BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120839.seq (692 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 81 7e-16 At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd... 79 4e-15 At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd... 78 6e-15 At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd... 76 3e-14 At1g21200.1 68414.m02650 expressed protein 29 2.2 At1g76870.1 68414.m08945 hypothetical protein 28 6.8 At5g15540.1 68418.m01819 expressed protein low similarity to DNA... 27 8.9 At4g26500.1 68417.m03813 BolA-like family protein / Fe-S metabol... 27 8.9 >At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 360 Score = 81.0 bits (191), Expect = 7e-16 Identities = 38/93 (40%), Positives = 58/93 (62%), Gaps = 2/93 (2%) Frame = +2 Query: 236 GHRCININRDSDITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDID 415 G + + D +EE++L + N+D SV GILVQLP+P ++E+ + +A++ EKD+D Sbjct: 128 GIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSIEKDVD 187 Query: 416 GFHIINIGRLCV--DLPTIVPATALAVVEMLKR 508 GFH +NIGRL + P VP T +E+L R Sbjct: 188 GFHPLNIGRLAMRGREPLFVPCTPKGCIELLHR 220 Score = 44.8 bits (101), Expect = 6e-05 Identities = 24/61 (39%), Positives = 37/61 (60%) Frame = +3 Query: 60 ARILDGKALAMEIKKELKQKIAQWVGLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVG 239 A ++DGKA+A +I+ E+ ++++ P + I+VGD S TYVRNK +A VG Sbjct: 69 AIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKKKACDSVG 128 Query: 240 I 242 I Sbjct: 129 I 129 Score = 30.3 bits (65), Expect = 1.3 Identities = 21/46 (45%), Positives = 27/46 (58%), Gaps = 1/46 (2%) Frame = +3 Query: 522 IRSQR-VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656 I+ +R VVIGRS VGMP A++L + DATV+I H T Sbjct: 225 IKGKRAVVIGRSNIVGMPAALLLQRE---------DATVSIIHSRT 261 >At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 310 Score = 78.6 bits (185), Expect = 4e-15 Identities = 36/82 (43%), Positives = 55/82 (67%), Gaps = 2/82 (2%) Frame = +2 Query: 269 DITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLC 448 D +EE++L + N+D SV G+LVQLP+P ++E+ + +A++ EKD+DGFH +NIGRL Sbjct: 89 DSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLA 148 Query: 449 V--DLPTIVPATALAVVEMLKR 508 + P VP T +E+L R Sbjct: 149 MRGREPLFVPCTPKGCIELLHR 170 Score = 30.3 bits (65), Expect = 1.3 Identities = 19/40 (47%), Positives = 23/40 (57%) Frame = +3 Query: 537 VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656 VVIGRS VGMP A++L + DATV+I H T Sbjct: 181 VVIGRSNIVGMPAALLLQKE---------DATVSIIHSRT 211 >At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 299 Score = 77.8 bits (183), Expect = 6e-15 Identities = 39/100 (39%), Positives = 60/100 (60%), Gaps = 5/100 (5%) Frame = +2 Query: 224 RKIC---GHRCININRDSDITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAI 394 RK C G + ++ +++E L+S++ LN + V GILVQLP+P INE + AI Sbjct: 61 RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120 Query: 395 APEKDIDGFHIINIGRLCVD--LPTIVPATALAVVEMLKR 508 + +KD+DGFH +NIG+L + P +P T +E+L R Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLAR 160 Score = 43.2 bits (97), Expect = 2e-04 Identities = 22/61 (36%), Positives = 34/61 (55%) Frame = +3 Query: 60 ARILDGKALAMEIKKELKQKIAQWVGLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVG 239 A+I+DGKA+A I+ E+ +++ + P + +IVG S TYV K +A VG Sbjct: 9 AKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKACAEVG 68 Query: 240 I 242 I Sbjct: 69 I 69 Score = 31.9 bits (69), Expect = 0.41 Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 1/46 (2%) Frame = +3 Query: 522 IRSQR-VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656 I+ QR VV+GRS VG+P++++L L DATVT H +T Sbjct: 165 IKGQRAVVVGRSNIVGLPVSLLL---------LKADATVTTVHSHT 201 >At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 352 Score = 75.8 bits (178), Expect = 3e-14 Identities = 36/82 (43%), Positives = 52/82 (63%), Gaps = 2/82 (2%) Frame = +2 Query: 269 DITEEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLC 448 D TE Q++S ++ NED S+ GILVQLP+P +NE ++ + + EKD+DGFH +N+G L Sbjct: 133 DCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLEKDVDGFHPLNVGNLA 192 Query: 449 V--DLPTIVPATALAVVEMLKR 508 + P V T VE+L R Sbjct: 193 MRGREPLFVSCTPKGCVELLIR 214 Score = 44.4 bits (100), Expect = 7e-05 Identities = 18/62 (29%), Positives = 37/62 (59%) Frame = +3 Query: 66 ILDGKALAMEIKKELKQKIAQWVGLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVGID 245 ++DG +A EI+ ++ ++ + + P + ++VG+ S TYVRNK++A + GI Sbjct: 65 VIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGIK 124 Query: 246 AL 251 ++ Sbjct: 125 SV 126 Score = 29.9 bits (64), Expect = 1.7 Identities = 18/40 (45%), Positives = 26/40 (65%) Frame = +3 Query: 537 VVIGRSKNVGMPIAMMLHSDKRHESGLGMDATVTICHRYT 656 VVIGRS VG+P++++L +RH DATV+ H +T Sbjct: 225 VVIGRSNIVGLPMSLLL---QRH------DATVSTVHAFT 255 >At1g21200.1 68414.m02650 expressed protein Length = 443 Score = 29.5 bits (63), Expect = 2.2 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = -2 Query: 508 SFQHFHYSQCCSRHNGR*IHTQPSDVDYVETIDIFFRS 395 S +H Y + CS HNG +H P D+ ++ + RS Sbjct: 228 SSKHLFYEEMCSYHNGNRLHL-PHDLALQRSLQLALRS 264 >At1g76870.1 68414.m08945 hypothetical protein Length = 385 Score = 27.9 bits (59), Expect = 6.8 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = -2 Query: 529 DRMCRC*SFQHFHYSQCCSRHNGR*IH 449 D + R S +H Y + CS HNG +H Sbjct: 186 DEVRRIMSSKHLFYEEMCSYHNGNRLH 212 >At5g15540.1 68418.m01819 expressed protein low similarity to DNA repair and meiosis protein Rad9 [Coprinus cinereus] GI:1353390, SP|Q09725 Sister chromatid cohesion protein mis4 {Schizosaccharomyces pombe} Length = 1755 Score = 27.5 bits (58), Expect = 8.9 Identities = 24/84 (28%), Positives = 39/84 (46%) Frame = +2 Query: 278 EEQLLSEIQNLNEDNSVDGILVQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLCVDL 457 E+QL SE + N NSV+ +PV + +C I + F +GR C+D Sbjct: 1250 EKQLGSEKASDNTVNSVEQGGHNVPVAAGAGDTNICGGI-----VQLFWDKILGR-CLDF 1303 Query: 458 PTIVPATALAVVEMLKRLTSTHSV 529 + T+L +VE++ R H + Sbjct: 1304 DDQIRQTSLKIVEVVLRQGLVHPI 1327 >At4g26500.1 68417.m03813 BolA-like family protein / Fe-S metabolism associated domain-containing protein similar to SufE protein [Erwinia chrysanthemi] GI:11342550; contains Pfam profiles PF02657: Fe-S metabolism associated domain, PF01722: BolA-like protein Length = 371 Score = 27.5 bits (58), Expect = 8.9 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +3 Query: 9 GLSTWFENSKNNNFIIMARILDGKALAMEIKKE 107 GL S+NN + M +++ KAL +E+K E Sbjct: 189 GLQQSLSPSRNNGLLNMLKLMQKKALHLEVKGE 221 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,978,398 Number of Sequences: 28952 Number of extensions: 339477 Number of successful extensions: 845 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 821 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 845 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1477286152 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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