BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120914.seq (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 28 1.0 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.8 SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 26 4.1 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.1 SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 26 4.1 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 25 7.2 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 7.2 SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.5 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 9.5 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 28.3 bits (60), Expect = 1.0 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = -3 Query: 586 FSVASSHNHFVGKQNERSVASAKSALPP 503 FS + SHNH Q E+S +++K ++ P Sbjct: 539 FSKSKSHNHHPSSQVEKSTSNSKGSMLP 566 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 27.5 bits (58), Expect = 1.8 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = +2 Query: 200 STSTASTNRRLSMPTKSSTCSR*RGKAGRPAVFAPTHSAHYQI 328 S+S+ S++R S T T S + + RP VF T +HY I Sbjct: 163 SSSSKSSSRSSSRTTSHRTTSH-KSSSYRPTVFPYTTISHYNI 204 >SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2176 Score = 26.2 bits (55), Expect = 4.1 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = -2 Query: 602 SCPSPFQRRVEPQSFRWQ 549 +CPS RRVE + F WQ Sbjct: 1172 NCPSEVIRRVEKKEFPWQ 1189 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 26.2 bits (55), Expect = 4.1 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = +2 Query: 179 IVNGQYVSTSTASTNRRLSMPTKSSTCS 262 I G Y STS +ST+ + P+ SST S Sbjct: 129 ISGGIYSSTSASSTSSSTATPSSSSTTS 156 >SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1428 Score = 26.2 bits (55), Expect = 4.1 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 2/30 (6%) Frame = -1 Query: 588 VSASRRATIISLANKMSDRL--LPPNLRCH 505 V+ RR + ISLAN++S L PP+ R H Sbjct: 683 VTEPRRISAISLANRVSQELGGNPPSARSH 712 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = -1 Query: 585 SASRRAT-IISLANKMSDRLLPPNLRCHRQFLRQIVNFGN 469 SASR+AT ++ ++SD LLP +L Q L + N + Sbjct: 591 SASRKATYLLGEVLRLSDELLPIHLGAKIQSLPSLFNMAS 630 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +1 Query: 118 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 243 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 25.0 bits (52), Expect = 9.5 Identities = 8/14 (57%), Positives = 11/14 (78%) Frame = -3 Query: 199 HVLPVHNLHILNCW 158 HVLP+ L +LNC+ Sbjct: 405 HVLPIRQLFLLNCY 418 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -3 Query: 241 RHAQTSICTCRRRGHVLPVHNLHILN 164 R A T + R +LPVH LH+ + Sbjct: 776 RQAMTRLKGLRETNSILPVHQLHMFH 801 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,604,593 Number of Sequences: 5004 Number of extensions: 53091 Number of successful extensions: 152 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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