SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120818.seq
         (669 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces ...    33   0.037
SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc...    28   1.1  
SPBC29A10.01 |ccr1|SPBC365.17|NADPH-cytochrome p450 reductase |S...    26   5.6  
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce...    26   5.6  
SPAC3F10.02c |trk1|sptrk|potassium ion transporter Trk1|Schizosa...    25   7.5  
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb...    25   7.5  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    25   9.9  
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb...    25   9.9  

>SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 296

 Score = 33.1 bits (72), Expect = 0.037
 Identities = 21/74 (28%), Positives = 31/74 (41%)
 Frame = +2

Query: 53  LNDNVNYATNKLFSKDQANGELQKLSAMLLNYKKSNKNVPNIKFDLKNLSFMLENTDKID 232
           + +N NY+      K   +GE    S    N    + N+P I  DL ++   LEN D   
Sbjct: 135 IENNPNYSNKPSKLKRALSGENSVFSIHQRNTLSPSSNLPRIPSDLYDIKQFLENLDARS 194

Query: 233 IIQFDDVKTMYNLR 274
                D+K   N+R
Sbjct: 195 FKSDMDLKRSQNMR 208


>SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 682

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 16/39 (41%), Positives = 22/39 (56%)
 Frame = +1

Query: 379 DIDNIKLSHMQLARLLCYTAVVESRNSKPWKAIFNNDTC 495
           + D +KL   QL RL  Y AVVE  + +  K I+  +TC
Sbjct: 280 EFDMVKLRQYQLERLRYYYAVVECDSVRTAKVIY--ETC 316


>SPBC29A10.01 |ccr1|SPBC365.17|NADPH-cytochrome p450 reductase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 678

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +2

Query: 182 FDLKNLSFMLENTDKIDIIQFDDVKTM 262
           F+L N+ F LEN D  D+  FD  K +
Sbjct: 79  FNLTNMVFDLENYDLTDLDNFDRSKLL 105



 Score = 25.4 bits (53), Expect = 7.5
 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
 Frame = +2

Query: 152 KSNKNVPNIKFDLKNLSFM-LENTDKIDIIQF 244
           K+N N+ N+ FDL+N     L+N D+  ++ F
Sbjct: 76  KANFNLTNMVFDLENYDLTDLDNFDRSKLLVF 107


>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1242

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 16/42 (38%), Positives = 19/42 (45%)
 Frame = -2

Query: 200  TNFLGRI*YLARFCWTFCNLTTSPTVFVTRR*LDLC*TVYWW 75
            +NF  R+  L  FCW    L T   VFV    L L   +Y W
Sbjct: 955  SNFYKRLIALLLFCWICSTLVTVLLVFVP---LSLGRAIYAW 993


>SPAC3F10.02c |trk1|sptrk|potassium ion transporter
           Trk1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 841

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 6/15 (40%), Positives = 14/15 (93%)
 Frame = -3

Query: 187 VEFNIWHVFVGLFVI 143
           + +++W++F+GLF+I
Sbjct: 715 LSYDLWYIFLGLFII 729


>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 175

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = +2

Query: 95  KDQANGELQKLSAMLLNYKK-SNKNVPNIKFDLKNLSFMLENTDK 226
           K   N +L+ ++A      + + KNV  +K +L+NLS  L+N ++
Sbjct: 84  KIDLNSQLKAINAFEAKASEGAKKNVELVKAELQNLSATLKNIEQ 128


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 12/41 (29%), Positives = 18/41 (43%)
 Frame = +2

Query: 83  KLFSKDQANGELQKLSAMLLNYKKSNKNVPNIKFDLKNLSF 205
           K   KD+ N  + K+    +NY    KN+     +LK   F
Sbjct: 182 KCILKDKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEF 222


>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 605

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 13/52 (25%), Positives = 22/52 (42%), Gaps = 3/52 (5%)
 Frame = +2

Query: 332 TRYWKTAMK---IWCQTSPTLITLNCRTCNWRACYVTRPSLSHATVNLGKQY 478
           +++WK       IW +     I   C  C W    + R +L  A  ++ K+Y
Sbjct: 137 SKHWKELADDDVIWHRMCEQHINRKCEKCGWGLPLLERNTLYAAKASIQKRY 188


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,672,276
Number of Sequences: 5004
Number of extensions: 52585
Number of successful extensions: 144
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -