Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120677.seq
(691 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB795E Cluster: PREDICTED: similar to tRNA-splic... 56 8e-07
UniRef50_UPI00015B4278 Cluster: PREDICTED: similar to CG7023-PA;... 54 4e-06
UniRef50_UPI0000ECA582 Cluster: tRNA-splicing endonuclease subun... 47 5e-04
UniRef50_A7RL81 Cluster: Predicted protein; n=1; Nematostella ve... 46 7e-04
UniRef50_Q7Z6J9 Cluster: tRNA-splicing endonuclease subunit Sen5... 45 0.002
UniRef50_UPI0000E46005 Cluster: PREDICTED: similar to zinc finge... 44 0.004
UniRef50_Q9VTV4 Cluster: CG5626-PA; n=2; Sophophora|Rep: CG5626-... 44 0.004
UniRef50_Q6DE24 Cluster: MGC80279 protein; n=3; Xenopus|Rep: MGC... 40 0.043
UniRef50_Q16F18 Cluster: Putative uncharacterized protein; n=2; ... 40 0.076
UniRef50_Q504E6 Cluster: Zgc:109927; n=4; Clupeocephala|Rep: Zgc... 35 2.2
UniRef50_Q4SAU2 Cluster: Chromosome 3 SCAF14679, whole genome sh... 35 2.2
UniRef50_Q48537 Cluster: DNA primase; n=1; Lactobacillus delbrue... 34 3.8
UniRef50_A3CT74 Cluster: TRNA intron endonuclease; n=3; Methanom... 34 3.8
UniRef50_Q7RF07 Cluster: Putative uncharacterized protein PY0490... 33 6.6
UniRef50_Q4MZ65 Cluster: Putative uncharacterized protein; n=2; ... 33 6.6
UniRef50_UPI0000499565 Cluster: phosphoinositide kinase; n=1; En... 33 8.7
UniRef50_A1VEN8 Cluster: Putative uncharacterized protein; n=2; ... 33 8.7
>UniRef50_UPI0000DB795E Cluster: PREDICTED: similar to tRNA-splicing
endonuclease subunit Sen54 (tRNA-intron endonuclease
Sen54); n=1; Apis mellifera|Rep: PREDICTED: similar to
tRNA-splicing endonuclease subunit Sen54 (tRNA-intron
endonuclease Sen54) - Apis mellifera
Length = 547
Score = 56.0 bits (129), Expect = 8e-07
Identities = 25/56 (44%), Positives = 38/56 (67%)
Frame = +3
Query: 510 VIRQTGGYWQFYGHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILLN 677
V + +G W +G + LYL PEEALFL+E+NCL L N ++S+Q+AY +L++
Sbjct: 95 VKKYSGQDWSSFGLEKSGILYLIPEEALFLLETNCLELTWNDVSLSIQQAYELLID 150
Score = 46.4 bits (105), Expect = 7e-04
Identities = 19/48 (39%), Positives = 30/48 (62%)
Frame = +1
Query: 343 LPIIGLKEVVPNGSWLEQKQIQVALEARKHLIEVNRIEKQGSLSHAEW 486
LP +K+ PN SWL+ QI+ + RK+LI + R+++ L+ AEW
Sbjct: 39 LPKSEMKQFEPNNSWLQNMQIEKGIITRKNLIAIERVDRISQLASAEW 86
>UniRef50_UPI00015B4278 Cluster: PREDICTED: similar to CG7023-PA;
n=1; Nasonia vitripennis|Rep: PREDICTED: similar to
CG7023-PA - Nasonia vitripennis
Length = 687
Score = 53.6 bits (123), Expect = 4e-06
Identities = 24/53 (45%), Positives = 36/53 (67%)
Frame = +1
Query: 328 RTEGALPIIGLKEVVPNGSWLEQKQIQVALEARKHLIEVNRIEKQGSLSHAEW 486
++E ALP IG K P+GSWL+ Q++ L+ RK LIE+ R+++ L+ AEW
Sbjct: 34 QSELALPKIGKKHFEPSGSWLQDMQLENGLKNRKRLIELERVDRISQLAIAEW 86
Score = 44.8 bits (101), Expect = 0.002
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +3
Query: 510 VIRQTGGYWQFYGHHQGKKLYLKPEEALFLMESNCLRL 623
V +++G W+ +GH +YL PEEAL L+E NCL L
Sbjct: 95 VTKRSGQKWESFGHEDRCNMYLLPEEALLLLEMNCLEL 132
>UniRef50_UPI0000ECA582 Cluster: tRNA-splicing endonuclease subunit
Sen54 (tRNA-intron endonuclease Sen54) (HsSen54).; n=4;
Gallus gallus|Rep: tRNA-splicing endonuclease subunit
Sen54 (tRNA-intron endonuclease Sen54) (HsSen54). -
Gallus gallus
Length = 530
Score = 46.8 bits (106), Expect = 5e-04
Identities = 23/55 (41%), Positives = 33/55 (60%)
Frame = +3
Query: 525 GGYWQFYGHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILLNEELL 689
G +W G + K L PEEAL+L+E L+L MS++EAY ILL++E +
Sbjct: 95 GKFWHTMGFSERGKQCLLPEEALYLLECGSLQLFYKDVPMSVREAYEILLSQEAM 149
Score = 39.9 bits (89), Expect = 0.057
Identities = 21/72 (29%), Positives = 38/72 (52%)
Frame = +1
Query: 295 SGEELVAKGVTRTEGALPIIGLKEVVPNGSWLEQKQIQVALEARKHLIEVNRIEKQGSLS 474
S EL A R+ + G KE VP+GS + +++++ LE + + R+E+ G+L
Sbjct: 18 SSGELQAVSYRRSRASWRSCGQKEFVPDGSTEQAERLRLCLEEQWRQLAEERVERPGNLV 77
Query: 475 HAEWRNDLRLAE 510
A W+ + + E
Sbjct: 78 KAVWKPEQSVVE 89
>UniRef50_A7RL81 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 326
Score = 46.4 bits (105), Expect = 7e-04
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = +3
Query: 525 GGYWQFYGHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILL 674
G +W +G L+ PEEALFL+E L L G ++LQ+A+SILL
Sbjct: 85 GTHWNHFGVTINSVLWAAPEEALFLLEQGSLELYYGGMPLTLQQAFSILL 134
>UniRef50_Q7Z6J9 Cluster: tRNA-splicing endonuclease subunit Sen54;
n=23; Mammalia|Rep: tRNA-splicing endonuclease subunit
Sen54 - Homo sapiens (Human)
Length = 526
Score = 45.2 bits (102), Expect = 0.002
Identities = 20/52 (38%), Positives = 31/52 (59%)
Frame = +3
Query: 525 GGYWQFYGHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILLNE 680
G +WQ G + + L PEEAL+L+E + L + +S+QEAY +LL +
Sbjct: 96 GKFWQTMGFSEQGRQRLHPEEALYLLECGSIHLFHQDLPLSIQEAYQLLLTD 147
Score = 36.7 bits (81), Expect = 0.53
Identities = 22/72 (30%), Positives = 37/72 (51%)
Frame = +1
Query: 274 MPDPKLLSGEELVAKGVTRTEGALPIIGLKEVVPNGSWLEQKQIQVALEARKHLIEVNRI 453
+P ++LS EL A + G K+ +P+GS + ++++ E L+ R+
Sbjct: 12 VPAGRVLSARELFAARSRSQKLPQRSHGPKDFLPDGSAAQAERLRRCREELWQLLAEQRV 71
Query: 454 EKQGSLSHAEWR 489
E+ GSL AEWR
Sbjct: 72 ERLGSLVAAEWR 83
>UniRef50_UPI0000E46005 Cluster: PREDICTED: similar to zinc finger
protein; n=3; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to zinc finger protein -
Strongylocentrotus purpuratus
Length = 1060
Score = 44.0 bits (99), Expect = 0.004
Identities = 21/51 (41%), Positives = 33/51 (64%), Gaps = 1/51 (1%)
Frame = +3
Query: 525 GGYWQFYGHH-QGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILL 674
G +W+ G+ QG+K YL PEE LFL+E + L G +S+Q AY++++
Sbjct: 447 GKFWRTMGYMLQGQK-YLYPEEGLFLLEVGSMELQYGGTPLSVQRAYNLMI 496
>UniRef50_Q9VTV4 Cluster: CG5626-PA; n=2; Sophophora|Rep: CG5626-PA
- Drosophila melanogaster (Fruit fly)
Length = 383
Score = 44.0 bits (99), Expect = 0.004
Identities = 20/56 (35%), Positives = 37/56 (66%)
Frame = +3
Query: 513 IRQTGGYWQFYGHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILLNE 680
+++ G ++ +G+ + KLYL+ EA+FL+E L+L G +S+++AY +LL E
Sbjct: 91 VKRKDGKFENFGYSEQGKLYLEYYEAMFLLEVGRLQLEYCGLVVSIEQAYVLLLGE 146
Score = 37.1 bits (82), Expect = 0.40
Identities = 25/78 (32%), Positives = 39/78 (50%)
Frame = +1
Query: 277 PDPKLLSGEELVAKGVTRTEGALPIIGLKEVVPNGSWLEQKQIQVALEARKHLIEVNRIE 456
P LS +EL+A TE P GLK G+ E ++++ A E + + V RIE
Sbjct: 15 PKNSYLSAQELIAH--RHTEFEPPSGGLKRTKNEGTAAEVEELKRAQEYLRAQLSVPRIE 72
Query: 457 KQGSLSHAEWRNDLRLAE 510
+ G + A W + ++AE
Sbjct: 73 RLGGRALAIWNEEQQVAE 90
>UniRef50_Q6DE24 Cluster: MGC80279 protein; n=3; Xenopus|Rep:
MGC80279 protein - Xenopus laevis (African clawed frog)
Length = 517
Score = 40.3 bits (90), Expect = 0.043
Identities = 21/54 (38%), Positives = 33/54 (61%), Gaps = 1/54 (1%)
Frame = +3
Query: 525 GGYWQFYGH-HQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILLNEE 683
G +WQ G +QGK+ L PEEA++L+E +++ +S+QEAY LL +
Sbjct: 80 GKFWQTMGFTYQGKQCLL-PEEAVYLLECGAVQIFYRDSPLSVQEAYERLLGNQ 132
Score = 38.7 bits (86), Expect = 0.13
Identities = 24/76 (31%), Positives = 44/76 (57%), Gaps = 2/76 (2%)
Frame = +1
Query: 289 LLSGEELVAKGVTRTEGALPII--GLKEVVPNGSWLEQKQIQVALEARKHLIEVNRIEKQ 462
+LS +EL+A E +LP + G K+ + +G ++++++QV L + L++ R+E+
Sbjct: 1 MLSPKELLA--ARNREKSLPQLSHGQKDFIADGLEIQKEKLQVCLAEQWELLKEERVERL 58
Query: 463 GSLSHAEWRNDLRLAE 510
GSL + WR L E
Sbjct: 59 GSLVKSVWRPKEDLVE 74
>UniRef50_Q16F18 Cluster: Putative uncharacterized protein; n=2;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 418
Score = 39.5 bits (88), Expect = 0.076
Identities = 20/54 (37%), Positives = 32/54 (59%)
Frame = +3
Query: 513 IRQTGGYWQFYGHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILL 674
IR+ G W YG+ + K Y+ EAL L+E N L + + +SL++AY++ L
Sbjct: 88 IRKAVGKWHIYGYLENKVQYVDGYEALHLLEMNRLIVFWDTVLISLEQAYTLFL 141
>UniRef50_Q504E6 Cluster: Zgc:109927; n=4; Clupeocephala|Rep:
Zgc:109927 - Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 397
Score = 34.7 bits (76), Expect = 2.2
Identities = 16/46 (34%), Positives = 27/46 (58%)
Frame = +3
Query: 546 GHHQGKKLYLKPEEALFLMESNCLRLNNNGXTMSLQEAYSILLNEE 683
G + K YL+PEEAL+LME +++ +S+Q+ Y L+ +
Sbjct: 2 GFSERGKQYLQPEEALYLMECGNVQVFYRDLPLSIQDGYERFLSSD 47
>UniRef50_Q4SAU2 Cluster: Chromosome 3 SCAF14679, whole genome
shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 3
SCAF14679, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 283
Score = 34.7 bits (76), Expect = 2.2
Identities = 14/48 (29%), Positives = 29/48 (60%)
Frame = +1
Query: 343 LPIIGLKEVVPNGSWLEQKQIQVALEARKHLIEVNRIEKQGSLSHAEW 486
+P+ G K+ P+ S ++++++ +L L+ R+E+ GSL A+W
Sbjct: 27 IPVRGQKDFFPDNSEQQKRRLEQSLSEHWSLLAEERVERLGSLVKAKW 74
>UniRef50_Q48537 Cluster: DNA primase; n=1; Lactobacillus
delbrueckii|Rep: DNA primase - Lactobacillus delbrueckii
Length = 598
Score = 33.9 bits (74), Expect = 3.8
Identities = 14/32 (43%), Positives = 24/32 (75%)
Frame = +2
Query: 209 IIEGMLNFPNFKTSTFSNIGKQCLIQNSYQEK 304
II+ M NFP FK +TF+N+ +Q +I+ +++ K
Sbjct: 397 IIQSMNNFPKFKDTTFANLRRQRVIKFNHEFK 428
>UniRef50_A3CT74 Cluster: TRNA intron endonuclease; n=3;
Methanomicrobiales|Rep: TRNA intron endonuclease -
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498
/ JR1)
Length = 336
Score = 33.9 bits (74), Expect = 3.8
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = +3
Query: 543 YGHHQGKKLYLKPEEALFLMESNCLRLNNNG 635
YG +G+ L L PEEAL+LME N + + + G
Sbjct: 25 YGRPEGRGLRLSPEEALYLMERNKIDVKDFG 55
>UniRef50_Q7RF07 Cluster: Putative uncharacterized protein PY04904;
n=2; Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein PY04904 - Plasmodium yoelii yoelii
Length = 347
Score = 33.1 bits (72), Expect = 6.6
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
Frame = +3
Query: 18 HSFLIYVFCNH*EMIMNELIIF-YSKN*LKNFFYLLIEKCYKIIVGKMYFSSVTTYLKLK 194
H I+ FC + ++ NE + KN +K F+Y + KC +I+ +YF
Sbjct: 54 HIAYIFYFCEY--ILKNENTLHTQKKNFIKCFYYTMPNKCLYLILNSLYFGKKNHKYVQC 111
Query: 195 SEIIELLRVC 224
E+I + +C
Sbjct: 112 VEVITNIFIC 121
>UniRef50_Q4MZ65 Cluster: Putative uncharacterized protein; n=2;
Theileria|Rep: Putative uncharacterized protein -
Theileria parva
Length = 647
Score = 33.1 bits (72), Expect = 6.6
Identities = 19/59 (32%), Positives = 32/59 (54%)
Frame = -3
Query: 182 IGSHRREIHFTYNNFITFLN*EVEEIFKLILGIKDN*FIHNHFLMIAKHVNKKTMNRSS 6
+ SH + IHFT+N F T+ + E+E++ K D F+ N ++ VN+ + SS
Sbjct: 536 VNSHYKSIHFTFNKFTTYED-ELEKLLKSSNLDIDTSFVINVTNVLMSKVNESCLYLSS 593
>UniRef50_UPI0000499565 Cluster: phosphoinositide kinase; n=1;
Entamoeba histolytica HM-1:IMSS|Rep: phosphoinositide
kinase - Entamoeba histolytica HM-1:IMSS
Length = 714
Score = 32.7 bits (71), Expect = 8.7
Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = +3
Query: 576 KPEEALFLMESNCLRLNNNGXTMSLQEA-YSILLNEEL 686
KPEE+L L+ CL+ + +L+E+ YSI LNE L
Sbjct: 110 KPEESLKLIRMRCLKCQKSSEVWTLRESIYSISLNELL 147
>UniRef50_A1VEN8 Cluster: Putative uncharacterized protein; n=2;
Desulfovibrio vulgaris subsp. vulgaris|Rep: Putative
uncharacterized protein - Desulfovibrio vulgaris subsp.
vulgaris (strain DP4)
Length = 178
Score = 32.7 bits (71), Expect = 8.7
Identities = 17/53 (32%), Positives = 31/53 (58%)
Frame = -3
Query: 554 MMSIELPVSTSLSNYSASLKSFLHSACDKLPCFSILLTSIKCFLASKATCICF 396
++ + + S++LS Y+ SLK L S+ L F +L+ SI+ + +CIC+
Sbjct: 49 VIPLVMAASSALS-YNVSLKETLLSSVIALSLFLLLVLSIRMIVCMNISCICY 100
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 622,109,948
Number of Sequences: 1657284
Number of extensions: 12073117
Number of successful extensions: 27154
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 26377
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27141
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 54132236449
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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