BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120851.seq (616 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.16c |tim17||TIM23 translocase complex subunit Tim17|Sch... 105 7e-24 SPCC16A11.09c |tim23||mitochondrial inner membrane presequence t... 46 4e-06 SPBC25H2.04c |tim22||TIM22 inner membrane protein import complex... 42 7e-05 SPBC1105.15c |htd2||3-hydroxyacyl-ACP dehydratase Htd2 |Schizosa... 29 0.71 SPAC1F3.07c |rsc58||RSC complex subunit Rsc58|Schizosaccharomyce... 28 0.93 SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosa... 26 3.8 SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 26 3.8 SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 26 5.0 SPAC3H8.04 |||chromosome segregation protein|Schizosaccharomyces... 25 8.7 SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.7 SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po... 25 8.7 >SPAC3A12.16c |tim17||TIM23 translocase complex subunit Tim17|Schizosaccharomyces pombe|chr 1|||Manual Length = 164 Score = 105 bits (251), Expect = 7e-24 Identities = 44/79 (55%), Positives = 62/79 (78%) Frame = +3 Query: 255 LFHSIKGFRNAPLGFSRKMLGSLAAVKERSPIVGGNFAVWGGMFSTIDCSLVYMRQKEDP 434 ++HSIKG+RN+P G K + ++AA K R+P++GGNF VWGG+FST DC++ +R+KEDP Sbjct: 32 IWHSIKGWRNSPPG--EKRISAIAAAKTRAPVLGGNFGVWGGLFSTFDCAVKGVRRKEDP 89 Query: 435 WNSIMSGALTGGILAARNG 491 WN+I++G TGG LA R G Sbjct: 90 WNAIIAGFFTGGALAVRGG 108 Score = 25.4 bits (53), Expect = 6.6 Identities = 8/14 (57%), Positives = 13/14 (92%) Frame = +1 Query: 175 EYTREPCPWRILDD 216 ++TR+PCP+ IL+D Sbjct: 5 DHTRDPCPYVILND 18 >SPCC16A11.09c |tim23||mitochondrial inner membrane presequence translocase complex subunit Tim23|Schizosaccharomyces pombe|chr 3|||Manual Length = 210 Score = 46.0 bits (104), Expect = 4e-06 Identities = 24/67 (35%), Positives = 37/67 (55%) Frame = +3 Query: 306 KMLGSLAAVKERSPIVGGNFAVWGGMFSTIDCSLVYMRQKEDPWNSIMSGALTGGILAAR 485 ++ G L V R P VG + V +++ I+ + Y RQK NS+ +GALTG + + Sbjct: 119 RLNGILNGVTRRGPFVGNSLGVLALVYNGINSLIGYKRQKHGWENSVAAGALTGALYKST 178 Query: 486 NGVPAMA 506 G+ AMA Sbjct: 179 RGLRAMA 185 >SPBC25H2.04c |tim22||TIM22 inner membrane protein import complex subunit Tim22|Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 41.9 bits (94), Expect = 7e-05 Identities = 21/55 (38%), Positives = 31/55 (56%) Frame = +3 Query: 339 RSPIVGGNFAVWGGMFSTIDCSLVYMRQKEDPWNSIMSGALTGGILAARNGVPAM 503 RS NF + G ++S +C + R K D +N+I +G TGG LA R+G A+ Sbjct: 99 RSFSTAKNFGLLGLIYSGSECCIEAFRAKTDIYNAIAAGVFTGGALAVRSGPKAI 153 >SPBC1105.15c |htd2||3-hydroxyacyl-ACP dehydratase Htd2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 300 Score = 28.7 bits (61), Expect = 0.71 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = -2 Query: 393 W*RTYHPKQQNYPRLLESAPLLQPNCQAFF 304 W TY K +NYP L+ PLL + +F Sbjct: 211 WNATYATKSENYPNLVLPGPLLVTSMLEYF 240 >SPAC1F3.07c |rsc58||RSC complex subunit Rsc58|Schizosaccharomyces pombe|chr 1|||Manual Length = 403 Score = 28.3 bits (60), Expect = 0.93 Identities = 16/43 (37%), Positives = 27/43 (62%) Frame = -1 Query: 409 TSEQSMVENIPPQTAKLPPTIGERSFTAAKLPSIFLLKPSGAF 281 + E ++ + PP TAK P ++ E + + AKLP++ L+ SG F Sbjct: 169 SQEYTLDKLSPPSTAKPPASVIEFNPSLAKLPTVKYLQ-SGPF 210 >SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1215 Score = 26.2 bits (55), Expect = 3.8 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = -2 Query: 396 LW*RTYHPKQQNYPRLLESAP 334 +W + PK NYP LLES+P Sbjct: 908 IWDQRLLPKIVNYPPLLESSP 928 >SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit Bgs4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1955 Score = 26.2 bits (55), Expect = 3.8 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = -2 Query: 219 RVIKDTPRAWFSCIFIHDILI 157 ++ DTPRA F+ +F D+LI Sbjct: 1609 KISMDTPRAKFTNVFFSDVLI 1629 >SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 741 Score = 25.8 bits (54), Expect = 5.0 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = -3 Query: 503 HCRYTISCSQDTPRE--GSTHNGIPWIFLLPHVH 408 HC + C ++ P E GS H P +F++PH H Sbjct: 143 HCTCPL-CRKEVPHETVGSAHP--PILFIIPHSH 173 >SPAC3H8.04 |||chromosome segregation protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 338 Score = 25.0 bits (52), Expect = 8.7 Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = +3 Query: 288 PLGFSRKMLGSLAAV-KERSPIVGGNFAVWGGMFSTIDCSLVYMRQK 425 P G+ +G L + ++ + +V F V + S +DCS ++RQK Sbjct: 224 PFGYRIPSVGQLQLILRQTNGLVIKVFLVPYNVSSMVDCSKTWIRQK 270 >SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 773 Score = 25.0 bits (52), Expect = 8.7 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = -2 Query: 441 NSMDLPFASCTQVSSLW*RTYHPKQQNYPRLLESAPL 331 N ++ P+ C QV + YH K + RL S PL Sbjct: 35 NGVESPYYICVQVENQGIEVYHMKDE---RLFASCPL 68 >SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 594 Score = 25.0 bits (52), Expect = 8.7 Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 4/35 (11%) Frame = +1 Query: 481 QEMVYRQWQECTYW----RNSLSSNRRHWYHVHKI 573 Q +VY + YW R+ +S+NR+ H+H I Sbjct: 9 QVLVYNYSKRKNYWHFWLRSFMSNNRKFLVHIHLI 43 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,690,246 Number of Sequences: 5004 Number of extensions: 59373 Number of successful extensions: 169 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 269634532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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