Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Nnor0388
         (530 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    86   2e-17
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    82   2e-16
At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative / arg...    29   2.0  
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot...    28   3.4  
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ...    28   4.5  
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro...    27   6.0  
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro...    27   6.0  
At5g10530.1 68418.m01219 lectin protein kinase, putative similar...    27   6.0  
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g...    27   6.0  
At2g17010.1 68415.m01961 mechanosensitive ion channel domain-con...    27   6.0  
At1g19130.1 68414.m02379 expressed protein                             27   6.0  
At2g21655.1 68415.m02576 expressed protein contains Pfam PF05617...    27   7.9  
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa...    27   7.9  

>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 85.8 bits (203), Expect = 2e-17
 Identities = 56/157 (35%), Positives = 80/157 (50%)
 Frame = +3

Query: 36  MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 215
           MS K EKP L G RIKTRKR+     DP  F DA+VQ      GDL+   K ++S  S L
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57

Query: 216 DYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMR 395
           ++ RYG++ F+V+  GG   PG   S +GE  +    +       + +        K++R
Sbjct: 58  NFTRYGDIFFEVIFIGGRTQPGTVKSDEGE--RHTYSVIDCEPKREAILPSVVYIQKILR 115

Query: 396 RYKYLEKMFEEEMKKVLVYLKGXDPEQRXKLARMTAL 506
           R  +L K  E   ++ L  L+  +  +R KLA  TAL
Sbjct: 116 RKPFLIKNLENVTRRFLQSLELFEENERKKLAIFTAL 152


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 82.2 bits (194), Expect = 2e-16
 Identities = 57/157 (36%), Positives = 79/157 (50%)
 Frame = +3

Query: 36  MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 215
           MS K EKP L G RIKTRKR+     DP  F DALVQ      GDL+   + L+S  S L
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57

Query: 216 DYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMR 395
           ++ RYG++ F+V+  GG    G   S +GE  +    I       + +        K++R
Sbjct: 58  NFSRYGDIFFEVVFIGGRTQTGSVKSDEGE--RHPYSIIDCEPKREAILPSVVYIQKILR 115

Query: 396 RYKYLEKMFEEEMKKVLVYLKGXDPEQRXKLARMTAL 506
           R  +L K  E   ++ L  L+  +  +R KLA  TAL
Sbjct: 116 RKAFLIKNLENVTRRFLQSLELFEENERKKLAIFTAL 152


>At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative /
           arginine--tRNA ligase, putative similar to SP|P37880
           Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA
           ligase) (ArgRS) {Cricetulus longicaudatus}; contains
           Pfam profiles PF00750: arginyl-tRNA synthetase, PF03485:
           arginyl-tRNA synthetase N-terminal domain
          Length = 642

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 14/42 (33%), Positives = 25/42 (59%)
 Frame = +3

Query: 372 QVFVKLMRRYKYLEKMFEEEMKKVLVYLKGXDPEQRXKLARM 497
           QVF K  +    L++ F+E+ ++ +V L+G DP  R   A++
Sbjct: 260 QVFYKASKHKFDLDEAFKEKAQQAVVRLQGGDPVYRKAWAKI 301


>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor [Homo
            sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1706

 Score = 28.3 bits (60), Expect = 3.4
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = +3

Query: 414  KMFEEEMKKVLVYLKGXDPEQRXKLARMTALWI 512
            ++  E  K V +YLK  DP+Q+ +  R   LW+
Sbjct: 1600 RLVTECAKIVKIYLKCTDPQQQEQQQRKPVLWV 1632


>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
           similar to GTL1 [Arabidopsis thaliana] GI:2664198
          Length = 669

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 15/54 (27%), Positives = 25/54 (46%)
 Frame = -2

Query: 391 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSP 230
           +S     S  R  +I SL +K+    +   PS+ ++PP   +PP   T  +  P
Sbjct: 321 MSQERAASASRDAAIISLIQKITGHTIQLPPSLSSQPPPPYQPPPAVTKRVAEP 374


>At5g54650.2 68418.m06805 formin homology 2 domain-containing
           protein / FH2 domain-containing protein contains formin
           homology 2 domain, Pfam:PF02181
          Length = 900

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
 Frame = -2

Query: 310 GDSPSID-TEPPGSSRPPAMSTSNMTSP*RR*SSFDPAES 194
           G SPS   + PP  SR P    +   SP  R SSF P+ S
Sbjct: 143 GSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRS 182


>At5g54650.1 68418.m06804 formin homology 2 domain-containing
           protein / FH2 domain-containing protein contains formin
           homology 2 domain, Pfam:PF02181
          Length = 900

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
 Frame = -2

Query: 310 GDSPSID-TEPPGSSRPPAMSTSNMTSP*RR*SSFDPAES 194
           G SPS   + PP  SR P    +   SP  R SSF P+ S
Sbjct: 143 GSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRS 182


>At5g10530.1 68418.m01219 lectin protein kinase, putative similar to
           receptor lectin kinase 3 [Arabidopsis thaliana]
           gi|4100060|gb|AAD00733; contains protein kinase domain,
           Pfam:PF00069; contains legume lectins alpha and beta
           domains, Pfam:PF00138 and Pfam:PF00139
          Length = 651

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
 Frame = +3

Query: 87  RKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL-DYRRYGEVIFDVLIAG 263
           +++ +K+K +      ++ + LER  G     YK L SA +   D R+ GE  F  +  G
Sbjct: 293 KRKQQKKKAEETENLTSINEDLERGAGPRKFTYKDLASAANNFADDRKLGEGGFGAVYRG 352

Query: 264 GL 269
            L
Sbjct: 353 YL 354


>At4g25000.1 68417.m03587 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative similar to
           alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
           GI:21670851; contains Pfam profile PF00128: Alpha
           amylase, catalytic domain
          Length = 423

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 13/32 (40%), Positives = 17/32 (53%)
 Frame = +3

Query: 96  DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 191
           D K  YD N  R  L Q +E AGG +  A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269


>At2g17010.1 68415.m01961 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 779

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 16/60 (26%), Positives = 26/60 (43%)
 Frame = +3

Query: 198 SAGSKLDYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQV 377
           +A +  D  R   V F+V  AGG  + G   S    S  +++     N+D   ++  E V
Sbjct: 165 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEEEVV 224


>At1g19130.1 68414.m02379 expressed protein
          Length = 187

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 15/51 (29%), Positives = 24/51 (47%)
 Frame = +3

Query: 270 LLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 422
           LLP GSVS     P   T  F   E +  +R+     ++     ++L++MF
Sbjct: 59  LLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTSGLVRFLQRMF 109


>At2g21655.1 68415.m02576 expressed protein contains Pfam PF05617:
           Arabidopsis thaliana protein of unknown function
           (DUF784)
          Length = 156

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 14/46 (30%), Positives = 23/46 (50%)
 Frame = -2

Query: 427 SSNIFSKYLYRRISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSID 290
           + N+ SKY  ++I++   CSK   V    + E M  ++   S S D
Sbjct: 55  NENVGSKYSKKQINYLMNCSKKMAVPDKCIEEVMAEIIQNKSASRD 100


>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
           profiles: PF04096 nucleoporin autopeptidase, PF03093
           nucleoporin FG repeat family
          Length = 1041

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 24/75 (32%), Positives = 27/75 (36%)
 Frame = -2

Query: 469 GSKPFK*TRTFFISSSNIFSKYLYRRISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSID 290
           GS PF  T     SSSN+F        S   + S F   + S L         G S SI 
Sbjct: 467 GSSPFGVT-----SSSNLFGSGSSTTTSVFGSSSAFGTTTPSPLFGSSSTPGFGSSSSIF 521

Query: 289 TEPPGSSRPPAMSTS 245
              PG    PA   S
Sbjct: 522 GSAPGQGATPAFGNS 536


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,214,206
Number of Sequences: 28952
Number of extensions: 273385
Number of successful extensions: 1036
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 958
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1032
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 987020800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)