SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120917.seq
         (652 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo...    31   0.14 
SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo...    29   0.58 
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    28   1.3  
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9...    27   3.1  
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    27   3.1  
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz...    26   4.1  
SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T...    26   5.4  
SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces p...    26   5.4  
SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe...    25   7.2  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    25   7.2  
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos...    25   9.5  
SPCC1183.09c |pmp31|mug75|plasma membrane proteolipid Pmp31|Schi...    25   9.5  

>SPBC19G7.10c |||topoisomerase associated protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 744

 Score = 31.1 bits (67), Expect = 0.14
 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
 Frame = -2

Query: 468 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 289
           P+  +   L  L+S  A  R   T L +  A G +   +   PR++L V    EP+ + S
Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442

Query: 288 SID--SGFS 268
           S++  SGFS
Sbjct: 443 SLNNFSGFS 451


>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
           Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 385

 Score = 29.1 bits (62), Expect = 0.58
 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
 Frame = -1

Query: 427 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 314
           K+CG  A  HGH  R   R+W F   + Y  +K  ++  F
Sbjct: 39  KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 11/29 (37%), Positives = 17/29 (58%)
 Frame = -1

Query: 598  GICAIRAPLVRTAAPCWGRSSTQSCCRLR 512
            G+   +AP V + + C   S ++SCC LR
Sbjct: 1782 GLFENKAPFVNSTSICTAVSESRSCCHLR 1810


>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
            Srb9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1223

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = +2

Query: 287  DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 412
            D I + G NK    S+ + T+ +    SPN+ +T+SN   T+
Sbjct: 891  DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = -2

Query: 366 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSAIYICSIYYRRVRCA 223
           LTP    +P+ ++L+P L+   L  S+I SG     I     R VR A
Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSGSIHSDIRVSQLREVRAA 720


>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 322

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 14/52 (26%), Positives = 26/52 (50%)
 Frame = +2

Query: 290 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 445
           D +KE + K   +S+F    ++Y    PNA+    N T      ++D++E +
Sbjct: 64  DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112


>SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 988

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 16/51 (31%), Positives = 24/51 (47%)
 Frame = -1

Query: 256  LLNLLSPCAMRKLATRALLSFTFNRLFSSDSTDSLLCPKLGDASVKEFKFN 104
            LLN++     +KLA   +L +T  R  SS     L C  + D     FK++
Sbjct: 904  LLNVIGRKIWKKLAE--ILGYTSRRFLSSAEVKWLFCLGMRDGLKPSFKYH 952


>SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 718

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 12/38 (31%), Positives = 18/38 (47%)
 Frame = -1

Query: 577 PLVRTAAPCWGRSSTQSCCRLRIQHAIVLYSFSGRYTF 464
           PL  T +  WG     S C  ++ +   L+SF G+  F
Sbjct: 399 PLNETCSEWWGELLA-STCNTKLDNLSCLHSFDGKQNF 435


>SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 871

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 18/60 (30%), Positives = 28/60 (46%)
 Frame = +2

Query: 368 SPNAASTSSNVTMTRGTANFDIKEFKSMFIVFKGITPTKTVEDNGMLDSKSTA*LCA*ST 547
           S N+ ST S V      + FD  E  +   + K  +PT ++  N  L S+ T  + + ST
Sbjct: 407 SRNSKSTQSAVP--ENVSRFDSNEPSATSPILKRSSPTDSISQNSGLPSRLTEPISSPST 464


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = -2

Query: 414 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 313
           P V++    V  AF  +TP  YTVP+  +++PTL
Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415


>SPCC1322.12c |bub1||serine/threonine protein kinase
           Bub1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1044

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/43 (27%), Positives = 20/43 (46%)
 Frame = +2

Query: 263 IAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTS 391
           I  +PL  + + K   + +   S +LGT       S N+ +TS
Sbjct: 396 IFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENSSNSGATS 438


>SPCC1183.09c |pmp31|mug75|plasma membrane proteolipid
           Pmp31|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 105

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 9/27 (33%), Positives = 16/27 (59%)
 Frame = -3

Query: 281 IAVFRQYTFAQFIIAVCDAQIGHPGVI 201
           + + R +  A F+I +C   +G PG+I
Sbjct: 23  VGIRRGFCTADFLINICLCALGIPGII 49


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,715,381
Number of Sequences: 5004
Number of extensions: 55770
Number of successful extensions: 169
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -