BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120894.seq (560 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g26570.1 68418.m03152 glycoside hydrolase starch-binding doma... 30 1.2 At1g67035.1 68414.m07623 expressed protein ; expression supporte... 29 2.8 At5g39540.1 68418.m04789 hypothetical protein predicted proteins... 27 8.6 At3g56790.1 68416.m06316 RNA splicing factor-related contains we... 27 8.6 At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 27 8.6 >At5g26570.1 68418.m03152 glycoside hydrolase starch-binding domain-containing protein similar to SEX1 (starch excess) [Arabidopsis thaliana] GI:12044358; contains Pfam profile PF00686: Starch binding domain Length = 1191 Score = 29.9 bits (64), Expect = 1.2 Identities = 16/53 (30%), Positives = 24/53 (45%) Frame = -3 Query: 183 WMVLARVSTKLVKAMLEE*GIKILHELGIYLIWVKLKLVTEIADGGFHEGSRY 25 W L V +V ++ E +K L IYL W+ + DGG H +R+ Sbjct: 263 WRKLEMVREVIVGSVEREERLKALIYSAIYLKWINTGQIPCFEDGGHHRPNRH 315 >At1g67035.1 68414.m07623 expressed protein ; expression supported by MPSS Length = 229 Score = 28.7 bits (61), Expect = 2.8 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -3 Query: 81 KLKLVTEIADGGFHEGSRYG 22 KLKL+ + D GF GSRYG Sbjct: 26 KLKLLVDNGDYGFERGSRYG 45 >At5g39540.1 68418.m04789 hypothetical protein predicted proteins, Arabidopsis thaliana Length = 196 Score = 27.1 bits (57), Expect = 8.6 Identities = 13/33 (39%), Positives = 16/33 (48%) Frame = +3 Query: 459 QAHDINNQQSQLVPGLLA*HGFHQFSRHSRQHH 557 Q HD+ QQ Q PG L + H S+Q H Sbjct: 160 QRHDLEYQQQQFCPGPLDSYQPHPHDMESQQPH 192 >At3g56790.1 68416.m06316 RNA splicing factor-related contains weak similarity to U4/U6-associated RNA splicing factor [Homo sapiens] gi|2853287|gb|AAC09069 Length = 206 Score = 27.1 bits (57), Expect = 8.6 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +3 Query: 3 QHHPPRRHNGSPRGNRHQQFPSQASTLPISGKFQ 104 +HH R H GS R +R ++ A T S +F+ Sbjct: 23 KHHHERSHEGSKRKDRSEEENGAARTTNKSSRFE 56 >At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong similarity to 60S ribosomal protein L1 GB:P49691 Length = 406 Score = 27.1 bits (57), Expect = 8.6 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +1 Query: 346 VDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKLMT 471 + ++ N I +DA A L + + N L+L PY +K M+ Sbjct: 304 IQSVVNPIKKDAKRAVLKKNPLKNLNVMLKLNPYAKTAKRMS 345 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,938,620 Number of Sequences: 28952 Number of extensions: 233348 Number of successful extensions: 557 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 402 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 557 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1072696904 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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