Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120777.seq
(649 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 156 2e-39
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 156 3e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 156 3e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 88 9e-19
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 85 8e-18
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 64 1e-11
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 0.001
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.015
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 33 0.027
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.11
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 31 0.14
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.25
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.44
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 28 1.3
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 28 1.3
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 2.3
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 3.1
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.1
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 26 4.1
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 26 5.4
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 7.1
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 25 7.1
SPCC830.04c |mug128||sequence orphan|Schizosaccharomyces pombe|c... 25 9.4
SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces ... 25 9.4
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 9.4
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 156 bits (379), Expect = 2e-39
Identities = 73/84 (86%), Positives = 78/84 (92%)
Frame = +3
Query: 3 LKAERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 182
LKAER+ ITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCAVLI+ GTGE
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGE 122
Query: 183 FEAGISKNGQTREHALLAFTLGVK 254
FEAGISK+GQTREHALLA+TLGVK
Sbjct: 123 FEAGISKDGQTREHALLAYTLGVK 146
Score = 111 bits (266), Expect = 1e-25
Identities = 57/122 (46%), Positives = 77/122 (63%)
Frame = +2
Query: 251 QKLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPS 430
++LIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+
Sbjct: 146 KQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPT 203
Query: 431 TKMPWFKGWQVERKEGKAERKMPH*SLDAILATCPAPLTSPWRLSPGKTYYKNRVVLVTV 610
T MPW++GWQ E K G + K ++D+I P P RL Y + V V
Sbjct: 204 TNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPA-RPTDKPLRLPLQDVYKIGGIGTVPV 262
Query: 611 AR 616
R
Sbjct: 263 GR 264
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 156 bits (378), Expect = 3e-39
Identities = 72/84 (85%), Positives = 78/84 (92%)
Frame = +3
Query: 3 LKAERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 182
LKAER+ ITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGE
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGE 122
Query: 183 FEAGISKNGQTREHALLAFTLGVK 254
FEAGISK+GQTREHALLA+TLGVK
Sbjct: 123 FEAGISKDGQTREHALLAYTLGVK 146
Score = 111 bits (266), Expect = 1e-25
Identities = 57/122 (46%), Positives = 77/122 (63%)
Frame = +2
Query: 251 QKLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPS 430
++LIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+
Sbjct: 146 KQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPT 203
Query: 431 TKMPWFKGWQVERKEGKAERKMPH*SLDAILATCPAPLTSPWRLSPGKTYYKNRVVLVTV 610
T MPW++GWQ E K G + K ++D+I P P RL Y + V V
Sbjct: 204 TNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPA-RPTDKPLRLPLQDVYKIGGIGTVPV 262
Query: 611 AR 616
R
Sbjct: 263 GR 264
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 156 bits (378), Expect = 3e-39
Identities = 72/84 (85%), Positives = 78/84 (92%)
Frame = +3
Query: 3 LKAERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 182
LKAER+ ITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGE
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGE 122
Query: 183 FEAGISKNGQTREHALLAFTLGVK 254
FEAGISK+GQTREHALLA+TLGVK
Sbjct: 123 FEAGISKDGQTREHALLAYTLGVK 146
Score = 111 bits (266), Expect = 1e-25
Identities = 57/122 (46%), Positives = 77/122 (63%)
Frame = +2
Query: 251 QKLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPS 430
++LIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+
Sbjct: 146 KQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPT 203
Query: 431 TKMPWFKGWQVERKEGKAERKMPH*SLDAILATCPAPLTSPWRLSPGKTYYKNRVVLVTV 610
T MPW++GWQ E K G + K ++D+I P P RL Y + V V
Sbjct: 204 TNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPA-RPTDKPLRLPLQDVYKIGGIGTVPV 262
Query: 611 AR 616
R
Sbjct: 263 GR 264
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 88.2 bits (209), Expect = 9e-19
Identities = 39/80 (48%), Positives = 55/80 (68%)
Frame = +3
Query: 12 ERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 191
ER+ T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++A GEFEA
Sbjct: 297 EREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEA 356
Query: 192 GISKNGQTREHALLAFTLGV 251
G + GQTREHA+LA T G+
Sbjct: 357 GFERGGQTREHAVLARTQGI 376
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 85.0 bits (201), Expect = 8e-18
Identities = 42/80 (52%), Positives = 50/80 (62%)
Frame = +3
Query: 12 ERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 191
ER +T+D+A FE+ K I DAPGHRDFI MI G S AD AVL+V + FE
Sbjct: 236 ERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFER 295
Query: 192 GISKNGQTREHALLAFTLGV 251
G +NGQTREHA L LG+
Sbjct: 296 GFLENGQTREHAYLLRALGI 315
Score = 52.0 bits (119), Expect = 7e-08
Identities = 29/70 (41%), Positives = 43/70 (61%), Gaps = 3/70 (4%)
Frame = +2
Query: 254 KLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--E 424
+++V VNK+D +SE RF+EIK VS + IK +G+ + V FVPIS G N++ +
Sbjct: 317 EIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKD 374
Query: 425 PSTKMPWFKG 454
S W+KG
Sbjct: 375 SSDLYKWYKG 384
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 64.5 bits (150), Expect = 1e-11
Identities = 35/81 (43%), Positives = 49/81 (60%)
Frame = +3
Query: 12 ERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 191
E+ ITI A ++ET+ + +D PGH D+IKNMITG + D A+++V+A G+
Sbjct: 97 EKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP- 155
Query: 192 GISKNGQTREHALLAFTLGVK 254
QTREH LLA +GVK
Sbjct: 156 ------QTREHLLLARQVGVK 170
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 37.9 bits (84), Expect = 0.001
Identities = 21/59 (35%), Positives = 27/59 (45%)
Frame = +3
Query: 3 LKAERDXRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 179
L AER ITI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 71 LPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.015
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +3
Query: 54 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 224
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.5 bits (73), Expect = 0.027
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +3
Query: 54 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 179
F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 215 FDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 31.5 bits (68), Expect = 0.11
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 4/63 (6%)
Frame = +3
Query: 3 LKAERDXRITIDIA----LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 170
L+ ER IT+ ++ + Y + +ID PGH DF ++ + + +L+V A
Sbjct: 98 LEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDA 157
Query: 171 GTG 179
G
Sbjct: 158 SQG 160
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 31.1 bits (67), Expect = 0.14
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 72 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 167
+V+ +D PGH + M+ G + D A+L++A
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIA 140
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.25
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 69 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 179
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.44
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 81 IIDAPGHRDFIKNMITGTSQADCAVLIV 164
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 69 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 164
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 69 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 164
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.1 bits (57), Expect = 2.3
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +3
Query: 300 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 470
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 471 RKA 479
++
Sbjct: 205 SQS 207
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 84 IDAPGHRDFIKNMITGTSQADCAVLIV 164
ID PGH DF+ + + +D VL+V
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1073
Score = 26.2 bits (55), Expect = 4.1
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 154 CSS*LPVPVNSKLVSLRTVKPVSMPCSLSPSVSKAHRRSKQNGFH 288
C+S +PV S +VS+ T K SL SV+ S+ N +
Sbjct: 913 CNSDCELPVRSPIVSMSTYKDYVYTSSLRDSVAVFQYDSENNSLN 957
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +3
Query: 27 ITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 119
+ I+I + E +++ T+ID PG DFI N
Sbjct: 71 VEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC13G7.04c |mac1||membrane anchored protein Mac1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 756
Score = 25.8 bits (54), Expect = 5.4
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = -1
Query: 619 RPGHGNQYHPIFVIRLARGKTPGACQWGRAGGQDGIEASMR 497
RP +GN P F + +R + G+ AG G + MR
Sbjct: 671 RPSYGNMSRPSFELNGSRNPSHGSLNTAHAGMGYGPRSMMR 711
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 7.1
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +2
Query: 278 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 448
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 25.4 bits (53), Expect = 7.1
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 3/37 (8%)
Frame = +1
Query: 175 PVNSKLVSLRTVKPVSMPCSL---SPSVSKAHRRSKQ 276
P ++ SL T KPVSMP S PSV+ + S Q
Sbjct: 392 PPPPRVKSLATNKPVSMPVSTEQSDPSVAASSSSSSQ 428
>SPCC830.04c |mug128||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 143
Score = 25.0 bits (52), Expect = 9.4
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +2
Query: 443 WFKGWQVERKEGKAERKMPH*SLDAILATC 532
WF G QV R+ +++ PH + +L C
Sbjct: 77 WFLGNQVIRENFSIKKRKPHNHIPQLLEQC 106
>SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 324
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/29 (44%), Positives = 15/29 (51%)
Frame = +2
Query: 473 EGKAERKMPH*SLDAILATCPAPLTSPWR 559
+GKA R S+D TC PL PWR
Sbjct: 250 DGKAWRVQAV-SIDPTSFTCRLPLPEPWR 277
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 25.0 bits (52), Expect = 9.4
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +2
Query: 329 KEVS---SYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 454
KEVS SYI +P +G+ +N+++P + P FKG
Sbjct: 159 KEVSTSKSYISSGYLHPKTSPIFKKNGYATENVVDPISSSPRFKG 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,954,513
Number of Sequences: 5004
Number of extensions: 66148
Number of successful extensions: 212
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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