Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0437
(428 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 84 3e-17
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 83 1e-16
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 81 3e-16
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 61 3e-10
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 59 1e-09
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 40 7e-04
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 34 0.036
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 34 0.036
At4g19515.1 68417.m02871 disease resistance family protein simil... 32 0.19
At2g47180.1 68415.m05892 galactinol synthase, putative similar t... 31 0.44
At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) fa... 29 1.8
At4g33200.1 68417.m04727 myosin, putative similar to myosin (GI:... 28 3.1
At1g66270.2 68414.m07524 beta-glucosidase (PSR3.2) nearly identi... 28 3.1
At1g66270.1 68414.m07523 beta-glucosidase (PSR3.2) nearly identi... 28 3.1
At1g60140.1 68414.m06775 glycosyl transferase family 20 protein ... 27 4.1
At5g16720.1 68418.m01958 expressed protein contains Pfam profile... 27 7.1
At5g54690.1 68418.m06811 glycosyl transferase family 8 protein c... 26 9.4
At3g23330.1 68416.m02943 pentatricopeptide (PPR) repeat-containi... 26 9.4
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 84.2 bits (199), Expect = 3e-17
Identities = 37/60 (61%), Positives = 46/60 (76%)
Frame = +2
Query: 248 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLTEGDLLCEIETDKATMGFETPEEGYL 427
S LP H+ + +PALSPTM G+I W KKEGDK+ GD++ EIETDKAT+ FE+ EEGYL
Sbjct: 207 SDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYL 266
Score = 79.8 bits (188), Expect = 7e-16
Identities = 34/50 (68%), Positives = 42/50 (84%)
Frame = +2
Query: 278 LPALSPTMESGSIVSWEKKEGDKLTEGDLLCEIETDKATMGFETPEEGYL 427
+PALSPTM G++V W KKEGDK+ GD+LCEIETDKAT+ FE+ EEG+L
Sbjct: 90 MPALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFL 139
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 82.6 bits (195), Expect = 1e-16
Identities = 35/60 (58%), Positives = 45/60 (75%)
Frame = +2
Query: 248 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLTEGDLLCEIETDKATMGFETPEEGYL 427
S LP H ++ +P+LSPTM G+I W KKEGDK+ G++LCE+ETDKAT+ E EEGYL
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYL 165
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 81.0 bits (191), Expect = 3e-16
Identities = 34/60 (56%), Positives = 45/60 (75%)
Frame = +2
Query: 248 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLTEGDLLCEIETDKATMGFETPEEGYL 427
S LP H ++ +P+LSPTM G+I W KKEGDK+ G++LCE+ETDKAT+ E EEG+L
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFL 165
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 61.3 bits (142), Expect = 3e-10
Identities = 28/53 (52%), Positives = 37/53 (69%)
Frame = +2
Query: 269 KVNLPALSPTMESGSIVSWEKKEGDKLTEGDLLCEIETDKATMGFETPEEGYL 427
++ +PALS TM G IVSW K EGDKL +G+ + +E+DKA M ET +GYL
Sbjct: 41 EIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYL 93
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 58.8 bits (136), Expect = 1e-09
Identities = 27/53 (50%), Positives = 37/53 (69%)
Frame = +2
Query: 269 KVNLPALSPTMESGSIVSWEKKEGDKLTEGDLLCEIETDKATMGFETPEEGYL 427
++ +PALS TM G IVSW K EG+KL +G+ + +E+DKA M ET +GYL
Sbjct: 57 EIFMPALSSTMTEGKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYL 109
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 39.9 bits (89), Expect = 7e-04
Identities = 19/81 (23%), Positives = 43/81 (53%), Gaps = 4/81 (4%)
Frame = +2
Query: 197 NQTVLSTPQWTVQMRYYSSLPSH----IKVNLPALSPTMESGSIVSWEKKEGDKLTEGDL 364
++T+++ Q T R+ S ++ +P + ++ G++ ++ KK GD++ +
Sbjct: 67 SRTIINGYQGTALQRWVRPFSSDSGDVVEAVVPHMGESITDGTLAAFLKKPGDRVEADEA 126
Query: 365 LCEIETDKATMGFETPEEGYL 427
+ +IETDK T+ +P G +
Sbjct: 127 IAQIETDKVTIDIASPASGVI 147
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 34.3 bits (75), Expect = 0.036
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 314 IVSWEKKEGDKLTEGDLLCEIETDKATM 397
++ W KEGD + E LCE+++DKAT+
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATI 119
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 34.3 bits (75), Expect = 0.036
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 314 IVSWEKKEGDKLTEGDLLCEIETDKATM 397
++ W KEGD + E LCE+++DKAT+
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATI 119
>At4g19515.1 68417.m02871 disease resistance family protein similar
to downy mildew resistance protein RPP5 [Arabidopsis
thaliana] GI:6449046; contains Pfam profile PF01582: TIR
domain
Length = 524
Score = 31.9 bits (69), Expect = 0.19
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = +2
Query: 80 RNQILSDGLKKAIRSNITRCISTELAKRKVTNKLLEHAQNQTVL 211
R Q +SD LKK + I CI ++ R + NK+++ + V+
Sbjct: 270 RKQFISDFLKKLVYKGIRICIGDKILSRSLINKVIKESSIAVVV 313
>At2g47180.1 68415.m05892 galactinol synthase, putative similar to
galactinol synthase, isoform GolS-1 GI:5608497 from
[Ajuga reptans]
Length = 344
Score = 30.7 bits (66), Expect = 0.44
Identities = 18/62 (29%), Positives = 26/62 (41%)
Frame = +1
Query: 145 YRTGKKESYE*IVRTCTEPDSSIYTTMDCSDEVLFESTITYKGQPSSSFANHGEWFNCKL 324
Y G + YE I PD +Y MDC E + T YK + + +W +L
Sbjct: 125 YLDGDIQVYENIDHLFDLPDGYLYAVMDCFCEKTWSHTPQYKIRYCQQCPDKVQWPKAEL 184
Query: 325 GK 330
G+
Sbjct: 185 GE 186
>At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 4706
Score = 28.7 bits (61), Expect = 1.8
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 266 IKVNLPALSPTMESGSIVSWEKKEGDKLTEG 358
++V L L P + G IV+W+ K+GDKL G
Sbjct: 4475 VQVQLHPLRPFYK-GEIVAWKIKQGDKLRYG 4504
>At4g33200.1 68417.m04727 myosin, putative similar to myosin
(GI:433663) [Arabidopsis thaliana]
Length = 1522
Score = 27.9 bits (59), Expect = 3.1
Identities = 15/63 (23%), Positives = 26/63 (41%)
Frame = +2
Query: 89 ILSDGLKKAIRSNITRCISTELAKRKVTNKLLEHAQNQTVLSTPQWTVQMRYYSSLPSHI 268
++ D LKK + + CI A R + K + QW +++ SL S +
Sbjct: 1260 LIRDNLKKELSPLLGSCIQAPKASRGIAGKSRSPGGVPQQSPSSQWESILKFLDSLMSRL 1319
Query: 269 KVN 277
+ N
Sbjct: 1320 REN 1322
>At1g66270.2 68414.m07524 beta-glucosidase (PSR3.2) nearly identical
to GI:2286069 from (Arabidopsis thaliana) (Plant Mol.
Biol. 34 (1), 57-68 (1997)); similar to thioglucoside
glucohydrolase (GI:984052) [Arabidopsis thaliana]
Length = 522
Score = 27.9 bits (59), Expect = 3.1
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 230 VQMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLTEGDL 364
V + YY+S S+ P+ + S+VSWE K D G +
Sbjct: 339 VGLNYYTSTFSNYNEKPDPSKPSWKQDSLVSWEPKNVDHSAIGSM 383
>At1g66270.1 68414.m07523 beta-glucosidase (PSR3.2) nearly identical
to GI:2286069 from (Arabidopsis thaliana) (Plant Mol.
Biol. 34 (1), 57-68 (1997)); similar to thioglucoside
glucohydrolase (GI:984052) [Arabidopsis thaliana]
Length = 524
Score = 27.9 bits (59), Expect = 3.1
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 230 VQMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLTEGDL 364
V + YY+S S+ P+ + S+VSWE K D G +
Sbjct: 341 VGLNYYTSTFSNYNEKPDPSKPSWKQDSLVSWEPKNVDHSAIGSM 385
>At1g60140.1 68414.m06775 glycosyl transferase family 20 protein /
trehalose-phosphatase family protein contains Pfam
profile: PF02358 trehalose-phosphatase
Length = 861
Score = 27.5 bits (58), Expect = 4.1
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = +1
Query: 199 PDSSIYTTMDCSDEVLFESTITYKGQPSSSFANHGEWFNCKLGKK 333
PD + D SDE +FE+ T SSS + E F C +G+K
Sbjct: 776 PDFVVCIGDDRSDEEMFENISTTLSAQSSSMST--EIFACTVGRK 818
>At5g16720.1 68418.m01958 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 675
Score = 26.6 bits (56), Expect = 7.1
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +2
Query: 323 WEKKEGDKLTEGDLLCEIETDKATMGFETPEEGY 424
W K GD+ G +L I+ DK GFE E Y
Sbjct: 149 WGKTLGDREDGGLILEMIDDDKFGDGFEIDRESY 182
>At5g54690.1 68418.m06811 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 535
Score = 26.2 bits (55), Expect = 9.4
Identities = 9/19 (47%), Positives = 16/19 (84%)
Frame = +2
Query: 245 YSSLPSHIKVNLPALSPTM 301
Y+SL +HI+++LP L P++
Sbjct: 327 YNSLMNHIRIHLPELFPSL 345
>At3g23330.1 68416.m02943 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 679
Score = 26.2 bits (55), Expect = 9.4
Identities = 11/27 (40%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = +1
Query: 148 RTGK-KESYE*IVRTCTEPDSSIYTTM 225
R GK +E+Y I + C EP S+++T+
Sbjct: 456 RAGKLEEAYNFISKMCVEPTGSVWSTL 482
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,477,853
Number of Sequences: 28952
Number of extensions: 191746
Number of successful extensions: 558
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 558
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 675111616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -