BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120590.Seq (723 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1399.03 |fur4||uracil permease|Schizosaccharomyces pombe|chr... 28 1.6 SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 27 2.1 SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 2.1 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.1 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 27 3.6 SPBC21H7.04 |||ATP-dependent RNA helicase Dbp7 |Schizosaccharomy... 26 4.7 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 8.3 SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 25 8.3 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 25 8.3 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 8.3 >SPAC1399.03 |fur4||uracil permease|Schizosaccharomyces pombe|chr 1|||Manual Length = 581 Score = 27.9 bits (59), Expect = 1.6 Identities = 15/47 (31%), Positives = 20/47 (42%) Frame = -1 Query: 324 LNHARFGNEHCVWVQIQRVALLCPLSGAWCWIWSACSNVDLYLPSTW 184 L+ + FG +W + R L C G WI C V L + S W Sbjct: 123 LSRSSFGTWGSLWPILNRSVLACVWYGVQAWIGGEC--VVLMIRSIW 167 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 27.5 bits (58), Expect = 2.1 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = +2 Query: 446 DVIAKIDDLTQKLTVANAD 502 D +KID LT+KL VANA+ Sbjct: 618 DSASKIDSLTEKLKVANAE 636 >SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 728 Score = 27.5 bits (58), Expect = 2.1 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +1 Query: 298 LITKSGVIQLIMKSKLPYAIELQEWLL 378 L+ K GV+ L+ K KLP+++ ++ ++ Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 27.5 bits (58), Expect = 2.1 Identities = 33/108 (30%), Positives = 49/108 (45%), Gaps = 4/108 (3%) Frame = -3 Query: 721 ENSLLPTS--HTARLWHSCGLLGLAITSCAMSAMRLASCAVLRRAFSQSCRAVSASRRAT 548 EN ++ +S HTAR+W L TS A + M + R S +V S + Sbjct: 313 ENYIISSSRDHTARVWR------LDATSPAEACMHVL------RGHLASVNSVQYSSKTG 360 Query: 547 IISLANKMSDRLLR--QICVGHRQFLRQIVNFGNNIICIHFNGRRVLA 410 +I A+ SDR LR I GH +R I I C +NG+ +++ Sbjct: 361 LIVTAS--SDRTLRTWDITTGH--CIRIIHAHQRGIACAQYNGKFIVS 404 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 26.6 bits (56), Expect = 3.6 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +3 Query: 534 ANEMIVARRDAETARQDCENARRKTAQLAN 623 ANE + +RD R+D + +RK QL N Sbjct: 334 ANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363 >SPBC21H7.04 |||ATP-dependent RNA helicase Dbp7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 709 Score = 26.2 bits (55), Expect = 4.7 Identities = 15/49 (30%), Positives = 28/49 (57%), Gaps = 1/49 (2%) Frame = +2 Query: 449 VIAKIDDLTQKL-TVANADLAKQPIAHFVCQRNDCGSTRR*NGSARLRK 592 ++A +L Q++ VAN L P++H++ N G ++ + AR+RK Sbjct: 221 IMAPTRELCQQIYNVANK-LNNNPLSHWIVSCNVIGGEKKKSEKARIRK 268 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 8.3 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +2 Query: 101 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 226 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 874 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/43 (30%), Positives = 20/43 (46%) Frame = +3 Query: 486 RWPTQIWRSNRSLILFANEMIVARRDAETARQDCENARRKTAQ 614 RW T+IW L+ +M A R + D N+R+ +Q Sbjct: 677 RWLTKIWNCVHQLLEREKKMSDAMRQTKLTIVDDHNSRKLESQ 719 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 25.4 bits (53), Expect = 8.3 Identities = 14/43 (32%), Positives = 21/43 (48%) Frame = +3 Query: 183 STSTASTNRRLSMPTKSSTMLQIAGKAGRPAVFAPTHSAHYQI 311 S+S++ ++ R S T S + RP VF T +HY I Sbjct: 162 SSSSSKSSSRSSSRTTSHRTTSHKSSSYRPTVFPYTTISHYNI 204 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 8.3 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +3 Query: 528 LFANEMIVARRDAETARQDCE 590 +FANE+++ + DA++ +QD E Sbjct: 556 IFANEILIEQADAQSFKQDEE 576 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,966,925 Number of Sequences: 5004 Number of extensions: 62113 Number of successful extensions: 190 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 182 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 190 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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