BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120907.seq (648 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 31 0.14 SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo... 29 0.58 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 3.1 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 27 3.1 SPBC776.06c |||spindle pole body interacting protein |Schizosacc... 26 4.1 SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 26 4.1 SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T... 26 5.4 SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 25 7.1 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 25 7.1 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 9.4 SPCC1183.09c |pmp31|mug75|plasma membrane proteolipid Pmp31|Schi... 25 9.4 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.4 >SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 744 Score = 31.1 bits (67), Expect = 0.14 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Frame = -1 Query: 468 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 289 P+ + L L+S A R T L + A G + + PR++L V EP+ + S Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442 Query: 288 SID--SGFS 268 S++ SGFS Sbjct: 443 SLNNFSGFS 451 >SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 385 Score = 29.1 bits (62), Expect = 0.58 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%) Frame = -3 Query: 427 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 314 K+CG A HGH R R+W F + Y +K ++ F Sbjct: 39 KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 26.6 bits (56), Expect = 3.1 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +2 Query: 287 DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 412 D I + G NK S+ + T+ + SPN+ +T+SN T+ Sbjct: 891 DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 26.6 bits (56), Expect = 3.1 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = -1 Query: 366 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSAIYICSIYYRRVRCA 223 LTP +P+ ++L+P L+ L S+I SG I R VR A Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSGSIHSDIRVSQLREVRAA 720 >SPBC776.06c |||spindle pole body interacting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 26.2 bits (55), Expect = 4.1 Identities = 13/54 (24%), Positives = 28/54 (51%), Gaps = 9/54 (16%) Frame = +1 Query: 514 EVDSMIVCLIDPNTGPLFRTRGAR---------IAQIQLHSGVHEKRGSFSKFY 648 ++D ++ + DP+ GP+ + + +A++ L VHE+R +S F+ Sbjct: 2 QIDYLLTAIFDPDRGPVLQYQCPENGEASDLHFLAELMLPDRVHERREDWSLFF 55 >SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 26.2 bits (55), Expect = 4.1 Identities = 14/52 (26%), Positives = 26/52 (50%) Frame = +2 Query: 290 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 445 D +KE + K +S+F ++Y PNA+ N T ++D++E + Sbjct: 64 DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112 >SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 988 Score = 25.8 bits (54), Expect = 5.4 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = -3 Query: 256 LLNLLSPCAMRKLATRALLSFTFNRLFSSDSTDSLLCPKLGDASVKEFKFN 104 LLN++ +KLA +L +T R SS L C + D FK++ Sbjct: 904 LLNVIGRKIWKKLAE--ILGYTSRRFLSSAEVKWLFCLGMRDGLKPSFKYH 952 >SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 871 Score = 25.4 bits (53), Expect = 7.1 Identities = 18/60 (30%), Positives = 28/60 (46%) Frame = +2 Query: 368 SPNAASTSSNVTMTRGTANFDIKEFKSMFIVFKGITPTKTVEDNGMLDSKSTA*LCA*ST 547 S N+ ST S V + FD E + + K +PT ++ N L S+ T + + ST Sbjct: 407 SRNSKSTQSAVP--ENVSRFDSNEPSATSPILKRSSPTDSISQNSGLPSRLTEPISSPST 464 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/34 (41%), Positives = 21/34 (61%) Frame = -1 Query: 414 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 313 P V++ V AF +TP YTVP+ +++PTL Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.0 bits (52), Expect = 9.4 Identities = 12/43 (27%), Positives = 20/43 (46%) Frame = +2 Query: 263 IAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTS 391 I +PL + + K + + S +LGT S N+ +TS Sbjct: 396 IFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENSSNSGATS 438 >SPCC1183.09c |pmp31|mug75|plasma membrane proteolipid Pmp31|Schizosaccharomyces pombe|chr 3|||Manual Length = 105 Score = 25.0 bits (52), Expect = 9.4 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = -2 Query: 281 IAVFRQYTFAQFIIAVCDAQIGHPGVI 201 + + R + A F+I +C +G PG+I Sbjct: 23 VGIRRGFCTADFLINICLCALGIPGII 49 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 25.0 bits (52), Expect = 9.4 Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%) Frame = -1 Query: 630 ASFFVYTR--VQLDLRNSRTSRSEQRPRVGVDQAHNHAVDFESNMPLSSTVLVGVIPLNT 457 +SF Y + V +DLRNS R Q P V NH D + L S+ P +T Sbjct: 526 SSFSRYNQPTVSVDLRNSSILRRAQEP-VNGSTGENHIGDDYISSLLDSSNSNSQRPFST 584 Query: 456 INMDLN 439 + + N Sbjct: 585 VPSESN 590 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,699,645 Number of Sequences: 5004 Number of extensions: 55239 Number of successful extensions: 167 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 167 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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