Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0148
(667 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 224 4e-59
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 223 8e-59
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 208 2e-54
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 44 9e-05
At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative... 42 5e-04
At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative... 40 0.001
At3g58750.1 68416.m06548 citrate synthase, glyoxysomal, putative... 38 0.006
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 30 1.2
At5g53030.2 68418.m06586 expressed protein 29 2.8
At5g53030.1 68418.m06587 expressed protein 29 2.8
At5g41770.1 68418.m05086 crooked neck protein, putative / cell c... 28 4.9
At4g16442.1 68417.m02489 integral membrane family protein contai... 28 4.9
At4g02260.3 68417.m00305 RelA/SpoT protein, putative (RSH1) iden... 28 4.9
At4g02260.2 68417.m00306 RelA/SpoT protein, putative (RSH1) iden... 28 4.9
At4g02260.1 68417.m00307 RelA/SpoT protein, putative (RSH1) iden... 28 4.9
At3g15750.1 68416.m01995 expressed protein 28 4.9
At3g52890.2 68416.m05829 protein kinase (KIPK) identical to prot... 28 6.4
At3g52890.1 68416.m05828 protein kinase (KIPK) identical to prot... 28 6.4
At5g54880.1 68418.m06836 DTW domain-containing protein contains ... 27 8.5
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 224 bits (548), Expect = 4e-59
Identities = 100/206 (48%), Positives = 147/206 (71%)
Frame = +1
Query: 49 MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGST 228
++ F SR+ Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 7 VSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKV 66
Query: 229 KVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEG 408
++G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPEG
Sbjct: 67 QLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEG 126
Query: 409 LFWLLVTGDIPTEAQAKALSKEWAARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSE 588
L WLL+TG +P++ Q +ALSK+ A RA +P +V ++ +P HPM+QF++ V AL +
Sbjct: 127 LLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQ 186
Query: 589 SKFAKAYSEGVHKSKYWEYVYEDTMN 666
S+F KAY G+HKSK+WE YED +N
Sbjct: 187 SEFQKAYENGIHKSKFWEPTYEDCLN 212
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 223 bits (545), Expect = 8e-59
Identities = 96/178 (53%), Positives = 136/178 (76%)
Frame = +1
Query: 133 SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLD 312
S+ +LKS LQE IP++Q+++++ + +HG ++G +TVDM+ GGMRG+ GL+WETS+LD
Sbjct: 34 SSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSLLD 93
Query: 313 ADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAE 492
+EGIRFRGLSIPECQ+ LP A+ G EPLPEGL WLL+TG +P++ Q +ALSK+ A RA
Sbjct: 94 PEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANRAA 153
Query: 493 LPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAYSEGVHKSKYWEYVYEDTMN 666
+P +V ++ +P HPM+QF++ V AL +S+F KAY G+HKSK+WE YED +N
Sbjct: 154 VPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQKAYENGIHKSKFWEPTYEDCLN 211
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 208 bits (509), Expect = 2e-54
Identities = 96/174 (55%), Positives = 131/174 (75%), Gaps = 1/174 (0%)
Frame = +1
Query: 148 NLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGI 327
+LKS +QE IP++Q+++++ + + G VG +TVDM+ GGMRG+ GL+WETS+LDADEGI
Sbjct: 5 DLKSQMQEIIPEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGI 64
Query: 328 RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAELPAHV 507
RFRG+SIPECQ+ LP A+ GEEPLPE L WLL+TG +PT+ QA ALS E A RA +PA
Sbjct: 65 RFRGMSIPECQKILPSAESGEEPLPESLLWLLLTGKVPTKEQANALSTELAHRAAVPA-- 122
Query: 508 VTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAYSEG-VHKSKYWEYVYEDTMN 666
++ +P HPM+QF++ V AL +S+F KAY +G + KSKYWE +ED +N
Sbjct: 123 ---IDALPSTAHPMTQFASGVMALQVQSEFQKAYEQGDISKSKYWEPTFEDALN 173
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 44.0 bits (99), Expect = 9e-05
Identities = 17/34 (50%), Positives = 25/34 (73%)
Frame = +1
Query: 187 QEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGL 288
Q++ ++ + KHG VG +TVDM+ GGMRG+ GL
Sbjct: 43 QDRSKKLKLKHGKVPVGNITVDMVLGGMRGMTGL 76
>At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 480
Score = 41.5 bits (93), Expect = 5e-04
Identities = 31/114 (27%), Positives = 56/114 (49%), Gaps = 3/114 (2%)
Frame = +1
Query: 301 SVLDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEW 477
S +D DEGI R+RG + E ++ + + +LL+ G++P++ Q
Sbjct: 107 SYIDGDEGILRYRGYPVEELAEKSTYTE---------VTYLLIYGNLPSQRQLADWEFAI 157
Query: 478 AARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFA--KAYSEGVHKSK 633
+ + +P V+ M+ +MP +HP+ A++AL+ A GV+KSK
Sbjct: 158 SQNSAVPQGVLDMIQSMPNDVHPVGALVTAMSALSIFYPDANPSLMGLGVYKSK 211
>At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 509
Score = 40.3 bits (90), Expect = 0.001
Identities = 27/95 (28%), Positives = 49/95 (51%), Gaps = 1/95 (1%)
Frame = +1
Query: 301 SVLDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEW 477
S +D DEGI R+RG I E + + + +LL+ G++P+E+Q
Sbjct: 105 SYIDGDEGILRYRGYPIEEMAENSTFLE---------VAYLLMYGNLPSESQLSDWEFAV 155
Query: 478 AARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALN 582
+ + +P V+ ++ +MP HPM +A++AL+
Sbjct: 156 SQHSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALS 190
>At3g58750.1 68416.m06548 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 514
Score = 37.9 bits (84), Expect = 0.006
Identities = 30/112 (26%), Positives = 55/112 (49%), Gaps = 3/112 (2%)
Frame = +1
Query: 307 LDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAA 483
+D DEGI R+RG I E + + + +LL+ G++P+++Q +
Sbjct: 112 IDGDEGILRYRGYPIEELAESSTFIE---------VAYLLMYGNLPSQSQLADWEFTVSQ 162
Query: 484 RAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAYSEG--VHKSK 633
+ +P V+ ++ +MP HPM +A++AL+ A G ++KSK
Sbjct: 163 HSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALSGQDIYKSK 214
>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
gi|6520232|dbj|AB028233.1|
Length = 492
Score = 30.3 bits (65), Expect = 1.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +2
Query: 524 ICPANCIPCRSSRLPXPHSTVNLNSLKPTQR 616
I P+NC+PC +S +P +L+S P+ R
Sbjct: 157 ILPSNCLPCLNSTVPSIEKRRSLSSSPPSTR 187
>At5g53030.2 68418.m06586 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 2.8
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 298 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 405
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g53030.1 68418.m06587 expressed protein
Length = 245
Score = 29.1 bits (62), Expect = 2.8
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 298 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 405
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g41770.1 68418.m05086 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 705
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +1
Query: 157 SILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMY 261
SI+Q K+PK+ +K + ++ GST+ E +D +Y
Sbjct: 643 SIVQSKLPKKLKKRKAITREDGSTEYEEY-IDYLY 676
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 28.3 bits (60), Expect = 4.9
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +2
Query: 245 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSA 358
Q + C V ++ KV F KPL W ++ Y +++A
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAA 108
>At4g02260.3 68417.m00305 RelA/SpoT protein, putative (RSH1)
identical to RSH1 (RelA/SpoT homolog) [Arabidopsis
thaliana] GI:7141304; contains Pfam profiles PF02824:
TGS domain, PF01966: HD domain, PF04607: Region found in
RelA / SpoT proteins
Length = 816
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 353 SANSNCPRLRVEKNHYPKVFSGFSSLAIYPPKRKLKRCLKNGRRGR 490
+A S+C L P+ +GF + +PP + C +NGR+ R
Sbjct: 25 NARSDCSALSCAWKA-PRALTGFLASTAHPPVCSVYSCGRNGRKSR 69
>At4g02260.2 68417.m00306 RelA/SpoT protein, putative (RSH1)
identical to RSH1 (RelA/SpoT homolog) [Arabidopsis
thaliana] GI:7141304; contains Pfam profiles PF02824:
TGS domain, PF01966: HD domain, PF04607: Region found in
RelA / SpoT proteins
Length = 883
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 353 SANSNCPRLRVEKNHYPKVFSGFSSLAIYPPKRKLKRCLKNGRRGR 490
+A S+C L P+ +GF + +PP + C +NGR+ R
Sbjct: 25 NARSDCSALSCAWKA-PRALTGFLASTAHPPVCSVYSCGRNGRKSR 69
>At4g02260.1 68417.m00307 RelA/SpoT protein, putative (RSH1)
identical to RSH1 (RelA/SpoT homolog) [Arabidopsis
thaliana] GI:7141304; contains Pfam profiles PF02824:
TGS domain, PF01966: HD domain, PF04607: Region found in
RelA / SpoT proteins
Length = 884
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 353 SANSNCPRLRVEKNHYPKVFSGFSSLAIYPPKRKLKRCLKNGRRGR 490
+A S+C L P+ +GF + +PP + C +NGR+ R
Sbjct: 25 NARSDCSALSCAWKA-PRALTGFLASTAHPPVCSVYSCGRNGRKSR 69
>At3g15750.1 68416.m01995 expressed protein
Length = 186
Score = 28.3 bits (60), Expect = 4.9
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +1
Query: 118 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDM 255
L+RG+S+ L L+EK+ +QE +F+K H S V M
Sbjct: 79 LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSSVHALSTEVAM 124
>At3g52890.2 68416.m05829 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 6.4
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +1
Query: 334 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 447
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
>At3g52890.1 68416.m05828 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 6.4
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +1
Query: 334 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 447
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
>At5g54880.1 68418.m06836 DTW domain-containing protein contains
Pfam domain, PF03942: DTW domain
Length = 394
Score = 27.5 bits (58), Expect = 8.5
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 533 ANCIPCRSSRLPXPHSTVNLNSLKPTQRACTNPSIGSTCTKTR 661
A+CI C+ R+ T+ + + +PT +C PS C K R
Sbjct: 25 ADCIRCQGVRVS---KTLKMGTKRPTCPSCDKPSQLCLCKKMR 64
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.130 0.380
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,287,719
Number of Sequences: 28952
Number of extensions: 289178
Number of successful extensions: 1092
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1059
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1090
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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