Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Nnor0060
         (395 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz...    64   6e-12
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo...    62   4e-11
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr...    41   6e-05
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces...    38   7e-04
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy...    35   0.004
SPBC146.03c |cut3|smc4, smc4|condensin subunit Cut3|Schizosaccha...    27   0.80 
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi...    27   1.4  
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac...    26   1.8  
SPBC29A3.02c |his7||phosphoribosyl-AMP cyclohydrolase/phosphorib...    25   4.3  
SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomy...    25   4.3  
SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr ...    25   5.6  
SPCC1919.02 |||pig-X|Schizosaccharomyces pombe|chr 3|||Manual          24   7.4  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    24   9.8  
SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyc...    24   9.8  

>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 676

 Score = 64.5 bits (150), Expect = 6e-12
 Identities = 41/131 (31%), Positives = 64/131 (48%), Gaps = 4/131 (3%)
 Frame = +1

Query: 13  WIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLD-QSPRCLR 189
           W++T +A    SM IV  YDTLG       + ++ +  +  E      L+   +    L 
Sbjct: 134 WLLTAEACLSQSMTIVTAYDTLGEEGLLHSLRESGVRGMYTEGHLLKTLVNPLKEIESLE 193

Query: 190 KLI---TIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTXGT 360
            +I     KE    T Q  +   ++++KF+D E      +    PP PE +C I YT G+
Sbjct: 194 VIIYRNDAKEEDIKTIQEIRPN-LKLIKFADFEKMSPPVEPD--PPSPEEICCIMYTSGS 250

Query: 361 TGMPKGVMLTH 393
           TG+PKGV+L+H
Sbjct: 251 TGLPKGVILSH 261


>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 61.7 bits (143), Expect = 4e-11
 Identities = 38/131 (29%), Positives = 61/131 (46%), Gaps = 3/131 (2%)
 Frame = +1

Query: 10  EWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQSPRCLR 189
           +W  T Q     ++ IV  Y+TLG +     +++ +   +  + +    LL         
Sbjct: 137 KWFTTAQGCSSQAIPIVTAYETLGEDGIYTSLDECKSRAIFTDPNLIPKLLGPLKQSTWV 196

Query: 190 KLITIKEV-SPSTFQRAKSRG--VEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTXGT 360
           KLI      S    +  KS    VEI+ + ++   G +K  P  PPK +++C   YT G+
Sbjct: 197 KLIVCSSTPSEDLVELVKSTAPDVEIITYDNLLSLGKEKPQPPHPPKADDICCYMYTSGS 256

Query: 361 TGMPKGVMLTH 393
           TG PKGV+L H
Sbjct: 257 TGKPKGVVLLH 267


>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 512

 Score = 41.1 bits (92), Expect = 6e-05
 Identities = 22/52 (42%), Positives = 30/52 (57%)
 Frame = +1

Query: 238 KSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTXGTTGMPKGVMLTH 393
           KSR V I+ F   +I   Q   P   P+P+++  + +T GTTG PK V LTH
Sbjct: 135 KSRLVRIVHFEGAKINAPQ---PLGLPQPDDVMLVLHTSGTTGRPKVVPLTH 183


>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 662

 Score = 37.5 bits (83), Expect = 7e-04
 Identities = 38/134 (28%), Positives = 58/134 (43%), Gaps = 8/134 (5%)
 Frame = +1

Query: 7   PEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDK----KANLLLDQS 174
           PE I+   A      +   ++    A + A  VN +E  V+I  D+     K   L    
Sbjct: 153 PETIIAMLAIVRLGAIHSVIFAGFSAESVADRVNDSECKVIITADESHRGGKRIPLKGVV 212

Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHP-FVPP---KPENLCTI 342
            + L +  TIK+V    FQR+      +++  DV         P + PP    PE+   +
Sbjct: 213 NKALTECPTIKKVL--VFQRSAEPTASMVEGRDVWWHDIIPKFPRYCPPAVVNPEHPLFL 270

Query: 343 CYTXGTTGMPKGVM 384
            YT G+TG PKGV+
Sbjct: 271 LYTSGSTGKPKGVV 284


>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 4924

 Score = 35.1 bits (77), Expect = 0.004
 Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = +1

Query: 241  SRGVEILKFSDVEIQGAQKDHPFVPP-KPENLCTICYTXGTTGMPKGVMLTH 393
            S  V IL  SD        ++P+    +  NL  + YT G+TG PKG  LTH
Sbjct: 2841 SINVTILDASDPGNYSNNIENPYTKDFEDSNLAYVLYTSGSTGKPKGCCLTH 2892



 Score = 31.9 bits (69), Expect = 0.037
 Identities = 15/41 (36%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
 Frame = +1

Query: 274 VEIQGAQKDHPF-VPPKPENLCTICYTXGTTGMPKGVMLTH 393
           +EI+   +  PF  P   +++  + YT G+TG PKGV ++H
Sbjct: 341 MEIKVDDEIPPFPFPESLDSVAYVLYTSGSTGNPKGVAISH 381


>SPBC146.03c |cut3|smc4, smc4|condensin subunit
           Cut3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1324

 Score = 27.5 bits (58), Expect = 0.80
 Identities = 13/29 (44%), Positives = 18/29 (62%)
 Frame = +2

Query: 263 SSLTSKYRELKRTILLSHRNRKTFVQYVI 349
           + L  K  +L RTIL   RN+KT VQ ++
Sbjct: 351 NELFMKQNQLYRTILYETRNKKTLVQNLL 379


>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1419

 Score = 26.6 bits (56), Expect = 1.4
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +1

Query: 322 PENLCTICYTXGTTGMPKGVMLTH 393
           P++  T+ +T G+ G+PKGV   H
Sbjct: 456 PDSTPTLSFTSGSEGIPKGVKGRH 479


>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
           E3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 26.2 bits (55), Expect = 1.8
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = +1

Query: 331 LCTICYTXGTTGMPKGVMLT 390
           LC +C+T   +G P+G++LT
Sbjct: 668 LCDLCHTTVQSGDPEGLLLT 687


>SPBC29A3.02c |his7||phosphoribosyl-AMP
           cyclohydrolase/phosphoribosyl-ATP pyrophosphohydrolase
           His7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 417

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 15/45 (33%), Positives = 24/45 (53%)
 Frame = +1

Query: 52  VIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQSPRCL 186
           VIV     LG  AC  ++N   +A+++  +++  N L D SP  L
Sbjct: 54  VIVDTTAELGPEACVNLLNAGALAILV--NEEMLNELADISPNRL 96


>SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 764

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 13/47 (27%), Positives = 25/47 (53%), Gaps = 4/47 (8%)
 Frame = +1

Query: 193 LITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPF----VPPK 321
           L T K++    ++  K++GV+I+  +D  +     DH F    +PP+
Sbjct: 36  LATAKQLRLEHWKLQKAQGVDIIPSNDFSLYDQIMDHSFSFNVIPPR 82


>SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 598

 Score = 24.6 bits (51), Expect = 5.6
 Identities = 13/63 (20%), Positives = 29/63 (46%)
 Frame = +1

Query: 142 DKKANLLLDQSPRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPK 321
           D+K   L + S + L ++  +K +     +   ++ +EI    ++++     D  F P K
Sbjct: 377 DRKKERLEEASQKRLEEVNRLKNLKRKELEEKLNQVIEIAGSKNIDVSKLDLDEDFDPEK 436

Query: 322 PEN 330
            E+
Sbjct: 437 WES 439


>SPCC1919.02 |||pig-X|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 332

 Score = 24.2 bits (50), Expect = 7.4
 Identities = 8/23 (34%), Positives = 16/23 (69%)
 Frame = +2

Query: 266 SLTSKYRELKRTILLSHRNRKTF 334
           S+  ++RE+K  I +SH +R+ +
Sbjct: 43  SIAKQFREIKNNINISHLSRENY 65


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
           Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 23.8 bits (49), Expect = 9.8
 Identities = 9/24 (37%), Positives = 13/24 (54%)
 Frame = -3

Query: 375 FRHPSRSXRITYCTKVFRFRWDKR 304
           FR      + +YC+ +  FRW KR
Sbjct: 391 FRDAEDEYKSSYCSHMDAFRWVKR 414


>SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 313

 Score = 23.8 bits (49), Expect = 9.8
 Identities = 9/13 (69%), Positives = 10/13 (76%)
 Frame = +3

Query: 321 TGKPLYNMLYVRN 359
           TG PLYN  Y+RN
Sbjct: 91  TGDPLYNSPYLRN 103


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,674,744
Number of Sequences: 5004
Number of extensions: 31704
Number of successful extensions: 100
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 132093910
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)