BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120742.seq (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces p... 75 1e-14 SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 31 0.21 SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 27 3.4 SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 27 3.4 SPAC3H1.02c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|... 26 4.5 SPBC1778.09 |||GTPase activating protein |Schizosaccharomyces po... 25 7.9 >SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 612 Score = 74.5 bits (175), Expect = 1e-14 Identities = 32/52 (61%), Positives = 40/52 (76%) Frame = +1 Query: 523 IKIKYTTSPSATALQWLQPAQTSGKKHPYLFSQCQPIHARSILPCQDTPFVK 678 + I Y+T+ TALQ+L+P QT G K PY+FS+CQ IHARS +PCQDTP VK Sbjct: 104 LTILYSTTKDCTALQFLKPEQTIGGKFPYVFSECQAIHARSFIPCQDTPSVK 155 >SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe|chr 2|||Manual Length = 882 Score = 30.7 bits (66), Expect = 0.21 Identities = 21/73 (28%), Positives = 34/73 (46%), Gaps = 2/73 (2%) Frame = +2 Query: 281 HVTLSLNVDFENKVLNGSATLDVDVLQDIGDVVLDSSELTIESIELD-GAQLTYKLDDPV 457 H LSL D E G + +DVL+D + L L I + L+ G+Q + + Sbjct: 28 HYDLSLYPDLETFTYGGKVVVTLDVLEDSNSITLHGINLRILTAALEWGSQTVWASE--- 84 Query: 458 PNYG-SKLTIQLP 493 +YG ++ +Q P Sbjct: 85 VSYGDERIVLQFP 97 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +1 Query: 616 SQCQPIHARSILPCQDTPFVKFTY 687 +Q +P AR PC D P +K T+ Sbjct: 141 TQMEPTSARRAFPCWDEPALKATF 164 >SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein Mug36|Schizosaccharomyces pombe|chr 2|||Manual Length = 1646 Score = 26.6 bits (56), Expect = 3.4 Identities = 24/78 (30%), Positives = 38/78 (48%), Gaps = 5/78 (6%) Frame = +2 Query: 263 EQAVIKHVTLSLNVDFENKVLNGSATLDVDVLQDIGDVVLDSSELTIESIELDGAQLTYK 442 E + +K++TLSL V+ E V S + VD L DS+EL I+ + G+ + Sbjct: 955 EISQLKNLTLSLVVNAEEGVF--STLITVDNLDAQVQSCADSTELLIKVLSDLGSTEDEE 1012 Query: 443 LDD-----PVPNYGSKLT 481 + D P+ +Y LT Sbjct: 1013 ISDCYLALPIEDYAKSLT 1030 >SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 26.6 bits (56), Expect = 3.4 Identities = 19/74 (25%), Positives = 33/74 (44%) Frame = +2 Query: 251 LSRPEQAVIKHVTLSLNVDFENKVLNGSATLDVDVLQDIGDVVLDSSELTIESIELDGAQ 430 LS P +K + S ++ V NG +D +D + ++ S E + I LD + Sbjct: 21 LSNPS-IPLKVLNKSFRSSRQSSVSNGHGLYSLD--RDETESLMSSHEASNAGISLDSSF 77 Query: 431 LTYKLDDPVPNYGS 472 ++ P P YG+ Sbjct: 78 RVIQVGQPEPQYGN 91 >SPAC3H1.02c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 26.2 bits (55), Expect = 4.5 Identities = 22/77 (28%), Positives = 32/77 (41%), Gaps = 6/77 (7%) Frame = +1 Query: 475 IDYTIAETSLKCDKLKIKIKYTTSPSATALQWLQPAQTSGKKHPYLFSQCQPIHARSILP 654 +DYTI E LK K + +YT S S L W P+ + + C+ + ++ P Sbjct: 256 VDYTIPEPILKTVKFSSEDEYTGSVS---LAWNGPSALDAYTVFAIETLCEFLSESAVSP 312 Query: 655 CQDT------PFVKFTY 687 T PF F Y Sbjct: 313 FGQTFVEIEDPFCSFVY 329 >SPBC1778.09 |||GTPase activating protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 414 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%) Frame = +1 Query: 493 ETSLKC-DKLKIKIKYTTSPSATALQWLQPAQTSGKKHPYLFSQCQPIHAR 642 +T LK D LK ++ +T S A +P+ + + + +F QC P H + Sbjct: 282 DTLLKAFDMLKHELSFTQSGRHLAEIGAEPSAFATRWYLTVFHQCVPFHTQ 332 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,520,025 Number of Sequences: 5004 Number of extensions: 45283 Number of successful extensions: 121 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 121 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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