Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120679.seq
         (694 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr...    29   0.64 
SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces ...    29   0.84 
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch...    26   4.5  
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc...    26   4.5  
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    26   4.5  
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ...    26   5.9  
SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce...    25   7.9  
SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub...    25   7.9  
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|...    25   7.9  

>SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 454

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = +2

Query: 134 WNSIVYLEIDYENAEILSQNCVYENL 211
           W +I+  +  Y+NAE + +NC+  NL
Sbjct: 21  WQAILNYKYIYQNAEAVQRNCINRNL 46


>SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 761

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 18/51 (35%), Positives = 24/51 (47%)
 Frame = +1

Query: 325 WNLYVQLKEMRRNIYIKRRAEAEKASYDHYCDIDYCLEEERESNSKLLEVV 477
           W     LK +R    +K+R     A Y   C++  CL+EE   N  LLE V
Sbjct: 541 WKPMPDLKVVRSRPSLKQRP-LRSAEYVRVCELK-CLQEEEFDNKVLLEQV 589


>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 611

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 9/41 (21%), Positives = 23/41 (56%)
 Frame = +1

Query: 310 LAVQCWNLYVQLKEMRRNIYIKRRAEAEKASYDHYCDIDYC 432
           + +Q  N ++QL+  + N Y+++R+      +  + D ++C
Sbjct: 139 MRIQLANRHIQLRAPKYNSYLRQRSRLAYQVHSFFNDREFC 179


>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 822

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 21/72 (29%), Positives = 30/72 (41%)
 Frame = +2

Query: 8   RVNVITGLCWMLFAAFQIYVFKNGHLPTLDVLYRHYDLESVCWNSIVYLEIDYENAEILS 187
           R N ++G  W +  A    +F    L  LD    H DLE+V W    YL  + E   +L 
Sbjct: 417 RTNELSG-GWRMRIALARILFIKPTLMMLDEPTNHLDLEAVAWLE-EYLTHEMEGHTLLI 474

Query: 188 QNCVYENLYKKC 223
                + L + C
Sbjct: 475 TCHTQDTLNEVC 486


>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1364

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = +2

Query: 92   LDVLYRHYDLESVCWNSIVYLEIDYE 169
            L +L ++ +  S+CW  IVYL  D++
Sbjct: 1234 LRLLKKYLNDVSICWEQIVYLVQDFQ 1259


>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
           Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 611

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +1

Query: 205 EPLQKMHHVPSDCKGSRPTVFNQNYSVIIMGVLTI 309
           +P QK    P D K  R ++F   Y  +I+G L I
Sbjct: 48  DPFQKYR--PKDLKVQRKSIFRLRYIFLILGWLAI 80


>SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1297

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 16/43 (37%), Positives = 20/43 (46%)
 Frame = -2

Query: 303 KHAHYDYGIVLIKHGRP*SLTIARHMMHFL*RFSYTQFWLKIS 175
           KH  YDY I+L           AR  M  L  F Y+ F L++S
Sbjct: 349 KHEFYDYNIILFFQNS------AREYMFSLTDFFYSDFELRVS 385


>SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory
           subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 838

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/44 (31%), Positives = 23/44 (52%)
 Frame = +1

Query: 367 YIKRRAEAEKASYDHYCDIDYCLEEERESNSKLLEVVSEGRNSS 498
           Y++   E E    ++  ++ + LE+E ESNS   +VV   R  S
Sbjct: 665 YLEDMDEGEMLDANNLPEMQFALEQELESNSSDDDVVEVHRELS 708


>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
           Alp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 784

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/35 (37%), Positives = 23/35 (65%)
 Frame = +1

Query: 406 DHYCDIDYCLEEERESNSKLLEVVSEGRNSSSVAA 510
           +H   +D CL+E   +NS+LL+V S+  N+ ++ A
Sbjct: 680 EHIDFLDTCLKECMLTNSRLLKVQSKLLNTCAMFA 714


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,562,797
Number of Sequences: 5004
Number of extensions: 49323
Number of successful extensions: 150
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)