Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120819.seq
         (686 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo...    31   0.16 
SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo...    30   0.36 
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    27   2.5  
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9...    27   3.4  
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz...    26   4.4  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    25   7.8  
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom...    25   7.8  
SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyce...    25   7.8  

>SPBC19G7.10c |||topoisomerase associated protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 744

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
 Frame = -3

Query: 456 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 277
           P+  +   L  L+S  A  R   T L +  A G +   +   PR++L V    EP+ + S
Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442

Query: 276 SID--SGFS 256
           S++  SGFS
Sbjct: 443 SLNNFSGFS 451


>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
           Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 385

 Score = 29.9 bits (64), Expect = 0.36
 Identities = 28/96 (29%), Positives = 44/96 (45%), Gaps = 3/96 (3%)
 Frame = -2

Query: 415 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHFVGALFDYVVYR*RFFGIYIW 242
           K+CG  A  HGH  R   R+W F   + Y  +K  ++  F  A  + V Y     GI   
Sbjct: 39  KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVFNEA-HENVKY---LPGIECP 94

Query: 241 LNLLSPCAMRKLATRA-LLSFTFNRLFSSDSTDSLL 137
            N+++   +R++A RA +L F     F     D ++
Sbjct: 95  PNVIAVPDVREVARRADILVFVVPHQFIERVCDQMV 130


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 16/48 (33%), Positives = 23/48 (47%)
 Frame = -3

Query: 354 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSEYTFGSIYYRRVRCA 211
           LTP    +P+ ++L+P L+   L  S+I SG           R VR A
Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSGSIHSDIRVSQLREVRAA 720


>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
            Srb9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1223

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = +2

Query: 275  DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 400
            D I + G NK    S+ + T+ +    SPN+ +T+SN   T+
Sbjct: 891  DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932


>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 322

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 14/52 (26%), Positives = 26/52 (50%)
 Frame = +2

Query: 278 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 433
           D +KE + K   +S+F    ++Y    PNA+    N T      ++D++E +
Sbjct: 64  DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = -3

Query: 402 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 301
           P V++    V  AF  +TP  YTVP+  +++PTL
Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415


>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1562

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 14/50 (28%), Positives = 27/50 (54%)
 Frame = +2

Query: 245 NVYSEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTM 394
           ++  E PLS +D++  GSN   T+ +F   ++  G +S    +   NV++
Sbjct: 515 SIQGEIPLSNNDVLYRGSNLRNTSELFALVIFT-GEESKIRMNAVRNVSV 563


>SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 210

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
 Frame = +3

Query: 105 SLTEASPSLGQSSESVESDENKRLNVKLN--NARVANLRIAHGDNKLSQ 245
           SLT+A  SL + S+SVE  +  +L+ +L+    ++AN R   G   L Q
Sbjct: 16  SLTDAIDSLDKRSDSVEV-KIAKLDAQLSVFQQKIANTRPGPGQTALKQ 63


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,753,921
Number of Sequences: 5004
Number of extensions: 56420
Number of successful extensions: 147
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)