RTACIRTFGCQMNVHDSDRMRRMILDAGYAEVQRYEDADLVILNTCYVRENAVNRIRGHL 88

Query: 344 -ELG----QSRGIHVVVAGCV 391
            EL     + R   V + GC+
Sbjct: 89  GELNRLRREGRVKKVALTGCI 109


>UniRef50_A7GZE8 Cluster: 2-methylthioadenine synthetase; n=14;
           Epsilonproteobacteria|Rep: 2-methylthioadenine
           synthetase - Campylobacter curvus 525.92
          Length = 444

 Score = 39.9 bits (89), Expect = 0.030
 Identities = 24/79 (30%), Positives = 45/79 (56%), Gaps = 3/79 (3%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIE 346
           +++ + GC  N  DSE M G L  + Y+LT +  +A + ++N+C  + S  E+  +  +E
Sbjct: 13  LHLVSLGCNKNLVDSEIMLGRL--SNYELTNETREADVIIVNTCGFIASAKEESVRVILE 70

Query: 347 LGQSR--GIHVVVAGCVPQ 397
           +  ++  G  +VV GC+ Q
Sbjct: 71  MADAKKQGATLVVTGCLMQ 89


>UniRef50_A5TX86 Cluster: tRNA 2-methylthioadenosine synthase; n=3;
           Fusobacterium nucleatum|Rep: tRNA 2-methylthioadenosine
           synthase - Fusobacterium nucleatum subsp. polymorphum
           ATCC 10953
          Length = 435

 Score = 39.9 bits (89), Expect = 0.030
 Identities = 32/106 (30%), Positives = 49/106 (46%), Gaps = 7/106 (6%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI----EL 349
           T+GC  N ++S  +  +    GY +TE+  +A    LN+CTV+  A      ++     L
Sbjct: 8   TYGCQMNVNESAKIKKIFQNLGYDVTEEIDNADAVFLNTCTVREGAATQIFGKLGELKAL 67

Query: 350 GQSRGIHVVVAGCV--PQGAPKSGYLHGLSIV-GVQQIDRIVEVVE 478
            + RG  + V GC    QG         + IV G Q I RI + +E
Sbjct: 68  KEKRGTIIGVTGCFAQEQGEELVKKFPIIDIVMGNQNIGRIPQAIE 113


>UniRef50_A1VF04 Cluster: RNA modification enzyme, MiaB family; n=4;
           Desulfovibrionaceae|Rep: RNA modification enzyme, MiaB
           family - Desulfovibrio vulgaris subsp. vulgaris (strain
           DP4)
          Length = 476

 Score = 39.9 bits (89), Expect = 0.030
 Identities = 23/83 (27%), Positives = 43/83 (51%), Gaps = 5/83 (6%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +T +++T+GC  N +DS+++A  L   G+       +A+L ++N+C+V+   E    + +
Sbjct: 31  RTFHIETFGCQMNVNDSDWLARALMERGFS-PAPFGEARLTIVNTCSVRDKPEQKVYSLL 89

Query: 344 -----ELGQSRGIHVVVAGCVPQ 397
                  G+     V V GCV Q
Sbjct: 90  GRIRQATGKKPDAFVAVGGCVAQ 112


>UniRef50_Q01DS1 Cluster: Predicted Fe-S oxidoreductase; n=1;
           Ostreococcus tauri|Rep: Predicted Fe-S oxidoreductase -
           Ostreococcus tauri
          Length = 548

 Score = 39.9 bits (89), Expect = 0.030
 Identities = 16/53 (30%), Positives = 31/53 (58%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           + ++V+T+GC  N +DS+ +  LL    + +     DA + L+N+C ++  AE
Sbjct: 43  ERVFVETYGCQMNANDSDVVRALLVEAKHAIASSASDATVVLVNTCAIRENAE 95


>UniRef50_A0UWB9 Cluster: Radical SAM; n=1; Clostridium
           cellulolyticum H10|Rep: Radical SAM - Clostridium
           cellulolyticum H10
          Length = 416

 Score = 39.5 bits (88), Expect = 0.040
 Identities = 20/68 (29%), Positives = 33/68 (48%)
 Frame = +2

Query: 191 CAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGIH 370
           C+    D   +   L+ANGY++ ED+  A   +  +C   +       NEIE  +S    
Sbjct: 13  CSRRQMDMVKLESYLSANGYEVVEDEKQADQIVYTTCGFINETAQVAFNEIERLKSLPAE 72

Query: 371 VVVAGCVP 394
           ++V GC+P
Sbjct: 73  LIVTGCLP 80


>UniRef50_Q6L1Y8 Cluster: Hypothetical oxidoreductase; n=4;
           Thermoplasmatales|Rep: Hypothetical oxidoreductase -
           Picrophilus torridus
          Length = 426

 Score = 39.5 bits (88), Expect = 0.040
 Identities = 18/74 (24%), Positives = 36/74 (48%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y +++GC    S++      +  +G +L +D   A + ++ +C V    EDH    I  
Sbjct: 29  VYFESYGCTLEKSEAALYVNKMLQDGGELVDDPERADVSVIGTCVVIKHTEDHMLKRIGE 88

Query: 350 GQSRGIHVVVAGCV 391
              +  +V+V GC+
Sbjct: 89  LSKKSRNVLVLGCL 102


>UniRef50_UPI00015BB1B3 Cluster: RNA modification enzyme, MiaB
           family; n=1; Ignicoccus hospitalis KIN4/I|Rep: RNA
           modification enzyme, MiaB family - Ignicoccus hospitalis
           KIN4/I
          Length = 423

 Score = 39.1 bits (87), Expect = 0.053
 Identities = 21/79 (26%), Positives = 38/79 (48%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           IY +T+GCA    ++E +   L + GY++     +A   ++ +CTV+S  E      I+ 
Sbjct: 3   IYYETYGCAVMLGEAERVLEELKSKGYEVVGRPEEADASIIFTCTVRSETEQRMAWRIKE 62

Query: 350 GQSRGIHVVVAGCVPQGAP 406
                  ++V GC+    P
Sbjct: 63  LCKASKKLIVTGCLASAQP 81


>UniRef50_A6C349 Cluster: Putative uncharacterized protein; n=1;
           Planctomyces maris DSM 8797|Rep: Putative
           uncharacterized protein - Planctomyces maris DSM 8797
          Length = 436

 Score = 39.1 bits (87), Expect = 0.053
 Identities = 25/89 (28%), Positives = 44/89 (49%), Gaps = 3/89 (3%)
 Frame = +2

Query: 155 PGT-QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHF 331
           PG  +T  + T GC  N  +++ +   L  NGY+   +   A L ++N+CTV +  +   
Sbjct: 9   PGKDKTCQLVTLGCKVNQYETQLVKEALEKNGYREAGEAETADLCVVNTCTVTATGDSKG 68

Query: 332 KNEI-ELGQSR-GIHVVVAGCVPQGAPKS 412
           +  I  L ++  G  ++V GC     PK+
Sbjct: 69  RKLIRNLAKNNPGTKILVMGCYATRDPKT 97


>UniRef50_Q5SHW2 Cluster: Putative uncharacterized protein TTHA1618;
           n=2; Thermus thermophilus|Rep: Putative uncharacterized
           protein TTHA1618 - Thermus thermophilus (strain HB8 /
           ATCC 27634 / DSM 579)
          Length = 436

 Score = 38.7 bits (86), Expect = 0.070
 Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 3/80 (3%)
 Frame = +2

Query: 179 KTWGCAHNNSDSEYMAGLLAANGYKLTE-DKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ 355
           +T GC  N  ++E + G L A   ++   +   A L ++NSC V + AE   + E+   +
Sbjct: 6   RTLGCKVNQVETEALLGFLKALEPEVVPLEAGGADLVVINSCAVTTTAEADTRKEVRRAR 65

Query: 356 SRGIH--VVVAGCVPQGAPK 409
               H  +VV GC  + AP+
Sbjct: 66  RYNPHAFIVVTGCYAELAPE 85


>UniRef50_Q1PXT1 Cluster: Putative uncharacterized protein; n=1;
           Candidatus Kuenenia stuttgartiensis|Rep: Putative
           uncharacterized protein - Candidatus Kuenenia
           stuttgartiensis
          Length = 447

 Score = 38.7 bits (86), Expect = 0.070
 Identities = 20/71 (28%), Positives = 39/71 (54%), Gaps = 2/71 (2%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE--LGQ 355
           T+GC  N  +++ +   L A G+     +  A ++++N+CTV S +++  +N I+    +
Sbjct: 13  TFGCKVNQYETQALRESLIAKGFMEISPEMAADVYVINTCTVTSASDEKSRNYIKRLKKK 72

Query: 356 SRGIHVVVAGC 388
           S    +VV GC
Sbjct: 73  SPKSSIVVTGC 83


>UniRef50_Q9HP07 Cluster: Putative uncharacterized protein; n=3;
           Halobacteriaceae|Rep: Putative uncharacterized protein -
           Halobacterium salinarium (Halobacterium halobium)
          Length = 432

 Score = 38.7 bits (86), Expect = 0.070
 Identities = 19/73 (26%), Positives = 34/73 (46%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELG 352
           +++T+GC  N  +S  +   L   G+   E   DA + +LN+CTV    E +     +  
Sbjct: 5   HIETYGCTSNRGESRDIERRLRDAGHHKVETAADADVAILNTCTVVEKTERNMLRRAKEL 64

Query: 353 QSRGIHVVVAGCV 391
                 ++V GC+
Sbjct: 65  ADETADLIVTGCM 77


>UniRef50_A1IFA3 Cluster: TRNA 2-methylthioadenosine synthase-like
           protein; n=1; Candidatus Desulfococcus oleovorans
           Hxd3|Rep: TRNA 2-methylthioadenosine synthase-like
           protein - Candidatus Desulfococcus oleovorans Hxd3
          Length = 451

 Score = 38.3 bits (85), Expect = 0.092
 Identities = 18/50 (36%), Positives = 29/50 (58%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKS 313
           ++  +KT GC  N  +SE +A  L + G+ L +    A L ++N+CTV S
Sbjct: 2   KSFIIKTLGCKVNQFESEAIAAALISEGWCLADAGGPADLCIVNTCTVTS 51


>UniRef50_P54462 Cluster: UPF0004 protein yqeV; n=38;
           Bacillales|Rep: UPF0004 protein yqeV - Bacillus subtilis
          Length = 451

 Score = 38.3 bits (85), Expect = 0.092
 Identities = 26/108 (24%), Positives = 55/108 (50%), Gaps = 4/108 (3%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE 346
           T+   T GC  N+ ++E +  L    GY+  + +  A ++++N+CTV +  +   +  I 
Sbjct: 3   TVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQVIR 62

Query: 347 --LGQSRGIHVVVAGCVPQGAP-KSGYLHGLSI-VGVQQIDRIVEVVE 478
             + Q+    + V GC  Q +P +   + G+ I VG Q  ++++  ++
Sbjct: 63  RAIRQNPDGVICVTGCYAQTSPAEIMAIPGVDIVVGTQDREKMLGYID 110


>UniRef50_Q6MGT1 Cluster: Putative uncharacterized protein; n=1;
           Bdellovibrio bacteriovorus|Rep: Putative uncharacterized
           protein - Bdellovibrio bacteriovorus
          Length = 457

 Score = 37.9 bits (84), Expect = 0.12
 Identities = 22/75 (29%), Positives = 40/75 (53%), Gaps = 5/75 (6%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIELG---- 352
           GC  N  DSE MAG L  +GY++  +   A   ++N+C  ++   ++  +  +++     
Sbjct: 17  GCPKNLVDSEIMAGTLMKDGYEVVGEADQADTVIVNTCGFIEDSKKESIQRILDMSDLKQ 76

Query: 353 QSRGIHVVVAGCVPQ 397
           + +   VVVAGC+ Q
Sbjct: 77  EGKIKKVVVAGCLTQ 91


>UniRef50_Q607P8 Cluster: Putative uncharacterized protein; n=1;
           Methylococcus capsulatus|Rep: Putative uncharacterized
           protein - Methylococcus capsulatus
          Length = 436

 Score = 37.9 bits (84), Expect = 0.12
 Identities = 24/75 (32%), Positives = 38/75 (50%), Gaps = 2/75 (2%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           I +++ GC  N ++ E  A    A G++L  +  DA L +LNSC V + A    +  I  
Sbjct: 3   INLQSLGCRLNEAELESWAREFQAAGHRLVSETGDADLIVLNSCAVTAEAVRKSRQMIRR 62

Query: 350 GQ--SRGIHVVVAGC 388
            Q  S    +V++GC
Sbjct: 63  TQRLSPRARLVLSGC 77


>UniRef50_A6QCC6 Cluster: tRNA modifying enzyme; n=3;
           Epsilonproteobacteria|Rep: tRNA modifying enzyme -
           Sulfurovum sp. (strain NBC37-1)
          Length = 439

 Score = 37.9 bits (84), Expect = 0.12
 Identities = 22/85 (25%), Positives = 45/85 (52%), Gaps = 3/85 (3%)
 Frame = +2

Query: 152 VPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDH 328
           +P  + +++ + GC  N  DSE M G L    Y++T+D  +A + ++N+C  + +  E+ 
Sbjct: 1   MPSRKKLHLISLGCTKNLVDSEVMLGRL--KEYEITDDNTEADVIIVNTCGFIDAAKEES 58

Query: 329 FKNEIELGQSR--GIHVVVAGCVPQ 397
               + L   R     +V++GC+ +
Sbjct: 59  INTVLNLHDERKEDSILVMSGCLSE 83


>UniRef50_A5TU09 Cluster: 2-methylthioadenine synthetase; n=3;
           Fusobacterium nucleatum|Rep: 2-methylthioadenine
           synthetase - Fusobacterium nucleatum subsp. polymorphum
           ATCC 10953
          Length = 435

 Score = 37.9 bits (84), Expect = 0.12
 Identities = 21/74 (28%), Positives = 37/74 (50%), Gaps = 2/74 (2%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ-- 355
           T GC  N  ++E +   L   GY+    +  + ++++NSCTV S A+   +N +   +  
Sbjct: 11  TLGCKVNQYETESIKNQLIKRGYEEVPFEDKSDIYIINSCTVTSIADRKTRNMLRRAKKI 70

Query: 356 SRGIHVVVAGCVPQ 397
           +    V+V GC  Q
Sbjct: 71  NPDAKVIVTGCYAQ 84


>UniRef50_A0W5N6 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Geobacter lovleyi SZ|Rep: MiaB-like tRNA modifying
           enzyme - Geobacter lovleyi SZ
          Length = 442

 Score = 37.9 bits (84), Expect = 0.12
 Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 2/79 (2%)
 Frame = +2

Query: 176 VKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQ 355
           V T GC  N  ++  M   +   G++  +    A L+L+NSCTV + ++   +  I   +
Sbjct: 14  VATLGCKVNQFETADMIEQMQTAGWQQVKFSEVADLYLINSCTVTARSDAESRRLIRRAR 73

Query: 356 SRGIH--VVVAGCVPQGAP 406
               H  +V  GC  Q AP
Sbjct: 74  RTNPHAKIVATGCYAQVAP 92


>UniRef50_A0LIM0 Cluster: MiaB-like tRNA modifying enzyme YliG; n=1;
           Syntrophobacter fumaroxidans MPOB|Rep: MiaB-like tRNA
           modifying enzyme YliG - Syntrophobacter fumaroxidans
           (strain DSM 10017 / MPOB)
          Length = 444

 Score = 37.9 bits (84), Expect = 0.12
 Identities = 25/75 (33%), Positives = 38/75 (50%), Gaps = 5/75 (6%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIELGQSRG 364
           GCA N  DSE M   L   GY++T +   A L L+N+C  ++S   +     ++L   + 
Sbjct: 12  GCAKNLVDSESMVSQLIELGYEMTPEVSQAALILVNTCGFLESAVRESIDTVLQLAGYKA 71

Query: 365 I----HVVVAGCVPQ 397
                 +VVAGC+ Q
Sbjct: 72  SGSCEKLVVAGCMVQ 86


>UniRef50_A5FQT7 Cluster: MiaB-like tRNA modifying enzyme; n=3;
           Dehalococcoides|Rep: MiaB-like tRNA modifying enzyme -
           Dehalococcoides sp. BAV1
          Length = 416

 Score = 37.5 bits (83), Expect = 0.16
 Identities = 20/64 (31%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLT--EDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           I + T GC  N +++E M    A  GY L   +D WD  +++LN+CTV   A+   + ++
Sbjct: 4   IALDTLGCKLNQAETEAMGREFAQAGYHLVSPQDNWD--IYILNTCTVTHVADRKARYQM 61

Query: 344 ELGQ 355
            + +
Sbjct: 62  RIAR 65


>UniRef50_A1IDX9 Cluster: TRNA-i(6)A37 modification enzyme MiaB;
           n=1; Candidatus Desulfococcus oleovorans Hxd3|Rep:
           TRNA-i(6)A37 modification enzyme MiaB - Candidatus
           Desulfococcus oleovorans Hxd3
          Length = 466

 Score = 37.5 bits (83), Expect = 0.16
 Identities = 16/50 (32%), Positives = 28/50 (56%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           Y+ T GC  N  DS  ++ +L A G++       A L  +N+CT+++ A+
Sbjct: 5   YIHTIGCQMNVYDSSQLSAILTAMGHRSVNAPEQADLVFVNTCTIRAKAK 54


>UniRef50_Q1Q4S9 Cluster: Similar to 2-methylthioadenine synthetase;
           n=1; Candidatus Kuenenia stuttgartiensis|Rep: Similar to
           2-methylthioadenine synthetase - Candidatus Kuenenia
           stuttgartiensis
          Length = 437

 Score = 37.1 bits (82), Expect = 0.21
 Identities = 16/51 (31%), Positives = 31/51 (60%)
 Frame = +2

Query: 149 VVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC 301
           ++  ++T+ +   GC  N  D+E M G +AANG  + +   DA++ ++N+C
Sbjct: 3   MISKSKTVALINLGCTKNLVDAEEMLGRIAANGSTICQYPEDAEVLVVNTC 53


>UniRef50_Q04PJ5 Cluster: 2-methylthioadenine synthetase; n=4;
           Leptospira|Rep: 2-methylthioadenine synthetase -
           Leptospira borgpetersenii serovar Hardjo-bovis (strain
           JB197)
          Length = 443

 Score = 37.1 bits (82), Expect = 0.21
 Identities = 20/77 (25%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +T+   T GC  N  +S+ +   L+ +G++  E     ++ ++N+CTV + A+   +N I
Sbjct: 9   RTVLFNTLGCRLNFFESDGLFSSLSKHGFRSVEVGEHPEVVIINTCTVTNKADSKNRNTI 68

Query: 344 E--LGQSRGIHVVVAGC 388
              + +  G  + V GC
Sbjct: 69  RNAIKKFPGSQIWVTGC 85


>UniRef50_A4S5H4 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
           Predicted protein - Ostreococcus lucimarinus CCE9901
          Length = 450

 Score = 37.1 bits (82), Expect = 0.21
 Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIELGQ--S 358
           GC  N  D E M G L   G+ +T+D   A   ++NSC  V+    +  +  +E  Q  +
Sbjct: 5   GCPKNTVDGEVMLGDLHGAGFDVTDDHESADAIVINSCGFVEDAKNESVEAILEASQLAN 64

Query: 359 RGIHVVVAGCVPQ 397
               ++V GC+ Q
Sbjct: 65  GSKKIIVTGCLAQ 77


>UniRef50_P56130 Cluster: UPF0004 protein HP_0285; n=10;
           Epsilonproteobacteria|Rep: UPF0004 protein HP_0285 -
           Helicobacter pylori (Campylobacter pylori)
          Length = 418

 Score = 37.1 bits (82), Expect = 0.21
 Identities = 20/73 (27%), Positives = 38/73 (52%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL 349
           +Y KT+GC  N  D++ M+  L    +  T ++ +A + ++NSCTV + A+   ++  + 
Sbjct: 4   VYFKTFGCRTNLFDTQVMSENL--KDFSTTLEEQEADIIIINSCTVTNGADSAVRSYAKK 61

Query: 350 GQSRGIHVVVAGC 388
                  V+  GC
Sbjct: 62  MARLDKEVLFTGC 74


>UniRef50_A7H5G3 Cluster: MiaB-like tRNA modifying enzyme YliG,
           TIGR01125; n=10; Campylobacter|Rep: MiaB-like tRNA
           modifying enzyme YliG, TIGR01125 - Campylobacter jejuni
           subsp. doylei 269.97
          Length = 455

 Score = 36.7 bits (81), Expect = 0.28
 Identities = 24/79 (30%), Positives = 43/79 (54%), Gaps = 3/79 (3%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIE 346
           +Y+ + GC  N  DSE M G L+A  Y+L ++   A + ++N+C  + S  ++     ++
Sbjct: 20  LYLMSLGCNKNLVDSEIMLGRLSA--YELCDEPSKADVLIVNTCGFIDSAKKESINAILD 77

Query: 347 LGQSR--GIHVVVAGCVPQ 397
           L + R     +VV GC+ Q
Sbjct: 78  LHEQRKKDSLLVVTGCLMQ 96


>UniRef50_A5ZXQ4 Cluster: Putative uncharacterized protein; n=1;
           Ruminococcus obeum ATCC 29174|Rep: Putative
           uncharacterized protein - Ruminococcus obeum ATCC 29174
          Length = 128

 Score = 36.7 bits (81), Expect = 0.28
 Identities = 18/51 (35%), Positives = 29/51 (56%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPA 319
           T  + T+GC  N   SE +AG++   GY   +D  +A + + N+CTV+  A
Sbjct: 42  TYCLTTFGCQMNEKQSEAVAGIMDEIGYH-RQDNEEADVVIYNTCTVRENA 91


>UniRef50_A4XLD9 Cluster: MiaB-like tRNA modifying enzyme YliG; n=2;
           Clostridia|Rep: MiaB-like tRNA modifying enzyme YliG -
           Caldicellulosiruptor saccharolyticus (strain ATCC 43494
           / DSM 8903)
          Length = 440

 Score = 36.7 bits (81), Expect = 0.28
 Identities = 28/105 (26%), Positives = 50/105 (47%), Gaps = 8/105 (7%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC----TVKSPAEDHFKNEIELGQ 355
           GC  N  DSE M G     G+++T +  DA + ++N+C      K  + D      E   
Sbjct: 10  GCNKNLVDSEIMMGACKEAGFEITPNAEDADVIVINTCGFINDAKQESIDTILEMAEYKN 69

Query: 356 SRGIHVVVAGCVPQGAPKSGYLHGL----SIVGVQQIDRIVEVVE 478
            +   ++V GC+ Q   K   L  L    +I+GV+++ ++  V++
Sbjct: 70  KKCKFLIVTGCLSQ-RYKDDILKELPEVDAILGVKEMLKLPNVIK 113


>UniRef50_A4M7C8 Cluster: MiaB-like tRNA modifying enzyme; n=1;
           Petrotoga mobilis SJ95|Rep: MiaB-like tRNA modifying
           enzyme - Petrotoga mobilis SJ95
          Length = 434

 Score = 36.7 bits (81), Expect = 0.28
 Identities = 30/102 (29%), Positives = 52/102 (50%), Gaps = 7/102 (6%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKW-DAQLWLLNSCTVKSPAEDHFKNEI-ELGQ 355
           T+GC  N ++S+ MA  L+ +   + E+K   + +++LN+C V S AE   +  I  L +
Sbjct: 9   TFGCKMNQAESQAMAEKLSPHFDIVFEEKMGKSDIYVLNTCAVTSEAERKVRQTIRRLKK 68

Query: 356 S-RGIHVVVAGCV----PQGAPKSGYLHGLSIVGVQQIDRIV 466
           S     ++  GC     P+   K G    L  +  +QIDR++
Sbjct: 69  SNENSKIIATGCYSVSDPEELKKVGADLVLGNLEKKQIDRLL 110


>UniRef50_Q7UK39 Cluster: Putative uncharacterized protein; n=2;
           Planctomycetaceae|Rep: Putative uncharacterized protein
           - Rhodopirellula baltica
          Length = 477

 Score = 36.3 bits (80), Expect = 0.37
 Identities = 25/79 (31%), Positives = 38/79 (48%), Gaps = 5/79 (6%)
 Frame = +2

Query: 176 VKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE--L 349
           V + GC  N  D+E M G L A+GY++ +    A   ++N+C     A D     I+  L
Sbjct: 36  VVSLGCPKNLVDTEQMLGRLDADGYRMVDSVDGADFVVVNTCGFIDSARDESMAAIDEML 95

Query: 350 GQSRG---IHVVVAGCVPQ 397
              R     +VVV GC+ +
Sbjct: 96  ALKRDGKLRNVVVTGCLAE 114


>UniRef50_Q49573 Cluster: UPF0004 protein in 16S RNA 5'region; n=2;
           Mycoplasma|Rep: UPF0004 protein in 16S RNA 5'region -
           Mycoplasma iowae
          Length = 438

 Score = 36.3 bits (80), Expect = 0.37
 Identities = 25/81 (30%), Positives = 43/81 (53%), Gaps = 4/81 (4%)
 Frame = +2

Query: 167 TIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWD--AQLWLLNSCTVKSPAEDHFKNE 340
           T  + T GC  N  +S  +   L  NG  L E  +D  A ++++N+CTV + A+   +  
Sbjct: 9   TFAIHTLGCKVNLFESNSIKNDLIMNG--LVEVPFDSKADVYIINTCTVTNKADAKSRLY 66

Query: 341 IELG--QSRGIHVVVAGCVPQ 397
           I+    Q++   ++VAGC+ Q
Sbjct: 67  IKRAHVQNKDAIIIVAGCMSQ 87


>UniRef50_Q2RKX1 Cluster: MiaB-like tRNA modifying enzyme; n=5;
           Clostridia|Rep: MiaB-like tRNA modifying enzyme -
           Moorella thermoacetica (strain ATCC 39073)
          Length = 450

 Score = 35.9 bits (79), Expect = 0.49
 Identities = 27/100 (27%), Positives = 48/100 (48%), Gaps = 4/100 (4%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGI 367
           GC  N ++ E +  L    GY++     +A ++++++CTV   ++   +  I        
Sbjct: 12  GCKVNQNEVEALKHLFQEAGYQVVPFPEEADVYVVHTCTVTHISDRKSRQLIRRAIRANP 71

Query: 368 HVVVA--GCVPQGAPKSGY-LHGLS-IVGVQQIDRIVEVV 475
             VVA  GC  Q AP     + G+  +VG +   R+VE+V
Sbjct: 72  EAVVAVTGCYAQVAPGEVLAIPGVDLVVGTRDRHRLVELV 111


>UniRef50_Q7MSY9 Cluster: MiaB-like tRNA modifying enzyme; n=4;
           Bacteroidales|Rep: MiaB-like tRNA modifying enzyme -
           Porphyromonas gingivalis (Bacteroides gingivalis)
          Length = 444

 Score = 35.1 bits (77), Expect = 0.86
 Identities = 23/89 (25%), Positives = 42/89 (47%), Gaps = 3/89 (3%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIEL--GQ 355
           T GC  N +++  +   LA  G +   +   A + ++N+C+V   A+   +N I     +
Sbjct: 16  TLGCKLNFAETSTIGKALAEQGVRPVREGEKADICVINTCSVTELADKKCRNAIRKLHKE 75

Query: 356 SRGIHVVVAGCVPQGAPKS-GYLHGLSIV 439
             G  ++V GC  Q  P+    + G+ IV
Sbjct: 76  HPGALMIVTGCYAQLKPEEIARIDGVDIV 104


>UniRef50_Q4W554 Cluster: MiaB-like tRNA modifying enzyme; n=6;
           Chlorobiaceae|Rep: MiaB-like tRNA modifying enzyme -
           Chlorobium tepidum
          Length = 446

 Score = 35.1 bits (77), Expect = 0.86
 Identities = 24/95 (25%), Positives = 44/95 (46%), Gaps = 3/95 (3%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI 343
           +++   T GC  N +++  +   L + G++L      A + ++++C V   AE   + +I
Sbjct: 4   KSVAAVTLGCKVNYAETSSIVDALVSQGWQLNAIDDGADVLIIHTCAVTGEAERKSRQQI 63

Query: 344 E--LGQSRGIHVVVAGCVPQGAPKS-GYLHGLSIV 439
              +    G  V V GC  Q  PK    + G+S V
Sbjct: 64  RKIIRNHPGSRVGVIGCYAQLDPKRIADIKGVSFV 98


>UniRef50_Q30XS8 Cluster: Putative uncharacterized protein; n=1;
           Desulfovibrio desulfuricans G20|Rep: Putative
           uncharacterized protein - Desulfovibrio desulfuricans
           (strain G20)
          Length = 435

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 2/77 (2%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELG 352
           Y  T GC  N  +++ +  +  A G+   +   +A L L+N+C V + A    +  +   
Sbjct: 9   YAATLGCKINQYETQALREVWQARGFTEVQSTAEADLVLVNTCAVTAKAVSDVRATVRQA 68

Query: 353 QSRG--IHVVVAGCVPQ 397
                   +VV GC  Q
Sbjct: 69  HRANPLARIVVTGCAAQ 85


>UniRef50_Q194H8 Cluster: TRNA-i(6)A37 thiotransferase enzyme MiaB;
           n=4; Clostridia|Rep: TRNA-i(6)A37 thiotransferase enzyme
           MiaB - Desulfitobacterium hafniense (strain DCB-2)
          Length = 447

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 19/89 (21%), Positives = 44/89 (49%), Gaps = 5/89 (5%)
 Frame = +2

Query: 146 SVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAED 325
           S+    + +    +GC  +  D++ +  + +  GY  +++   A L ++N+C V+  AE+
Sbjct: 2   SITKVPKKVVTLAYGCQMSERDADTLTEISSQKGYVRSQELEQADLIIVNTCCVRESAEN 61

Query: 326 HFKNEI-ELGQSR----GIHVVVAGCVPQ 397
               +I EL   +     + + ++GC+ Q
Sbjct: 62  KILGKIGELKHLKEANPQLKIAISGCMVQ 90


>UniRef50_Q0AXI3 Cluster: 2-methylthioadenine synthetase; n=1;
           Syntrophomonas wolfei subsp. wolfei str. Goettingen|Rep:
           2-methylthioadenine synthetase - Syntrophomonas wolfei
           subsp. wolfei (strain Goettingen)
          Length = 439

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 21/75 (28%), Positives = 37/75 (49%), Gaps = 5/75 (6%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI----ELGQ 355
           GC+ N  D+E M   L   G+++      A L ++N+C   +PA++     I    EL +
Sbjct: 9   GCSKNRVDTEVMMAALKKAGHRIVNSLERADLVVVNTCGFITPAKEESIEAIIETAELKK 68

Query: 356 SRGIH-VVVAGCVPQ 397
              +  ++ AGC+ Q
Sbjct: 69  KGSLQFLIAAGCLSQ 83


>UniRef50_Q03HM3 Cluster: Transcriptional regulator containing an
            AAA-type ATPase domain and a DNA-binding domain; n=1;
            Pediococcus pentosaceus ATCC 25745|Rep: Transcriptional
            regulator containing an AAA-type ATPase domain and a
            DNA-binding domain - Pediococcus pentosaceus (strain ATCC
            25745 / 183-1w)
          Length = 913

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 30/129 (23%), Positives = 59/129 (45%), Gaps = 3/129 (2%)
 Frame = +2

Query: 101  REKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYK-LTEDKWDA 277
            R+ KD  +I   I   ++P TQ IY K      N   +    G+  AN  K L  D    
Sbjct: 660  RQCKDINKIMDDIRSKIIPSTQVIYPKE--IKKNLIITCCFTGIGTANNVKNLLLDSMPE 717

Query: 278  QLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVA--GCVPQGAPKSGYLHGLSIVGVQQ 451
            ++     C +++   +  K+E ++     ++ ++A  G +  G PK+ Y+   S++   +
Sbjct: 718  EV----DCDIQAFEIERLKDEEQIAIFNKLYNILAVVGTIDPGLPKAPYISLESVISGNE 773

Query: 452  IDRIVEVVE 478
            ID+  + ++
Sbjct: 774  IDKFNDALQ 782


>UniRef50_A3JF75 Cluster: Putative uncharacterized protein; n=1;
           Marinobacter sp. ELB17|Rep: Putative uncharacterized
           protein - Marinobacter sp. ELB17
          Length = 183

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 27/99 (27%), Positives = 41/99 (41%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGI 367
           GC     DSE +   L  +GY +     DA + ++N+C     A+    + I    S   
Sbjct: 57  GCPKALVDSERILTQLRLDGYDVVPTYKDADIVVVNTCGFIDAAKQESLDAIGEAISENG 116

Query: 368 HVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEXT 484
            V+V GC+   A K    H   ++ V  +    EVV  T
Sbjct: 117 KVIVTGCMGLEADKIRETHP-GVLVVSNLHACEEVVRCT 154


>UniRef50_A1FEK1 Cluster: Putative uncharacterized protein; n=1;
           Pseudomonas putida W619|Rep: Putative uncharacterized
           protein - Pseudomonas putida W619
          Length = 259

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 22/73 (30%), Positives = 38/73 (52%), Gaps = 7/73 (9%)
 Frame = +2

Query: 260 EDKWDAQLWLLNSCTVKSPAEDHFKNEIE-------LGQSRGIHVVVAGCVPQGAPKSGY 418
           +D  ++ +W+ NS + K+  +D+    ++       LG  RG+H +VAG V Q AP+   
Sbjct: 120 KDCKESGVWVENSYSTKASYKDYLNELLQVHEGNNVLGHKRGVHQLVAGDVCQTAPEFFK 179

Query: 419 LHGLSIVGVQQID 457
            +G +IV     D
Sbjct: 180 NNGSAIVAFAYFD 192


>UniRef50_O66772 Cluster: UPF0004 protein aq_474; n=1; Aquifex
           aeolicus|Rep: UPF0004 protein aq_474 - Aquifex aeolicus
          Length = 410

 Score = 34.7 bits (76), Expect = 1.1
 Identities = 20/79 (25%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
 Frame = +2

Query: 179 KTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQS 358
           +T GC  N  D++ +       GY++   +  A ++++N+CTV    +   +  I   + 
Sbjct: 6   ETLGCRMNQFDTDLLKNKFIQKGYEVVSFEDMADVYVINTCTVTVGGDRSSRQAIYQAKR 65

Query: 359 RG--IHVVVAGCVPQGAPK 409
           R     VV  GC  Q  P+
Sbjct: 66  RNPKAIVVATGCYAQVNPQ 84


>UniRef50_Q29R15 Cluster: LP17019p; n=5; Sophophora|Rep: LP17019p -
           Drosophila melanogaster (Fruit fly)
          Length = 805

 Score = 34.3 bits (75), Expect = 1.5
 Identities = 17/61 (27%), Positives = 29/61 (47%)
 Frame = -2

Query: 416 SHFLVRPAVHSLLQQHVCHDSDQAQFHS*NDPQPGSLQYMNSTTKVAHPICLLSICSH*Q 237
           + F++RP      QQH  H   Q +  +  + +P + Q +  + +V H + L  I  H Q
Sbjct: 91  NQFIIRPIAPHQHQQHESHQEPQLRNFAAANSRPHAAQLLEQSQEVQHYVYLQDIMRHHQ 150

Query: 236 P 234
           P
Sbjct: 151 P 151


>UniRef50_Q4RNH8 Cluster: Chromosome undetermined SCAF15013, whole
            genome shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
            Chromosome undetermined SCAF15013, whole genome shotgun
            sequence - Tetraodon nigroviridis (Green puffer)
          Length = 2067

 Score = 33.9 bits (74), Expect = 2.0
 Identities = 12/20 (60%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
 Frame = +1

Query: 370  CCCSRLCT-AGRTKKWLPTW 426
            CCCSRLC+    T  WLP+W
Sbjct: 1215 CCCSRLCSPVSSTPLWLPSW 1234


>UniRef50_Q1NYL6 Cluster: TRNA-i(6)A37 thiotransferase enzyme MiaB;
           n=1; Candidatus Sulcia muelleri str. Hc (Homalodisca
           coagulata)|Rep: TRNA-i(6)A37 thiotransferase enzyme MiaB
           - Candidatus Sulcia muelleri str. Hc (Homalodisca
           coagulata)
          Length = 438

 Score = 33.9 bits (74), Expect = 2.0
 Identities = 19/69 (27%), Positives = 40/69 (57%), Gaps = 5/69 (7%)
 Frame = +2

Query: 200 NNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFK---NEIE--LGQSRG 364
           N SDSE ++ +L   G+  TE+  +A + L+N+C+++  +E       N+I+  + ++  
Sbjct: 1   NISDSEIVSSILNNKGFIKTENLKEANIILINTCSIRDKSEKKILLRINQIKFIIKKNND 60

Query: 365 IHVVVAGCV 391
           I + + GC+
Sbjct: 61  ILIGILGCM 69


>UniRef50_UPI00015BD265 Cluster: UPI00015BD265 related cluster; n=1;
           unknown|Rep: UPI00015BD265 UniRef100 entry - unknown
          Length = 411

 Score = 33.5 bits (73), Expect = 2.6
 Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 2/72 (2%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGI 367
           GC  N  D ++++  L  +GY+ +E      ++++N+C+V S A+   +  I   +    
Sbjct: 9   GCRMNQFDGDFISSWLLKHGYEKSE---IPDIYIINTCSVTSQADRSSRQAIYQAKKENP 65

Query: 368 H--VVVAGCVPQ 397
           +  V+  GC  Q
Sbjct: 66  NAIVIATGCYAQ 77


>UniRef50_Q9RYW7 Cluster: Putative uncharacterized protein; n=1;
           Deinococcus radiodurans|Rep: Putative uncharacterized
           protein - Deinococcus radiodurans
          Length = 504

 Score = 33.5 bits (73), Expect = 2.6
 Identities = 26/102 (25%), Positives = 43/102 (42%), Gaps = 2/102 (1%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGI 367
           GC     DSE +   L A GY++      A   ++N+C   +PA +   + I        
Sbjct: 29  GCPKALVDSERILTQLRAEGYEVAPSYEGADAVIVNTCGFITPAVEESLSAIGEALDATG 88

Query: 368 HVVVAGCVPQGAPKSGYLHG--LSIVGVQQIDRIVEVVEXTL 487
            V+V GC+ +   K    H    +I G + +D ++  V   L
Sbjct: 89  KVIVTGCLGERPEKIMERHPKVAAITGSEAVDDVMGHVRELL 130


>UniRef50_Q04ZD0 Cluster: 2-methylthioadenine synthetase; n=5;
           Leptospira|Rep: 2-methylthioadenine synthetase -
           Leptospira borgpetersenii serovar Hardjo-bovis (strain
           L550)
          Length = 439

 Score = 33.5 bits (73), Expect = 2.6
 Identities = 21/82 (25%), Positives = 37/82 (45%), Gaps = 4/82 (4%)
 Frame = +2

Query: 173 YVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKNEIEL 349
           Y+ T GC  N +DS  M   L   G+       ++    +N+CT ++S  E+  +  +  
Sbjct: 6   YITTLGCPKNTADSMSMHHSLLEEGFTPATFAEESDFHFINTCTFIQSATEETIQTILSA 65

Query: 350 GQSRGIH---VVVAGCVPQGAP 406
            Q +  +   +VV GC  +  P
Sbjct: 66  AQVKKQNHQKLVVVGCFAERYP 87


>UniRef50_A5GAH4 Cluster: Metallophosphoesterase precursor; n=1;
           Geobacter uraniumreducens Rf4|Rep:
           Metallophosphoesterase precursor - Geobacter
           uraniumreducens Rf4
          Length = 759

 Score = 33.5 bits (73), Expect = 2.6
 Identities = 25/82 (30%), Positives = 35/82 (42%), Gaps = 3/82 (3%)
 Frame = +2

Query: 50  PKERYASRKNVSVRSKKREKKD--PEQIEK-VILESVVPGTQTIYVKTWGCAHNNSDSEY 220
           P    A+ K + V S K +  D  P   +  VI E+V    +T+Y+   G A  N   + 
Sbjct: 385 PTNNVATAKQIFVASVKEDDSDETPHVYDPPVIAETVTFPLRTVYMSNAGWAIGNDPDKT 444

Query: 221 MAGLLAANGYKLTEDKWDAQLW 286
              L   NG K  E + D  LW
Sbjct: 445 AVILHTDNGGKTWEVQGDGSLW 466


>UniRef50_A3ZYE3 Cluster: Putative uncharacterized protein; n=2;
           Planctomycetaceae|Rep: Putative uncharacterized protein
           - Blastopirellula marina DSM 3645
          Length = 432

 Score = 33.5 bits (73), Expect = 2.6
 Identities = 21/77 (27%), Positives = 34/77 (44%), Gaps = 2/77 (2%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE--LGQ 355
           T GC  N  ++E +   L   GY+    +  A L ++N+CTV +  +   +  I      
Sbjct: 14  TLGCKVNQYETELVREGLVTAGYRDAITEEPADLCIVNTCTVTNEGDSKSRQVIRRLARD 73

Query: 356 SRGIHVVVAGCVPQGAP 406
           +    +VV GC    AP
Sbjct: 74  NPDARIVVMGCYATRAP 90


>UniRef50_A0M3K8 Cluster: Radical SAM superfamily protein, UPF0004;
           n=20; Bacteroidetes|Rep: Radical SAM superfamily
           protein, UPF0004 - Gramella forsetii (strain KT0803)
          Length = 450

 Score = 33.5 bits (73), Expect = 2.6
 Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 5/81 (6%)
 Frame = +2

Query: 170 IYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEI-- 343
           I V T GC+ N  DSE + G L AN   +  ++ D  + ++N+C     A++   N I  
Sbjct: 11  INVVTLGCSKNVYDSEILMGQLKANDKDVVHEE-DGNIVVINTCGFIDNAKEQSVNTILE 69

Query: 344 --ELGQSRGI-HVVVAGCVPQ 397
             E  Q   +  V V GC+ +
Sbjct: 70  FVEKKQQGDVDKVFVTGCLSE 90


>UniRef50_Q0YRY0 Cluster: MiaB-like tRNA modifying enzyme; n=4;
           Chlorobium/Pelodictyon group|Rep: MiaB-like tRNA
           modifying enzyme - Chlorobium ferrooxidans DSM 13031
          Length = 448

 Score = 33.1 bits (72), Expect = 3.5
 Identities = 19/77 (24%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
 Frame = +2

Query: 182 TWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIE--LGQ 355
           T GC  N +++  +   L + G+K +  +  A+L ++++C V + AE   + +I   +  
Sbjct: 8   TLGCKLNYAETSSILESLCSQGWKQSSIEEGAELIIIHTCAVTAQAEKKCRQKIRGIIRN 67

Query: 356 SRGIHVVVAGCVPQGAP 406
           +    + V GC  Q  P
Sbjct: 68  NPDSRIAVIGCYAQLNP 84


>UniRef50_A4M7N1 Cluster: MiaB-like tRNA modifying enzyme YliG; n=2;
           Thermotogaceae|Rep: MiaB-like tRNA modifying enzyme YliG
           - Petrotoga mobilis SJ95
          Length = 435

 Score = 33.1 bits (72), Expect = 3.5
 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 5/75 (6%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-----TVKSPAEDHFKNEIELG 352
           GC  N++D E   GLL + GYK   +   A    +++C       K   E  F+      
Sbjct: 10  GCPKNDADMEIFKGLLQSKGYKYESNPQLANYIFIDTCGFIEEAKKESIETIFEYVSLKD 69

Query: 353 QSRGIHVVVAGCVPQ 397
            ++ + V+  GC+ Q
Sbjct: 70  NNKNLKVIPIGCLTQ 84


>UniRef50_A3EVU0 Cluster: 2-methylthioadenine synthetase; n=1;
           Leptospirillum sp. Group II UBA|Rep: 2-methylthioadenine
           synthetase - Leptospirillum sp. Group II UBA
          Length = 483

 Score = 33.1 bits (72), Expect = 3.5
 Identities = 16/65 (24%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
 Frame = +2

Query: 164 QTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCT-VKSPAEDHFKNE 340
           +T+ + + GC  N  D+E M   L+  G+++  D  +A++ ++N+C+ V    ++     
Sbjct: 35  KTVGIVSLGCPKNLVDTETMIHSLSEKGFRVIPDLEEAEVIVVNTCSFVTDARKESIDTL 94

Query: 341 IELGQ 355
           +E+ Q
Sbjct: 95  LEMAQ 99


>UniRef50_A0JCT4 Cluster: 5' nucleotidase, putative; n=1;
           Glyptapanteles indiensis|Rep: 5' nucleotidase, putative
           - Glyptapanteles indiensis
          Length = 598

 Score = 33.1 bits (72), Expect = 3.5
 Identities = 27/86 (31%), Positives = 46/86 (53%), Gaps = 2/86 (2%)
 Frame = +2

Query: 77  NVSVRSKKREKKDPEQI-EKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYK 253
           N  V++    +K+ +++ +KVI E++VP       K   C   N  ++ M   ++A  Y 
Sbjct: 341 NDVVKALDNYRKEIQELGKKVIGETLVPLDGPKRCKMHECNSANLLADAMVDYVSALHY- 399

Query: 254 LTEDKW-DAQLWLLNSCTVKSPAEDH 328
           L +DKW DA + ++NS + KS  E H
Sbjct: 400 LEKDKWTDAAVAIVNSGSFKSEHEAH 425


>UniRef50_Q9CKN9 Cluster: UPF0004 protein PM1571; n=239; cellular
           organisms|Rep: UPF0004 protein PM1571 - Pasteurella
           multocida
          Length = 446

 Score = 33.1 bits (72), Expect = 3.5
 Identities = 24/99 (24%), Positives = 46/99 (46%), Gaps = 3/99 (3%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC-TVKSPAEDHFKNEIELGQSRG 364
           GC  N  DSE +   L ++GY +     +A L ++N+C  + S  ++  +   E  +  G
Sbjct: 14  GCPKNLVDSERILTELRSDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENG 73

Query: 365 IHVVVAGCVPQGAPKSGYLHG--LSIVGVQQIDRIVEVV 475
             V+V GC+     +   +H   L + G    + +++ V
Sbjct: 74  -KVIVTGCLGAKEDRIREVHPKVLEVTGPHSYEAVMQQV 111


>UniRef50_UPI000155FF6B Cluster: PREDICTED: similar to lymphocyte
           antigen 6 complex, locus G6E; n=1; Equus caballus|Rep:
           PREDICTED: similar to lymphocyte antigen 6 complex,
           locus G6E - Equus caballus
          Length = 117

 Score = 32.7 bits (71), Expect = 4.6
 Identities = 14/28 (50%), Positives = 16/28 (57%), Gaps = 3/28 (10%)
 Frame = +1

Query: 349 WSESW---HTCCCSRLCTAGRTKKWLPT 423
           WS S+   H CC   LC A  T +WLPT
Sbjct: 69  WSRSYTLQHHCCEQDLCNAATTLQWLPT 96


>UniRef50_A6NW35 Cluster: Putative uncharacterized protein; n=1;
           Bacteroides capillosus ATCC 29799|Rep: Putative
           uncharacterized protein - Bacteroides capillosus ATCC
           29799
          Length = 449

 Score = 32.7 bits (71), Expect = 4.6
 Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 6/76 (7%)
 Frame = +2

Query: 188 GCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSC----TVKSPAEDHFKNEIELGQ 355
           GCA N  ++E M  L    G+++  +   A + +LN+C    + KS A D+     EL +
Sbjct: 18  GCAKNLVNTEQMMALCRDAGHQVVANPEGADVAVLNTCGFIDSAKSEAIDNILELAEL-K 76

Query: 356 SRGI--HVVVAGCVPQ 397
           S+G    ++V GC+ Q
Sbjct: 77  SKGTLGKLLVTGCLSQ 92


>UniRef50_A6R7E1 Cluster: Predicted protein; n=1; Ajellomyces
           capsulatus NAm1|Rep: Predicted protein - Ajellomyces
           capsulatus NAm1
          Length = 513

 Score = 32.7 bits (71), Expect = 4.6
 Identities = 21/62 (33%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
 Frame = +2

Query: 239 ANGYKLTEDKWDA--QLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQGAPKS 412
           +N Y+LTE+KWD+    W  N   + +  ED   N + L     IH V A  +P    KS
Sbjct: 404 SNIYRLTEEKWDSVEARWRENHNQLVTSLEDGKGNPVSLLHKPDIHPVEAIKIPHLDDKS 463

Query: 413 GY 418
            +
Sbjct: 464 KF 465


>UniRef50_Q1FGL7 Cluster: MiaB-like tRNA modifying enzyme; n=5;
           Clostridiales|Rep: MiaB-like tRNA modifying enzyme -
           Clostridium phytofermentans ISDg
          Length = 466

 Score = 32.3 bits (70), Expect = 6.0
 Identities = 20/89 (22%), Positives = 44/89 (49%), Gaps = 2/89 (2%)
 Frame = +2

Query: 152 VPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHF 331
           V G +  ++ T GC  N+ ++E M  L    G  + + +  + ++++N+CTV + A+   
Sbjct: 18  VTGKKVAFL-TLGCKVNSYETEAMQQLFLDAGATIVDFEELSDIYVVNTCTVTNIADRKS 76

Query: 332 KNEIELGQSRGIH--VVVAGCVPQGAPKS 412
           +  +   +    +  V+  GC  Q A ++
Sbjct: 77  RQMLHKAKKNNPNSVVIAVGCYVQAAKEA 105


>UniRef50_Q2U1C1 Cluster: Predicted protein; n=1; Aspergillus
           oryzae|Rep: Predicted protein - Aspergillus oryzae
          Length = 316

 Score = 32.3 bits (70), Expect = 6.0
 Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 6/50 (12%)
 Frame = -3

Query: 175 IYCLSAWYNT-----L*YNLFNLF-GVFFLPLFRSDAYILPTSITFFRSY 44
           I+ + AWYN+     L +++F  + G++F  L  S A ILP SI FF  +
Sbjct: 30  IFVVIAWYNSIELMVLIFSIFKQYKGIYFWSLLISTAGILPYSIGFFMKF 79


>UniRef50_Q8PKB2 Cluster: Putative uncharacterized protein XAC2263;
           n=1; Xanthomonas axonopodis pv. citri|Rep: Putative
           uncharacterized protein XAC2263 - Xanthomonas axonopodis
           pv. citri
          Length = 419

 Score = 31.9 bits (69), Expect = 8.0
 Identities = 14/30 (46%), Positives = 20/30 (66%)
 Frame = +2

Query: 233 LAANGYKLTEDKWDAQLWLLNSCTVKSPAE 322
           L  NG+K+T + + A+L  LNS  +K PAE
Sbjct: 240 LVLNGWKITHEAFTAKLTALNSSCMKHPAE 269


>UniRef50_A0LEL6 Cluster: RNA modification enzyme, MiaB family; n=1;
           Syntrophobacter fumaroxidans MPOB|Rep: RNA modification
           enzyme, MiaB family - Syntrophobacter fumaroxidans
           (strain DSM 10017 / MPOB)
          Length = 440

 Score = 31.9 bits (69), Expect = 8.0
 Identities = 21/81 (25%), Positives = 37/81 (45%), Gaps = 2/81 (2%)
 Frame = +2

Query: 161 TQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNE 340
           ++ + V+T GC  N  +S  M   L    ++    K  A L++++SC V S A    +  
Sbjct: 3   SKKVAVETLGCKVNQYESSVMMESLMQANWQPVSFKGAADLYVVHSCAVTSSAAFQTRQL 62

Query: 341 IELGQ--SRGIHVVVAGCVPQ 397
           +   +  + G  + V GC  Q
Sbjct: 63  LRRARRLNPGALIAVVGCDAQ 83


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 496,923,560
Number of Sequences: 1657284
Number of extensions: 10085444
Number of successful extensions: 25570
Number of sequences better than 10.0: 200
Number of HSP's better than 10.0 without gapping: 24682
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25541
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 28437262108
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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