Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120652.seq
(692 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 3.9
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 3.9
At4g05300.1 68417.m00803 hypothetical protein 29 3.9
At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 28 5.1
At5g43490.1 68418.m05317 hypothetical protein similar to unknown... 28 6.8
At5g08780.1 68418.m01041 histone H1/H5 family protein contains P... 28 6.8
At5g08630.1 68418.m01026 DDT domain-containing protein low simil... 28 6.8
At4g25320.1 68417.m03643 DNA-binding protein-related contains Pf... 28 6.8
At1g77060.1 68414.m08972 mutase family protein similar to carbox... 28 6.8
At1g70420.1 68414.m08102 expressed protein 28 6.8
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 28 6.8
At5g35695.1 68418.m04268 hypothetical protein 27 8.9
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 28.7 bits (61), Expect = 3.9
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +1
Query: 58 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 198
Q G F DT +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 28.7 bits (61), Expect = 3.9
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +1
Query: 58 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 198
Q G F DT +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At4g05300.1 68417.m00803 hypothetical protein
Length = 387
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/59 (30%), Positives = 24/59 (40%)
Frame = +2
Query: 503 RWQRRFGGSKPIAHFVCQRNDCGSTRR*NGSARLRKRAPRNGALGQPHGGHCARRDSQT 679
R R G ++PI + GS RR + R+ R G +GQ RD QT
Sbjct: 237 RTDRWIGSARPIVRRIGSARRIGSARRIGSARRIGLTDRRTGQMGQ--ADRLTERDGQT 293
>At2g02790.1 68415.m00222 calmodulin-binding family protein very low
similarity to SP|P12036 Neurofilament triplet H protein
{Homo sapiens}; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 636
Score = 28.3 bits (60), Expect = 5.1
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Frame = +2
Query: 185 NGQYVSTSTASTNRRLSMPTKSSTCSR*RG--KAGR--PAVFAPTHSAHYQI 328
NG S +T ++RR S+P K + G ++GR P+ APT SA +I
Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523
>At5g43490.1 68418.m05317 hypothetical protein similar to unknown
protein (gb|AAF20218.1)
Length = 131
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
Frame = +3
Query: 237 CRPNPAHAPDSVA--KQGDPLYLHPHTVLITKSGVIQLIMKSKLPXAIELQEWLL 395
C P+P A V + G+P PH L + ++ + + LP + QEWLL
Sbjct: 33 CPPSPLAATMKVKVKENGEPSAKPPHPDLKYLNQILHVPTRELLPEIDDDQEWLL 87
>At5g08780.1 68418.m01041 histone H1/H5 family protein contains Pfam
domain, PF00538: linker histone H1 and H5 family
Length = 457
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/48 (33%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +1
Query: 184 ERAIRVHVDGKYKST-FEHADQIQHMLQIAWQSRATRCICTHTQCSLP 324
E AI + KYK+ F H + + H L + R C C + SLP
Sbjct: 77 EDAISEFIKSKYKNLPFAHTNLLSHHLAKLVEKREILCDCNNDCYSLP 124
>At5g08630.1 68418.m01026 DDT domain-containing protein low
similarity to SP|Q9NRL2 Bromodomain adjacent to zinc
finger domain protein 1A (ATP-utilizing chromatin
assembly and remodeling factor 1) (ATP-dependent
chromatin remodelling protein) (Williams syndrome
transcription factor-related chromatin remodeling factor
180) {Homo sapiens}; contains Pfam profile PF02791: DDT
domain
Length = 723
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/42 (28%), Positives = 24/42 (57%)
Frame = +1
Query: 376 NYKNGFWRRSFLKCYARXKYAPAVEMDTNDVIAKIDDLTQKL 501
++K + R+FLK + R ++ ND +AK ++T+K+
Sbjct: 170 SWKKFPFSRNFLKSFIRDSTCHSIPWVVNDYLAKAHEITRKI 211
>At4g25320.1 68417.m03643 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 404
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +2
Query: 203 TSTASTNRRLSMPTKSSTCSR*RGKAGRPAVFAP 304
T A+T L+MPT++++ + + K GRP + P
Sbjct: 64 TENAATPFSLTMPTENTSAEQLKKKRGRPRKYNP 97
>At1g77060.1 68414.m08972 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 339
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +3
Query: 249 PAHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKS 356
P H PD + + G L +HP T L + + ++K+
Sbjct: 256 PLHTPDELKEMGFHLIVHPLTALYASTRALVDVLKT 291
>At1g70420.1 68414.m08102 expressed protein
Length = 272
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +2
Query: 185 NGQYVSTSTASTNRRLSMPTKSSTCSR*RGKAGRPAVFAPTHSAHYQIW 331
NG ++ST+ST RLS KSS + + K + T SAH +++
Sbjct: 187 NGSSSTSSTSSTAARLSGVVKSSEKGKEKTKTDKKKDKMRTKSAHEKLY 235
>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
similar to GTL1 [Arabidopsis thaliana] GI:2664198
Length = 669
Score = 27.9 bits (59), Expect = 6.8
Identities = 21/54 (38%), Positives = 26/54 (48%)
Frame = -2
Query: 658 AMSAMRLAQCAVSRXAFSQSCRAVSASRRATIISLANKMSDRFASAKSALPPSI 497
A +A+ A SQ RA SASR A IISL K++ LPPS+
Sbjct: 305 AWKRQEMARLAREHEVMSQE-RAASASRDAAIISLIQKITGHTIQ----LPPSL 353
>At5g35695.1 68418.m04268 hypothetical protein
Length = 211
Score = 27.5 bits (58), Expect = 8.9
Identities = 20/58 (34%), Positives = 26/58 (44%)
Frame = -1
Query: 668 HVLRNVRHAVGPVRRFAXRVFAILPSRFSVASSHNHFVGKQNERSVCFRQICVATVNF 495
H L N+RH +R R+F I SRF++ S F K+ V C A NF
Sbjct: 87 HELFNLRHV--SLRNVIERIFGIFKSRFAIFKSAPPFSYKKQAGLVL---TCAALHNF 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,701,496
Number of Sequences: 28952
Number of extensions: 336117
Number of successful extensions: 851
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 851
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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