BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Nnor0232 (398 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 89 1e-18 At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 85 2e-17 At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 84 4e-17 At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 81 3e-16 At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 80 5e-16 At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 31 0.21 At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 29 1.1 At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 2.0 At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 28 2.6 At4g37390.1 68417.m05294 auxin-responsive GH3 family protein sim... 27 3.5 At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma m... 27 4.6 At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma m... 27 6.1 At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma m... 27 6.1 At1g23550.1 68414.m02962 expressed protein 27 6.1 At1g12940.1 68414.m01503 high-affinity nitrate transporter, puta... 27 6.1 At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,... 26 8.0 At4g04090.1 68417.m00578 speckle-type POZ protein-related contai... 26 8.0 >At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis thaliana] Length = 134 Score = 88.6 bits (210), Expect = 1e-18 Identities = 49/122 (40%), Positives = 68/122 (55%), Gaps = 8/122 (6%) Frame = +1 Query: 55 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 213 MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ F+ Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60 Query: 214 SLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 390 L G+ +AG +Y+ + G + +IR K G G+ KT Q++V LYEEP+ P Q Sbjct: 61 GHLAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNM 120 Query: 391 VV 396 VV Sbjct: 121 VV 122 >At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis thaliana] Length = 134 Score = 85.0 bits (201), Expect = 2e-17 Identities = 48/122 (39%), Positives = 66/122 (54%), Gaps = 8/122 (6%) Frame = +1 Query: 55 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 213 MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E + I+ F+ Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60 Query: 214 SLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 390 L G+ +AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q Sbjct: 61 GHLAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNM 120 Query: 391 VV 396 VV Sbjct: 121 VV 122 >At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to SP|Q9FE63 Profilin 5 {Arabidopsis thaliana} Length = 168 Score = 83.8 bits (198), Expect = 4e-17 Identities = 47/119 (39%), Positives = 65/119 (54%), Gaps = 5/119 (4%) Frame = +1 Query: 55 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 222 MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I F L Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97 Query: 223 TSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 396 G+ + G +Y+ + G + +IR K G GV KT A+V +Y+EP+ P Q VV Sbjct: 98 APTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVV 156 >At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis thaliana]; identical to cDNA profilin (PRF2) GI:9965570 Length = 131 Score = 81.0 bits (191), Expect = 3e-16 Identities = 48/119 (40%), Positives = 64/119 (53%), Gaps = 5/119 (4%) Frame = +1 Query: 55 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 222 MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I FE L Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60 Query: 223 TSGGVTIAGTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 396 G+ + G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q VV Sbjct: 61 APTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQCNLVV 119 >At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen Ara t 8 identical to profilin 1 (Allergen Ara t 8) SP:Q42449 GI:1353770 from [Arabidopsis thaliana] Length = 131 Score = 80.2 bits (189), Expect = 5e-16 Identities = 47/119 (39%), Positives = 62/119 (52%), Gaps = 5/119 (4%) Frame = +1 Query: 55 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 222 MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+ I FE L Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60 Query: 223 TSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVV 396 G+ + G +Y+ + G +IR K G GV KT QA+V Y+EP+ Q VV Sbjct: 61 APTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQCNLVV 119 >At1g62420.1 68414.m07042 expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 465 Score = 31.5 bits (68), Expect = 0.21 Identities = 15/61 (24%), Positives = 31/61 (50%) Frame = +3 Query: 213 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 392 I +E RR +H+P W R + + W G ++ T++ ++ + T+ T+A + C Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255 Query: 393 R 395 + Sbjct: 256 K 256 >At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar to sodium/calcium exchanger protein [Mus musculus] gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 559 Score = 29.1 bits (62), Expect = 1.1 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = -1 Query: 287 MIWSVPLR*MYRVPAIVTPPLVSSDSFSKPATI 189 +IW++ L +P I+T P+VS D +SKP + Sbjct: 307 VIWAITLP--LNLPRILTIPVVSEDKWSKPLAV 337 >At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma membrane-type, putative / Ca2+-ATPase, putative (ACA7) identical to SP|O64806 Potential calcium-transporting ATPase 7, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong similarity to SP|O81108 Calcium-transporting ATPase 2, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2) {Arabidopsis thaliana} Length = 1015 Score = 28.3 bits (60), Expect = 2.0 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +1 Query: 148 KSEGFEISKDEVAKIVAGFENESLLTSGGV 237 K+ GF+I DE+ IV G + + L GGV Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119 >At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical to SP|O22218 Calcium-transporting ATPase 4, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4) {Arabidopsis thaliana} Length = 1030 Score = 27.9 bits (59), Expect = 2.6 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 148 KSEGFEISKDEVAKIVAGFENESLLTSGGV 237 K GF I DE+A +V + +SL GGV Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGV 118 >At4g37390.1 68417.m05294 auxin-responsive GH3 family protein similar to auxin-responsive GH3 product [Glycine max] GI:18591; contains Pfam profile PF03321: GH3 auxin-responsive promoter Length = 603 Score = 27.5 bits (58), Expect = 3.5 Identities = 19/66 (28%), Positives = 31/66 (46%) Frame = +1 Query: 31 YF*LNLIKMSWQDYVDKQLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVAGFEN 210 YF +NL M V +M + + HDG+ A++ E S E+A + G E Sbjct: 344 YFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEY 403 Query: 211 ESLLTS 228 E ++T+ Sbjct: 404 ELVITT 409 >At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma membrane-type / Ca(2+)-ATPase isoform 2 (ACA2) identical to SP|O81108 Calcium-transporting ATPase 2, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2) {Arabidopsis thaliana} Length = 1014 Score = 27.1 bits (57), Expect = 4.6 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 148 KSEGFEISKDEVAKIVAGFENESLLTSGGV 237 K+ GFEI DE+ IV + + L GGV Sbjct: 90 KAAGFEICADELGSIVESHDVKKLKFHGGV 119 >At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid envelope ATPase 1 (PEA1) identical to SP|Q37145 Calcium-transporting ATPase 1, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope ATPase 1) {Arabidopsis thaliana}; identical to cDNA envelope Ca2+-ATPase (PEA1) chloroplast gene encoding chloroplast protein GI:509809 Length = 946 Score = 26.6 bits (56), Expect = 6.1 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +1 Query: 148 KSEGFEISKDEVAKIVAGFENESLLTSGG 234 + GFEI DE+ IV G + + L GG Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGG 120 >At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid envelope ATPase 1 (PEA1) identical to SP|Q37145 Calcium-transporting ATPase 1, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope ATPase 1) {Arabidopsis thaliana}; identical to cDNA envelope Ca2+-ATPase (PEA1) chloroplast gene encoding chloroplast protein GI:509809 Length = 1020 Score = 26.6 bits (56), Expect = 6.1 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +1 Query: 148 KSEGFEISKDEVAKIVAGFENESLLTSGG 234 + GFEI DE+ IV G + + L GG Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGG 120 >At1g23550.1 68414.m02962 expressed protein Length = 323 Score = 26.6 bits (56), Expect = 6.1 Identities = 14/47 (29%), Positives = 24/47 (51%), Gaps = 1/47 (2%) Frame = +1 Query: 82 QLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVA-GFENESL 219 + +A R T A H G+ K + S+DE+ +I++ GF N + Sbjct: 92 KFLAFRIFTDAVARKHGGDANVKYGWYAGSRDEIQRIISYGFSNRDV 138 >At1g12940.1 68414.m01503 high-affinity nitrate transporter, putative similar to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI:3608362 Length = 502 Score = 26.6 bits (56), Expect = 6.1 Identities = 11/28 (39%), Positives = 18/28 (64%), Gaps = 1/28 (3%) Frame = +1 Query: 130 DGNVWAKSEGFEISKDEVAKIVA-GFEN 210 DG+ WA + E KD+V K+++ G +N Sbjct: 233 DGDYWAMHKSGEREKDDVGKVISNGIKN 260 >At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase, putative strong similarity to SP|P54151 Peptide methionine sulfoxide reductase (EC 1.8.4.6) {Brassica napus}; contains Pfam profile PF01625: Peptide methionine sulfoxide reductase Length = 258 Score = 26.2 bits (55), Expect = 8.0 Identities = 13/31 (41%), Positives = 19/31 (61%), Gaps = 2/31 (6%) Frame = -1 Query: 242 IVTPPLVSSDSFSKPATIF--ATSSFEISKP 156 +V+PPL+++ S SKP A SF +KP Sbjct: 5 VVSPPLIAAASLSKPLNSLSKAALSFSRAKP 35 >At4g04090.1 68417.m00578 speckle-type POZ protein-related contains Pfam PF00651 : BTB/POZ domain; similar to Speckle-type POZ protein (SP:O43791) [Homo sapiens] Length = 192 Score = 26.2 bits (55), Expect = 8.0 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +1 Query: 31 YF*LNLIKMSWQDYVDKQLMASRCVTKAAIAGHDGNVWAKSEGFE 165 +F +K+ + VD +LMA AAI+ H + A+SE FE Sbjct: 9 FFYDEFLKVLKEQQVDVRLMARDSEKDAAISAHKRILSARSEVFE 53 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,124,287 Number of Sequences: 28952 Number of extensions: 179495 Number of successful extensions: 433 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 414 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 423 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 575830496 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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