BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120835.seq (416 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC12B10.15c |||ribonuclease H2 complex subunit|Schizosaccharom... 29 0.38 SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 25 3.6 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 24 8.2 >SPAC12B10.15c |||ribonuclease H2 complex subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 147 Score = 28.7 bits (61), Expect = 0.38 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = +3 Query: 63 CHIWFDTPAPFFSRYHTQTQ 122 CHI +D PAP F +H + Q Sbjct: 26 CHISYDGPAPVFEYFHDKIQ 45 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.4 bits (53), Expect = 3.6 Identities = 17/76 (22%), Positives = 34/76 (44%), Gaps = 7/76 (9%) Frame = -2 Query: 406 FFLNDKVIYLQNSNKNKLFELSGL-----SLKSCRHD--FVTVESQTRAGDEIASFLRTV 248 F +V+YL ++NK + FELS + + +H F+ + T +E + + + Sbjct: 374 FLQQSRVLYLFHANKQRHFELSDILGNVVLFSTDQHGSRFIQQKLATATEEEREAVFQEI 433 Query: 247 GCVECLAVNSSVXCNF 200 CL + + N+ Sbjct: 434 ASTSCLQLMMDIFGNY 449 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 24.2 bits (50), Expect = 8.2 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = -2 Query: 364 KNKLFELSGLSLKSCRHDFVTVESQTRAGDEIASF 260 KNKL + S + L SC + S TR + +S+ Sbjct: 78 KNKLSKESAIQLSSCIRKTLLAPSSTRVPSKNSSY 112 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,469,525 Number of Sequences: 5004 Number of extensions: 25042 Number of successful extensions: 75 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 74 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 75 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 146319408 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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