BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120621.seq (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 30 0.36 SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce... 28 1.1 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 5.9 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 29.9 bits (64), Expect = 0.36 Identities = 13/35 (37%), Positives = 23/35 (65%) Frame = -1 Query: 657 IFKSVFNKYSFLQI*HRI*IYFPMCKSVIKRSLQK 553 +FK + Y+FL + + + I FP+ S +K+SL+K Sbjct: 61 LFKVINTTYTFLYLRNSLTITFPLLNSSVKQSLKK 95 >SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1418 Score = 28.3 bits (60), Expect = 1.1 Identities = 22/51 (43%), Positives = 27/51 (52%) Frame = -1 Query: 360 LAILKNY*INKKLIRTSAFRLRTRKIHFTYL*SK*LNSTNTY*HYIIPIVC 208 L+IL NY K IR FR R R +F YL S L+ T H+I +VC Sbjct: 303 LSILVNY---CKSIRFRCFRCR-RVEYFFYLDSNPLSIQRTITHFIKKLVC 349 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/36 (25%), Positives = 20/36 (55%) Frame = -1 Query: 255 LNSTNTY*HYIIPIVCMSTLLSEEVHNHYYSAINLL 148 +N+ TY H+++P++ + + + + Y NLL Sbjct: 406 MNARRTYVHWVVPLIDIKMEARQSIADLSYKITNLL 441 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,567,054 Number of Sequences: 5004 Number of extensions: 48492 Number of successful extensions: 90 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 87 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 90 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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