BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Nnor0117 (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 132 2e-31 At3g07770.1 68416.m00947 heat shock protein-related strong simil... 130 4e-31 At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 130 7e-31 At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 129 1e-30 At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 128 2e-30 At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 128 2e-30 At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 124 5e-29 At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 115 2e-26 At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 115 2e-26 At4g32350.1 68417.m04605 expressed protein contains Pfam profile... 31 0.36 At1g67230.1 68414.m07652 expressed protein 28 4.4 At4g02460.1 68417.m00333 DNA mismatch repair protein, putative s... 27 5.8 At5g65910.1 68418.m08296 BSD domain-containing protein contains ... 27 7.7 >At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly identical to SP|P55737 Heat shock protein 81-2 (HSP81-2) {Arabidopsis thaliana} Length = 699 Score = 132 bits (318), Expect = 2e-31 Identities = 70/174 (40%), Positives = 93/174 (53%), Gaps = 6/174 (3%) Frame = +1 Query: 10 EKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGD 186 E AA MIGQFGVGFYSA+LVAD V V +KHNDD Q+VW+S A SF+V D G+ Sbjct: 107 EALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGE 166 Query: 187 TLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW-----ASXXXXXXXXXX 351 TL RGT + L++KE+ +YL+ ++ LVKK+S+FI++PI LW Sbjct: 167 TLGRGTKMVLYLKEDQLEYLEERRLKDLVKKHSEFISYPISLWIEKTIEKEISDDEEEEE 226 Query: 352 XXXXXXXXXXXXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEY 513 +W+L+N KPIW RKP E+ +EY Sbjct: 227 KKDEEGKVEEVDEEKEKEEKKKKKIKEVSHEWDLVNKQKPIWMRKPEEINKEEY 280 >At3g07770.1 68416.m00947 heat shock protein-related strong similarity to heat-shock protein [Secale cereale] GI:556673; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 803 Score = 130 bits (315), Expect = 4e-31 Identities = 68/166 (40%), Positives = 91/166 (54%), Gaps = 4/166 (2%) Frame = +1 Query: 34 NDMIGQFGVGFYSAFLVADSVTVASKH-NDDRQHVWQSDANS--FSVAEDPRGDTL-KRG 201 N++IGQFGVGFYSAFLVAD V V++K D+Q+VW+ +ANS F++ ED +L RG Sbjct: 209 NNLIGQFGVGFYSAFLVADRVIVSTKSPKSDKQYVWEGEANSSSFTIQEDTDPQSLIPRG 268 Query: 202 THLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLWASXXXXXXXXXXXXXXXXXXXX 381 T +TLH+K+EA ++ + I+ LVK YSQF++FPIY W Sbjct: 269 TRITLHLKQEAKNFADPERIQKLVKNYSQFVSFPIYTW-QEKGYTKEVEVEDDPTETKKD 327 Query: 382 XXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEYTQ 519 WDWEL N+ +PIW R P EV EY + Sbjct: 328 DQDDQTEKKKKTKKVVERYWDWELTNETQPIWLRNPKEVTTAEYNE 373 >At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly identical to heat shock protein hsp81.4 [Arabidopsis thaliana] GI:1906828; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 699 Score = 130 bits (313), Expect = 7e-31 Identities = 68/174 (39%), Positives = 92/174 (52%), Gaps = 6/174 (3%) Frame = +1 Query: 10 EKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGD 186 E AA MIGQFGVGFYSA+LVAD V V +KHNDD Q+VW+S A SF+V D G+ Sbjct: 107 EALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGE 166 Query: 187 TLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW-----ASXXXXXXXXXX 351 L RGT + L++KE+ +Y++ ++ LVKK+S+FI++PI LW Sbjct: 167 ALGRGTKMILYLKEDQMEYIEERRLKDLVKKHSEFISYPISLWIEKTIEKEISDDEEEEE 226 Query: 352 XXXXXXXXXXXXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEY 513 +W+L+N KPIW RKP E+ +EY Sbjct: 227 KKDEEGKVEEIDEEKEKEEKKKKKIKEVTHEWDLVNKQKPIWMRKPEEINKEEY 280 >At5g56010.1 68418.m06989 heat shock protein, putative strong similarity to SP|P55737 Heat shock protein 81-2 (HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 699 Score = 129 bits (312), Expect = 1e-30 Identities = 68/174 (39%), Positives = 92/174 (52%), Gaps = 6/174 (3%) Frame = +1 Query: 10 EKSAAPEMNDMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGD 186 E AA MIGQFGVGFYSA+LVAD V V +KHNDD Q+VW+S A SF+V D G+ Sbjct: 107 EALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGE 166 Query: 187 TLKRGTHLTLHMKEEASDYLQADTIRALVKKYSQFINFPIYLW-----ASXXXXXXXXXX 351 L RGT + L++KE+ +Y++ ++ LVKK+S+FI++PI LW Sbjct: 167 ALGRGTKMVLYLKEDQMEYIEERRLKDLVKKHSEFISYPISLWIEKTIEKEISDDEEEEE 226 Query: 352 XXXXXXXXXXXXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEY 513 +W+L+N KPIW RKP E+ +EY Sbjct: 227 KKDEEGKVEEVDEEKEKEEKKKKKIKEVSHEWDLVNKQKPIWMRKPEEINKEEY 280 >At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI:19570872; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 823 Score = 128 bits (309), Expect = 2e-30 Identities = 57/96 (59%), Positives = 76/96 (79%), Gaps = 1/96 (1%) Frame = +1 Query: 37 DMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDANS-FSVAEDPRGDTLKRGTHLT 213 ++IGQFGVGFYSA+LVAD + V SKHNDD Q+VW+S AN F+V+ED + L RGT + Sbjct: 190 NLIGQFGVGFYSAYLVADYIEVISKHNDDSQYVWESKANGKFAVSEDTWNEPLGRGTEIR 249 Query: 214 LHMKEEASDYLQADTIRALVKKYSQFINFPIYLWAS 321 LH+++EA +YL+ ++ LVK+YS+FINFPI LWAS Sbjct: 250 LHLRDEAGEYLEESKLKELVKRYSEFINFPISLWAS 285 Score = 44.0 bits (99), Expect = 6e-05 Identities = 16/28 (57%), Positives = 22/28 (78%) Frame = +1 Query: 436 VWDWELMNDNKPIWTRKPSEVQDDEYTQ 519 V++WEL+ND K IW R P EV ++EYT+ Sbjct: 335 VYEWELLNDVKAIWLRSPKEVTEEEYTK 362 >At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI:19570872; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 823 Score = 128 bits (309), Expect = 2e-30 Identities = 57/96 (59%), Positives = 76/96 (79%), Gaps = 1/96 (1%) Frame = +1 Query: 37 DMIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDANS-FSVAEDPRGDTLKRGTHLT 213 ++IGQFGVGFYSA+LVAD + V SKHNDD Q+VW+S AN F+V+ED + L RGT + Sbjct: 190 NLIGQFGVGFYSAYLVADYIEVISKHNDDSQYVWESKANGKFAVSEDTWNEPLGRGTEIR 249 Query: 214 LHMKEEASDYLQADTIRALVKKYSQFINFPIYLWAS 321 LH+++EA +YL+ ++ LVK+YS+FINFPI LWAS Sbjct: 250 LHLRDEAGEYLEESKLKELVKRYSEFINFPISLWAS 285 Score = 44.0 bits (99), Expect = 6e-05 Identities = 16/28 (57%), Positives = 22/28 (78%) Frame = +1 Query: 436 VWDWELMNDNKPIWTRKPSEVQDDEYTQ 519 V++WEL+ND K IW R P EV ++EYT+ Sbjct: 335 VYEWELLNDVKAIWLRSPKEVTEEEYTK 362 >At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat shock protein 83 (HSP83) nearly identical to SP|P27323 Heat shock protein 81-1 (HSP81-1) (Heat shock protein 83) {Arabidopsis thaliana}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 705 Score = 124 bits (298), Expect = 5e-29 Identities = 67/167 (40%), Positives = 90/167 (53%), Gaps = 9/167 (5%) Frame = +1 Query: 40 MIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDA-NSFSVAEDPRGDTLKRGTHLTL 216 MIGQFGVGFYSA+LVA+ V V +KHNDD Q+VW+S A SF+V D G+ L RGT +TL Sbjct: 122 MIGQFGVGFYSAYLVAEKVVVTTKHNDDEQYVWESQAGGSFTVTRDVDGEPLGRGTKITL 181 Query: 217 HMKEEASDYLQADTIRALVKKYSQFINFPIYLWA--------SXXXXXXXXXXXXXXXXX 372 +K++ +YL+ ++ LVKK+S+FI++PIYLW S Sbjct: 182 FLKDDQLEYLEERRLKDLVKKHSEFISYPIYLWTEKTTEKEISDDEDEDEPKKENEGEVE 241 Query: 373 XXXXXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEY 513 W+ L+N KPIW RKP E+ +EY Sbjct: 242 EVDEEKEKDGKKKKKIKEVSHEWE--LINKQKPIWLRKPEEITKEEY 286 >At2g04030.2 68415.m00372 heat shock protein, putative strong similarity to heat shock protein [Arabidopsis thaliana] GI:1906830; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 777 Score = 115 bits (276), Expect = 2e-26 Identities = 62/167 (37%), Positives = 90/167 (53%), Gaps = 5/167 (2%) Frame = +1 Query: 34 NDMIGQFGVGFYSAFLVADSVTVASKH-NDDRQHVWQS--DANSFSVAEDPRGDT-LKRG 201 N +IGQFGVGFYSAFLVA+ V V++K D+Q+VW+S D++S+ + E+ D L+RG Sbjct: 191 NGLIGQFGVGFYSAFLVAEKVVVSTKSPKSDKQYVWESVADSSSYLIREETDPDNILRRG 250 Query: 202 THLTLHMKEEAS-DYLQADTIRALVKKYSQFINFPIYLWASXXXXXXXXXXXXXXXXXXX 378 T +TL+++E+ ++ ++ I+ LVK YSQF+ FPIY W Sbjct: 251 TQITLYLREDDKYEFAESTRIKNLVKNYSQFVGFPIYTWQE-------KSRTIEVEEDEP 303 Query: 379 XXXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEYTQ 519 WDWEL N+ KP+W R EV+ EY + Sbjct: 304 VKEGEEGEPKKKKTTKTEKYWDWELANETKPLWMRNSKEVEKGEYNE 350 >At2g04030.1 68415.m00371 heat shock protein, putative strong similarity to heat shock protein [Arabidopsis thaliana] GI:1906830; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 780 Score = 115 bits (276), Expect = 2e-26 Identities = 62/167 (37%), Positives = 90/167 (53%), Gaps = 5/167 (2%) Frame = +1 Query: 34 NDMIGQFGVGFYSAFLVADSVTVASKH-NDDRQHVWQS--DANSFSVAEDPRGDT-LKRG 201 N +IGQFGVGFYSAFLVA+ V V++K D+Q+VW+S D++S+ + E+ D L+RG Sbjct: 191 NGLIGQFGVGFYSAFLVAEKVVVSTKSPKSDKQYVWESVADSSSYLIREETDPDNILRRG 250 Query: 202 THLTLHMKEEAS-DYLQADTIRALVKKYSQFINFPIYLWASXXXXXXXXXXXXXXXXXXX 378 T +TL+++E+ ++ ++ I+ LVK YSQF+ FPIY W Sbjct: 251 TQITLYLREDDKYEFAESTRIKNLVKNYSQFVGFPIYTWQE-------KSRTIEVEEDEP 303 Query: 379 XXXXXXXXXXXXXXXXXXXVWDWELMNDNKPIWTRKPSEVQDDEYTQ 519 WDWEL N+ KP+W R EV+ EY + Sbjct: 304 VKEGEEGEPKKKKTTKTEKYWDWELANETKPLWMRNSKEVEKGEYNE 350 >At4g32350.1 68417.m04605 expressed protein contains Pfam profile: PF03398 eukaryotic protein of unknown function, DUF292 Length = 732 Score = 31.5 bits (68), Expect = 0.36 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +1 Query: 85 ADSVTVASKHNDDRQHVWQSDANSFSVAEDPRGDTLKRGTH 207 A+S+ S+H++DR+ + S EDP GD +K G + Sbjct: 453 AESINPGSRHHNDRESKENAVLVGKSTEEDPSGDNVKGGEY 493 >At1g67230.1 68414.m07652 expressed protein Length = 1132 Score = 27.9 bits (59), Expect = 4.4 Identities = 12/23 (52%), Positives = 18/23 (78%) Frame = -3 Query: 312 QVNGEVDELRVLLDERADGVRLQ 244 ++N E DELRV +ER++ +RLQ Sbjct: 468 EINKEKDELRVTEEERSEYLRLQ 490 >At4g02460.1 68417.m00333 DNA mismatch repair protein, putative similar to SP|P54278 PMS1 protein homolog 2 (DNA mismatch repair protein PMS2) {Homo sapiens}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF01119: DNA mismatch repair protein, C-terminal domain Length = 923 Score = 27.5 bits (58), Expect = 5.8 Identities = 21/75 (28%), Positives = 37/75 (49%) Frame = +1 Query: 40 MIGQFGVGFYSAFLVADSVTVASKHNDDRQHVWQSDANSFSVAEDPRGDTLKRGTHLTLH 219 ++GQF +GF A L D + + +H D + ++ A S + + P L + +L L Sbjct: 707 VLGQFNLGFIIAKLERD-LFIVDQHAADEKFNFEHLARSTVLNQQP----LLQPLNLELS 761 Query: 220 MKEEASDYLQADTIR 264 +EE + + D IR Sbjct: 762 PEEEVTVLMHMDIIR 776 >At5g65910.1 68418.m08296 BSD domain-containing protein contains Pfam profile PF03909: BSD domain Length = 432 Score = 27.1 bits (57), Expect = 7.7 Identities = 11/34 (32%), Positives = 21/34 (61%) Frame = +1 Query: 82 VADSVTVASKHNDDRQHVWQSDANSFSVAEDPRG 183 +A+S+ + + DD +H+ Q + S S ++ PRG Sbjct: 8 IANSLKLDEEDEDDDKHLNQQPSESVSDSQSPRG 41 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.315 0.130 0.400 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,740,191 Number of Sequences: 28952 Number of extensions: 81584 Number of successful extensions: 327 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 303 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 315 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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