BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0210
(496 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g49310.1 68418.m06102 importin alpha-1 subunit, putative simi... 36 0.020
At5g58980.1 68418.m07389 ceramidase family protein contains Pfam... 34 0.060
At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2) sim... 33 0.14
At5g23150.1 68418.m02707 PWWP domain-containing protein identica... 32 0.18
At5g40450.1 68418.m04905 expressed protein 30 0.74
At1g07380.1 68414.m00787 ceramidase family protein contains simi... 30 0.74
At5g15560.1 68418.m01822 hypothetical protein 29 1.3
At2g38010.2 68415.m04666 ceramidase family protein contains Pfam... 29 2.3
At2g38010.1 68415.m04665 ceramidase family protein contains Pfam... 29 2.3
At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMP... 28 3.0
At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMP... 28 3.0
At2g47680.1 68415.m05955 zinc finger (CCCH type) helicase family... 28 3.0
At1g65470.1 68414.m07427 chromatin assembly factor-1 (FASCIATA1)... 28 4.0
At1g65180.1 68414.m07390 DC1 domain-containing protein contains ... 28 4.0
At3g31310.1 68416.m03983 hypothetical protein 27 5.3
At5g52000.1 68418.m06453 importin alpha-1 subunit, putative simi... 27 6.9
At1g26360.1 68414.m03216 hydrolase, alpha/beta fold family prote... 27 6.9
At3g08010.1 68416.m00978 expressed protein 27 9.2
At1g47900.1 68414.m05334 expressed protein 27 9.2
At1g03680.1 68414.m00347 thioredoxin M-type 1, chloroplast (TRX-... 27 9.2
At1g02690.2 68414.m00220 importin alpha-2 subunit, putative simi... 27 9.2
At1g02690.1 68414.m00219 importin alpha-2 subunit, putative simi... 27 9.2
>At5g49310.1 68418.m06102 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 519
Score = 35.5 bits (78), Expect = 0.020
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = -1
Query: 205 LVQHSQRAKQVVIVPAVRSFGNIKTAFTEETECV 104
LV+ Q A VV+VPA+R GNI + +++T CV
Sbjct: 287 LVELLQHASPVVLVPALRCIGNIVSGNSQQTHCV 320
>At5g58980.1 68418.m07389 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 733
Score = 33.9 bits (74), Expect = 0.060
Identities = 19/84 (22%), Positives = 42/84 (50%)
Frame = +2
Query: 173 HLFSSLGMLHECSPSMMSTADKSFYGTHEQCQSNEDIFNCEEEIDENLERAPTSMLPDED 352
+L +SLG +H+ +++ ++S HE + + N E +D + R+P++ L +
Sbjct: 134 YLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL---N 190
Query: 353 DPLSPENLVQDNVKHFCRLMKFAN 424
+P + + +V L+KF +
Sbjct: 191 NPAHERSKYEYDVDKEMTLVKFVD 214
>At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2)
similar to importin alpha 2 [Capsicum annuum]
GI:13752562; contains Pfam profiles PF01749: Importin
beta binding domain, PF00514:
Armadillo/beta-catenin-like repeat; non-consensus GG
donor splice site at exon 1 and 6; CT acceptor splice
site at exon 2
Length = 444
Score = 32.7 bits (71), Expect = 0.14
Identities = 16/36 (44%), Positives = 22/36 (61%)
Frame = -1
Query: 205 LVQHSQRAKQVVIVPAVRSFGNIKTAFTEETECVPS 98
LV+ Q V++PA+RS GNI T +T+CV S
Sbjct: 294 LVELLQHQSPSVLIPALRSIGNIVTGDDLQTQCVIS 329
>At5g23150.1 68418.m02707 PWWP domain-containing protein identical
to cDNA putative transcription factor (HUA2) GI:4868119;
contains Pfam profile PF00855: PWWP domain
Length = 1392
Score = 32.3 bits (70), Expect = 0.18
Identities = 18/59 (30%), Positives = 28/59 (47%)
Frame = +2
Query: 194 MLHECSPSMMSTADKSFYGTHEQCQSNEDIFNCEEEIDENLERAPTSMLPDEDDPLSPE 370
M H SPS + DKS G EQ + + N + ++L++ + DEDD P+
Sbjct: 390 MEHATSPSFSGSRDKSGKGHLEQKDRSSPVRNVKAPAAQSLKKRRAVCIYDEDDDEDPK 448
>At5g40450.1 68418.m04905 expressed protein
Length = 2910
Score = 30.3 bits (65), Expect = 0.74
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +2
Query: 275 EDIFNCEEEIDENLERAPTSMLPD-EDDPLSPE 370
EDIF+ E E+ AP SML ED+P++P+
Sbjct: 2103 EDIFSTETVPKESFIEAPVSMLASGEDEPVTPQ 2135
>At1g07380.1 68414.m00787 ceramidase family protein contains
similarity to mitochondrial ceramidase [Homo sapiens]
gi|9246993|gb|AAF86240
Length = 779
Score = 30.3 bits (65), Expect = 0.74
Identities = 18/84 (21%), Positives = 39/84 (46%)
Frame = +2
Query: 173 HLFSSLGMLHECSPSMMSTADKSFYGTHEQCQSNEDIFNCEEEIDENLERAPTSMLPDED 352
++ +SLG + + +++ + S HE + N E +D + R+P++ L +
Sbjct: 142 YIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYL---N 198
Query: 353 DPLSPENLVQDNVKHFCRLMKFAN 424
+P + + NV L+KF +
Sbjct: 199 NPSKERSKHKYNVDKEMTLLKFVD 222
>At5g15560.1 68418.m01822 hypothetical protein
Length = 239
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/66 (25%), Positives = 29/66 (43%)
Frame = +2
Query: 284 FNCEEEIDENLERAPTSMLPDEDDPLSPENLVQDNVKHFCRLMKFANGHSPALYFSMDPE 463
FN +E+ N+ EDD + ++QD++ CR N S + + E
Sbjct: 70 FNSKEQDISNVTSKQKDKATTEDDDDDVKEIIQDDISICCRSEASNNSISSFTFPILHNE 129
Query: 464 EERNVT 481
E+ +VT
Sbjct: 130 EDESVT 135
>At2g38010.2 68415.m04666 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 792
Score = 28.7 bits (61), Expect = 2.3
Identities = 17/84 (20%), Positives = 41/84 (48%)
Frame = +2
Query: 173 HLFSSLGMLHECSPSMMSTADKSFYGTHEQCQSNEDIFNCEEEIDENLERAPTSMLPDED 352
++ +SLG + + +++ ++S HE + N + +D + R+P+S L +
Sbjct: 131 YIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYL---N 187
Query: 353 DPLSPENLVQDNVKHFCRLMKFAN 424
+P + + + +V L+KF +
Sbjct: 188 NPAAERSKYKYDVDKEMTLVKFVD 211
>At2g38010.1 68415.m04665 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 757
Score = 28.7 bits (61), Expect = 2.3
Identities = 17/84 (20%), Positives = 41/84 (48%)
Frame = +2
Query: 173 HLFSSLGMLHECSPSMMSTADKSFYGTHEQCQSNEDIFNCEEEIDENLERAPTSMLPDED 352
++ +SLG + + +++ ++S HE + N + +D + R+P+S L +
Sbjct: 131 YIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYL---N 187
Query: 353 DPLSPENLVQDNVKHFCRLMKFAN 424
+P + + + +V L+KF +
Sbjct: 188 NPAAERSKYKYDVDKEMTLVKFVD 211
>At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMPA1)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 532
Score = 28.3 bits (60), Expect = 3.0
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1
Query: 172 VIVPAVRSFGNIKTAFTEETECV 104
V++PA+R+ GNI T +T+CV
Sbjct: 300 VLIPALRTVGNIVTGDDIQTQCV 322
>At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMPA1)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 532
Score = 28.3 bits (60), Expect = 3.0
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1
Query: 172 VIVPAVRSFGNIKTAFTEETECV 104
V++PA+R+ GNI T +T+CV
Sbjct: 300 VLIPALRTVGNIVTGDDIQTQCV 322
>At2g47680.1 68415.m05955 zinc finger (CCCH type) helicase family
protein similar to SP|Q28141 ATP-dependent RNA helicase
A (Nuclear DNA helicase II) (DEAD-box protein 9) {Bos
taurus}; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 1015
Score = 28.3 bits (60), Expect = 3.0
Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 9/77 (11%)
Frame = +2
Query: 185 SLGMLHECSPSMMSTADK--SFYGTHE-------QCQSNEDIFNCEEEIDENLERAPTSM 337
+L H P +S++D ++Y +E +CQ +ED + E++D N + P +
Sbjct: 616 TLSSFHRFRPQFISSSDSQPTYYNPYEFDHTCYIECQPSEDKYLHSEDVDNN--QPPPEV 673
Query: 338 LPDEDDPLSPENLVQDN 388
P P N Q N
Sbjct: 674 RKCVSVPFVPPNAFQAN 690
>At1g65470.1 68414.m07427 chromatin assembly factor-1 (FASCIATA1)
(FAS1) identical to FAS1 [Arabidopsis thaliana]
GI:4887626
Length = 815
Score = 27.9 bits (59), Expect = 4.0
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +2
Query: 257 EQCQSNEDIFNCEEEIDENLERAPTSMLPDEDD 355
E+ ++ E + +CE++ DE+LE S DEDD
Sbjct: 527 EEEEAGESLSDCEKDEDESLEEG-CSKADDEDD 558
>At1g65180.1 68414.m07390 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 653
Score = 27.9 bits (59), Expect = 4.0
Identities = 14/53 (26%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +2
Query: 254 HEQCQSNEDIFNCEEEIDENLERAPTSMLPDEDDPLSPENLVQDN-VKHFCRL 409
H +C + ED+++ E DE P+ED+P+ P ++ + ++HF +
Sbjct: 331 HTKCATREDVWDGIEMKDE----------PEEDEPMEPFEVISEGIIRHFSHI 373
>At3g31310.1 68416.m03983 hypothetical protein
Length = 291
Score = 27.5 bits (58), Expect = 5.3
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = +2
Query: 275 EDIFNCEEEIDENLERAPTSMLPDEDDPLSPENLVQDNVKHFCR 406
ED+ C++ NL A S+L D L L D HF R
Sbjct: 185 EDLLLCDKAASANLIGACASLLLPPPDTLLEFELYADTASHFLR 228
>At5g52000.1 68418.m06453 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 441
Score = 27.1 bits (57), Expect = 6.9
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -1
Query: 205 LVQHSQRAKQVVIVPAVRSFGNIKTAFTEETECV 104
LVQ Q VV+VPA+ + G + ++T+CV
Sbjct: 217 LVQILQLPSPVVLVPALLTIGAMTAGNHQQTQCV 250
>At1g26360.1 68414.m03216 hydrolase, alpha/beta fold family protein
similar to SP|Q40708 PIR7A protein {Oryza sativa},
ethylene-induced esterase [Citrus sinensis] GI:14279437,
polyneuridine aldehyde esterase [Rauvolfia serpentina]
GI:6651393; contains Pfam profile PF00561: alpha/beta
hydrolase fold
Length = 444
Score = 27.1 bits (57), Expect = 6.9
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -3
Query: 284 KYLRSTDTVRGFRRNFCLPSTSCWG 210
KY+R + FRR LPS SC G
Sbjct: 31 KYVRRLSLMPSFRRRTLLPSLSCSG 55
>At3g08010.1 68416.m00978 expressed protein
Length = 374
Score = 26.6 bits (56), Expect = 9.2
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = +2
Query: 170 HHLFSSLGMLHECSPSMMSTADKSFYGTHEQCQSNE 277
HHLFSS +L C S + S T EQ +NE
Sbjct: 32 HHLFSSETLLKRCRFVSRSLPESSLSITKEQEVANE 67
>At1g47900.1 68414.m05334 expressed protein
Length = 1054
Score = 26.6 bits (56), Expect = 9.2
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +2
Query: 257 EQCQSNEDIFNCEEEIDENLERAPTSMLPDEDD 355
E+C E I C E++E+++R + L EDD
Sbjct: 883 EKCNHQEAILRC-HELEEHIQRHRNTSLVAEDD 914
>At1g03680.1 68414.m00347 thioredoxin M-type 1, chloroplast (TRX-M1)
nearly identical to SP|O48737 Thioredoxin M-type 1,
chloroplast precursor (TRX-M1) {Arabidopsis thaliana};
similar to ESTs gb|T13714, gb|H76398, gb|N37762,
gb|AA042639, gb|T21104, emb|Z30901
Length = 179
Score = 26.6 bits (56), Expect = 9.2
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = +3
Query: 198 CTSAAPA*CRRQTKVSTEPTNSVSRTKIFLIV 293
CTS P R + ++++ PT S S ++F ++
Sbjct: 6 CTSRPPISIRSEMRIASSPTGSFSTRQMFSVL 37
>At1g02690.2 68414.m00220 importin alpha-2 subunit, putative similar
to importin alpha-2 subunit (Karyopherin alpha-2
subunit) (KAP alpha) SP:O04294 from [Arabidopsis
thaliana]
Length = 539
Score = 26.6 bits (56), Expect = 9.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -1
Query: 205 LVQHSQRAKQVVIVPAVRSFGNIKTAFTEETECVPS 98
LVQ V++PA+R+ GNI T +T+ V S
Sbjct: 294 LVQLLAHPSPSVLIPALRTIGNIVTGDDIQTQAVIS 329
>At1g02690.1 68414.m00219 importin alpha-2 subunit, putative similar
to importin alpha-2 subunit (Karyopherin alpha-2
subunit) (KAP alpha) SP:O04294 from [Arabidopsis
thaliana]
Length = 538
Score = 26.6 bits (56), Expect = 9.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -1
Query: 205 LVQHSQRAKQVVIVPAVRSFGNIKTAFTEETECVPS 98
LVQ V++PA+R+ GNI T +T+ V S
Sbjct: 293 LVQLLAHPSPSVLIPALRTIGNIVTGDDIQTQAVIS 328
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,192,208
Number of Sequences: 28952
Number of extensions: 201721
Number of successful extensions: 625
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 611
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 625
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 868578304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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