Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120567.Seq
(799 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 194 6e-50
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 190 7e-49
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 190 9e-49
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 35 0.072
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 35 0.072
At1g11060.1 68414.m01267 expressed protein 30 1.5
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 29 3.6
At2g16365.2 68415.m06033 F-box family protein contains Pfam:PF00... 29 4.7
At2g16365.1 68415.m06032 F-box family protein contains Pfam:PF00... 29 4.7
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 6.2
At1g33110.1 68414.m04089 MATE efflux family protein similar to r... 28 6.2
At2g02610.1 68415.m00200 DC1 domain-containing protein contain... 28 8.3
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 194 bits (473), Expect = 6e-50
Identities = 96/171 (56%), Positives = 120/171 (70%), Gaps = 1/171 (0%)
Frame = +2
Query: 254 LDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPICLLW- 430
LDY ER+GY+KG+V +IIHDPGRGAPLA V FR P+++ +KELF+A E ++ L
Sbjct: 33 LDYGERNGYLKGLVTEIIHDPGRGAPLARVAFRHPFRYMKQKELFVAAEGMYTGQYLYCG 92
Query: 431 KESNS*SWKCDACWSYA*GYHCVQS*EKMGDRGRLARASGNFATVIGHNPDAKRTRVKLP 610
K++N S G +GDRG LARASG++A VI HNP++ TRVKLP
Sbjct: 93 KKANLMVGNVLPLGSIPEGAVICNVELHVGDRGALARASGDYAIVIAHNPESNTTRVKLP 152
Query: 611 SGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVQRNCWPYVRGV 763
SG+KK+LPS+ R M+G VAGGGR +KP LKAG AYHKYK +RNCWP VRGV
Sbjct: 153 SGSKKILPSACRAMIGQVAGGGRTEKPFLKAGNAYHKYKAKRNCWPVVRGV 203
Score = 87.0 bits (206), Expect = 1e-17
Identities = 54/121 (44%), Positives = 72/121 (59%), Gaps = 5/121 (4%)
Frame = +3
Query: 162 MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRS*ITLNVMDTSRELLRI*SMTLVEVHLWL 338
MGRVIRAQRKGA GSVF SHT RKG K RS L+ + + L + + + +
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRS---LDYGERNGYLKGLVTEIIHDPGRGA 57
Query: 339 -LYTSAIHTSSRQGRSSSLL---PKLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNL 506
L A R + L +YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+
Sbjct: 58 PLARVAFRHPFRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNV 117
Query: 507 E 509
E
Sbjct: 118 E 118
Score = 33.1 bits (72), Expect = 0.22
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = +1
Query: 763 AMNPVEHPHGGG 798
AMNPVEHPHGGG
Sbjct: 204 AMNPVEHPHGGG 215
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 190 bits (464), Expect = 7e-49
Identities = 95/171 (55%), Positives = 120/171 (70%), Gaps = 1/171 (0%)
Frame = +2
Query: 254 LDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPICLLW- 430
LD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A E ++ L
Sbjct: 32 LDFGERNGYLKGVVTEIIHDPGRGAPLARVTFRHPFRFKKQKELFVAAEGMYTGQFLYCG 91
Query: 431 KESNS*SWKCDACWSYA*GYHCVQS*EKMGDRGRLARASGNFATVIGHNPDAKRTRVKLP 610
K++ S G +GDRG LARASG++A VI HNPD+ TR+KLP
Sbjct: 92 KKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRGVLARASGDYAIVIAHNPDSDTTRIKLP 151
Query: 611 SGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVQRNCWPYVRGV 763
SG+KK++PS R M+G VAGGGR +KP+LKAG AYHKY+V+RN WP VRGV
Sbjct: 152 SGSKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKYRVKRNSWPKVRGV 202
Score = 90.2 bits (214), Expect = 1e-18
Identities = 55/120 (45%), Positives = 71/120 (59%), Gaps = 4/120 (3%)
Frame = +3
Query: 162 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRS*ITLNVMDTSRELLRI*SMTLVEVHLWL- 338
MGRVIRAQRKGAGSVF SHT RKG K RS L+ + + L + + + +
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRS---LDFGERNGYLKGVVTEIIHDPGRGAP 57
Query: 339 LYTSAIHTSSRQGRSSSLLPK---LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLE 509
L R + L +YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E
Sbjct: 58 LARVTFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVE 117
Score = 33.1 bits (72), Expect = 0.22
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = +1
Query: 763 AMNPVEHPHGGG 798
AMNPVEHPHGGG
Sbjct: 203 AMNPVEHPHGGG 214
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 190 bits (463), Expect = 9e-49
Identities = 94/171 (54%), Positives = 119/171 (69%), Gaps = 1/171 (0%)
Frame = +2
Query: 254 LDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPICLLW- 430
LD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A E ++ L
Sbjct: 32 LDFGERNGYLKGVVTEIIHDPGRGAPLARVAFRHPFRFKKQKELFVAAEGMYTGQFLYCG 91
Query: 431 KESNS*SWKCDACWSYA*GYHCVQS*EKMGDRGRLARASGNFATVIGHNPDAKRTRVKLP 610
K++ S G +GDRG ARASG++A VI HNPD +R+KLP
Sbjct: 92 KKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFARASGDYAIVIAHNPDNDTSRIKLP 151
Query: 611 SGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVQRNCWPYVRGV 763
SG+KK++PS R M+G VAGGGR +KP+LKAG AYHKY+V+RNCWP VRGV
Sbjct: 152 SGSKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKYRVKRNCWPKVRGV 202
Score = 91.5 bits (217), Expect = 6e-19
Identities = 55/120 (45%), Positives = 72/120 (60%), Gaps = 4/120 (3%)
Frame = +3
Query: 162 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRS*ITLNVMDTSRELLRI*SMTLVEVHLWL- 338
MGRVIRAQRKGAGSVF SHT RKG K RS L+ + + L + + + +
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRS---LDFGERNGYLKGVVTEIIHDPGRGAP 57
Query: 339 LYTSAIHTSSRQGRSSSLLPK---LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLE 509
L A R + L +YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E
Sbjct: 58 LARVAFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVE 117
Score = 33.1 bits (72), Expect = 0.22
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = +1
Query: 763 AMNPVEHPHGGG 798
AMNPVEHPHGGG
Sbjct: 203 AMNPVEHPHGGG 214
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 34.7 bits (76), Expect = 0.072
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +2
Query: 518 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPIL 697
G ++ RA+G A ++ P + +KLPSG K + + R +G V+ K +
Sbjct: 82 GQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTVSNPSHGTKKLY 140
Query: 698 KAGRA 712
KAG++
Sbjct: 141 KAGQS 145
Score = 29.9 bits (64), Expect = 2.0
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1
Query: 763 AMNPVEHPHGGG 798
AMNP +HPHGGG
Sbjct: 158 AMNPCDHPHGGG 169
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 34.7 bits (76), Expect = 0.072
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +2
Query: 518 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPIL 697
G ++ RA+G A ++ P + +KLPSG K + + R +G V+ K +
Sbjct: 82 GQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTVSNPSHGTKKLY 140
Query: 698 KAGRA 712
KAG++
Sbjct: 141 KAGQS 145
Score = 29.9 bits (64), Expect = 2.0
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1
Query: 763 AMNPVEHPHGGG 798
AMNP +HPHGGG
Sbjct: 158 AMNPCDHPHGGG 169
>At1g11060.1 68414.m01267 expressed protein
Length = 930
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = -3
Query: 509 LKIAHNGTLRHSSNRHHISNFKSCFLSTINKLACVEL 399
LKI N T + N++H+ FK C S ++++ EL
Sbjct: 422 LKIMENATFLSTDNQNHLLGFKKCLGSHDSRMSFTEL 458
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 766 MNPVEHPHGG 795
MNPVEHPHGG
Sbjct: 428 MNPVEHPHGG 437
>At2g16365.2 68415.m06033 F-box family protein contains Pfam:PF00646
F-box domain
Length = 455
Score = 28.7 bits (61), Expect = 4.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 574 VSNHSGEVSRGTCQTTSITHFLSR 503
V H GE S Q+ ++ HFLSR
Sbjct: 271 VDGHQGETSNSATQSMNVEHFLSR 294
>At2g16365.1 68415.m06032 F-box family protein contains Pfam:PF00646
F-box domain
Length = 778
Score = 28.7 bits (61), Expect = 4.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 574 VSNHSGEVSRGTCQTTSITHFLSR 503
V H GE S Q+ ++ HFLSR
Sbjct: 271 VDGHQGETSNSATQSMNVEHFLSR 294
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 6.2
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +1
Query: 667 WRWTY*QTYFESWKGIPQVQG 729
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At1g33110.1 68414.m04089 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 494
Score = 28.3 bits (60), Expect = 6.2
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = -3
Query: 620 WLQTVALLLYA*HQDCVQSQWRSFQRHVPDDLYHPFSLKIAHNGTL 483
WL +A LL+ C + WR F DL+ F L ++ G L
Sbjct: 227 WLPNIAQLLFVTCGGC-KDTWRGFSMMAFKDLWPVFKLSMSSGGML 271
>At2g02610.1 68415.m00200 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 27.9 bits (59), Expect = 8.3
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -3
Query: 539 VPDDLYHPFSLKIAHNGTLRHSSNRHHIS-NFKSCFLSTINKLACV 405
VP++L S + NG + H S+RHH+ + F+ ACV
Sbjct: 327 VPEELEDVESFRKIANGVILHFSHRHHLKFDISGVFVGNKFCQACV 372
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,613,454
Number of Sequences: 28952
Number of extensions: 404008
Number of successful extensions: 1054
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1051
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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