BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Nnor0110 (631 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 95 6e-21 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 55 1e-08 SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 48 8e-07 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 46 3e-06 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 45 1e-05 SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 35 0.011 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.42 SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharo... 27 2.2 SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|c... 27 3.0 SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 6.8 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 6.8 >SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1284 Score = 95.5 bits (227), Expect = 6e-21 Identities = 69/211 (32%), Positives = 105/211 (49%), Gaps = 2/211 (0%) Frame = +1 Query: 4 RAFELLRSGLDRSKYLLVKEAKIIAMTCTHAALKRSELVQMGFKYDNILMEESAQILEIE 183 R F LLR D+ Y L ++++II T T + + L + GF ++N+++ S I E Sbjct: 929 RPFGLLRYYEDQELYALCQQSRIIGCTWTSLSTRLGTLKEKGFCFNNLIVMNSQNISE-S 987 Query: 184 TFIPLLLQNPQDGRSRLKRWIMIGDHHQLPPVVKNMAFQKYCNMEQSLFTRMVRLGVPYV 363 + +LL N + + R +++G+ + N N SLF R+ L + Sbjct: 988 SITSILLSNCEP--TGFDRLVLLGNQYLTSG---NQDINNTSN--GSLFKRLRYLKSRII 1040 Query: 364 ELDAQGRARPSICNLYRWRYRALGDLCHVTQLPEYRA--ANAGLRHDFQLVNVDDFNGFG 537 +L+ Q R SI +L Y D+ V P R N+G H+ Q +NV F G Sbjct: 1041 DLNTQYNVRESISSLCSSIYPL--DIKTVDSSPNKRLDYGNSGFAHEVQFINVGAFKGSQ 1098 Query: 538 ETEPSPYFYQNLAEAEYVVAVFMYMRLIGWP 630 ETEP + QNL EAEY VA+F YMR++G+P Sbjct: 1099 ETEPVSGYKQNLGEAEYAVALFQYMRMLGYP 1129 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 54.8 bits (126), Expect = 1e-08 Identities = 38/116 (32%), Positives = 59/116 (50%) Frame = +1 Query: 52 LVKEAKIIAMTCTHAALKRSELVQMGFKYDNILMEESAQILEIETFIPLLLQNPQDGRSR 231 L++ A +I TC A +R +K+ ++L++E+ Q E E IPL+L Sbjct: 534 LLRAAHVICCTCVGAGDRRISK----YKFRSVLIDEATQASEPECMIPLVLG-------- 581 Query: 232 LKRWIMIGDHHQLPPVVKNMAFQKYCNMEQSLFTRMVRLGVPYVELDAQGRARPSI 399 K+ +++GDH QL PVV N ++ QSLF R++ LG L Q R P + Sbjct: 582 AKQVVLVGDHQQLGPVVMNKKV-ALASLSQSLFERLIILGNSPFRLVVQYRMHPCL 636 >SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochromatin assembly Hrr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1015 Score = 48.4 bits (110), Expect = 8e-07 Identities = 52/189 (27%), Positives = 78/189 (41%), Gaps = 1/189 (0%) Frame = +1 Query: 49 LLVKEAKIIAMTCTHAALKRSELVQMGFKYDNILMEESAQILEIETFIPLLLQNPQDGRS 228 +L++ A +I MT T R L ++ K +EE+A +LE IP + + Sbjct: 678 ILLRGANVIGMTTTGLNKYRDILERINPKI--CFIEEAADVLE-GPIIPAVFPS------ 728 Query: 229 RLKRWIMIGDHHQLPPVVKNMAF-QKYCNMEQSLFTRMVRLGVPYVELDAQGRARPSICN 405 L++ ++IGDH QL P A Q N+ S+F R+V + Y L Q R P I Sbjct: 729 -LEQLVLIGDHKQLRPGCSTYALRQDPFNLSISMFERLVENDMEYTRLTMQRRMHPQIRR 787 Query: 406 LYRWRYRALGDLCHVTQLPEYRAANAGLRHDFQLVNVDDFNGFGETEPSPYFYQNLAEAE 585 L Y L D P R ++D +GF NL EA+ Sbjct: 788 LVSSVYEDLSDYEITKYWPSIPGMGEIRRFFLTHSRIEDNDGFASK-------INLFEAQ 840 Query: 586 YVVAVFMYM 612 +V +Y+ Sbjct: 841 MLVQFAVYL 849 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 46.4 bits (105), Expect = 3e-06 Identities = 51/196 (26%), Positives = 88/196 (44%), Gaps = 6/196 (3%) Frame = +1 Query: 52 LVKEAKIIAMTCTHAALKRSELVQMGFKYDNILMEESAQILEIETFIPLLLQNPQDGRSR 231 L++EA I+ T + A L+ G + ++++E+AQ +E+ + IPL + Sbjct: 1484 LLQEADIVCATLS--ASGHELLLNAGLTFRTVIIDEAAQAVELSSIIPL--------KYG 1533 Query: 232 LKRWIMIGDHHQLPPVVKNMAFQKYCNMEQSLFTRMVRL-GVPYVELDAQGRARPSICN- 405 + +M+GD +QLPP V + K+ QSL+ RM + L Q R P I Sbjct: 1534 CESCVMVGDPNQLPPTVLSKTSAKF-GYSQSLYVRMFKQHNESACLLSIQYRMNPEISRF 1592 Query: 406 ----LYRWRYRALGDLCHVTQLPEYRAANAGLRHDFQLVNVDDFNGFGETEPSPYFYQNL 573 Y + ++ VT P + G+ ++ NV F ++ S Y N+ Sbjct: 1593 PSKFFYNSKLLDGPNMSAVTSRPWHEDPQLGI---YRFFNVHGTEAFSNSK-SLY---NV 1645 Query: 574 AEAEYVVAVFMYMRLI 621 EA ++ + +Y RLI Sbjct: 1646 EEASFI--LLLYERLI 1659 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 44.8 bits (101), Expect = 1e-05 Identities = 27/101 (26%), Positives = 53/101 (52%) Frame = +1 Query: 37 RSKYLLVKEAKIIAMTCTHAALKRSELVQMGFKYDNILMEESAQILEIETFIPLLLQNPQ 216 +++ ++K+A ++ T + + + + ++++E+AQ +E++T IPL Sbjct: 1341 KAQKAILKQADVVCATLSGSG--HDLVAHSSLNFSTVIIDEAAQAVELDTIIPL------ 1392 Query: 217 DGRSRLKRWIMIGDHHQLPPVVKNMAFQKYCNMEQSLFTRM 339 R K+ I++GD +QLPP V + N QSLF R+ Sbjct: 1393 --RYGAKKCILVGDPNQLPPTVLSKKAAS-LNYSQSLFVRI 1430 >SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1398 Score = 34.7 bits (76), Expect = 0.011 Identities = 19/57 (33%), Positives = 35/57 (61%) Frame = +1 Query: 133 KYDNILMEESAQILEIETFIPLLLQNPQDGRSRLKRWIMIGDHHQLPPVVKNMAFQK 303 K+D +++E++QI +P+ L Q ++++++GDH+QLPP+VKN K Sbjct: 1059 KFDYCIIDEASQIP-----LPICLGPLQ----LAEKFVLVGDHYQLPPLVKNSRTSK 1106 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 29.5 bits (63), Expect = 0.42 Identities = 20/61 (32%), Positives = 27/61 (44%), Gaps = 7/61 (11%) Frame = +3 Query: 138 RQHTDGGISSNPRDRDVHTAA---AAESAGRQVP----AEALDHDRRPPPAAPGRQEHGV 296 R + G I P+ R +A S GRQ P + A+ + PPPA PGR + Sbjct: 344 RSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPAL 403 Query: 297 P 299 P Sbjct: 404 P 404 Score = 25.0 bits (52), Expect = 9.0 Identities = 14/50 (28%), Positives = 19/50 (38%), Gaps = 1/50 (2%) Frame = +3 Query: 159 ISSNPRDRDVHTAAAAESAGR-QVPAEALDHDRRPPPAAPGRQEHGVPEV 305 +S NP + R A A + R PPP P R+ G P + Sbjct: 278 VSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPI 327 >SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 805 Score = 27.1 bits (57), Expect = 2.2 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +1 Query: 244 IMIGDHHQLPPVVKNMAFQKYCNMEQS 324 ++ GD QLPPV +N K+C Q+ Sbjct: 450 VLTGDFFQLPPVPENGKESKFCFESQT 476 >SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 819 Score = 26.6 bits (56), Expect = 3.0 Identities = 14/31 (45%), Positives = 17/31 (54%) Frame = +3 Query: 327 VHQNGQTGCSVRGTGRTGSS*TQHLQPVPLA 419 V Q G G + G+ T SS +QPVPLA Sbjct: 654 VQQRGNVGVTTTGSILTQSSPALPVQPVPLA 684 >SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 684 Score = 25.4 bits (53), Expect = 6.8 Identities = 9/33 (27%), Positives = 19/33 (57%) Frame = +1 Query: 163 AQILEIETFIPLLLQNPQDGRSRLKRWIMIGDH 261 +Q+ +E+FI L + +GR + + ++ DH Sbjct: 106 SQLARLESFIMTLKNSDPEGRDEMLKSVVFSDH 138 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 25.4 bits (53), Expect = 6.8 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Frame = +2 Query: 365 NWTHRVELDPA-SATCTAGATGRWATCAT*RSSRSTAPPTRACD 493 +W+ E+DP+ +A+ T +T AT + SS S+ A D Sbjct: 955 SWSSSSEVDPSTAASATGSSTSSIATASVSGSSTSSVATASATD 998 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,499,807 Number of Sequences: 5004 Number of extensions: 50712 Number of successful extensions: 144 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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