BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120896.seq (638 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F12.07 |||phosphoserine aminotransferase |Schizosaccharomyc... 63 3e-11 SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Sc... 27 2.3 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 27 2.3 SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa... 27 2.3 SPCC1223.02 |nmt1|thi3|no message in thiamine Nmt1|Schizosacchar... 26 4.0 SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 7.0 SPBC887.16 |||dubious|Schizosaccharomyces pombe|chr 2|||Manual 25 7.0 >SPAC1F12.07 |||phosphoserine aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 389 Score = 63.3 bits (147), Expect = 3e-11 Identities = 42/135 (31%), Positives = 73/135 (54%), Gaps = 9/135 (6%) Frame = -3 Query: 636 YAGAQKNIGTSGVALVIVREDLLNQALPTMSLFIRL-------DS*LQTD--SILNTPPM 484 +AGAQKN G +G+ +V VR+ +L + P + + D + D S+ NT P+ Sbjct: 210 FAGAQKNAGPAGITVVFVRDSVLARPTPAELHKLNIPVSPTVSDYKIMADNHSLYNTLPV 269 Query: 483 FAIYIMGRVLQWIQRNGGLEGMSQLATKKASLIYNTIEQSNGFYYAPVAKNVRSKMNVPF 304 ++ + L+++ +GGL + + +K+ L+Y+T+++ + Y + V RS+MNV F Sbjct: 270 ATLHAINLGLEYMLEHGGLVALEASSIEKSKLLYDTLDK-HDLYISVVEPAARSRMNVTF 328 Query: 303 RIGCPGDDALEKEFL 259 RI LE EFL Sbjct: 329 RI---EPQELESEFL 340 Score = 52.8 bits (121), Expect = 4e-08 Identities = 23/49 (46%), Positives = 34/49 (69%) Frame = -2 Query: 277 LRKRILEGAETLGLIQLKGHRDVGGIRASIYNAVTLEEVQALVQYMKEF 131 L L AE L+QLKG+R VGGIRAS+YNA+++E+ + L+ ++ F Sbjct: 335 LESEFLAEAEKHHLVQLKGYRSVGGIRASLYNAISVEQTRRLIDLLESF 383 >SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Schizosaccharomyces pombe|chr 2|||Manual Length = 322 Score = 27.1 bits (57), Expect = 2.3 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +3 Query: 234 ISPKVSAPSRILFLRHHHQDNQF*MAHSFYCE 329 ++ K+ P+RI LR +H+ Q + FY E Sbjct: 114 VAMKLRYPNRITILRGNHESRQITQVYGFYDE 145 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = -3 Query: 321 KMNVPFRIGCPGDDALEKEFLKVLRLWDLFS*KDTETLAV 202 K NV ++G GD ++ K L V + F + T+TL V Sbjct: 6 KNNVTIKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGV 45 >SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +3 Query: 234 ISPKVSAPSRILFLRHHHQDNQF*MAHSFYCE 329 I+ K+ P RI LR +H+ Q + FY E Sbjct: 101 IAFKIRYPQRITILRGNHESRQITQVYGFYDE 132 >SPCC1223.02 |nmt1|thi3|no message in thiamine Nmt1|Schizosaccharomyces pombe|chr 3|||Manual Length = 346 Score = 26.2 bits (55), Expect = 4.0 Identities = 8/21 (38%), Positives = 16/21 (76%) Frame = +3 Query: 546 TWSVEPDSKDPL*Q*QALPQM 608 TW ++PD KDP+ + +A+ ++ Sbjct: 302 TWELDPDEKDPMGKQEAIAEI 322 >SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor complex subunit Rrn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 25.4 bits (53), Expect = 7.0 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = -2 Query: 304 QNWLSW**CLRKRILEGAETLGL 236 QNW+ W + +R+LEG+ G+ Sbjct: 150 QNWIDWEEKIAERLLEGSNISGV 172 >SPBC887.16 |||dubious|Schizosaccharomyces pombe|chr 2|||Manual Length = 109 Score = 25.4 bits (53), Expect = 7.0 Identities = 9/26 (34%), Positives = 17/26 (65%) Frame = -3 Query: 90 SNIYFLYVIWNLLFYIVVLIYVYN*N 13 SN++F ++ W L F + I++Y+ N Sbjct: 73 SNLHFFFLFWLLNFILFFRIHLYSCN 98 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,714,690 Number of Sequences: 5004 Number of extensions: 57940 Number of successful extensions: 142 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 140 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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