Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0246
(497 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 132 1e-31
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 132 1e-31
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 121 2e-28
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 121 3e-28
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 118 2e-27
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 118 2e-27
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 118 2e-27
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 104 3e-23
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 104 3e-23
At4g32350.1 68417.m04605 expressed protein contains Pfam profile... 31 0.33
At1g67230.1 68414.m07652 expressed protein 28 4.0
At4g02460.1 68417.m00333 DNA mismatch repair protein, putative s... 27 5.3
At5g65910.1 68418.m08296 BSD domain-containing protein contains ... 27 7.0
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 132 bits (320), Expect = 1e-31
Identities = 64/117 (54%), Positives = 88/117 (75%), Gaps = 1/117 (0%)
Frame = +3
Query: 3 GTADFLSKMQDGEKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDAN 182
GT+ F+ KMQ ++ ++N +IGQFGVGFYSA+LVAD + V SKHNDD Q+VW+S AN
Sbjct: 175 GTSAFVEKMQ-----SSGDLN-LIGQFGVGFYSAYLVADYIEVISKHNDDSQYVWESKAN 228
Query: 183 S-FSVAEDPRGDTLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLWAS 350
F+V+ED + L RGT + LH+++EA +YL+ ++ LVK+YS+FINFPI LWAS
Sbjct: 229 GKFAVSEDTWNEPLGRGTEIRLHLRDEAGEYLEESKLKELVKRYSEFINFPISLWAS 285
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 132 bits (320), Expect = 1e-31
Identities = 64/117 (54%), Positives = 88/117 (75%), Gaps = 1/117 (0%)
Frame = +3
Query: 3 GTADFLSKMQDGEKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDAN 182
GT+ F+ KMQ ++ ++N +IGQFGVGFYSA+LVAD + V SKHNDD Q+VW+S AN
Sbjct: 175 GTSAFVEKMQ-----SSGDLN-LIGQFGVGFYSAYLVADYIEVISKHNDDSQYVWESKAN 228
Query: 183 S-FSVAEDPRGDTLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLWAS 350
F+V+ED + L RGT + LH+++EA +YL+ ++ LVK+YS+FINFPI LWAS
Sbjct: 229 GKFAVSEDTWNEPLGRGTEIRLHLRDEAGEYLEESKLKELVKRYSEFINFPISLWAS 285
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 121 bits (292), Expect = 2e-28
Identities = 58/115 (50%), Positives = 81/115 (70%), Gaps = 1/115 (0%)
Frame = +3
Query: 3 GTADFLSKMQDGEKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA- 179
GT +F+ +Q G + MIGQFGVGFYSA+LVA+ V V +KHNDD Q+VW+S A
Sbjct: 106 GTKEFMEALQAGADVS------MIGQFGVGFYSAYLVAEKVVVTTKHNDDEQYVWESQAG 159
Query: 180 NSFSVAEDPRGDTLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW 344
SF+V D G+ L RGT +TL +K++ +YL+ ++ LVKK+S+FI++PIYLW
Sbjct: 160 GSFTVTRDVDGEPLGRGTKITLFLKDDQLEYLEERRLKDLVKKHSEFISYPIYLW 214
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 121 bits (291), Expect = 3e-28
Identities = 57/103 (55%), Positives = 75/103 (72%), Gaps = 1/103 (0%)
Frame = +3
Query: 39 EKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGD 215
E AA MIGQFGVGFYSA+LVAD V V +KHNDD Q+VW+S A SF+V D G+
Sbjct: 107 EALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGE 166
Query: 216 TLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW 344
TL RGT + L++KE+ +YL+ ++ LVKK+S+FI++PI LW
Sbjct: 167 TLGRGTKMVLYLKEDQLEYLEERRLKDLVKKHSEFISYPISLW 209
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 118 bits (285), Expect = 2e-27
Identities = 55/103 (53%), Positives = 74/103 (71%), Gaps = 1/103 (0%)
Frame = +3
Query: 39 EKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGD 215
E AA MIGQFGVGFYSA+LVAD V V +KHNDD Q+VW+S A SF+V D G+
Sbjct: 107 EALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGE 166
Query: 216 TLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW 344
L RGT + L++KE+ +Y++ ++ LVKK+S+FI++PI LW
Sbjct: 167 ALGRGTKMVLYLKEDQMEYIEERRLKDLVKKHSEFISYPISLW 209
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 118 bits (285), Expect = 2e-27
Identities = 58/118 (49%), Positives = 83/118 (70%), Gaps = 4/118 (3%)
Frame = +3
Query: 3 GTADFLSKMQDGEKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKH-NDDRQHVWQSDA 179
GTA F+ ++D + + N++IGQFGVGFYSAFLVAD V V++K D+Q+VW+ +A
Sbjct: 191 GTAKFMKALKDSKDAGGD--NNLIGQFGVGFYSAFLVADRVIVSTKSPKSDKQYVWEGEA 248
Query: 180 NS--FSVAEDPRGDTL-KRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW 344
NS F++ ED +L RGT +TLH+K+EA ++ + I+ LVK YSQF++FPIY W
Sbjct: 249 NSSSFTIQEDTDPQSLIPRGTRITLHLKQEAKNFADPERIQKLVKNYSQFVSFPIYTW 306
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 118 bits (284), Expect = 2e-27
Identities = 55/103 (53%), Positives = 74/103 (71%), Gaps = 1/103 (0%)
Frame = +3
Query: 39 EKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGD 215
E AA MIGQFGVGFYSA+LVAD V V +KHNDD Q+VW+S A SF+V D G+
Sbjct: 107 EALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGE 166
Query: 216 TLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW 344
L RGT + L++KE+ +Y++ ++ LVKK+S+FI++PI LW
Sbjct: 167 ALGRGTKMILYLKEDQMEYIEERRLKDLVKKHSEFISYPISLW 209
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 104 bits (250), Expect = 3e-23
Identities = 53/119 (44%), Positives = 82/119 (68%), Gaps = 5/119 (4%)
Frame = +3
Query: 3 GTADFLSKMQDGEKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKH-NDDRQHVWQS-- 173
GT+ FL +++ + A N +IGQFGVGFYSAFLVA+ V V++K D+Q+VW+S
Sbjct: 173 GTSKFLKALKENKDLGAD--NGLIGQFGVGFYSAFLVAEKVVVSTKSPKSDKQYVWESVA 230
Query: 174 DANSFSVAEDPRGDT-LKRGTHLTLHMKEEAS-DYLQADTIRALVKKYSQFINFPIYLW 344
D++S+ + E+ D L+RGT +TL+++E+ ++ ++ I+ LVK YSQF+ FPIY W
Sbjct: 231 DSSSYLIREETDPDNILRRGTQITLYLREDDKYEFAESTRIKNLVKNYSQFVGFPIYTW 289
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 104 bits (250), Expect = 3e-23
Identities = 53/119 (44%), Positives = 82/119 (68%), Gaps = 5/119 (4%)
Frame = +3
Query: 3 GTADFLSKMQDGEKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKH-NDDRQHVWQS-- 173
GT+ FL +++ + A N +IGQFGVGFYSAFLVA+ V V++K D+Q+VW+S
Sbjct: 173 GTSKFLKALKENKDLGAD--NGLIGQFGVGFYSAFLVAEKVVVSTKSPKSDKQYVWESVA 230
Query: 174 DANSFSVAEDPRGDT-LKRGTHLTLHMKEEAS-DYLQADTIRALVKKYSQFINFPIYLW 344
D++S+ + E+ D L+RGT +TL+++E+ ++ ++ I+ LVK YSQF+ FPIY W
Sbjct: 231 DSSSYLIREETDPDNILRRGTQITLYLREDDKYEFAESTRIKNLVKNYSQFVGFPIYTW 289
>At4g32350.1 68417.m04605 expressed protein contains Pfam profile:
PF03398 eukaryotic protein of unknown function, DUF292
Length = 732
Score = 31.5 bits (68), Expect = 0.33
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +3
Query: 114 ADSVTVASKHNDDRQHVWQSDANSFSVAEDPRGDTLKRGTH 236
A+S+ S+H++DR+ + S EDP GD +K G +
Sbjct: 453 AESINPGSRHHNDRESKENAVLVGKSTEEDPSGDNVKGGEY 493
>At1g67230.1 68414.m07652 expressed protein
Length = 1132
Score = 27.9 bits (59), Expect = 4.0
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -1
Query: 341 QVNGEVDELRVLLDERADGVRLQ 273
++N E DELRV +ER++ +RLQ
Sbjct: 468 EINKEKDELRVTEEERSEYLRLQ 490
>At4g02460.1 68417.m00333 DNA mismatch repair protein, putative
similar to SP|P54278 PMS1 protein homolog 2 (DNA
mismatch repair protein PMS2) {Homo sapiens}; contains
Pfam profiles PF02518: ATPase, histidine kinase-, DNA
gyrase B-, and HSP90-like domain protein, PF01119: DNA
mismatch repair protein, C-terminal domain
Length = 923
Score = 27.5 bits (58), Expect = 5.3
Identities = 21/75 (28%), Positives = 37/75 (49%)
Frame = +3
Query: 69 MIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDANSFSVAEDPRGDTLKRGTHLTLH 248
++GQF +GF A L D + + +H D + ++ A S + + P L + +L L
Sbjct: 707 VLGQFNLGFIIAKLERD-LFIVDQHAADEKFNFEHLARSTVLNQQP----LLQPLNLELS 761
Query: 249 MKEEASDYLQADTIR 293
+EE + + D IR
Sbjct: 762 PEEEVTVLMHMDIIR 776
>At5g65910.1 68418.m08296 BSD domain-containing protein contains
Pfam profile PF03909: BSD domain
Length = 432
Score = 27.1 bits (57), Expect = 7.0
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = +3
Query: 111 VADSVTVASKHNDDRQHVWQSDANSFSVAEDPRG 212
+A+S+ + + DD +H+ Q + S S ++ PRG
Sbjct: 8 IANSLKLDEEDEDDDKHLNQQPSESVSDSQSPRG 41
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,772,529
Number of Sequences: 28952
Number of extensions: 77411
Number of successful extensions: 315
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 295
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 303
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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