SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120839.seq
         (692 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd...    81   7e-16
At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd...    79   4e-15
At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd...    78   6e-15
At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd...    76   3e-14
At1g21200.1 68414.m02650 expressed protein                             29   2.2  
At1g76870.1 68414.m08945 hypothetical protein                          28   6.8  
At5g15540.1 68418.m01819 expressed protein low similarity to DNA...    27   8.9  
At4g26500.1 68417.m03813 BolA-like family protein / Fe-S metabol...    27   8.9  

>At4g00620.1 68417.m00086 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 360

 Score = 81.0 bits (191), Expect = 7e-16
 Identities = 38/93 (40%), Positives = 58/93 (62%), Gaps = 2/93 (2%)
 Frame = +2

Query: 236 GHRCININRDSDITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDID 415
           G +   +    D +EE++L  +   N+D SV GILVQLP+P  ++E+ + +A++ EKD+D
Sbjct: 128 GIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSIEKDVD 187

Query: 416 GFHIINIGRLCV--DLPTIVPATALAVVEMLKR 508
           GFH +NIGRL +    P  VP T    +E+L R
Sbjct: 188 GFHPLNIGRLAMRGREPLFVPCTPKGCIELLHR 220



 Score = 44.8 bits (101), Expect = 6e-05
 Identities = 24/61 (39%), Positives = 37/61 (60%)
 Frame = +3

Query: 60  ARILDGKALAMEIKKELKQKIAQWVGLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVG 239
           A ++DGKA+A +I+ E+  ++++        P +  I+VGD   S TYVRNK +A   VG
Sbjct: 69  AIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKKKACDSVG 128

Query: 240 I 242
           I
Sbjct: 129 I 129



 Score = 30.3 bits (65), Expect = 1.3
 Identities = 21/46 (45%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
 Frame = +3

Query: 522 IRSQR-VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656
           I+ +R VVIGRS  VGMP A++L  +         DATV+I H  T
Sbjct: 225 IKGKRAVVIGRSNIVGMPAALLLQRE---------DATVSIIHSRT 261


>At4g00600.1 68417.m00084 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
           precursor (C1-THF synthase) [Includes:
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
           Formyltetrahydrofolate synthetase (EC 6.3.4.3)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           NAD(P)-binding domain, PF00763: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, catalytic domain
          Length = 310

 Score = 78.6 bits (185), Expect = 4e-15
 Identities = 36/82 (43%), Positives = 55/82 (67%), Gaps = 2/82 (2%)
 Frame = +2

Query: 269 DITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLC 448
           D +EE++L  +   N+D SV G+LVQLP+P  ++E+ + +A++ EKD+DGFH +NIGRL 
Sbjct: 89  DSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLA 148

Query: 449 V--DLPTIVPATALAVVEMLKR 508
           +    P  VP T    +E+L R
Sbjct: 149 MRGREPLFVPCTPKGCIELLHR 170



 Score = 30.3 bits (65), Expect = 1.3
 Identities = 19/40 (47%), Positives = 23/40 (57%)
 Frame = +3

Query: 537 VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656
           VVIGRS  VGMP A++L  +         DATV+I H  T
Sbjct: 181 VVIGRSNIVGMPAALLLQKE---------DATVSIIHSRT 211


>At3g12290.1 68416.m01534 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 299

 Score = 77.8 bits (183), Expect = 6e-15
 Identities = 39/100 (39%), Positives = 60/100 (60%), Gaps = 5/100 (5%)
 Frame = +2

Query: 224 RKIC---GHRCININRDSDITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAI 394
           RK C   G +  ++    +++E  L+S++  LN +  V GILVQLP+P  INE  +  AI
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 395 APEKDIDGFHIINIGRLCVD--LPTIVPATALAVVEMLKR 508
           + +KD+DGFH +NIG+L +    P  +P T    +E+L R
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLAR 160



 Score = 43.2 bits (97), Expect = 2e-04
 Identities = 22/61 (36%), Positives = 34/61 (55%)
 Frame = +3

Query: 60  ARILDGKALAMEIKKELKQKIAQWVGLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVG 239
           A+I+DGKA+A  I+ E+ +++        + P +  +IVG    S TYV  K +A   VG
Sbjct: 9   AKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKACAEVG 68

Query: 240 I 242
           I
Sbjct: 69  I 69



 Score = 31.9 bits (69), Expect = 0.41
 Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
 Frame = +3

Query: 522 IRSQR-VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656
           I+ QR VV+GRS  VG+P++++L         L  DATVT  H +T
Sbjct: 165 IKGQRAVVVGRSNIVGLPVSLLL---------LKADATVTTVHSHT 201


>At2g38660.1 68415.m04748 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
           precursor (C1-THF synthase) [Includes:
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
           Formyltetrahydrofolate synthetase (EC 6.3.4.3)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           NAD(P)-binding domain, PF00763: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, catalytic domain
          Length = 352

 Score = 75.8 bits (178), Expect = 3e-14
 Identities = 36/82 (43%), Positives = 52/82 (63%), Gaps = 2/82 (2%)
 Frame = +2

Query: 269 DITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLC 448
           D TE Q++S ++  NED S+ GILVQLP+P  +NE ++ + +  EKD+DGFH +N+G L 
Sbjct: 133 DCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLEKDVDGFHPLNVGNLA 192

Query: 449 V--DLPTIVPATALAVVEMLKR 508
           +    P  V  T    VE+L R
Sbjct: 193 MRGREPLFVSCTPKGCVELLIR 214



 Score = 44.4 bits (100), Expect = 7e-05
 Identities = 18/62 (29%), Positives = 37/62 (59%)
 Frame = +3

Query: 66  ILDGKALAMEIKKELKQKIAQWVGLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVGID 245
           ++DG  +A EI+ ++  ++ +      + P +  ++VG+   S TYVRNK++A +  GI 
Sbjct: 65  VIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGIK 124

Query: 246 AL 251
           ++
Sbjct: 125 SV 126



 Score = 29.9 bits (64), Expect = 1.7
 Identities = 18/40 (45%), Positives = 26/40 (65%)
 Frame = +3

Query: 537 VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656
           VVIGRS  VG+P++++L   +RH      DATV+  H +T
Sbjct: 225 VVIGRSNIVGLPMSLLL---QRH------DATVSTVHAFT 255


>At1g21200.1 68414.m02650 expressed protein
          Length = 443

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = -2

Query: 508 SFQHFHYSQCCSRHNGR*IHTQPSDVDYVETIDIFFRS 395
           S +H  Y + CS HNG  +H  P D+    ++ +  RS
Sbjct: 228 SSKHLFYEEMCSYHNGNRLHL-PHDLALQRSLQLALRS 264


>At1g76870.1 68414.m08945 hypothetical protein
          Length = 385

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = -2

Query: 529 DRMCRC*SFQHFHYSQCCSRHNGR*IH 449
           D + R  S +H  Y + CS HNG  +H
Sbjct: 186 DEVRRIMSSKHLFYEEMCSYHNGNRLH 212


>At5g15540.1 68418.m01819 expressed protein low similarity to DNA
            repair and meiosis protein Rad9 [Coprinus cinereus]
            GI:1353390, SP|Q09725 Sister chromatid cohesion protein
            mis4 {Schizosaccharomyces pombe}
          Length = 1755

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 24/84 (28%), Positives = 39/84 (46%)
 Frame = +2

Query: 278  EEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLCVDL 457
            E+QL SE  + N  NSV+     +PV     +  +C  I     +  F    +GR C+D 
Sbjct: 1250 EKQLGSEKASDNTVNSVEQGGHNVPVAAGAGDTNICGGI-----VQLFWDKILGR-CLDF 1303

Query: 458  PTIVPATALAVVEMLKRLTSTHSV 529
               +  T+L +VE++ R    H +
Sbjct: 1304 DDQIRQTSLKIVEVVLRQGLVHPI 1327


>At4g26500.1 68417.m03813 BolA-like family protein / Fe-S metabolism
           associated domain-containing protein similar to SufE
           protein [Erwinia chrysanthemi] GI:11342550; contains
           Pfam profiles PF02657: Fe-S metabolism associated
           domain, PF01722: BolA-like protein
          Length = 371

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = +3

Query: 9   GLSTWFENSKNNNFIIMARILDGKALAMEIKKE 107
           GL      S+NN  + M +++  KAL +E+K E
Sbjct: 189 GLQQSLSPSRNNGLLNMLKLMQKKALHLEVKGE 221


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,978,398
Number of Sequences: 28952
Number of extensions: 339477
Number of successful extensions: 845
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 821
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 845
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -