BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Nnor0377 (596 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 53 3e-08 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 35 0.010 SPCC1223.15c |spc19||DASH complex subunit Spc19|Schizosaccharomy... 30 0.22 SPAC19A8.07c |||U3 snoRNP-associated protein Imp4 |Schizosacchar... 29 0.39 SPAC7D4.11c |sec39||secretory pathway protein Sec39 |Schizosacch... 28 1.2 SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosac... 28 1.2 SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 27 2.1 SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase Cgs2|Schi... 26 3.6 SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 26 4.8 SPCC10H11.02 |cwf23|SPCP31B10.01|DNAJ domain protein Cwf23|Schiz... 26 4.8 SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 26 4.8 SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr 1|||M... 25 8.4 SPBC1709.19c |||NifU-like protein|Schizosaccharomyces pombe|chr ... 25 8.4 SPAC806.04c |||DUF89 family protein|Schizosaccharomyces pombe|ch... 25 8.4 >SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 53.2 bits (122), Expect = 3e-08 Identities = 38/132 (28%), Positives = 62/132 (46%), Gaps = 1/132 (0%) Frame = +2 Query: 188 YLKHLMVEHENLLSKMKKSSEEARRLQEIKPIVNVLEQRIALYDSIESLKELNKKEGDDS 367 YL+ V +++ S M ++ A+R+ ++ + N + +L + I LK++ +E D + Sbjct: 48 YLQFHQVNNKDQ-SAMTNDTDLAKRIARLRRVHNAYSKFKSLSEQISDLKQMEAQESD-A 105 Query: 368 EIKKM-IKEEAAIYLKRXXXXXXXXXXXXXXXXXTEGGVLLELTAGAGGQEAMLFAKELL 544 E+K M + E I K L+E+ G GG EA +FA EL+ Sbjct: 106 EVKMMAVTEINEISNKIPKSIEDLENTLLPQADSYALPALIEIRPGVGGTEAAIFANELV 165 Query: 545 NMYQSYAINKGW 580 MY YA KGW Sbjct: 166 EMYFQYANFKGW 177 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 34.7 bits (76), Expect = 0.010 Identities = 22/64 (34%), Positives = 39/64 (60%), Gaps = 9/64 (14%) Frame = +2 Query: 215 ENLLSKMK-KSSEEARRLQEIKPIVN-------VLEQRI-ALYDSIESLKELNKKEGDDS 367 EN++S +K K SEE+ RLQE++ +N L +RI A D + S++ +NK + ++ Sbjct: 798 ENIISDLKNKLSEESTRLQELQSQLNQDKNQIETLNERISAAADELSSMESINKNQANEL 857 Query: 368 EIKK 379 ++ K Sbjct: 858 KLAK 861 >SPCC1223.15c |spc19||DASH complex subunit Spc19|Schizosaccharomyces pombe|chr 3|||Manual Length = 152 Score = 30.3 bits (65), Expect = 0.22 Identities = 19/51 (37%), Positives = 33/51 (64%), Gaps = 2/51 (3%) Frame = +2 Query: 191 LKHLMV--EHENLLSKMKKSSEEARRLQEIKPIVNVLEQRIALYDSIESLK 337 +KH++ H NL+S+ + S+ +A+ + +KPI++ QR L DSI SL+ Sbjct: 38 IKHILKVQRHFNLISEDELSARQAKFEEIVKPILSKAFQR--LEDSISSLQ 86 >SPAC19A8.07c |||U3 snoRNP-associated protein Imp4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 289 Score = 29.5 bits (63), Expect = 0.39 Identities = 17/44 (38%), Positives = 28/44 (63%) Frame = +2 Query: 257 RRLQEIKPIVNVLEQRIALYDSIESLKELNKKEGDDSEIKKMIK 388 +R QE++ + E+R AL ++E KELNK +DS+++K K Sbjct: 15 KRNQELQE-AKLNEKRRALRKALEGNKELNKDLQEDSQLQKDYK 57 >SPAC7D4.11c |sec39||secretory pathway protein Sec39 |Schizosaccharomyces pombe|chr 1|||Manual Length = 769 Score = 27.9 bits (59), Expect = 1.2 Identities = 15/67 (22%), Positives = 35/67 (52%) Frame = +2 Query: 158 MNLSDPAIQTYLKHLMVEHENLLSKMKKSSEEARRLQEIKPIVNVLEQRIALYDSIESLK 337 +N+S+ AI M++ ++ L+ + +E L++I+P V + + + Y++ + Sbjct: 410 LNISEDAISVVFLKQMLQEKSFLALKQMRNEIG--LKDIEPKVLIDCLKTSFYENFRAAS 467 Query: 338 ELNKKEG 358 +NK G Sbjct: 468 NMNKNRG 474 >SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosaccharomyces pombe|chr 3|||Manual Length = 305 Score = 27.9 bits (59), Expect = 1.2 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 1/67 (1%) Frame = +2 Query: 200 LMVEHENLLSKMKKSSEEARRLQEIKPIVNVLEQRIALYDSIESLKELN-KKEGDDSEIK 376 L++ + K+K+ EEARR +E + V + Q+ +E K + + DD E Sbjct: 167 LVLARKEAKRKLKEKEEEARRQKEQQQGVVQVRQQKEYLKELERQKTVPLARYEDDPEYN 226 Query: 377 KMIKEEA 397 K +KE + Sbjct: 227 KELKERS 233 >SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase Gpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1448 Score = 27.1 bits (57), Expect = 2.1 Identities = 15/54 (27%), Positives = 32/54 (59%) Frame = +2 Query: 122 LLHFRRHCSHNIMNLSDPAIQTYLKHLMVEHENLLSKMKKSSEEARRLQEIKPI 283 LL+ R CS ++ +L+ ++ ++ +E++N LSK+ + +RRL+ + I Sbjct: 790 LLNGRMICSFSVDSLNTADLKMLMQ---MEYDNYLSKLSNIAGSSRRLKNSRAI 840 >SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase Cgs2|Schizosaccharomyces pombe|chr 3|||Manual Length = 346 Score = 26.2 bits (55), Expect = 3.6 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +1 Query: 493 NCRSWRARSDVICKRTLEHVPVLC 564 N WRA S +I +R L H+ + C Sbjct: 234 NIHIWRACSSLIAQRKLSHILIEC 257 >SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces pombe|chr 3|||Manual Length = 1088 Score = 25.8 bits (54), Expect = 4.8 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = +2 Query: 122 LLHFRRHCSHNIMNLSDPAIQTYLKHLMVEHENL 223 L+ FR HCS + +L A+ KH+ + E++ Sbjct: 699 LVRFRNHCSDYLSDLCRTAVLKEYKHVNEDTEDV 732 >SPCC10H11.02 |cwf23|SPCP31B10.01|DNAJ domain protein Cwf23|Schizosaccharomyces pombe|chr 3|||Manual Length = 289 Score = 25.8 bits (54), Expect = 4.8 Identities = 16/69 (23%), Positives = 34/69 (49%) Frame = +2 Query: 209 EHENLLSKMKKSSEEARRLQEIKPIVNVLEQRIALYDSIESLKELNKKEGDDSEIKKMIK 388 + E +K EEA Q + ++ E+ YDS+E K+ N+++ +++ + + Sbjct: 71 DSERFAKLARKRREEAFNFQRKSMVDDLRERERQFYDSLE--KKENERDRLQEKLRALQE 128 Query: 389 EEAAIYLKR 415 E A + +R Sbjct: 129 ESANLRRQR 137 >SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 345 Score = 25.8 bits (54), Expect = 4.8 Identities = 15/60 (25%), Positives = 31/60 (51%) Frame = +2 Query: 77 PKLLFLRSFLNSERQLLHFRRHCSHNIMNLSDPAIQTYLKHLMVEHENLLSKMKKSSEEA 256 PK+ + ++E + H++RH + + D +IQ+YL E E L ++ +++A Sbjct: 254 PKIASANAGSSNEISIPHYQRHKKSHKGAIQDSSIQSYLD----EEEKLSRELDHKTKDA 309 >SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr 1|||Manual Length = 468 Score = 25.0 bits (52), Expect = 8.4 Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 1/66 (1%) Frame = +2 Query: 209 EHENLLSKMKKSSEEARRLQEIKP-IVNVLEQRIALYDSIESLKELNKKEGDDSEIKKMI 385 EH+ L ++ + E++ + N+ + A+ S ++L +NK + D +KK + Sbjct: 34 EHKEKLGAIRGGGSLQEKNAELRAELDNIRNAQAAIRSSKQTL--INKVKAQDELLKKKV 91 Query: 386 KEEAAI 403 KE A+ Sbjct: 92 KELTAM 97 >SPBC1709.19c |||NifU-like protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 260 Score = 25.0 bits (52), Expect = 8.4 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +2 Query: 137 RHCSHNIMNLSDPAIQTYLKHLMVEHENLLSKMKKSSEEA 256 R CS + + L + IQ LKH + E EN++ + E A Sbjct: 201 RTCSSSAVTLKN-GIQQMLKHYIPEVENVVQVLDPEEEVA 239 >SPAC806.04c |||DUF89 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 440 Score = 25.0 bits (52), Expect = 8.4 Identities = 16/56 (28%), Positives = 32/56 (57%) Frame = +2 Query: 224 LSKMKKSSEEARRLQEIKPIVNVLEQRIALYDSIESLKELNKKEGDDSEIKKMIKE 391 LSK+ +S+++ + + KP++N LE A I++ + L EG+ +I + +E Sbjct: 43 LSKLASTSKDSEVVAQGKPLLNDLE---AFKSDIKNDRPLVPLEGEGQDIVEYNEE 95 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,987,967 Number of Sequences: 5004 Number of extensions: 33322 Number of successful extensions: 141 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 141 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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