BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120845.seq (692 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_14872| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.6 SB_57| Best HMM Match : zf-C2H2 (HMM E-Value=0) 29 4.7 SB_45379| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.2 SB_5553| Best HMM Match : Toxin_20 (HMM E-Value=4.6) 28 6.2 SB_19025| Best HMM Match : Keratin_B2 (HMM E-Value=1.3) 28 8.3 SB_51040| Best HMM Match : Kinesin (HMM E-Value=5.89947e-43) 28 8.3 >SB_14872| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1182 Score = 29.1 bits (62), Expect = 3.6 Identities = 16/71 (22%), Positives = 33/71 (46%) Frame = +3 Query: 336 IPSATNYQEVFNLNSMMQAEQLIFHLIYNNEEAVNVICXNLKYTEVFTSGTQRLYTALRN 515 +P T + N+ + A Q F+ +Y+N+ + +C L+Y +G +RL ++ Sbjct: 844 VPDPTGKSALENILKIWCARQPEFYGVYDNKVSTLALCNVLQY--FINTGDKRLQVVVKG 901 Query: 516 YKKHSRHHKPE 548 + + PE Sbjct: 902 DRIYPNDEAPE 912 >SB_57| Best HMM Match : zf-C2H2 (HMM E-Value=0) Length = 1498 Score = 28.7 bits (61), Expect = 4.7 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Frame = +3 Query: 420 NNEEAVNVICXNLKYTEVFTSGT-QRLYTALRNYKKHSRHHKPEHVLFAC 566 N + VN C K E +T + ++ + +YK H R+H + + F C Sbjct: 895 NLDAHVNAGCPKKKPQETYTCEVCDKGFSRVSSYKTHFRYHSKDTLTFKC 944 >SB_45379| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 122 Score = 28.3 bits (60), Expect = 6.2 Identities = 13/50 (26%), Positives = 24/50 (48%) Frame = +3 Query: 471 VFTSGTQRLYTALRNYKKHSRHHKPEHVLFACVARRIAFFRRDQRPNAST 620 +F GT + + L YK+ + L+ C I FR+++R + S+ Sbjct: 15 LFLPGTSKEFFTLEKYKQAALKDYKRITLYLCTVNDIEEFRKEERDSDSS 64 >SB_5553| Best HMM Match : Toxin_20 (HMM E-Value=4.6) Length = 299 Score = 28.3 bits (60), Expect = 6.2 Identities = 13/50 (26%), Positives = 24/50 (48%) Frame = +3 Query: 471 VFTSGTQRLYTALRNYKKHSRHHKPEHVLFACVARRIAFFRRDQRPNAST 620 +F GT + + L YK+ + L+ C I FR+++R + S+ Sbjct: 103 LFLPGTSKEFFTLEKYKQAALKDYKRITLYLCTVNDIEEFRKEERDSDSS 152 >SB_19025| Best HMM Match : Keratin_B2 (HMM E-Value=1.3) Length = 227 Score = 27.9 bits (59), Expect = 8.3 Identities = 17/54 (31%), Positives = 25/54 (46%) Frame = +1 Query: 424 TKRRLTLYAXI*NIPKFSQAARNGYTQRYATTRSILDTTNPNTFCSRVSRDELR 585 T+RR+ Y + IP+ S +A Y Q A + + P F + V D LR Sbjct: 164 TRRRINRYQRLRQIPESSPSATREYRQVLARRHGTFEFSEP-VFGNEVPPDALR 216 >SB_51040| Best HMM Match : Kinesin (HMM E-Value=5.89947e-43) Length = 197 Score = 27.9 bits (59), Expect = 8.3 Identities = 17/63 (26%), Positives = 32/63 (50%), Gaps = 2/63 (3%) Frame = +3 Query: 246 AHF-DEDDNQFKMTIARHLVGNKERGIKRILIPSATNYQEVFN-LNSMMQAEQLIFHLIY 419 AHF D+ + +L GN + + + PSA NY+E + L +A++++ H + Sbjct: 131 AHFVPYRDSVLTWLLKDNLGGNSKTVMVATISPSADNYEETLSTLRYADRAKKIVNHAVV 190 Query: 420 NNE 428 N + Sbjct: 191 NED 193 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,633,222 Number of Sequences: 59808 Number of extensions: 424680 Number of successful extensions: 939 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 870 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 936 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1805522550 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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