Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120889.seq
         (590 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   147   9e-37
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   135   4e-33
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    78   8e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    58   1e-09
SPBC649.03 |rhp14||XP-A family homolog Rhp14|Schizosaccharomyces...    27   1.5  
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces...    27   1.5  
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb...    25   8.3  
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch...    25   8.3  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  147 bits (357), Expect = 9e-37
 Identities = 67/115 (58%), Positives = 79/115 (68%)
 Frame = +1

Query: 133 GVQIGNACWELXCLEHGIPALMAXXPQTRPSGVETILSTLSQRDGAGKHVXRXVFVDLEP 312
           G QIGNACWEL CLEHGI       P+T     +   ST     G GK+V R ++VDLEP
Sbjct: 13  GTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEP 72

Query: 313 TVVDEVRTGTYRQLFHXEQXXTGKEXAGNKYARGHYTIGKEIVDLVLDRIRKLAD 477
            V+D+VRTG YR LFH EQ  TGKE A N YARGHYT+GKE+VD V D+IR++AD
Sbjct: 73  NVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127



 Score = 42.7 bits (96), Expect = 4e-05
 Identities = 19/36 (52%), Positives = 24/36 (66%)
 Frame = +2

Query: 482 CTGLQGFLISTPXGGGTGLWVHLLLMERFXVDYGKK 589
           C+GLQGFL+    GGGTG     LL+ER  ++Y KK
Sbjct: 129 CSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKK 164


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  135 bits (327), Expect = 4e-33
 Identities = 66/120 (55%), Positives = 80/120 (66%), Gaps = 5/120 (4%)
 Frame = +1

Query: 133 GVQIGNACWELXCLEHGI-----PALMAXXPQTRPSGVETILSTLSQRDGAGKHVXRXVF 297
           GVQIGNACWEL CLEHGI     P   +   +   S +     T     G GK V R ++
Sbjct: 13  GVQIGNACWELYCLEHGIGPDGFPTENSEVHKNN-SYLNDGFGTFFSETGQGKFVPRSIY 71

Query: 298 VDLEPTVVDEVRTGTYRQLFHXEQXXTGKEXAGNKYARGHYTIGKEIVDLVLDRIRKLAD 477
           VDLEP V+D+VRTG Y+ LFH EQ  TGKE A N YARGHYT+GKE++D VL+RIR++AD
Sbjct: 72  VDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMAD 131



 Score = 46.8 bits (106), Expect = 2e-06
 Identities = 20/36 (55%), Positives = 26/36 (72%)
 Frame = +2

Query: 482 CTGLQGFLISTPXGGGTGLWVHLLLMERFXVDYGKK 589
           C+GLQGFL+    GGGTG  +  LL+ER  ++YGKK
Sbjct: 133 CSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKK 168


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 78.2 bits (184), Expect = 8e-16
 Identities = 42/115 (36%), Positives = 59/115 (51%)
 Frame = +1

Query: 133 GVQIGNACWELXCLEHGIPALMAXXPQTRPSGVETILSTLSQRDGAGKHVXRXVFVDLEP 312
           G Q+G A W     EHG+ +  A             L+        GK+V R V VDLEP
Sbjct: 13  GNQVGAAFWSTIADEHGLDS--AGIYHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEP 70

Query: 313 TVVDEVRTGTYRQLFHXEQXXTGKEXAGNKYARGHYTIGKEIVDLVLDRIRKLAD 477
             +D V++G +  LF  +    G+  AGN +A+GHYT G E+ D VLD +R+ A+
Sbjct: 71  GTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAE 125



 Score = 31.1 bits (67), Expect = 0.13
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +2

Query: 482 CTGLQGFLISTPXGGGTGLWVHLLLMERFXVDY 580
           C  LQGF ++   GGGTG  +  LL+ +   +Y
Sbjct: 127 CDALQGFQLTHSLGGGTGSGMGTLLLSKIREEY 159


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 38/117 (32%), Positives = 58/117 (49%), Gaps = 2/117 (1%)
 Frame = +1

Query: 133 GVQIGNACWELXCLEHGIPALMAXXPQTRPSGVETILSTLSQRDGAGKHVXRXVFVDLEP 312
           G QIG+  W+  CLEHGI             GV+       Q D   +++ R + +DLEP
Sbjct: 14  GNQIGSQFWQQLCLEHGI-GPDGTLESFATEGVDRKDVFFYQSDDT-RYIPRAILIDLEP 71

Query: 313 TVVDEVRTGTYRQLFHXEQXXTGKE--XAGNKYARGHYTIGKEIVDLVLDRIRKLAD 477
            VV+ + + TY  L++ E     K    AGN +A G Y+  + I + ++D I + AD
Sbjct: 72  RVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127



 Score = 25.4 bits (53), Expect = 6.2
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = +2

Query: 491 LQGFLISTPXGGGTGLWVHLLLMERFXVDYGKK 589
           L+GF +     GGTG  +   L+ER    Y KK
Sbjct: 132 LEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKK 164


>SPBC649.03 |rhp14||XP-A family homolog Rhp14|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 289

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = -3

Query: 165 KLPAGITDLDSXLGPTCTEMHSRILDLEFFSMLKCRM 55
           KL     +LD    P C E  S  LD ++F +  CR+
Sbjct: 100 KLRLAPLNLDPETAPKCFECDSIELDTKYFDIFHCRV 136


>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 745

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 12/28 (42%), Positives = 17/28 (60%), Gaps = 1/28 (3%)
 Frame = -3

Query: 564 RSINNK*TQSPVP-PPXGVEIRNPCRPV 484
           +++  + T  P+P PP  VE R P RPV
Sbjct: 712 QNVEQRATHKPLPRPPVQVETREPVRPV 739


>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1647

 Score = 25.0 bits (52), Expect = 8.3
 Identities = 12/33 (36%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
 Frame = -3

Query: 486 VHWVSXLXDSVQNQIYNFL--SNGVVTTGIFVA 394
           V W+S   D +++ +Y FL  S G    GI  A
Sbjct: 469 VSWISDFWDDIESDMYGFLTWSMGSQIPGIITA 501


>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
           Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1108

 Score = 25.0 bits (52), Expect = 8.3
 Identities = 10/39 (25%), Positives = 20/39 (51%)
 Frame = +2

Query: 38  TTDYNSILHLSIEKNSKSKMRECISVHVGPSXESRSVMP 154
           T ++N+ + +        ++  C ++HV  S E RS+ P
Sbjct: 692 TGEWNAFIFVKYFDRKSQEISGCGTLHVNKSDEIRSICP 730


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,303,066
Number of Sequences: 5004
Number of extensions: 41794
Number of successful extensions: 100
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 256184654
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)