Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120878.seq
(641 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g14370.1 68417.m02214 disease resistance protein (TIR-NBS-LRR... 32 0.28
At4g13610.1 68417.m02118 DNA (cytosine-5-)-methyltransferase, pu... 31 0.86
At4g39370.2 68417.m05572 ubiquitin-specific protease 27, putativ... 30 1.5
At4g39370.1 68417.m05573 ubiquitin-specific protease 27, putativ... 30 1.5
At3g56570.1 68416.m06290 SET domain-containing protein low simil... 29 2.0
At2g32910.1 68415.m04035 expressed protein 29 2.6
At1g13460.2 68414.m01575 serine/threonine protein phosphatase 2A... 29 2.6
At1g13460.1 68414.m01574 serine/threonine protein phosphatase 2A... 29 2.6
At3g12340.1 68416.m01538 immunophilin, putative / FKBP-type pept... 28 6.1
At1g64630.1 68414.m07327 protein kinase family protein contains ... 28 6.1
At2g34555.1 68415.m04244 gibberellin 2-oxidase / GA2-oxidase (GA... 27 8.0
At2g30830.1 68415.m03759 2-oxoglutarate-dependent dioxygenase, p... 27 8.0
>At4g14370.1 68417.m02214 disease resistance protein (TIR-NBS-LRR
class), putative similar to zinc finger protein
(GI:15811367) [Arabidopsis thaliana]; similar to
TIR-NBS-LRR (GI:27466164) [Arabidopsis thaliana]; similar
to disease resistance protein RPP1-WsB (GI:3860165)
[Arabidopsis thaliana]
Length = 1996
Score = 32.3 bits (70), Expect = 0.28
Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
Frame = +3
Query: 336 VFNLNSMMQAEQLIFHLIYNN-EEAVNVICDNLKYTEVFTSGTQTRYTQRLRNYKKHSST 512
VF + + L F LIY+N + ++++IC + TEV+ +G ++ Q K
Sbjct: 1033 VFRRYLRPEKDYLSFSLIYHNGDRSLDLICKDKAETEVWFAGLKSLIRQNRNKQAKSEIP 1092
Query: 513 TNPNTFCSRVSRDELRFFDVTNARTHRG 596
++ C R D T RG
Sbjct: 1093 EIHDSDCFSTGRPSTASIDFAPNNTRRG 1120
>At4g13610.1 68417.m02118 DNA (cytosine-5-)-methyltransferase,
putative similar to cytosine-5 methyltransferase (METII)
[Arabidopsis thaliana] GI:6523846; contains Pfam
profiles PF01426: BAH domain, PF00145: C-5
cytosine-specific DNA methylase
Length = 1404
Score = 30.7 bits (66), Expect = 0.86
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = +3
Query: 423 DNLKYTEVFTSGTQTRYTQRLRNYKKHSSTTNPNTFCSRVSRDEL 557
++LKY ++ + R RN K+ S+T+ PN F +++ DE+
Sbjct: 233 EDLKYAKLLQEEEHMKSMDRSRN-KRSSTTSAPNKFYIKINEDEI 276
>At4g39370.2 68417.m05572 ubiquitin-specific protease 27, putative
(UBP27) similar to GI:11993494; ubiquitin specific
protease 66 - Gallus gallus,PID:g3800764
Length = 361
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +3
Query: 300 RILIPSATNYQEVFNLNSMMQAEQLIFHLIYNNEEAVNVIC 422
R ++ + T+Y + FNL S A + + HLI + +E + V+C
Sbjct: 147 RKVMVTLTDYAKNFNLTSQQDAAEALLHLISSLQEEI-VVC 186
>At4g39370.1 68417.m05573 ubiquitin-specific protease 27, putative
(UBP27) similar to GI:11993494; ubiquitin specific
protease 66 - Gallus gallus,PID:g3800764
Length = 494
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +3
Query: 300 RILIPSATNYQEVFNLNSMMQAEQLIFHLIYNNEEAVNVIC 422
R ++ + T+Y + FNL S A + + HLI + +E + V+C
Sbjct: 147 RKVMVTLTDYAKNFNLTSQQDAAEALLHLISSLQEEI-VVC 186
>At3g56570.1 68416.m06290 SET domain-containing protein low
similarity to SP|Q43088 Ribulose-1,5 bisphosphate
carboxylase/oxygenase large subunit N-
methyltransferase, chloroplast precursor (EC 2.1.1.127)
{Pisum sativum}; contains Pfam profile PF00856: SET
domain
Length = 531
Score = 29.5 bits (63), Expect = 2.0
Identities = 25/82 (30%), Positives = 41/82 (50%), Gaps = 5/82 (6%)
Frame = +3
Query: 270 LVGNKERGIKRILIPSATNYQEVFNLNSMMQAEQLI----FHLIYNNEEAVNVICDNLKY 437
++ N + G+K I++ + EVFN +M L+ F + N + VN+ D
Sbjct: 284 MLQNDQSGLKMIMVKDVSAGAEVFNTYGLMGNAALLHRYGFTELDNPYDIVNI--DLELV 341
Query: 438 TEVFTSGTQTRYTQ-RLRNYKK 500
TE TS +RYT+ RL ++K
Sbjct: 342 TEWSTSSFTSRYTRARLALWRK 363
>At2g32910.1 68415.m04035 expressed protein
Length = 691
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1
Query: 142 DAYHDDGWFICNSHLIKRFKMSKMVLPIFDEDD 240
D+Y D ++IC H ++R + MV P +DD
Sbjct: 610 DSYRRDPYYICERHALERPPRTYMVSPGRQDDD 642
>At1g13460.2 68414.m01575 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit B', putative similar to
SWISS-PROT:Q28653 serine/threonine protein phosphatase
2A, 56 kDa regulatory subunit, delta isoform (PP2A, B
subunit, B' delta isoform, PP2A, B subunit, B56 delta
isoform, PP2A, B subunit, PR61 delta isoform, PP2A, B
subunit, R5 delta isoform, PP2A, B subunit, B'-gamma)
[Oryctolagus cuniculus]; contains Pfam domain, PF01603:
Protein phosphatase 2A regulatory B subunit (B56 family)
Length = 492
Score = 29.1 bits (62), Expect = 2.6
Identities = 22/89 (24%), Positives = 34/89 (38%)
Frame = +3
Query: 252 NTIARHLVGNKERGIKRILIPSATNYQEVFNLNSMMQAEQLIFHLIYNNEEAVNVICDNL 431
N + L + +R ++P N AE+ +F ++NN+ N+I N
Sbjct: 353 NELEEVLEATQPPEFQRCMVPLFRQVARCLNSLHFQVAERALF--LWNNDHIENLIMQNR 410
Query: 432 KYTEVFTSGTQTRYTQRLRNYKKHSSTTN 518
K R TQ+ N HS T N
Sbjct: 411 KVILPIIFPALERNTQKHWNQAVHSLTLN 439
>At1g13460.1 68414.m01574 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit B', putative similar to
SWISS-PROT:Q28653 serine/threonine protein phosphatase
2A, 56 kDa regulatory subunit, delta isoform (PP2A, B
subunit, B' delta isoform, PP2A, B subunit, B56 delta
isoform, PP2A, B subunit, PR61 delta isoform, PP2A, B
subunit, R5 delta isoform, PP2A, B subunit, B'-gamma)
[Oryctolagus cuniculus]; contains Pfam domain, PF01603:
Protein phosphatase 2A regulatory B subunit (B56 family)
Length = 492
Score = 29.1 bits (62), Expect = 2.6
Identities = 22/89 (24%), Positives = 34/89 (38%)
Frame = +3
Query: 252 NTIARHLVGNKERGIKRILIPSATNYQEVFNLNSMMQAEQLIFHLIYNNEEAVNVICDNL 431
N + L + +R ++P N AE+ +F ++NN+ N+I N
Sbjct: 353 NELEEVLEATQPPEFQRCMVPLFRQVARCLNSLHFQVAERALF--LWNNDHIENLIMQNR 410
Query: 432 KYTEVFTSGTQTRYTQRLRNYKKHSSTTN 518
K R TQ+ N HS T N
Sbjct: 411 KVILPIIFPALERNTQKHWNQAVHSLTLN 439
>At3g12340.1 68416.m01538 immunophilin, putative / FKBP-type
peptidyl-prolyl cis-trans isomerase, putative contains
Pfam profile: PF00254, FKBP-type peptidyl-prolyl
cis-trans isomerases
Length = 694
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/49 (26%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +2
Query: 119 HTSHRVRPTRIMTTDGLSATATSSNVLKCQKWF-CPFSTKTTINSKYDR 262
H+S P+R+ + +T S KC+K F +T T++++ D+
Sbjct: 415 HSSESPLPSRVTRSKARKSTLESGEPAKCEKTFEAKINTHKTLDNREDK 463
>At1g64630.1 68414.m07327 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719;
contains serine/threonine protein kinases active-site
signature, PROSITE:PS00108
Length = 524
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/56 (32%), Positives = 32/56 (57%), Gaps = 2/56 (3%)
Frame = +3
Query: 327 YQEVFNLNSMMQAE--QLIFHLIYNNEEAVNVICDNLKYTEVFTSGTQTRYTQRLR 488
Y EV LNS+ +L + + ++ +++N+I TE+FTSG+ T Y ++ R
Sbjct: 63 YSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMI------TELFTSGSLTLYRKKHR 112
>At2g34555.1 68415.m04244 gibberellin 2-oxidase / GA2-oxidase
(GA2OX3) identical to ga2ox3 [GI:4678370]
Length = 335
Score = 27.5 bits (58), Expect = 8.0
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -1
Query: 470 TRLRAACENFGIF*IVAYNVN-RLFVVIYQMKD*LFGLHHTV 348
T++ ACE FG F ++ + V L + Q F LHH++
Sbjct: 39 TQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSL 80
>At2g30830.1 68415.m03759 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967)
Length = 358
Score = 27.5 bits (58), Expect = 8.0
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 473 ITRLRAACENFGIF*IVAYNVNRLFVVIYQMKD*LFGLH 357
I ++RAA E FG F ++ + + V+ MKD + G H
Sbjct: 74 IAKVRAAVEKFGFFQVINHGIP--LEVMESMKDGIRGFH 110
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,899,050
Number of Sequences: 28952
Number of extensions: 270091
Number of successful extensions: 714
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 714
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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