Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0322
(354 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g50920.1 68414.m05725 GTP-binding protein-related similar to ... 192 8e-50
At1g10300.1 68414.m01160 GTP-binding protein-related contains si... 171 2e-43
At3g23860.1 68416.m02999 GTP-binding protein-related low similar... 58 1e-09
At3g10830.1 68416.m01304 hypothetical protein 39 0.001
At1g80770.1 68414.m09476 expressed protein 36 0.010
At5g24430.1 68418.m02879 calcium-dependent protein kinase, putat... 27 3.6
At5g45520.1 68418.m05591 hypothetical protein 27 4.7
At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 26 6.3
At2g30770.1 68415.m03752 cytochrome P450 71A13, putative (CYP71A... 26 6.3
At5g56560.1 68418.m07058 F-box family protein contains F-box dom... 26 8.3
At3g51450.1 68416.m05635 strictosidine synthase family protein s... 26 8.3
>At1g50920.1 68414.m05725 GTP-binding protein-related similar to
GTP-binding protein SP:Q99ME9 from [Mus musculus]
Length = 671
Score = 192 bits (467), Expect = 8e-50
Identities = 83/117 (70%), Positives = 103/117 (88%)
Frame = +2
Query: 2 NFKKIAVVPTAKDFIDIILSKTQRKTPTVVHKHYKISRIRGFYIRKVKYTQQNFHDRLSR 181
NFK+I VVP K+F+DIILS+TQR+TPTVVHK YKI+R+R FY+RKVKYTQ NFH +LS
Sbjct: 5 NFKRITVVPNGKEFVDIILSRTQRQTPTVVHKGYKINRLRQFYMRKVKYTQTNFHAKLSA 64
Query: 182 IIQEFPKLDDVHPFYADLMNVLYDKDHYKLGLGQLNTARHLIDNVAKDYVRLLKYGD 352
II EFP+L+ +HPFY DL++VLY+KDHYKL LGQ+NTAR+LI ++KDYV+LLKYGD
Sbjct: 65 IIDEFPRLEQIHPFYGDLLHVLYNKDHYKLALGQVNTARNLISKISKDYVKLLKYGD 121
>At1g10300.1 68414.m01160 GTP-binding protein-related contains
similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4
from [Homo sapiens];
Length = 687
Score = 171 bits (415), Expect = 2e-43
Identities = 73/117 (62%), Positives = 98/117 (83%)
Frame = +2
Query: 2 NFKKIAVVPTAKDFIDIILSKTQRKTPTVVHKHYKISRIRGFYIRKVKYTQQNFHDRLSR 181
NFKKI VVP K F+DI+LS+TQR+TPTVVHK +I ++R FY+RKVK+T+ NF+++LS
Sbjct: 34 NFKKITVVPNGKQFVDIVLSRTQRQTPTVVHKGDRICKLRSFYMRKVKFTESNFNEKLSA 93
Query: 182 IIQEFPKLDDVHPFYADLMNVLYDKDHYKLGLGQLNTARHLIDNVAKDYVRLLKYGD 352
II EFP+L ++ PFY DL++VLY+KDHYKL LGQ+NTA++ I +A DYV+LLK+GD
Sbjct: 94 IIDEFPRLKEIQPFYEDLLHVLYNKDHYKLALGQVNTAKNKISKIAMDYVKLLKHGD 150
>At3g23860.1 68416.m02999 GTP-binding protein-related low similarity
to SP|Q99ME9 Nucleolar GTP-binding protein 1 (Chronic
renal failure gene protein) {Mus musculus}
Length = 230
Score = 58.4 bits (135), Expect = 1e-09
Identities = 40/120 (33%), Positives = 63/120 (52%), Gaps = 4/120 (3%)
Frame = +2
Query: 5 FKKI-AVVPTAKDFIDIILSKTQRKTPTVVHK---HYKISRIRGFYIRKVKYTQQNFHDR 172
FKKI AVVPT DF I + Q T++ H IS IR Y KV ++
Sbjct: 6 FKKISAVVPTELDFDRAIRFEYQIPNCTLIPDRVCHDDISDIRQKYAVKVMSAGTTLSNK 65
Query: 173 LSRIIQEFPKLDDVHPFYADLMNVLYDKDHYKLGLGQLNTARHLIDNVAKDYVRLLKYGD 352
L+ ++ EFP + + P YA L++ Y+ HY + Q++ + L++ ++ +YV LL+ D
Sbjct: 66 LNDVLHEFPCVRHLDPVYASLLHQRYNMYHYDRAVRQVSVTQTLVNVMSFNYVDLLRKDD 125
>At3g10830.1 68416.m01304 hypothetical protein
Length = 147
Score = 38.7 bits (86), Expect = 0.001
Identities = 27/100 (27%), Positives = 46/100 (46%), Gaps = 6/100 (6%)
Frame = +2
Query: 5 FKKIAVVPTAKDFIDIILSKTQRKTPTVVHKHY------KISRIRGFYIRKVKYTQQNFH 166
FKK++V+ T KDF+D I+S + + + + K I + V+ + F
Sbjct: 27 FKKMSVLLTEKDFVDAIVSVSLKDLTRSLFVRFEAKACPKFDLIHKTSVETVRCVGKTFM 86
Query: 167 DRLSRIIQEFPKLDDVHPFYADLMNVLYDKDHYKLGLGQL 286
LS++ +EFP + HP L+ +D H L Q+
Sbjct: 87 HMLSKVSREFPPRNRTHP--GGLLYQRFDPTHLSLARQQV 124
>At1g80770.1 68414.m09476 expressed protein
Length = 451
Score = 35.5 bits (78), Expect = 0.010
Identities = 26/112 (23%), Positives = 53/112 (47%), Gaps = 1/112 (0%)
Frame = +2
Query: 5 FKKIAVVPTAKDFIDIILSKTQRKTPTV-VHKHYKISRIRGFYIRKVKYTQQNFHDRLSR 181
F+K+ +V + D L K++R PT + K R RG +++ + L
Sbjct: 92 FQKLPMVMPSIDLYASALRKSKRVQPTKGIANIAKRERNRG--AKQLDAFMKELALPLKG 149
Query: 182 IIQEFPKLDDVHPFYADLMNVLYDKDHYKLGLGQLNTARHLIDNVAKDYVRL 337
++ FP+ +HP+ L+++ Y+ LG+++ R + +V K++ L
Sbjct: 150 YMESFPRKKLLHPYERSLIDLTLGDGKYEEVLGKVDVLRKKVQSVGKEHASL 201
>At5g24430.1 68418.m02879 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium/calmodulin-dependent
protein kinase CaMK1 [Nicotiana tabacum]
gi|16904222|gb|AAL30818
Length = 594
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2
Query: 17 AVVPTAKDFIDIILSKTQRKTPT 85
++ PTAKDF+ +L+K RK T
Sbjct: 373 SISPTAKDFVKRLLNKDHRKRMT 395
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 26.6 bits (56), Expect = 4.7
Identities = 10/27 (37%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
Frame = -3
Query: 286 QLSESKF-VMILVVQYVHEISIKWMDI 209
+++E K+ V IL ++Y+HE ++W D+
Sbjct: 1106 RITEDKWDVEILRLKYLHEFKVEWRDL 1132
>At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q64350
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 730
Score = 26.2 bits (55), Expect = 6.3
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +2
Query: 122 GFYIRKVKYTQQNFHDRLSRIIQEFPKLDDVHPFYADLMNVLYDKD 259
GFY +K+ Q + + QE K ++ P + ++++LYDKD
Sbjct: 633 GFYAKKID-EQIEVIMKFEEMCQESHK--ELGPLFTQILHLLYDKD 675
>At2g30770.1 68415.m03752 cytochrome P450 71A13, putative (CYP71A13)
Identical to Cytochrome P450 71A13 (SP:O49342)
[Arabidopsis thaliana]; similar to Cytochrome P450
(gi:5713172) [Nicotiana tabacum].
Length = 503
Score = 26.2 bits (55), Expect = 6.3
Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 107 ISRIRGFYIRKVKYTQQNFHDRLSRIIQE-FPKLDDVHPFYADLMNVLYDKD 259
I IRGF K+K + F D + +++QE +D F L+++ DK+
Sbjct: 234 IDGIRGFN-NKIKEVSRGFSDLMDKVVQEHLEASNDKADFVDILLSIEKDKN 284
>At5g56560.1 68418.m07058 F-box family protein contains F-box domain
Pfam:PF00646
Length = 607
Score = 25.8 bits (54), Expect = 8.3
Identities = 19/62 (30%), Positives = 29/62 (46%)
Frame = +2
Query: 119 RGFYIRKVKYTQQNFHDRLSRIIQEFPKLDDVHPFYADLMNVLYDKDHYKLGLGQLNTAR 298
+GF I+ T NF D LS + F + +V AD+ + D K +G L + +
Sbjct: 409 KGFVIKAPSLTDLNFEDTLSNFLM-FEPMPEV--IKADIQVIC---DQSKNFIGSLTSIQ 462
Query: 299 HL 304
HL
Sbjct: 463 HL 464
>At3g51450.1 68416.m05635 strictosidine synthase family protein
similar to hemomucin [Drosophila
melanogaster][GI:1280434], strictosidine synthase
[Rauvolfia serpentina][SP|P15324]
Length = 371
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -3
Query: 334 SHIVFCDIVNKMSGCIQLSESKFVMILVVQY 242
SHI CD + K C LS+S + + +++Y
Sbjct: 334 SHIATCDKIGKYLYCGSLSQSHILRLDLLKY 364
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,600,887
Number of Sequences: 28952
Number of extensions: 146860
Number of successful extensions: 342
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 338
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 341
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 449370720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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