Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0060
(395 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27600.1 68418.m03305 AMP-binding protein, putative similar t... 110 3e-25
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon... 108 2e-24
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil... 102 8e-23
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 94 3e-20
At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta... 93 6e-20
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 91 3e-19
At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase, puta... 88 2e-18
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 68 2e-12
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 68 2e-12
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 67 3e-12
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t... 42 1e-04
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 41 3e-04
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 40 8e-04
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam... 39 0.001
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 39 0.001
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 38 0.002
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 38 0.002
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 37 0.004
At4g14070.1 68417.m02172 AMP-binding protein, putative similar t... 36 0.007
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 35 0.023
At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident... 34 0.039
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 33 0.052
At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) simil... 31 0.21
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 31 0.21
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 31 0.21
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 30 0.64
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 30 0.64
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 29 0.85
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 29 1.1
At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 29 1.1
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 29 1.5
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 28 2.0
At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase fam... 28 2.0
At4g30930.1 68417.m04391 50S ribosomal protein L21, mitochondria... 28 2.6
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 28 2.6
At2g22475.1 68415.m02666 GRAM domain-containing protein / ABA-re... 27 3.4
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 27 3.4
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 27 3.4
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil... 27 3.4
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 27 3.4
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 27 3.4
At4g21400.1 68417.m03091 protein kinase family protein contains ... 27 4.5
At5g16370.1 68418.m01913 AMP-binding protein, putative similar t... 27 6.0
At3g30670.1 68416.m03887 hypothetical protein 27 6.0
At2g33270.1 68415.m04078 thioredoxin family protein contains Pfa... 27 6.0
At1g40125.1 68414.m04763 hypothetical protein member of hypothet... 27 6.0
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 26 7.9
At4g17250.1 68417.m02594 expressed protein 26 7.9
At3g62230.1 68416.m06991 F-box family protein contains Pfam:PF00... 26 7.9
At2g43490.1 68415.m05404 RabGAP/TBC domain-containing protein si... 26 7.9
>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, long-chain-fatty-acid--CoA ligase - Brassica
napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
domain PF00501
Length = 700
Score = 110 bits (265), Expect = 3e-25
Identities = 55/133 (41%), Positives = 74/133 (55%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLL--LDQS 174
N PEW++ + A YS V VPLYDTLG +A F+VN + + C LL L +
Sbjct: 152 NRPEWLVVDHACAAYSFVSVPLYDTLGPDAVKFVVNHANLQAIFCVPQTLNILLSFLAEI 211
Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTX 354
P +R ++ + + GV I+ + + QG HPF PPKPE++ TICYT
Sbjct: 212 PS-IRLIVVVGGADEHLPSLPRGTGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTS 270
Query: 355 GTTGMPKGVMLTH 393
GTTG PKGV+LTH
Sbjct: 271 GTTGTPKGVVLTH 283
>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase (LACS6) strong similarity
to AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, similar to putative long-chain-fatty-acid--CoA
ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA AtLACS6 for long-chain acyl-CoA
synthetase GI:22531705
Length = 701
Score = 108 bits (259), Expect = 2e-24
Identities = 55/133 (41%), Positives = 78/133 (58%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLL--LDQS 174
N PEW++ + A YS V VPLYDTLG +A FIVN + + C + +LL L +
Sbjct: 152 NRPEWLIVDHACSSYSYVSVPLYDTLGPDAVKFIVNHATVQAIFCVAETLNSLLSCLSEM 211
Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTX 354
P +R ++ + + S S GV+++ +S + QG F PPKP+++ TICYT
Sbjct: 212 PS-VRLVVVVGGLIESLPSLPSSSGVKVVSYSVLLNQGRSNPQRFFPPKPDDVATICYTS 270
Query: 355 GTTGMPKGVMLTH 393
GTTG PKGV+LTH
Sbjct: 271 GTTGTPKGVVLTH 283
>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
[Brassica napus] ; contains Pfam AMP-binding enzyme
domain PF00501
Length = 660
Score = 102 bits (245), Expect = 8e-23
Identities = 52/134 (38%), Positives = 76/134 (56%), Gaps = 3/134 (2%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQSPR 180
NCP+WI+ +A ++++ VPLYDTLG+ A +IV E+ V +D K LL + +
Sbjct: 110 NCPQWIIAMEACAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLL-EPDCK 168
Query: 181 CLRKL---ITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYT 351
C ++L ++ VS +A GV+ + D G +K PPK N+CTI YT
Sbjct: 169 CAKRLKAIVSFTNVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYT 228
Query: 352 XGTTGMPKGVMLTH 393
GT+G PKGV+LTH
Sbjct: 229 SGTSGDPKGVVLTH 242
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 93.9 bits (223), Expect = 3e-20
Identities = 45/133 (33%), Positives = 77/133 (57%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQS-- 174
NCPEWI+ +A + VPLYD+LG NA FI+N E+++V ++ +++L Q
Sbjct: 113 NCPEWIIAMEACMSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGC 172
Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTX 354
L+ +++ EVS + + AK++ V + +++ + G + + ++CTI YT
Sbjct: 173 SSNLKTIVSFGEVSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTS 232
Query: 355 GTTGMPKGVMLTH 393
GTTG PKGV+L +
Sbjct: 233 GTTGEPKGVILNN 245
>At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to acyl-CoA synthetase (MF7P) gi:1617270 from
Brassica napus
Length = 666
Score = 93.1 bits (221), Expect = 6e-20
Identities = 46/133 (34%), Positives = 74/133 (55%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLL--LDQS 174
NC EWI++ +A + + VPLYDTLGA A FI++ E+++ E+ K L S
Sbjct: 113 NCCEWIISMEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNS 172
Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTX 354
+ ++ +++ V P + A+ G+ I + + G K + KP ++CTI YT
Sbjct: 173 TKYMKTVVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTS 232
Query: 355 GTTGMPKGVMLTH 393
GTTG PKGVM+++
Sbjct: 233 GTTGDPKGVMISN 245
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 90.6 bits (215), Expect = 3e-19
Identities = 46/133 (34%), Positives = 77/133 (57%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLL--LDQS 174
N PEWI++ +A + + VPLYDTLGA+A FI++ +E+++V E+ K + L S
Sbjct: 113 NSPEWIISMEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNS 172
Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTX 354
++ +++ VS + A++ G+ I + + G K + K ++CTI YT
Sbjct: 173 TEYMKTVVSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTS 232
Query: 355 GTTGMPKGVMLTH 393
GTTG PKGVM+++
Sbjct: 233 GTTGDPKGVMISN 245
>At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to GI:1617270 (MF7P) from [Brassica napus]
Length = 665
Score = 87.8 bits (208), Expect = 2e-18
Identities = 46/133 (34%), Positives = 75/133 (56%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 NCPEWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLL--LDQS 174
N PEWI++ +A + + VPLYDTLGA A FI+ E+++ E++K + LL +S
Sbjct: 113 NSPEWIISMEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKS 172
Query: 175 PRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTX 354
+ L+ +++ EV+ + A+ + I + G K + + ++CTI YT
Sbjct: 173 TKYLKYIVSFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTS 232
Query: 355 GTTGMPKGVMLTH 393
GTTG PKGV+LT+
Sbjct: 233 GTTGDPKGVLLTN 245
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 68.1 bits (159), Expect = 2e-12
Identities = 37/131 (28%), Positives = 68/131 (51%), Gaps = 3/131 (2%)
Frame = +1
Query: 10 EWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQSP-RCL 186
EW + Q + S+ +V +Y +LG A + +N+T ++ +IC+ + L QS + +
Sbjct: 166 EWFIAFQGCFRQSITVVTIYASLGEEALIYSLNETRVSTLICDSKQLKKLSAIQSSLKTV 225
Query: 187 RKLITIKE--VSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTXGT 360
+ +I I+E V ++ + + S+VE G + + P + I +T G+
Sbjct: 226 KNIIYIEEDGVDVASSDVNSMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGS 285
Query: 361 TGMPKGVMLTH 393
TG+PKGVM+TH
Sbjct: 286 TGLPKGVMITH 296
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 68.1 bits (159), Expect = 2e-12
Identities = 37/131 (28%), Positives = 68/131 (51%), Gaps = 3/131 (2%)
Frame = +1
Query: 10 EWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQSP-RCL 186
EW + Q + S+ +V +Y +LG A + +N+T ++ +IC+ + L QS + +
Sbjct: 166 EWFIAFQGCFRQSITVVTIYASLGEEALIYSLNETRVSTLICDSKQLKKLSAIQSSLKTV 225
Query: 187 RKLITIKE--VSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPENLCTICYTXGT 360
+ +I I+E V ++ + + S+VE G + + P + I +T G+
Sbjct: 226 KNIIYIEEDGVDVASSDVNSMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGS 285
Query: 361 TGMPKGVMLTH 393
TG+PKGVM+TH
Sbjct: 286 TGLPKGVMITH 296
>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
HMM hit: AMP-binding enzymes PF00501
Length = 691
Score = 67.3 bits (157), Expect = 3e-12
Identities = 40/130 (30%), Positives = 68/130 (52%), Gaps = 2/130 (1%)
Frame = +1
Query: 10 EWIMTEQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDDKKANLLLDQSPRCLR 189
EW ++ Q + ++ +V +Y +LG A +N+TE+ VIC K+ L+D S +
Sbjct: 143 EWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVIC-GSKELKKLMDIS----Q 197
Query: 190 KLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVP--PKPENLCTICYTXGTT 363
+L T+K V + + S ++Q +++P P P ++ I YT G+T
Sbjct: 198 QLETVKRVICMDDEFPSDVNSNWMATSFTDVQKLGRENPVDPNFPLSADVAVIMYTSGST 257
Query: 364 GMPKGVMLTH 393
G+PKGVM+TH
Sbjct: 258 GLPKGVMMTH 267
>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
AMP-binding protein GB:CAA96521 from [Brassica napus]
(Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-CoA synthetase-like protein GI:20799732
Length = 722
Score = 42.3 bits (95), Expect = 1e-04
Identities = 17/24 (70%), Positives = 20/24 (83%)
Frame = +1
Query: 322 PENLCTICYTXGTTGMPKGVMLTH 393
P+++ TI YT GTTG PKGVMLTH
Sbjct: 262 PDDIATIMYTSGTTGNPKGVMLTH 285
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 40.7 bits (91), Expect = 3e-04
Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Frame = +1
Query: 214 SPSTFQRAKSRGVEILKFSDV-EIQGAQKDHPFVPPKPENLCTICYTXGTTGMPKGVMLT 390
S T + +IL F +V E+ ++PFV K + + Y+ GTTG KGV LT
Sbjct: 152 SKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELT 211
Query: 391 H 393
H
Sbjct: 212 H 212
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 39.5 bits (88), Expect = 8e-04
Identities = 20/47 (42%), Positives = 31/47 (65%), Gaps = 2/47 (4%)
Frame = +1
Query: 259 LKFSDVEIQGAQKDHPFVPPK--PENLCTICYTXGTTGMPKGVMLTH 393
L+FS++ Q + +P K PE++ + ++ GTTG+PKGVMLTH
Sbjct: 172 LRFSELT-QSEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTH 217
>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 7 AMPBP7 (AMPBP7) GI:20799722
Length = 569
Score = 39.1 bits (87), Expect = 0.001
Identities = 31/117 (26%), Positives = 59/117 (50%), Gaps = 11/117 (9%)
Frame = +1
Query: 76 LGANACAFIVNQTEMAVVICEDD------KKANLLLDQSPRCLRK--LITIKE--VSPST 225
L A AF+++ ++ +V++ + + L+ +++ ++ LI I + +P +
Sbjct: 106 LNAPTVAFLLSHSQSSVIMVDQEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPES 165
Query: 226 FQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPE-NLCTICYTXGTTGMPKGVMLTH 393
RA S+G +++ D G ++P+ PP E + YT GTT PKGV+L H
Sbjct: 166 LNRALSKGA--IEYEDFLATG-DPNYPWQPPADEWQSIALGYTSGTTASPKGVVLHH 219
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 38.7 bits (86), Expect = 0.001
Identities = 23/68 (33%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +1
Query: 193 LITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKPE-NLCTICYTXGTTGM 369
L+ I E + R K R E++++ DV G + D + P E + ++ YT GTT
Sbjct: 144 LVLIPEPLTQSVSR-KKRSEEMMEYEDVVAMG-KSDFEVIRPTDECDAISVNYTSGTTSS 201
Query: 370 PKGVMLTH 393
PKGV+ +H
Sbjct: 202 PKGVVYSH 209
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 37.9 bits (84), Expect = 0.002
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = +1
Query: 322 PENLCTICYTXGTTGMPKGVMLTH 393
P+++ + Y+ GTTG+PKGVMLTH
Sbjct: 201 PDDVVALPYSSGTTGLPKGVMLTH 224
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 37.9 bits (84), Expect = 0.002
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = +1
Query: 322 PENLCTICYTXGTTGMPKGVMLTH 393
P+++ + Y+ GTTG+PKGVMLTH
Sbjct: 201 PDDVVALPYSSGTTGLPKGVMLTH 224
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 37.1 bits (82), Expect = 0.004
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1
Query: 322 PENLCTICYTXGTTGMPKGVMLTH 393
PE+ + Y+ GTTG+PKGVM+TH
Sbjct: 209 PEDTVAMPYSSGTTGLPKGVMITH 232
>At4g14070.1 68417.m02172 AMP-binding protein, putative similar to
AMP-binding protein [gi:1617272] from Brassica napus;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA acyl-CoA synthetase-like protein
GI:20799730
Length = 727
Score = 36.3 bits (80), Expect = 0.007
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ I YT GTTG PKGVMLTH
Sbjct: 279 DDTAAIMYTSGTTGNPKGVMLTH 301
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 34.7 bits (76), Expect = 0.023
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +1
Query: 328 NLCTICYTXGTTGMPKGVMLTH 393
+LC + ++ GTTG+ KGVMLTH
Sbjct: 182 DLCALPFSSGTTGLQKGVMLTH 203
>At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14)
identical to acyl-activating enzyme 14 [Arabidopsis
thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase
1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1)
{Arabidopsis thaliana}; contains Pfam profile PF00501:
AMP-binding enzyme; identical to cDNA acyl-activating
enzyme 14 (At1g30520) GI:29893263
Length = 560
Score = 33.9 bits (74), Expect = 0.039
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ IC+T GTTG PKGV ++H
Sbjct: 172 DDAVVICFTSGTTGRPKGVTISH 194
>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL
from Pinus taeda, gi:515503, gi:1143308; contains Pfam
AMP-binding enzyme domain PF00501
Length = 566
Score = 33.5 bits (73), Expect = 0.052
Identities = 16/32 (50%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = +1
Query: 307 FVPP---KPENLCTICYTXGTTGMPKGVMLTH 393
FVP K +++ I Y+ GTTG KGV+LTH
Sbjct: 195 FVPKPLIKQDDVAAIMYSSGTTGASKGVLLTH 226
>At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) similar
to malonyl CoA synthetase GB:AAF28840 from
[Bradyrhizobium japonicum]; contains Pfam AMP-binding
enzyme domain PF00501; identical to cDNA acyl-activating
enzyme 13 (At3g16170) GI:29893232, acyl-activating
enzyme 13 [Arabidopsis thaliana] GI:29893233
Length = 544
Score = 31.5 bits (68), Expect = 0.21
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = +1
Query: 340 ICYTXGTTGMPKGVMLTH 393
I YT GTTG PKGV+ TH
Sbjct: 175 IVYTSGTTGKPKGVVHTH 192
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 31.5 bits (68), Expect = 0.21
Identities = 11/23 (47%), Positives = 18/23 (78%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ + ++ GTTG+PKGV+LTH
Sbjct: 205 DDAAALPFSSGTTGLPKGVVLTH 227
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 31.5 bits (68), Expect = 0.21
Identities = 11/23 (47%), Positives = 18/23 (78%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ + ++ GTTG+PKGV+LTH
Sbjct: 205 DDAAALPFSSGTTGLPKGVVLTH 227
>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 473
Score = 29.9 bits (64), Expect = 0.64
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ T+ Y+ GTTGM KGV+ +H
Sbjct: 189 DDTATLLYSSGTTGMSKGVISSH 211
>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 546
Score = 29.9 bits (64), Expect = 0.64
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ T+ Y+ GTTGM KGV+ +H
Sbjct: 189 DDTATLLYSSGTTGMSKGVISSH 211
>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
Oryza sativa; contains Pfam AMP-binding enzyme domain
PF00501
Length = 565
Score = 29.5 bits (63), Expect = 0.85
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +1
Query: 325 ENLCTICYTXGTTGMPKGVMLTH 393
++ + Y+ GTTG KGVML+H
Sbjct: 213 DDTAALLYSSGTTGTSKGVMLSH 235
>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 9 AMPBP9 (AMPBP9) GI:20799726
Length = 550
Score = 29.1 bits (62), Expect = 1.1
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +1
Query: 346 YTXGTTGMPKGVMLTH 393
YT GTT PKGV+L+H
Sbjct: 194 YTSGTTSSPKGVVLSH 209
>At1g21530.1 68414.m02693 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 10 AMPBP10 (AMPBP10) GI:20799728
Length = 547
Score = 29.1 bits (62), Expect = 1.1
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +1
Query: 346 YTXGTTGMPKGVMLTH 393
YT GTT PKGV+L+H
Sbjct: 191 YTSGTTSSPKGVVLSH 206
>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 2 AMPBP2 (AMPBP2) GI:20799712
Length = 603
Score = 28.7 bits (61), Expect = 1.5
Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +1
Query: 280 IQGAQKDHPFVPPKPE-NLCTICYTXGTTGMPKGVMLTH 393
++ + + P+ E + +I YT GTT PKGV+ +H
Sbjct: 225 LKSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSH 263
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 28.3 bits (60), Expect = 2.0
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +1
Query: 301 HPF-VPPKPENLCTICYTXGTTGMPKGVMLTH 393
+PF V + I ++ GTTG KGV+LTH
Sbjct: 195 NPFQVQVNQSDPAAILFSSGTTGRVKGVLLTH 226
>At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase family
protein similar to iturin A synthetase C [Bacillus
subtilis] GI:16040972; contains Pfam profile PF00501:
AMP-binding enzyme
Length = 1040
Score = 28.3 bits (60), Expect = 2.0
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 304 PFVPPKPENLCTICYTXGTTGMPKGV 381
P + C + YT G+TG PKGV
Sbjct: 95 PCKKERQRKFCYLMYTSGSTGKPKGV 120
>At4g30930.1 68417.m04391 50S ribosomal protein L21, mitochondrial
(RPL21M) identical to SP|Q8L9A0 50S ribosomal protein
L21, mitochondrial precursor {Arabidopsis thaliana}
Length = 270
Score = 27.9 bits (59), Expect = 2.6
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +1
Query: 190 KLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPPKP 324
K++ K+ ++R + E+ K +IQG +K P + KP
Sbjct: 211 KVLIFKKKRRKNYRRTRGHRQELTKLRITDIQGIEKPEPKIVHKP 255
>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam profile: PF00501 AMP-binding enzyme;
identical to cDNA adenosine monophosphate binding
protein 8 AMPBP8 (AMPBP8) GI:20799724
Length = 544
Score = 27.9 bits (59), Expect = 2.6
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +1
Query: 265 FSDVEIQGAQKDHPFVPPKPE-NLCTICYTXGTTGMPKGVMLTH 393
+SD+ +G D ++ P E + + YT GTT PKGV+ H
Sbjct: 164 YSDLITRG-NPDFKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCH 206
>At2g22475.1 68415.m02666 GRAM domain-containing protein /
ABA-responsive protein-related similar to ABA-responsive
protein [Hordeum vulgare] GI:4103635; contains Pfam
profile PF02893: GRAM domain
Length = 299
Score = 27.5 bits (58), Expect = 3.4
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +1
Query: 25 EQAAYCYSMVIVPLYDTLGANACAFIVNQTEMAVVICEDD 144
+Q + Y V++PL+ N A IVN E + + D
Sbjct: 229 DQTEWSYYKVVIPLHQLKAVNPSASIVNPAEKYIQVISVD 268
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 27.5 bits (58), Expect = 3.4
Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +1
Query: 307 FVPPKPE-NLCTICYTXGTTGMPKGVMLTH 393
+V PK E + YT GTT PKGV+ +H
Sbjct: 178 WVRPKSEWTPMVLNYTSGTTSSPKGVVHSH 207
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 27.5 bits (58), Expect = 3.4
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 346 YTXGTTGMPKGVMLTH 393
YT GTT PKGV+++H
Sbjct: 193 YTSGTTADPKGVVISH 208
>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
acyl-activating enzyme 11 [Arabidopsis thaliana]
GI:29893231
Length = 572
Score = 27.5 bits (58), Expect = 3.4
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 346 YTXGTTGMPKGVMLTH 393
YT GTT PKGV+++H
Sbjct: 193 YTSGTTADPKGVVISH 208
>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 12 (At1g65890) mRNA
GI:29893228, acyl-activating enzyme 12 [Arabidopsis
thaliana] GI:29893229
Length = 578
Score = 27.5 bits (58), Expect = 3.4
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 346 YTXGTTGMPKGVMLTH 393
YT GTT PKGV+++H
Sbjct: 193 YTSGTTADPKGVVISH 208
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 27.5 bits (58), Expect = 3.4
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 346 YTXGTTGMPKGVMLTH 393
YT GTT PKGV+++H
Sbjct: 193 YTSGTTADPKGVVISH 208
>At4g21400.1 68417.m03091 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 711
Score = 27.1 bits (57), Expect = 4.5
Identities = 19/78 (24%), Positives = 33/78 (42%)
Frame = +1
Query: 139 DDKKANLLLDQSPRCLRKLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHPFVPP 318
DD++A LL RC R+ I + + PS ++S + + + +Q + P +
Sbjct: 599 DDEEAENLLSWVWRCWREDIILSVIDPSLTTGSRSEILRCIHIGLLCVQESPASRPTMDS 658
Query: 319 KPENLCTICYTXGTTGMP 372
L + YT T P
Sbjct: 659 VALMLNSYSYTLPTPSRP 676
>At5g16370.1 68418.m01913 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA adenosine monophosphate binding protein 5 AMPBP5
(AMPBP5) GI:20799718
Length = 552
Score = 26.6 bits (56), Expect = 6.0
Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = +1
Query: 298 DHPFVPPKPE-NLCTICYTXGTTGMPKGVMLTH 393
D ++ P+ E + + YT GTT PKGV+ H
Sbjct: 175 DFKWIRPESEWDPVVLNYTSGTTSAPKGVVHCH 207
>At3g30670.1 68416.m03887 hypothetical protein
Length = 133
Score = 26.6 bits (56), Expect = 6.0
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +3
Query: 129 NL*RRQKSQSPTGSVASMPQKTDYN*GSIAVYISTRQEP 245
N+ +R +P G +S+PQ+ D+ ++ V +S P
Sbjct: 18 NVTQRAGGSTPAGRPSSLPQQYDFTPAAVTVQVSASIPP 56
>At2g33270.1 68415.m04078 thioredoxin family protein contains Pfam
profile: PF00085 Thioredoxin
Length = 273
Score = 26.6 bits (56), Expect = 6.0
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -3
Query: 279 FDVRELQNLNTTALGALKCRRRYFLNCNQFSEASRRLIQ*EIG 151
FD+R+ ++ ++ +L C ++CN FS R LI E G
Sbjct: 19 FDLRDFAGISPKSISSLCCISPRLISCNHFS--PRTLISGENG 59
>At1g40125.1 68414.m04763 hypothetical protein member of
hypothetical protein common family
Length = 168
Score = 26.6 bits (56), Expect = 6.0
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +3
Query: 129 NL*RRQKSQSPTGSVASMPQKTDYN*GSIAVYISTRQEP 245
N+ +R +P G +S+PQ+ D+ + AV +S P
Sbjct: 17 NVTQRAGGSTPAGRPSSLPQQYDFTPAATAVQVSASIPP 55
>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
acetate-CoA ligase, putative similar to SP|P27550
(Escherichia coli) and gi:8439651 (Homo sapiens);
contains Pfam AMP-binding enzyme domain PF00501
Length = 693
Score = 26.2 bits (55), Expect = 7.9
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +1
Query: 340 ICYTXGTTGMPKGVMLT 390
+ YT G+TG PKGV+ T
Sbjct: 307 LLYTSGSTGKPKGVLHT 323
>At4g17250.1 68417.m02594 expressed protein
Length = 416
Score = 26.2 bits (55), Expect = 7.9
Identities = 12/44 (27%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = +2
Query: 47 RWL*---FRCMIR*ERTLVPSLLTKLKWL**FVKTTKKPISYWI 169
RWL FRC R +++L L + W+ + ++P+++W+
Sbjct: 294 RWLKAVNFRCPFRNKKSLKDELRVESYWVECLAEKKERPLNFWM 337
>At3g62230.1 68416.m06991 F-box family protein contains Pfam:PF00646
F-box domain
Length = 461
Score = 26.2 bits (55), Expect = 7.9
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 342 YCTKVFRFRWDKRMVLLSSLYFDVRELQNLNTTALGAL 229
Y KV+RF + L+ Y D RE N +A GA+
Sbjct: 251 YFGKVYRFEFASANKLMEEAYLDYREESRYNVSA-GAI 287
>At2g43490.1 68415.m05404 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 756
Score = 26.2 bits (55), Expect = 7.9
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = +1
Query: 142 DKKANLLLDQSPRCLR-KLITIKEVSPSTFQRAKSRGVEILKFSDVEIQGAQKDHP 306
DK A+L+ +Q K++ V PS++ + V+ L+ SDV + KD P
Sbjct: 286 DKNADLIREQRRSTPEIKVMHSDSVGPSSYTGRNAEIVDGLRISDVPEMASVKDTP 341
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,675,490
Number of Sequences: 28952
Number of extensions: 163871
Number of successful extensions: 531
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 511
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 521
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 565902384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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