BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120953.seq (628 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 31 0.14 SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalyti... 26 3.9 SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch... 26 3.9 SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subuni... 26 3.9 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.8 SPAC1687.15 |gsk3|skp1|serine/threonine protein kinase Gsk3|Schi... 25 6.8 SPAC13G7.11 |||mitochondrial inner membrane protein|Schizosaccha... 25 9.0 >SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual Length = 978 Score = 31.1 bits (67), Expect = 0.14 Identities = 11/37 (29%), Positives = 25/37 (67%) Frame = +1 Query: 280 MEFVKLQCNICFSVAEIKNYFMQPIDRLTIIPVLELD 390 ++ +L+C C ++ EI N M+P+++L ++ +L L+ Sbjct: 240 LQIEELRCAACETMTEIVNKKMKPLEKLNLLNILNLN 276 >SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalytic subunit Cdc6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1086 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/36 (30%), Positives = 23/36 (63%) Frame = +1 Query: 283 EFVKLQCNICFSVAEIKNYFMQPIDRLTIIPVLELD 390 E +KL+ ++ +SV +YF++P R ++P++ D Sbjct: 613 ELLKLKQDVDYSVTPNGDYFVKPHVRKGLLPIILAD 648 >SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 593 Score = 26.2 bits (55), Expect = 3.9 Identities = 19/54 (35%), Positives = 25/54 (46%) Frame = -3 Query: 314 KQMLHCNLTNSIFRRLHLRPFAENNTRRRTCQCADAAAQSSVRICS*YKVVSQK 153 K+ N T+S R H RPF +RRR + A + S S Y+ SQK Sbjct: 69 KRTFPLNDTHSSRARQHERPFRSRKSRRRKGKKAFSPRPGSPPSPSFYRSGSQK 122 >SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subunit Rad15|Schizosaccharomyces pombe|chr 1|||Manual Length = 772 Score = 26.2 bits (55), Expect = 3.9 Identities = 12/46 (26%), Positives = 23/46 (50%) Frame = +1 Query: 280 MEFVKLQCNICFSVAEIKNYFMQPIDRLTIIPVLELDTCKHQLCSM 417 +E++K + + +AE F+Q + +T I L C +L S+ Sbjct: 336 VEYLKTRMKVLHVIAETPTSFLQHVKDITFIDKKPLRFCAERLTSL 381 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 25.4 bits (53), Expect = 6.8 Identities = 10/33 (30%), Positives = 12/33 (36%) Frame = +1 Query: 382 ELDTCKHQLCSMCXXXXXXXXXXPCPLCRVESL 480 +L CKH C CPLCR + Sbjct: 781 KLQACKHFFHQACIDQWLTTGNNSCPLCRAHGV 813 >SPAC1687.15 |gsk3|skp1|serine/threonine protein kinase Gsk3|Schizosaccharomyces pombe|chr 1|||Manual Length = 387 Score = 25.4 bits (53), Expect = 6.8 Identities = 20/94 (21%), Positives = 40/94 (42%), Gaps = 5/94 (5%) Frame = +3 Query: 354 RQIDYNTSIRTRHVQTSIMLNVHTQD*ETKKNTMSPVQG*KFAFQRLQ-----HKPNVVD 518 +Q+ Y +S +++ VH + ++K +Q +F + LQ PN+VD Sbjct: 28 KQLSYTSSKVVGSGSFGVVMQVHLIESDSKAAIKRVLQDKRFKNRELQIMRIMKHPNIVD 87 Query: 519 VIKCSVTSVAQWNKINXNFDAXFWASVLFEKSLL 620 +I T+ +++ N F ++ S L Sbjct: 88 LIAYYYTTGDNSDEVYLNLVLEFMPETIYRASRL 121 >SPAC13G7.11 |||mitochondrial inner membrane protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 269 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/45 (28%), Positives = 21/45 (46%) Frame = -2 Query: 354 YWLHKIIFNFCNRKTNVALQFDKLHF*AATPPPFRREQHQAQDLP 220 YW+++ NF R T V F + +F A P + + D+P Sbjct: 9 YWVNQFAINFACRNTYVNPMFLR-YFHCAVPRAISQRALKPMDIP 52 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,432,181 Number of Sequences: 5004 Number of extensions: 45784 Number of successful extensions: 113 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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