BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120598.Seq (733 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 31 0.13 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 30 0.39 SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo... 29 0.68 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 28 1.2 SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|ch... 27 2.8 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 3.6 SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces p... 26 4.8 SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 26 4.8 SPBC342.06c |rtt109|kat11|RTT109 family histone lysine acetyltra... 25 8.4 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 25 8.4 SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 25 8.4 >SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 744 Score = 31.5 bits (68), Expect = 0.13 Identities = 24/81 (29%), Positives = 39/81 (48%), Gaps = 2/81 (2%) Frame = -2 Query: 486 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 307 P+ + L L+S A R T L + A G + + PR++L V EP+ + S Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442 Query: 306 SID--SGFSAIYIWLNLLSRV 250 S++ SGFS L+ + +V Sbjct: 443 SLNNFSGFSTKKDVLHAIEKV 463 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 29.9 bits (64), Expect = 0.39 Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = -1 Query: 652 ELPRFSCTPECSW-ICAIRAPLVRTAAPCWGRSSTQSCCRLR 530 ++ RFS SW + +AP V + + C S ++SCC LR Sbjct: 1769 DIDRFSLKMLESWGLFENKAPFVNSTSICTAVSESRSCCHLR 1810 >SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 385 Score = 29.1 bits (62), Expect = 0.68 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%) Frame = -1 Query: 445 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 332 K+CG A HGH R R+W F + Y +K ++ F Sbjct: 39 KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 28.3 bits (60), Expect = 1.2 Identities = 17/48 (35%), Positives = 26/48 (54%) Frame = -2 Query: 384 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSAIYIWLNLLSRVRCA 241 LTP +P+ ++L+P L+ L S+I SG I ++ L VR A Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSGSIHSDIRVSQLREVRAA 720 >SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/42 (30%), Positives = 18/42 (42%) Frame = -1 Query: 598 APLVRTAAPCWGRSSTQSCCRLRIATCHCPYSFSGRYTFEYN 473 A +R W R+ C L+I T P F+GR Y+ Sbjct: 12 AKKLRNGTKVWARAGYFKCKLLKITTTGAPCLFAGRLKRRYD 53 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 26.6 bits (56), Expect = 3.6 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +2 Query: 305 DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 430 D I + G NK S+ + T+ + SPN+ +T+SN T+ Sbjct: 891 DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932 >SPBP8B7.29 |||para-aminobenzoate synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 718 Score = 26.2 bits (55), Expect = 4.8 Identities = 12/38 (31%), Positives = 17/38 (44%) Frame = -1 Query: 595 PLVRTAAPCWGRSSTQSCCRLRIATCHCPYSFSGRYTF 482 PL T + WG S C ++ C +SF G+ F Sbjct: 399 PLNETCSEWWGELLA-STCNTKLDNLSCLHSFDGKQNF 435 >SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 26.2 bits (55), Expect = 4.8 Identities = 14/52 (26%), Positives = 26/52 (50%) Frame = +2 Query: 308 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 463 D +KE + K +S+F ++Y PNA+ N T ++D++E + Sbjct: 64 DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112 >SPBC342.06c |rtt109|kat11|RTT109 family histone lysine acetyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 369 Score = 25.4 bits (53), Expect = 8.4 Identities = 8/18 (44%), Positives = 13/18 (72%) Frame = -1 Query: 652 ELPRFSCTPECSWICAIR 599 ELPRF P+C ++C ++ Sbjct: 212 ELPRFPDDPKCRYLCELQ 229 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 25.4 bits (53), Expect = 8.4 Identities = 14/34 (41%), Positives = 21/34 (61%) Frame = -2 Query: 432 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 331 P V++ V AF +TP YTVP+ +++PTL Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415 >SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1562 Score = 25.4 bits (53), Expect = 8.4 Identities = 18/66 (27%), Positives = 31/66 (46%) Frame = +2 Query: 227 PGGQFAHRTRDNKLSQMYIAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAAST 406 P G + KL + E PLS +D++ GSN T+ +F ++ G +S + Sbjct: 500 PNGDLYNFNGSMKLDSIQ-GEIPLSNNDVLYRGSNLRNTSELFALVIFT-GEESKIRMNA 557 Query: 407 SSNVTM 424 NV++ Sbjct: 558 VRNVSV 563 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,041,481 Number of Sequences: 5004 Number of extensions: 62463 Number of successful extensions: 171 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 169 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 345237368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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