Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120837.seq
(669 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30260.1 68414.m03701 expressed protein 35 0.056
At3g13920.1 68416.m01758 eukaryotic translation initiation facto... 30 1.6
At4g23470.1 68417.m03382 hydroxyproline-rich glycoprotein family... 28 4.9
At5g23890.1 68418.m02806 expressed protein weak similarity to SP... 28 6.5
At2g28310.2 68415.m03438 expressed protein contains Pfam profile... 28 6.5
At2g28310.1 68415.m03437 expressed protein contains Pfam profile... 28 6.5
At1g54270.1 68414.m06187 eukaryotic translation initiation facto... 28 6.5
At4g23470.2 68417.m03383 hydroxyproline-rich glycoprotein family... 27 8.5
At1g16970.1 68414.m02061 Ku70-like protein identical to Ku70-lik... 27 8.5
At1g15500.1 68414.m01865 chloroplast ADP, ATP carrier protein, p... 27 8.5
>At1g30260.1 68414.m03701 expressed protein
Length = 97
Score = 34.7 bits (76), Expect = 0.056
Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 7/66 (10%)
Frame = +3
Query: 306 DIVKEGSNKVGTNSIFLGTVYDYGVKSP-------NAASTSSNVTMTRGTANFDIKEFKS 464
D +KE +VGT+SIF + + SP + +S S++ + T G F + E +
Sbjct: 31 DTIKEEEREVGTDSIFPSSFNSKKISSPFTSPYSSSVSSASASASCTSGLNKFPVTENRG 90
Query: 465 MFIVFK 482
F VFK
Sbjct: 91 SFPVFK 96
>At3g13920.1 68416.m01758 eukaryotic translation initiation factor
4A-1 / eIF-4A-1 eIF-4A-1 gi:15293046, gi:15450485;
contains Pfam profile PF00270: DEAD/DEAH box helicase;
contains Pfam profile PF00271: Helicase conserved
C-terminal domain
Length = 412
Score = 29.9 bits (64), Expect = 1.6
Identities = 21/70 (30%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
Frame = +3
Query: 228 GGQFAHRTRDNKLSQMYIAEKPL--SIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAAS 401
G QF R D KL+++ + S DD V E + +G L +Y YG + P+A
Sbjct: 8 GTQFDARQFDQKLNEVLEGQDEFFTSYDD-VHESFDAMGLQENLLRGIYAYGFEKPSAIQ 66
Query: 402 TSSNVTMTRG 431
V +G
Sbjct: 67 QRGIVPFCKG 76
>At4g23470.1 68417.m03382 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 255
Score = 28.3 bits (60), Expect = 4.9
Identities = 15/50 (30%), Positives = 20/50 (40%)
Frame = +3
Query: 516 ACCDSKSTA*LCA*STPTRGRCPNERCANCANPTALWCTRKNEGSSASFI 665
A D S CA P GRC +C T ++C N +S F+
Sbjct: 52 ALYDDMSRYVCCAGYMPCSGRCGEAKCPQLCLATEVFCCFANSVASTRFL 101
>At5g23890.1 68418.m02806 expressed protein weak similarity to
SP|P12957 Caldesmon (CDM) {Gallus gallus}
Length = 946
Score = 27.9 bits (59), Expect = 6.5
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +1
Query: 112 TNALNLNSLTEASPSLGQSSESVESDENKRLNVKLNNAR 228
T ++SLT S+ QSS+ + SDE K N + +N +
Sbjct: 120 TKKQEMHSLTSQQESMIQSSDEISSDEIKVANSEESNLK 158
>At2g28310.2 68415.m03438 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 374
Score = 27.9 bits (59), Expect = 6.5
Identities = 27/86 (31%), Positives = 44/86 (51%), Gaps = 8/86 (9%)
Frame = -3
Query: 529 ESQHAIVSTVLVGVIPLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRK 350
E+ I++T+L G++ I + L SL K +P +++ LDV A GD +P + RK
Sbjct: 15 ENAKVIITTIL-GIVFGTFIGITLPSLSFKINLPSRLISSLDV-AISGDKSPEDFG-SRK 71
Query: 349 M--LLVPT------LLEPSLTMSSID 296
+ VPT LL P + ++ D
Sbjct: 72 FPEIYVPTNPRGAELLPPGIVVAKTD 97
>At2g28310.1 68415.m03437 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 374
Score = 27.9 bits (59), Expect = 6.5
Identities = 27/86 (31%), Positives = 44/86 (51%), Gaps = 8/86 (9%)
Frame = -3
Query: 529 ESQHAIVSTVLVGVIPLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRK 350
E+ I++T+L G++ I + L SL K +P +++ LDV A GD +P + RK
Sbjct: 15 ENAKVIITTIL-GIVFGTFIGITLPSLSFKINLPSRLISSLDV-AISGDKSPEDFG-SRK 71
Query: 349 M--LLVPT------LLEPSLTMSSID 296
+ VPT LL P + ++ D
Sbjct: 72 FPEIYVPTNPRGAELLPPGIVVAKTD 97
>At1g54270.1 68414.m06187 eukaryotic translation initiation factor
4A-2 / eIF-4A-2 similar to eukaryotic translation
initiation factor 4A GI:19696 from [Nicotiana
plumbaginifolia]
Length = 412
Score = 27.9 bits (59), Expect = 6.5
Identities = 18/69 (26%), Positives = 29/69 (42%), Gaps = 1/69 (1%)
Frame = +3
Query: 228 GGQFAHRTRDNKLSQMYIAEKPLSID-DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAAST 404
G QF R D +L+++ + D V E + +G L +Y YG + P+A
Sbjct: 8 GTQFDTRQFDQRLNEVLDGQDEFFTSYDEVHESFDAMGLQENLLRGIYAYGFEKPSAIQQ 67
Query: 405 SSNVTMTRG 431
V +G
Sbjct: 68 RGIVPFCKG 76
>At4g23470.2 68417.m03383 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 199
Score = 27.5 bits (58), Expect = 8.5
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = +3
Query: 549 CA*STPTRGRCPNERCANCANPTALWCTRKNEGSSASFI 665
CA P GRC +C T ++C N +S F+
Sbjct: 7 CAGYMPCSGRCGEAKCPQLCLATEVFCCFANSVASTRFL 45
>At1g16970.1 68414.m02061 Ku70-like protein identical to Ku70-like
protein GI:12006424 from [Arabidopsis thaliana];
contains Pfam profiles PF03731: Ku70/Ku80 N-terminal
alpha/beta domain, PF02735: Ku70/Ku80 beta-barrel
domain, PF03730: Ku70/Ku80 C-terminal arm, and PF02037:
SAP domain; contains TIGRfam profile TIGR00578:
ATP-dependent DNA helicase ii, 70 kDa subunit
Length = 621
Score = 27.5 bits (58), Expect = 8.5
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1
Query: 121 LNLNSLTEASPSLGQSSESVESDENKRLNVK 213
LN + LTE PS+GQ E ++ KR+ K
Sbjct: 234 LNSDELTEFMPSVGQKLEDMKDQLKKRVLAK 264
>At1g15500.1 68414.m01865 chloroplast ADP, ATP carrier protein,
putative / ADP, ATP translocase, putative / adenine
nucleotide translocase, putative strong similarity to
SP|Q39002 Chloroplast ADP,ATP carrier protein 1,
chloroplast precursor (ADP/ATP translocase 1) (Adenine
nucleotide translocase 1) {Arabidopsis thaliana};
contains Pfam profile PF03219: TLC ATP/ADP transporter
Length = 618
Score = 27.5 bits (58), Expect = 8.5
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +1
Query: 559 RPQHGAAVRTRGARIAQIQLHSGVHEKTRAAQQVLLG 669
+P HG R + + L S H+K +A QQ+ LG
Sbjct: 25 QPSHGLKQRLFTTNLPALSLSSNGHKKFQAFQQIPLG 61
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,537,297
Number of Sequences: 28952
Number of extensions: 298907
Number of successful extensions: 823
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 804
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 823
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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