Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0473
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 208 2e-54
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 208 2e-54
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 208 3e-54
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 202 2e-52
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 200 9e-52
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 51 8e-07
At1g55540.1 68414.m06356 proline-rich family protein contains pr... 30 1.2
At2g36700.1 68415.m04503 pectinesterase family protein contains ... 30 1.6
At2g20780.1 68415.m02442 mannitol transporter, putative similar ... 29 3.8
At2g36960.2 68415.m04533 myb family transcription factor contain... 28 5.0
At2g36960.1 68415.m04532 myb family transcription factor contain... 28 5.0
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 28 5.0
At1g61900.2 68414.m06984 expressed protein contains similarity t... 28 6.6
At1g61900.1 68414.m06983 expressed protein contains similarity t... 28 6.6
At1g51540.1 68414.m05801 kelch repeat-containing protein contain... 28 6.6
At3g32200.1 68416.m04104 hypothetical protein 27 8.7
At1g64385.1 68414.m07297 expressed protein 27 8.7
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 208 bits (509), Expect = 2e-54
Identities = 103/161 (63%), Positives = 125/161 (77%)
Frame = +3
Query: 186 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDAD 365
IGQPL+LL+K +PLV+ L LYDIA V GVAADLSH NTP++V GP EL+ +KD +
Sbjct: 94 IGQPLSLLIKMSPLVSTLHLYDIANVK-GVAADLSHCNTPSQVRDFTGPSELADCLKDVN 152
Query: 366 VVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIASEV 545
VVVIPAGVPRKPGMTRDDLFN NA+IV+ + ++A+N P A + II+NPVNSTVPIA+EV
Sbjct: 153 VVVIPAGVPRKPGMTRDDLFNINANIVKTLVEAVAENCPNAFIHIISNPVNSTVPIAAEV 212
Query: 546 LKKAGVYDPNRVLGVTTLDVVRAATFVGEINGVDPTSVAVP 668
LKK GVYDP ++ GVTTLDVVRA TFV + + V VP
Sbjct: 213 LKKKGVYDPKKLFGVTTLDVVRANTFVSQKKNLKLIDVDVP 253
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 208 bits (509), Expect = 2e-54
Identities = 106/201 (52%), Positives = 145/201 (72%), Gaps = 2/201 (0%)
Frame = +3
Query: 72 MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 245
MF + +A V+ G + F++ S + K IGQPL+LL+K NPLV+ L+L
Sbjct: 1 MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60
Query: 246 YDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLF 425
YDIA TPGVAAD+ H+NT ++VSG+ G ++L A++ AD+V+IPAGVPRKPGMTRDDLF
Sbjct: 61 YDIAN-TPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLF 119
Query: 426 NTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDV 605
N NA IV++++++IA+ P+A+V +I+NPVNSTVPIA+E+ KKAG YD ++ GVTTLDV
Sbjct: 120 NINAGIVKNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDV 179
Query: 606 VRAATFVGEINGVDPTSVAVP 668
VRA TF + V+ V VP
Sbjct: 180 VRARTFYAGKSDVNVAEVNVP 200
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 208 bits (508), Expect = 3e-54
Identities = 104/172 (60%), Positives = 126/172 (73%)
Frame = +3
Query: 153 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGP 332
FK IGQPLA+L+K NPLV+ L LYD+A PGV AD+SHM+T A V G G
Sbjct: 43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANA-PGVTADISHMDTSAVVRGFLGQ 101
Query: 333 EELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNP 512
+L A+ D+V+IPAGVPRKPGMTRDDLFN NA IVR ++ +IA+ PKAIV II+NP
Sbjct: 102 PQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAIVNIISNP 161
Query: 513 VNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFVGEINGVDPTSVAVP 668
VNSTVPIA+EV KKAG +DP +++GVT LDVVRA TFV E+ +DP V VP
Sbjct: 162 VNSTVPIAAEVFKKAGTFDPKKLMGVTMLDVVRANTFVAEVMSLDPREVEVP 213
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 202 bits (492), Expect = 2e-52
Identities = 101/182 (55%), Positives = 132/182 (72%)
Frame = +3
Query: 123 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNT 302
++FS+ S K IGQPLALL+K NPLV+ L+LYDIA TPGVAAD+ H+NT
Sbjct: 20 RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAADVGHINT 78
Query: 303 PAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAP 482
++V G+ G + L+ A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV+++ +IA+ P
Sbjct: 79 RSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCP 138
Query: 483 KAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFVGEINGVDPTSVA 662
A++ +I+NPVNSTVPIA+E+ KKAG+YD ++ GVTTLDVVRA TF V V
Sbjct: 139 HALINMISNPVNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKANVPVAEVN 198
Query: 663 VP 668
VP
Sbjct: 199 VP 200
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 200 bits (487), Expect = 9e-52
Identities = 99/172 (57%), Positives = 122/172 (70%)
Frame = +3
Query: 153 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGP 332
FK IGQ L+LL+K NPLV+ L LYD+ PGV AD+SHM+T A V G G
Sbjct: 43 FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNA-PGVTADVSHMDTGAVVRGFLGA 101
Query: 333 EELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNP 512
++L A+ D+V+IPAG+PRKPGMTRDDLF NA IV+ + +A+ P AIV +I+NP
Sbjct: 102 KQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNP 161
Query: 513 VNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFVGEINGVDPTSVAVP 668
VNSTVPIA+EV KKAG YDP ++LGVTTLDV RA TFV E+ G+DP V VP
Sbjct: 162 VNSTVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGLDPREVDVP 213
>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
similarity to L-lactate dehydrogenase from Lycopersicon
esculentum (GI:1620970, GI:1620972), Hordeum vulgare
(SP|P22988, SP|P22989); contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 353
Score = 50.8 bits (116), Expect = 8e-07
Identities = 46/170 (27%), Positives = 76/170 (44%), Gaps = 7/170 (4%)
Frame = +3
Query: 186 IGQPLALLLKQNPLVTRLALYDIAP-VTPGVAADLSHMNT--PAKVSGHKGPEELSAAIK 356
+G +A + L +AL D P G DL H P E++A
Sbjct: 51 VGMAIAQTILTQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAG-- 108
Query: 357 DADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIA 536
+D+ ++ AG + PG +R +L N ++ R I +A+ +P +I+ I++NPV+ +
Sbjct: 109 -SDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVD----VL 163
Query: 537 SEVLKKAGVYDPNRVLGV-TTLDVVRAATFVG---EINGVDPTSVAVPRH 674
+ V K + NRVLG T LD R + ++N D + V H
Sbjct: 164 TYVAWKLSGFPVNRVLGSGTNLDSSRFRFLIADHLDVNAQDVQAFIVGEH 213
>At1g55540.1 68414.m06356 proline-rich family protein contains
proline rich extensin domain, INTERPRO:IPR002965
Length = 915
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/54 (24%), Positives = 26/54 (48%)
Frame = +3
Query: 378 PAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIAS 539
P P+KP + +T +++ + QN P + + I+NP + P++S
Sbjct: 518 PVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVTPVSS 571
>At2g36700.1 68415.m04503 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 333
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -1
Query: 491 NSFGCILSNGQSNVTD-NGSISVE*VITGHSRFTGYSCWDDYNISIF 354
N+ GCI +NG+ + D G + V ITG +R W Y IF
Sbjct: 217 NTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIF 263
>At2g20780.1 68415.m02442 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 526
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +3
Query: 513 VNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFV 626
+++TV + E+LK+AG+ D ++L T V F+
Sbjct: 316 IDATVYYSPEILKEAGIQDETKLLAATVAVGVTKTVFI 353
>At2g36960.2 68415.m04533 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 741
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 1/43 (2%)
Frame = +2
Query: 203 PSTEAE-SSGDQAGFIRHSACDPRRRSRPFPHEHPSQGQRPQG 328
PS E SSGD+ + H DP PH S G+ P G
Sbjct: 635 PSQLVEASSGDEGSYNPHDDGDPMEEGPADPHTMDSPGKTPCG 677
>At2g36960.1 68415.m04532 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 743
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 1/43 (2%)
Frame = +2
Query: 203 PSTEAE-SSGDQAGFIRHSACDPRRRSRPFPHEHPSQGQRPQG 328
PS E SSGD+ + H DP PH S G+ P G
Sbjct: 637 PSQLVEASSGDEGSYNPHDDGDPMEEGPADPHTMDSPGKTPCG 679
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 28.3 bits (60), Expect = 5.0
Identities = 19/41 (46%), Positives = 20/41 (48%), Gaps = 6/41 (14%)
Frame = +2
Query: 569 PEPRARRHHSG------RSPRCHLRRRDQWRGPDLRRRAPS 673
P P ARRH S RSP RRR P RRR+PS
Sbjct: 353 PSPPARRHRSPPPARRRRSPSPPARRRRSPSPPARRRRSPS 393
>At1g61900.2 68414.m06984 expressed protein contains similarity to
glutamic acid/alanine-rich protein GI:6707830 from
[Trypanosoma congolense]
Length = 413
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -1
Query: 578 AVRVVHSRLLEHFRGNRHCRVNRVC 504
A ++ SR+ E RG +C++NRVC
Sbjct: 238 ATKLDPSRVKETLRGLANCKINRVC 262
>At1g61900.1 68414.m06983 expressed protein contains similarity to
glutamic acid/alanine-rich protein GI:6707830 from
[Trypanosoma congolense]
Length = 433
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -1
Query: 578 AVRVVHSRLLEHFRGNRHCRVNRVC 504
A ++ SR+ E RG +C++NRVC
Sbjct: 238 ATKLDPSRVKETLRGLANCKINRVC 262
>At1g51540.1 68414.m05801 kelch repeat-containing protein contains
Pfam profile PF01344: Kelch motif
Length = 1036
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -1
Query: 419 VITGHSRFTGYSCWDDYNISIFDG 348
++T HS FT WD YN S+ DG
Sbjct: 314 LVTDHSSFTEKEAWDGYNKSL-DG 336
>At3g32200.1 68416.m04104 hypothetical protein
Length = 148
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +2
Query: 545 AQEGGSVRPEPRARRHHSGRSPR 613
A+ G+ RP P R+ H GRSPR
Sbjct: 35 ARSAGAFRPTPH-RKGHGGRSPR 56
>At1g64385.1 68414.m07297 expressed protein
Length = 351
Score = 27.5 bits (58), Expect = 8.7
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 3/66 (4%)
Frame = -1
Query: 662 GDGGRVHAIDLA--DEGGSADYVQSGDAEHAVRVVHSRLLEHFRGNRHCRVNRVC-DDGH 492
GDG ++ D + D+G A + E AV SR + F G C + +C DD H
Sbjct: 72 GDGSKMIGSDSSKSDQGKIASDESDKEEEEAVSKNSSRKKQGFHGEE-CDPSNMCIDDEH 130
Query: 491 NSFGCI 474
C+
Sbjct: 131 EFSACL 136
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,231,489
Number of Sequences: 28952
Number of extensions: 377675
Number of successful extensions: 1084
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1041
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1079
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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