BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Nnor0009 (578 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 253 2e-68 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 147 1e-36 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 133 2e-32 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 120 1e-28 SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 29 0.65 SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 26 3.5 SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 26 3.5 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 26 3.5 SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|c... 26 4.6 SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 26 4.6 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 4.6 SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 25 6.1 SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|c... 25 6.1 SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 25 8.0 SPCC576.19c |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 25 8.0 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 253 bits (619), Expect = 2e-68 Identities = 111/183 (60%), Positives = 144/183 (78%) Frame = +3 Query: 30 MPSEMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRKDVFFYQADDDHY 209 M E+ITLQ GQCGNQIG +FW+QLC+EHGI P+G LE FA+ G DRKDVFFYQ+DD Y Sbjct: 1 MGREIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRY 60 Query: 210 IPRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASGYAQGEKLNEEVFD 389 IPRA+L+DLEPRV++ I++ Y LYNPEN+ ++K+GGGAGNNWA+GY+ E++ E++ D Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMD 120 Query: 390 IINREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFS*ETCSDIQCLPQT*MK*S 569 +I+READGSD+LEGF L HSIAGGTGSG+GS++LE L+DR+ + P + S Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNS-QSVS 179 Query: 570 DVV 578 DVV Sbjct: 180 DVV 182 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 147 bits (356), Expect = 1e-36 Identities = 65/159 (40%), Positives = 99/159 (62%), Gaps = 1/159 (0%) Frame = +3 Query: 39 EMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRKDVFFYQADDDHYIPR 218 E++ +Q GQCGNQ+G FW + EHG+ GI + A +R +V+F +A Y+PR Sbjct: 3 EIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPR 62 Query: 219 AVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFDII 395 AVL+DLEP + + + + L+ P+N+ + GAGN WA G Y +G +L + V D++ Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQ--SGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 396 NREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRF 512 REA+ D L+GF L HS+ GGTGSGMG+ +L + + + Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEY 159 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 133 bits (321), Expect = 2e-32 Identities = 68/162 (41%), Positives = 96/162 (59%), Gaps = 3/162 (1%) Frame = +3 Query: 39 EMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILE-EFASAGSDRK-DVFFYQADDDHYI 212 E+I++ +GQ G QIG W+ C+EHGI P G + E AS SD FF + Y+ Sbjct: 3 EIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYV 62 Query: 213 PRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFD 389 PR++ +DLEP VI + PY L++PE + K A NN+A G Y G++L +EV D Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKED--ASNNYARGHYTVGKELVDEVTD 120 Query: 390 IINREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLSDRFS 515 I R AD L+GF++ HS GGTGSG G+ +LE L+ ++ Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYT 162 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 120 bits (290), Expect = 1e-28 Identities = 61/162 (37%), Positives = 93/162 (57%), Gaps = 7/162 (4%) Frame = +3 Query: 39 EMITLQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAG------SDRKDVFFYQADD 200 E+I++ +GQ G QIG W+ C+EHGI P+G E + +D FF + Sbjct: 3 EVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQ 62 Query: 201 DHYIPRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNE 377 ++PR++ +DLEP VI + PY L++PE + K A NN+A G Y G+++ + Sbjct: 63 GKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKED--ASNNYARGHYTVGKEMID 120 Query: 378 EVFDIINREADGSDNLEGFVLCHSIAGGTGSGMGSYILEHLS 503 V + I R AD L+GF++ HS GGTGSG+G+ +LE L+ Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLN 162 >SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1260 Score = 28.7 bits (61), Expect = 0.65 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +3 Query: 408 DGSDNLEGFVLCHSIAGGTGSG 473 DG + EGF + +IAGG G+G Sbjct: 1104 DGENGEEGFAMYETIAGGAGAG 1125 >SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 26.2 bits (55), Expect = 3.5 Identities = 9/30 (30%), Positives = 18/30 (60%) Frame = -3 Query: 246 HAAPDPAELLVVCSDHHQLDRKTHLYDQIP 157 ++ P+PAE+ +C+DH + + L+ P Sbjct: 530 YSTPNPAEVQNICNDHAKFETSKSLHLSSP 559 >SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 26.2 bits (55), Expect = 3.5 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = +3 Query: 51 LQLGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRK 173 LQ+ Q F W+ C++ G +P+ L+ F + D K Sbjct: 18 LQICNMDEQTMFYKWESWCLQRGNTPKLDLDTFKAFAKDMK 58 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 26.2 bits (55), Expect = 3.5 Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = +3 Query: 120 ISP-EGILEEFASAGSDRKDVFFYQAD 197 ISP EGI+ E A+ D D+F Y D Sbjct: 1029 ISPVEGIIPEDANVRFDNVDIFLYDCD 1055 >SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|chr 3|||Manual Length = 479 Score = 25.8 bits (54), Expect = 4.6 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = +1 Query: 475 WAHIYWNTCLIGFPKKLVQTYSVF 546 W H+ W C++ F ++ Y VF Sbjct: 59 WRHLNWLHCMLIFGLPMIAIYGVF 82 >SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizosaccharomyces pombe|chr 2|||Manual Length = 1706 Score = 25.8 bits (54), Expect = 4.6 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +3 Query: 213 PRAVLLDLEPRVIHTIMNSPYAKLYNPENVYLS 311 P A ++ EP + SP+A ++NP NV S Sbjct: 1588 PSAGSVEREPLFLIAASGSPHAFIWNPHNVSAS 1620 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 4.6 Identities = 17/74 (22%), Positives = 34/74 (45%) Frame = +3 Query: 57 LGQCGNQIGFEFWKQLCIEHGISPEGILEEFASAGSDRKDVFFYQADDDHYIPRAVLLDL 236 LG G+ WK++ + ++ G++EEF + +D Y H + R + + L Sbjct: 958 LGAFGDSSDIRDWKKISSQRRVTQLGVVEEF-DILLELRDKTLYA----HKLSRIIEMGL 1012 Query: 237 EPRVIHTIMNSPYA 278 I ++ +P+A Sbjct: 1013 IESKIAVVIGTPHA 1026 >SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +3 Query: 99 QLCIEHGISPEGILEEFASAGSDRKDVFFYQAD 197 QL +E G S G+L + ++ D++F AD Sbjct: 197 QLVMELGQSKNGVLGTVSKTANEGPDIYFSNAD 229 >SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 571 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = +3 Query: 444 HSIAGGTGSGMGSYILEHLSDRF 512 + + GGT SG+GS L+ L+ RF Sbjct: 345 NKVEGGTRSGIGSGFLKRLALRF 367 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 25.0 bits (52), Expect = 8.0 Identities = 8/28 (28%), Positives = 16/28 (57%) Frame = +1 Query: 157 RDLIVKMCFSIKLMMITTYHEQFCWIWS 240 R L+ +C + + + T+H ++ WI S Sbjct: 85 RKLLFSLCGHMDYVRVCTFHHEYPWILS 112 >SPCC576.19c |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 119 Score = 25.0 bits (52), Expect = 8.0 Identities = 11/34 (32%), Positives = 16/34 (47%) Frame = -1 Query: 260 NRVYDTRLQIQQNCSWYVVIIISLIEKHIFTIRS 159 N +Y TRL +Q W++ I + L RS Sbjct: 6 NDLYQTRLDVQTPNRWFLYISLELFNNMYSPFRS 39 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,465,808 Number of Sequences: 5004 Number of extensions: 51666 Number of successful extensions: 157 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 248115846 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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