Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120819.seq
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30260.1 68414.m03701 expressed protein 35 0.044
At3g03140.1 68416.m00310 expressed protein 29 0.41
At2g28310.2 68415.m03438 expressed protein contains Pfam profile... 29 2.9
At2g28310.1 68415.m03437 expressed protein contains Pfam profile... 29 2.9
At1g55810.3 68414.m06396 uracil phosphoribosyltransferase, putat... 29 2.9
At1g55810.2 68414.m06395 uracil phosphoribosyltransferase, putat... 29 2.9
At1g55810.1 68414.m06394 uracil phosphoribosyltransferase, putat... 29 2.9
At5g23890.1 68418.m02806 expressed protein weak similarity to SP... 29 3.8
At1g09960.1 68414.m01122 sucrose transporter / sucrose-proton sy... 28 6.7
At4g26540.1 68417.m03823 protein kinase family protein Three fal... 27 8.8
At1g16970.1 68414.m02061 Ku70-like protein identical to Ku70-lik... 27 8.8
>At1g30260.1 68414.m03701 expressed protein
Length = 97
Score = 35.1 bits (77), Expect = 0.044
Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 7/76 (9%)
Frame = +2
Query: 248 VYSEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSP-------NAASTSSNVTMTRGT 406
V + + D +KE +VGT+SIF + + SP + +S S++ + T G
Sbjct: 21 VMRRRTAKVLDTIKEEEREVGTDSIFPSSFNSKKISSPFTSPYSSSVSSASASASCTSGL 80
Query: 407 ANFDIKEFKSMFIVFK 454
F + E + F VFK
Sbjct: 81 NKFPVTENRGSFPVFK 96
>At3g03140.1 68416.m00310 expressed protein
Length = 769
Score = 29.1 bits (62), Expect(2) = 0.41
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 282 MSSIDSGFSEYTFGSIYYRRVRCANWPPGR 193
M+S SG ++T GSI + R R +W PGR
Sbjct: 1 MASPGSGAVDWTVGSIVWVRRRNGSWWPGR 30
Score = 21.4 bits (43), Expect(2) = 0.41
Identities = 10/40 (25%), Positives = 18/40 (45%)
Frame = -3
Query: 165 SRLIPPIRCSALNWATPRLRNLNLMHSSNRDKSVRAKRSI 46
S+ + P RC + R+ + M R+K R + +I
Sbjct: 69 SKRVKPFRCGDFDECIERVESSQAMIIKKREKYARREDAI 108
>At2g28310.2 68415.m03438 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 374
Score = 29.1 bits (62), Expect = 2.9
Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 8/87 (9%)
Frame = -3
Query: 504 SNRNMPLSSTVLVGVIPLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPR 325
+N N + T ++G++ I + L SL K +P +++ LDV A GD +P + R
Sbjct: 13 TNENAKVIITTILGIVFGTFIGITLPSLSFKINLPSRLISSLDV-AISGDKSPEDFG-SR 70
Query: 324 KM--LLVPT------LLEPSLTMSSID 268
K + VPT LL P + ++ D
Sbjct: 71 KFPEIYVPTNPRGAELLPPGIVVAKTD 97
>At2g28310.1 68415.m03437 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 374
Score = 29.1 bits (62), Expect = 2.9
Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 8/87 (9%)
Frame = -3
Query: 504 SNRNMPLSSTVLVGVIPLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPR 325
+N N + T ++G++ I + L SL K +P +++ LDV A GD +P + R
Sbjct: 13 TNENAKVIITTILGIVFGTFIGITLPSLSFKINLPSRLISSLDV-AISGDKSPEDFG-SR 70
Query: 324 KM--LLVPT------LLEPSLTMSSID 268
K + VPT LL P + ++ D
Sbjct: 71 KFPEIYVPTNPRGAELLPPGIVVAKTD 97
>At1g55810.3 68414.m06396 uracil phosphoribosyltransferase, putative
/ UMP pyrophosphorylase, putative / UPRTase, putative
similar to SP|O65583 Uracil phosphoribosyltransferase
(EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase)
{Arabidopsis thaliana}; contains Pfam profile PF00485:
Phosphoribulokinase / Uridine kinase family
Length = 466
Score = 29.1 bits (62), Expect = 2.9
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 8/82 (9%)
Frame = +2
Query: 281 IVKEGSNKVGTNSIFLGTVYDYGVKSPNAAST----SSNVTMTRGTA----NFDIKEFKS 436
+V + S N + L V+DY P+A T SS + +G A N+D K +K+
Sbjct: 75 VVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNYDFKSYKN 134
Query: 437 MFIVFKGITPTKTVEDNGMLRF 502
+ + P+ + G+L F
Sbjct: 135 NVFPPRRVNPSDVIILEGILIF 156
>At1g55810.2 68414.m06395 uracil phosphoribosyltransferase, putative
/ UMP pyrophosphorylase, putative / UPRTase, putative
similar to SP|O65583 Uracil phosphoribosyltransferase
(EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase)
{Arabidopsis thaliana}; contains Pfam profile PF00485:
Phosphoribulokinase / Uridine kinase family
Length = 466
Score = 29.1 bits (62), Expect = 2.9
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 8/82 (9%)
Frame = +2
Query: 281 IVKEGSNKVGTNSIFLGTVYDYGVKSPNAAST----SSNVTMTRGTA----NFDIKEFKS 436
+V + S N + L V+DY P+A T SS + +G A N+D K +K+
Sbjct: 75 VVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNYDFKSYKN 134
Query: 437 MFIVFKGITPTKTVEDNGMLRF 502
+ + P+ + G+L F
Sbjct: 135 NVFPPRRVNPSDVIILEGILIF 156
>At1g55810.1 68414.m06394 uracil phosphoribosyltransferase, putative
/ UMP pyrophosphorylase, putative / UPRTase, putative
similar to SP|O65583 Uracil phosphoribosyltransferase
(EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase)
{Arabidopsis thaliana}; contains Pfam profile PF00485:
Phosphoribulokinase / Uridine kinase family
Length = 466
Score = 29.1 bits (62), Expect = 2.9
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 8/82 (9%)
Frame = +2
Query: 281 IVKEGSNKVGTNSIFLGTVYDYGVKSPNAAST----SSNVTMTRGTA----NFDIKEFKS 436
+V + S N + L V+DY P+A T SS + +G A N+D K +K+
Sbjct: 75 VVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNYDFKSYKN 134
Query: 437 MFIVFKGITPTKTVEDNGMLRF 502
+ + P+ + G+L F
Sbjct: 135 NVFPPRRVNPSDVIILEGILIF 156
>At5g23890.1 68418.m02806 expressed protein weak similarity to
SP|P12957 Caldesmon (CDM) {Gallus gallus}
Length = 946
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = +3
Query: 84 TNALNLNSLTEASPSLGQSSESVESDENKRLNVKLNNARVANLRIAHGD 230
T ++SLT S+ QSS+ + SDE K N + +N + + I D
Sbjct: 120 TKKQEMHSLTSQQESMIQSSDEISSDEIKVANSEESNLKDEDKSIESND 168
>At1g09960.1 68414.m01122 sucrose transporter / sucrose-proton
symporter (SUT4) nearly identical to sucrose transporter
SUT4 [Arabidopsis thaliana] GI:9957053
Length = 510
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = -1
Query: 572 SRTSRSXSGPVLGSIKHTIM--L*LRIATCHCPLQF*WALYL 453
+R S S S PV+ + + + LR+A+ C +QF WAL L
Sbjct: 20 ARPSTSSSRPVVSPPRSKVSKRVLLRVASVACGIQFGWALQL 61
>At4g26540.1 68417.m03823 protein kinase family protein Three false
introns were added with non-consensus splice sites to
circumenvent frameshifts likely due to sequencing
errors; this is extremely unusual and is under
investigation.
Length = 1089
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +2
Query: 239 EPNVYSEKPLSIDDIVKE--GSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR-GTA 409
E +Y + SIDDIVK +N +GT S G VY + S + + + G
Sbjct: 740 EVTLYQKLDFSIDDIVKNLTSANVIGTGS--SGVVYRITIPSGESLAVKKMWSKEESGAF 797
Query: 410 NFDIKEFKSM 439
N +IK S+
Sbjct: 798 NSEIKTLGSI 807
>At1g16970.1 68414.m02061 Ku70-like protein identical to Ku70-like
protein GI:12006424 from [Arabidopsis thaliana];
contains Pfam profiles PF03731: Ku70/Ku80 N-terminal
alpha/beta domain, PF02735: Ku70/Ku80 beta-barrel
domain, PF03730: Ku70/Ku80 C-terminal arm, and PF02037:
SAP domain; contains TIGRfam profile TIGR00578:
ATP-dependent DNA helicase ii, 70 kDa subunit
Length = 621
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +3
Query: 93 LNLNSLTEASPSLGQSSESVESDENKRLNVK 185
LN + LTE PS+GQ E ++ KR+ K
Sbjct: 234 LNSDELTEFMPSVGQKLEDMKDQLKKRVLAK 264
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,236,895
Number of Sequences: 28952
Number of extensions: 290813
Number of successful extensions: 812
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 786
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 812
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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