BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120820.seq (686 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g03050.1 68415.m00258 mitochondrial transcription termination... 33 0.18 At2g03190.1 68415.m00272 E3 ubiquitin ligase SCF complex subunit... 31 0.95 At4g01650.1 68417.m00214 expressed protein 29 3.8 At1g69545.1 68414.m07997 leucine-rich repeat family protein cont... 28 6.7 At5g63020.1 68418.m07906 disease resistance protein (CC-NBS-LRR ... 27 8.8 >At2g03050.1 68415.m00258 mitochondrial transcription termination factor-related / mTERF-related contains Pfam profile PF02536: mTERF Length = 283 Score = 33.1 bits (72), Expect = 0.18 Identities = 19/73 (26%), Positives = 37/73 (50%) Frame = -3 Query: 609 RSQIKRTLRKSPPVLDRVYDTIRPHRXSFFYV*MDGEQKLLKRLQRHF*ILINVIRVDPK 430 R ++ + + +SP +L D + FF M G+ K LKR ++F + ++ P+ Sbjct: 186 REEVAKMVVRSPALLTYSVDNNLVPKVEFFIEEMRGDVKELKRFPQYFSFSLE-RKIKPR 244 Query: 429 NRRVVEHNVIRRL 391 +R + EH ++ L Sbjct: 245 HRLLKEHGILMPL 257 >At2g03190.1 68415.m00272 E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 (At16), putative E3 ubiquitin ligase; similar to Skp1 homolog Skp1a GI:3068807 from [Arabidopsis thaliana] Length = 170 Score = 30.7 bits (66), Expect = 0.95 Identities = 15/45 (33%), Positives = 24/45 (53%) Frame = +1 Query: 94 SILVVMYESMEKHFSNVVDEIDSLKTDTFMMLSNLQNNTIRTWDA 228 +IL ++ E +KH + VD+ D T ++ N +RTWDA Sbjct: 50 NILALVIEYCKKHVLDDVDDSDDSTEATSENVNEEAKNELRTWDA 94 >At4g01650.1 68417.m00214 expressed protein Length = 288 Score = 28.7 bits (61), Expect = 3.8 Identities = 15/32 (46%), Positives = 17/32 (53%) Frame = -2 Query: 304 LTTPFFVNSTLIFSSRLIFFCHFLQLRPTFES 209 L T F +STL+ SSR F C F P F S Sbjct: 40 LITSFSPSSTLLASSRRCFTCRFGDSSPRFNS 71 >At1g69545.1 68414.m07997 leucine-rich repeat family protein contains Pfam PF00560: Leucine Rich Repeat domains; similar to disease resistance protein RPP1-WsA (GI:3860163)[Arabidopsis thaliana] Length = 703 Score = 27.9 bits (59), Expect = 6.7 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = -1 Query: 188 NIINVSVLRESISSTTLEKCFSIDSYMTTSIDFISCSSLFKCPA 57 N+IN+ L S S+ +E SI + +D CSSL + P+ Sbjct: 377 NLINLKTLNLSGCSSLVELPSSIGNLNLKKLDLSGCSSLVELPS 420 >At5g63020.1 68418.m07906 disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease resistance protein. Length = 888 Score = 27.5 bits (58), Expect = 8.8 Identities = 16/66 (24%), Positives = 34/66 (51%) Frame = +1 Query: 55 YAGHLNNELQEIKSILVVMYESMEKHFSNVVDEIDSLKTDTFMMLSNLQNNTIRTWDAVV 234 + GH+++ + +LVV+ SM + ++ +EI + ++ LS + IR W A + Sbjct: 544 FLGHISSSFFRLMPMLVVLDLSMNRDLRHLPNEISECVSLQYLSLSRTR---IRIWPAGL 600 Query: 235 KNGKKI 252 +K+ Sbjct: 601 VELRKL 606 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,652,466 Number of Sequences: 28952 Number of extensions: 261297 Number of successful extensions: 672 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 661 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 672 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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