Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120803.seq
         (660 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo...    31   0.15 
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    29   0.59 
SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo...    29   0.59 
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9...    27   3.2  
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz...    26   4.2  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    25   7.3  
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos...    25   9.7  

>SPBC19G7.10c |||topoisomerase associated protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 744

 Score = 31.1 bits (67), Expect = 0.15
 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
 Frame = -2

Query: 476 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 297
           P+  +   L  L+S  A  R   T L +  A G +   +   PR++L V    EP+ + S
Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442

Query: 296 SID--SGFS 276
           S++  SGFS
Sbjct: 443 SLNNFSGFS 451


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 29.1 bits (62), Expect = 0.59
 Identities = 17/48 (35%), Positives = 26/48 (54%)
 Frame = -2

Query: 374 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSAIYIWLNYYRRVRCA 231
           LTP    +P+ ++L+P L+   L  S+I SG     I ++  R VR A
Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSGSIHSDIRVSQLREVRAA 720


>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
           Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 385

 Score = 29.1 bits (62), Expect = 0.59
 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
 Frame = -1

Query: 435 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 322
           K+CG  A  HGH  R   R+W F   + Y  +K  ++  F
Sbjct: 39  KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78


>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
            Srb9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1223

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = +1

Query: 295  DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 420
            D I + G NK    S+ + T+ +    SPN+ +T+SN   T+
Sbjct: 891  DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932


>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 322

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 14/52 (26%), Positives = 26/52 (50%)
 Frame = +1

Query: 298 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 453
           D +KE + K   +S+F    ++Y    PNA+    N T      ++D++E +
Sbjct: 64  DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = -2

Query: 422 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 321
           P V++    V  AF  +TP  YTVP+  +++PTL
Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415


>SPCC1322.12c |bub1||serine/threonine protein kinase
           Bub1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1044

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 12/43 (27%), Positives = 20/43 (46%)
 Frame = +1

Query: 271 IAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTS 399
           I  +PL  + + K   + +   S +LGT       S N+ +TS
Sbjct: 396 IFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENSSNSGATS 438


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,765,443
Number of Sequences: 5004
Number of extensions: 56564
Number of successful extensions: 166
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)