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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120598.Seq
         (733 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g30260.1 68414.m03701 expressed protein                             35   0.064
At2g36810.1 68415.m04514 expressed protein                             31   0.79 
At3g13920.1 68416.m01758 eukaryotic translation initiation facto...    30   1.8  
At2g28310.2 68415.m03438 expressed protein contains Pfam profile...    29   3.2  
At2g28310.1 68415.m03437 expressed protein contains Pfam profile...    29   3.2  
At5g23890.1 68418.m02806 expressed protein weak similarity to SP...    28   7.3  
At1g54270.1 68414.m06187 eukaryotic translation initiation facto...    28   7.3  
At1g16970.1 68414.m02061 Ku70-like protein identical to Ku70-lik...    27   9.7  

>At1g30260.1 68414.m03701 expressed protein
          Length = 97

 Score = 34.7 bits (76), Expect = 0.064
 Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 7/66 (10%)
 Frame = +2

Query: 308 DIVKEGSNKVGTNSIFLGTVYDYGVKSP-------NAASTSSNVTMTRGTANFDIKEFKS 466
           D +KE   +VGT+SIF  +     + SP       + +S S++ + T G   F + E + 
Sbjct: 31  DTIKEEEREVGTDSIFPSSFNSKKISSPFTSPYSSSVSSASASASCTSGLNKFPVTENRG 90

Query: 467 MFIVFK 484
            F VFK
Sbjct: 91  SFPVFK 96


>At2g36810.1 68415.m04514 expressed protein 
          Length = 1071

 Score = 31.1 bits (67), Expect = 0.79
 Identities = 15/35 (42%), Positives = 23/35 (65%)
 Frame = +2

Query: 5   NICTT*ISLELKL*IKTNHVENRVIDRFARTDLSL 109
           N CTT +S+E KL I+T +   +++D+F    LSL
Sbjct: 616 NACTTLVSVEPKLTIETRNRVMKILDQFFSISLSL 650


>At3g13920.1 68416.m01758 eukaryotic translation initiation factor
           4A-1 / eIF-4A-1 eIF-4A-1 gi:15293046, gi:15450485;
           contains Pfam profile PF00270: DEAD/DEAH box helicase;
           contains Pfam profile PF00271: Helicase conserved
           C-terminal domain
          Length = 412

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 21/70 (30%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
 Frame = +2

Query: 230 GGQFAHRTRDNKLSQMYIAEKPL--SIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAAS 403
           G QF  R  D KL+++   +     S DD V E  + +G     L  +Y YG + P+A  
Sbjct: 8   GTQFDARQFDQKLNEVLEGQDEFFTSYDD-VHESFDAMGLQENLLRGIYAYGFEKPSAIQ 66

Query: 404 TSSNVTMTRG 433
               V   +G
Sbjct: 67  QRGIVPFCKG 76


>At2g28310.2 68415.m03438 expressed protein contains Pfam profile
           PF05212: Protein of unknown function (DUF707)
          Length = 374

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 24/81 (29%), Positives = 42/81 (51%), Gaps = 8/81 (9%)
 Frame = -2

Query: 516 IVLTVLVGVIPLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKM--LL 343
           +++T ++G++    I + L SL  K  +P  +++ LDV A  GD +P  +   RK   + 
Sbjct: 19  VIITTILGIVFGTFIGITLPSLSFKINLPSRLISSLDV-AISGDKSPEDFG-SRKFPEIY 76

Query: 342 VPT------LLEPSLTMSSID 298
           VPT      LL P + ++  D
Sbjct: 77  VPTNPRGAELLPPGIVVAKTD 97


>At2g28310.1 68415.m03437 expressed protein contains Pfam profile
           PF05212: Protein of unknown function (DUF707)
          Length = 374

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 24/81 (29%), Positives = 42/81 (51%), Gaps = 8/81 (9%)
 Frame = -2

Query: 516 IVLTVLVGVIPLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKM--LL 343
           +++T ++G++    I + L SL  K  +P  +++ LDV A  GD +P  +   RK   + 
Sbjct: 19  VIITTILGIVFGTFIGITLPSLSFKINLPSRLISSLDV-AISGDKSPEDFG-SRKFPEIY 76

Query: 342 VPT------LLEPSLTMSSID 298
           VPT      LL P + ++  D
Sbjct: 77  VPTNPRGAELLPPGIVVAKTD 97


>At5g23890.1 68418.m02806 expressed protein weak similarity to
           SP|P12957 Caldesmon (CDM) {Gallus gallus}
          Length = 946

 Score = 27.9 bits (59), Expect = 7.3
 Identities = 14/39 (35%), Positives = 22/39 (56%)
 Frame = +3

Query: 114 TNALNLNSLTEASPSLGQSSESVESDENKRLNVKLNNAR 230
           T    ++SLT    S+ QSS+ + SDE K  N + +N +
Sbjct: 120 TKKQEMHSLTSQQESMIQSSDEISSDEIKVANSEESNLK 158


>At1g54270.1 68414.m06187 eukaryotic translation initiation factor
           4A-2 / eIF-4A-2 similar to eukaryotic translation
           initiation factor 4A GI:19696 from [Nicotiana
           plumbaginifolia]
          Length = 412

 Score = 27.9 bits (59), Expect = 7.3
 Identities = 18/69 (26%), Positives = 29/69 (42%), Gaps = 1/69 (1%)
 Frame = +2

Query: 230 GGQFAHRTRDNKLSQMYIAEKPLSID-DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAAST 406
           G QF  R  D +L+++   +       D V E  + +G     L  +Y YG + P+A   
Sbjct: 8   GTQFDTRQFDQRLNEVLDGQDEFFTSYDEVHESFDAMGLQENLLRGIYAYGFEKPSAIQQ 67

Query: 407 SSNVTMTRG 433
              V   +G
Sbjct: 68  RGIVPFCKG 76


>At1g16970.1 68414.m02061 Ku70-like protein identical to Ku70-like
           protein GI:12006424 from [Arabidopsis thaliana];
           contains Pfam profiles PF03731: Ku70/Ku80 N-terminal
           alpha/beta domain, PF02735: Ku70/Ku80 beta-barrel
           domain, PF03730: Ku70/Ku80 C-terminal arm, and PF02037:
           SAP domain; contains TIGRfam profile TIGR00578:
           ATP-dependent DNA helicase ii, 70 kDa subunit
          Length = 621

 Score = 27.5 bits (58), Expect = 9.7
 Identities = 13/31 (41%), Positives = 18/31 (58%)
 Frame = +3

Query: 123 LNLNSLTEASPSLGQSSESVESDENKRLNVK 215
           LN + LTE  PS+GQ  E ++    KR+  K
Sbjct: 234 LNSDELTEFMPSVGQKLEDMKDQLKKRVLAK 264


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,727,640
Number of Sequences: 28952
Number of extensions: 326552
Number of successful extensions: 869
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 849
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 869
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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