Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0054
(747 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 110 1e-24
At5g62500.1 68418.m07844 microtubule-associated EB1 family prote... 101 7e-22
At3g47690.1 68416.m05194 microtubule-associated EB1 family prote... 99 4e-21
At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50... 30 1.4
At2g03200.1 68415.m00273 aspartyl protease family protein contai... 30 1.4
At1g12730.2 68414.m01478 cell division cycle protein-related con... 30 1.9
At1g12730.1 68414.m01477 cell division cycle protein-related con... 30 1.9
At2g25140.1 68415.m03007 heat shock protein 100, putative / HSP1... 29 3.3
At5g13560.1 68418.m01566 expressed protein weak similarity to SP... 29 4.3
At3g58430.1 68416.m06512 meprin and TRAF homology domain-contain... 29 4.3
At3g44690.1 68416.m04806 expressed protein 28 5.7
At1g03370.1 68414.m00316 C2 domain-containing protein / GRAM dom... 28 7.6
At4g10370.1 68417.m01702 DC1 domain-containing protein contains ... 27 10.0
At3g58440.1 68416.m06513 meprin and TRAF homology domain-contain... 27 10.0
At2g30500.1 68415.m03715 kinase interacting family protein simil... 27 10.0
>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profiles PF00307: Calponin homology (CH)
domain, PF03271: EB1 protein
Length = 329
Score = 110 bits (264), Expect = 1e-24
Identities = 75/220 (34%), Positives = 103/220 (46%), Gaps = 9/220 (4%)
Frame = +1
Query: 1 IQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAA-YDAVAQR 177
IQN+K+LQ F K+ + K + + KLVKGR DN EF+QW KK+ D+ GG Y A+ +R
Sbjct: 71 IQNYKVLQDVFNKLKITKHIEVSKLVKGRPLDNLEFMQWMKKYCDSVNGGQHNYHALERR 130
Query: 178 EGLPMGHGGSXXXXXXXXXXXXXXXXXXXXXXQTIGKA-------NT-TVRSPPVNLSRI 333
E G + + G NT T S N
Sbjct: 131 EASKGGKEATKRAAATQQSGKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHS 190
Query: 334 SQSAKGDSKVVDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIVQK 513
S+ + SK V + +I ELK +D LEKERDFYF KLRD+E++CQ + +H P+V
Sbjct: 191 SKPSAKQSKPVPAYDEKITELKLYIDSLEKERDFYFSKLRDVEILCQNPDTEH-LPLVGS 249
Query: 514 ILDILYATEDGFAPPEEIDGDNPHPPEEDEY*NKTIRHQR 633
I ILYA + E +P +E N Q+
Sbjct: 250 IKRILYAADGEDVGAAETQTLSPIAEGSEERRNSVTESQK 289
>At5g62500.1 68418.m07844 microtubule-associated EB1 family protein
similar to EBF3-S (Microtubule-associated protein) [Homo
sapiens] GI:12751131; contains Pfam profiles PF00307:
Calponin homology (CH) domain, PF03271: EB1 protein
Length = 293
Score = 101 bits (241), Expect = 7e-22
Identities = 66/188 (35%), Positives = 89/188 (47%)
Frame = +1
Query: 1 IQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAAYDAVAQRE 180
IQN+K++Q F K+ + K + +++LVKGR DN EFLQW K+F D+ GG + E
Sbjct: 71 IQNYKVMQEVFTKLKITKPLEVNRLVKGRPLDNLEFLQWLKRFCDSINGGIMNENYNPVE 130
Query: 181 GLPMGHGGSXXXXXXXXXXXXXXXXXXXXXXQTIGKANTTVRSPPVNLSRISQSAKGDSK 360
G T K P S S
Sbjct: 131 RRSRGGREKSVKGSSKISKSLQTNNMHHPPVATSNKP----AGPKQAKSHGIGGGSNSSA 186
Query: 361 VVDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIVQKILDILYATE 540
V L+ ++ +LK +VD LEKERDFYF KLRDIE++CQ E + PIV + ILYAT+
Sbjct: 187 EVQALSKEVEDLKVSVDLLEKERDFYFSKLRDIEILCQ-TPELDDLPIVVAVKKILYATD 245
Query: 541 DGFAPPEE 564
+ EE
Sbjct: 246 ANESVLEE 253
>At3g47690.1 68416.m05194 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profile PF03271: EB1 protein
Length = 276
Score = 98.7 bits (235), Expect = 4e-21
Identities = 68/183 (37%), Positives = 93/183 (50%), Gaps = 3/183 (1%)
Frame = +1
Query: 1 IQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAA---YDAVA 171
IQN+K+LQ F K+ + K + I++LVKGR DN EFLQW K+F D+ GG Y+ V
Sbjct: 71 IQNYKVLQDVFNKLKITKPLEINRLVKGRPLDNLEFLQWLKRFCDSINGGIMNENYNPVE 130
Query: 172 QREGLPMGHGGSXXXXXXXXXXXXXXXXXXXXXXQTIGKANTTVRSPPVNLSRISQSAKG 351
+R G QT + S V LS+ S
Sbjct: 131 RRS-----RNGKERSVKGSNKIPKSL--------QT-NNNHPPPNSSSVGLSKASGPKSA 176
Query: 352 DSKVVDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIVQKILDILY 531
+ V L+ ++ +LK + D LEKERDFYF KLRD+E++CQ E + PIV + ILY
Sbjct: 177 KAAEVQALSKELVDLKISTDLLEKERDFYFSKLRDVEILCQ-TPELDDLPIVVAVKKILY 235
Query: 532 ATE 540
AT+
Sbjct: 236 ATD 238
>At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50)
identical to DNA repair-recombination protein GI:7110148
from [Arabidopsis thaliana]
Length = 1316
Score = 30.3 bits (65), Expect = 1.4
Identities = 20/80 (25%), Positives = 34/80 (42%), Gaps = 5/80 (6%)
Frame = +1
Query: 340 SAKGDSKVVDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHN-----API 504
S +G +++ H+I L+ + L +ERD G D + + EQ N I
Sbjct: 499 SERGFESKIEQKQHEIYSLEHKIKTLNRERDVMAGDAEDRVKLSLKKTEQENLKKKHKKI 558
Query: 505 VQKILDILYATEDGFAPPEE 564
+ + D + G PPE+
Sbjct: 559 IDECKDRIRGVLKGRLPPEK 578
>At2g03200.1 68415.m00273 aspartyl protease family protein contains
Pfam domain, PF00026: eukaryotic aspartyl protease
Length = 461
Score = 30.3 bits (65), Expect = 1.4
Identities = 18/47 (38%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
Frame = -1
Query: 279 GLGSSCDFGDRGDGFLSGSGPRG-GRAPVPHGQTFSLRHCVICRTSI 142
G+G C + GDGF GSG G GR P+ C TSI
Sbjct: 213 GIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSI 259
>At1g12730.2 68414.m01478 cell division cycle protein-related
contains 10 transmembrane domains; similar to PIG-U
(GI:27372215) [Rattus norvegicus]; similar to Cell
division cycle protein 91-like 1 (CDC91-like 1 protein)
(PIG-U) (Swiss-Prot:Q9H490) [Homo sapiens]
Length = 390
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = -1
Query: 462 LYIPKLTEVEVSLFF*SIYCGFQFVYLMV*FIHNFRITLGT 340
L++ +LT++E S F I+CG+ L+ +HNF I GT
Sbjct: 308 LFVNELTDMEYSFF---IFCGYIGFSLLSPVMHNFWIWRGT 345
>At1g12730.1 68414.m01477 cell division cycle protein-related
contains 10 transmembrane domains; similar to PIG-U
(GI:27372215) [Rattus norvegicus]; similar to Cell
division cycle protein 91-like 1 (CDC91-like 1 protein)
(PIG-U) (Swiss-Prot:Q9H490) [Homo sapiens]
Length = 474
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = -1
Query: 462 LYIPKLTEVEVSLFF*SIYCGFQFVYLMV*FIHNFRITLGT 340
L++ +LT++E S F I+CG+ L+ +HNF I GT
Sbjct: 392 LFVNELTDMEYSFF---IFCGYIGFSLLSPVMHNFWIWRGT 429
>At2g25140.1 68415.m03007 heat shock protein 100, putative / HSP100,
putative / heat shock protein clpB, putative /
HSP100/ClpB, putative similar to HSP100/ClpB GI:9651530
[Phaseolus lunatus]
Length = 964
Score = 29.1 bits (62), Expect = 3.3
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1
Query: 210 GRAPVPHGQTFSLRHCVICRTSIVGVKEFLEPLQ 109
GR G+T S ++CV+ TS +G LE L+
Sbjct: 782 GRITDSQGRTVSFKNCVVIMTSNIGSHHILETLR 815
>At5g13560.1 68418.m01566 expressed protein weak similarity to
SP|O42184 Restin (Cytoplasmic linker protein-170)
(CLIP-170) {Gallus gallus}
Length = 679
Score = 28.7 bits (61), Expect = 4.3
Identities = 25/112 (22%), Positives = 54/112 (48%), Gaps = 1/112 (0%)
Frame = +1
Query: 370 ELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIVQKILDILYATEDGF 549
+ +H + K T+D LEK D++ D+ + + ++ AP++ +I + + ++
Sbjct: 439 QCSHIETKDKQTLDELEKHEDYF----SDVALNILSVYKKEVAPLISRIENYVENLKN-L 493
Query: 550 APPEEIDGDNPHPPEEDEY*NKTIRHQRVS*CSCILYQTKLY-RLTIIDNIE 702
P E PP D+ N+ +++ I Y+TK+ +I+DN++
Sbjct: 494 GPGSE------KPPNADQGDNQVSNPRKILEEEYIDYETKIITTFSIVDNVK 539
>At3g58430.1 68416.m06512 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains weak hit to Pfam PF00917: MATH
domain
Length = 552
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +1
Query: 457 IEVICQEMEEQHNAPIVQKILDILYATEDGF 549
IE +CQ +EE N +V+ + + Y + GF
Sbjct: 391 IETLCQSLEELSNEDLVEADIALTYVKDAGF 421
>At3g44690.1 68416.m04806 expressed protein
Length = 1176
Score = 28.3 bits (60), Expect = 5.7
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +1
Query: 355 SKVVDELNHQINELKATVDGLEKERDFY 438
S +D ++H+++E K +DG+ ++ D Y
Sbjct: 41 SDKIDMVSHRVDEQKHDIDGIRQDSDLY 68
>At1g03370.1 68414.m00316 C2 domain-containing protein / GRAM
domain-containing protein contains Pfam profiles
PF00168: C2 domain; contains PF02893: GRAM domain;
similar to Chain A, Crystal Structure Of Synaptotagmin
Iii C2aC2B Length(GI:6980525); similar to Synaptotagmin
III (SytIII) (Swiss-Prot:P40748) [Rattus norvegicus]
Length = 1859
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 553 PPEEIDGDNPHPPEEDEY*NK 615
PP+ D DNP PP+ D N+
Sbjct: 238 PPKSSDPDNPKPPQSDPQSNR 258
>At4g10370.1 68417.m01702 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 687
Score = 27.5 bits (58), Expect = 10.0
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -3
Query: 538 QWHIKYQESFAL*EHCVVLPSLDRSPLYP 452
Q++ + F L EHC P + R PL+P
Sbjct: 394 QFYSCIESDFILHEHCANAPRMKRHPLHP 422
>At3g58440.1 68416.m06513 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 601
Score = 27.5 bits (58), Expect = 10.0
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +1
Query: 457 IEVICQEMEEQHNAPIVQKILDILYATEDGF 549
IE +CQ +EE N +V+ + + Y + GF
Sbjct: 342 IETMCQSLEELSNEDLVEADVALTYLRDAGF 372
>At2g30500.1 68415.m03715 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 517
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/52 (23%), Positives = 24/52 (46%)
Frame = +1
Query: 343 AKGDSKVVDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNA 498
A+ K+ D +NH + ++ D K+ YF K ++ + +E + A
Sbjct: 38 AENLEKMDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRA 89
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,472,863
Number of Sequences: 28952
Number of extensions: 321578
Number of successful extensions: 1011
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 965
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1009
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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