BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV120560.Seq (820 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 29 1.0 SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.4 SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosacch... 27 3.2 SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subuni... 27 4.2 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 26 5.6 SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 26 5.6 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 7.4 SPBC30B4.05 |kap109||karyopherin Kap109|Schizosaccharomyces pomb... 26 7.4 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 25 9.8 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 28.7 bits (61), Expect = 1.0 Identities = 30/112 (26%), Positives = 47/112 (41%), Gaps = 5/112 (4%) Frame = +2 Query: 5 EFSKISKLVALVVRKTFCNIFEINNRLVGVRVGANVQRRCGRRLTQIQHDRFASRHD--T 178 E+S+IS A +K I +NN + G G + R ++++ R D T Sbjct: 620 EYSQISGQDASNAQKRIEVIVNVNN-VYGTIFGNELAYR-QEKISKFTDPSSVIRSDLWT 677 Query: 179 ADAAFFSLQRQLFVVFVSSHRPFASSKSAHLTKL---LSSQATYLYHFACLM 325 + L R+ +S HR SK AH+ K+ L Q +L CL+ Sbjct: 678 VEVDHLDLLRKQIEDSISLHRDVRDSKDAHILKVSNKLRKQILHLVEQNCLL 729 >SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 773 Score = 27.5 bits (58), Expect = 2.4 Identities = 22/66 (33%), Positives = 31/66 (46%), Gaps = 4/66 (6%) Frame = +2 Query: 311 FACLMKYKDIQKYEVQQLIEWAINASPDMDLQQ--FRIEFMDKTT--ELNLRSCQPKSFT 478 F L + DI YE+ I+ AIN+S L+ R+ D TT E L + P+ F Sbjct: 567 FNFLERTSDISAYEIACAIKTAINSSKVKLLRSALARLSLFDSTTSREALLLAFVPEDFD 626 Query: 479 YTFTTI 496 F T+ Sbjct: 627 SLFKTL 632 >SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 683 Score = 27.1 bits (57), Expect = 3.2 Identities = 15/48 (31%), Positives = 24/48 (50%) Frame = +2 Query: 320 LMKYKDIQKYEVQQLIEWAINASPDMDLQQFRIEFMDKTTELNLRSCQ 463 LM K + VQ+++ +AIN S + DL+ + T N+R Q Sbjct: 495 LMNGKGNDEELVQKVLNYAINQSSNQDLRDRAFAYQRLLTPENVRKAQ 542 >SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subunit Orc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 707 Score = 26.6 bits (56), Expect = 4.2 Identities = 13/42 (30%), Positives = 18/42 (42%) Frame = +3 Query: 561 SRFCHAAVENDEGAILQRIFHTTVRHVPRSLYERQRFPHLPH 686 +RF HA+ +L V H + LY +P LPH Sbjct: 437 NRFTHASPNRSSCVVLMDELDQLVTHNQKVLYNFFNWPSLPH 478 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 26.2 bits (55), Expect = 5.6 Identities = 14/36 (38%), Positives = 17/36 (47%) Frame = +1 Query: 691 LIEIALDKEKYGTDITFVDSYQQEPPVRTLRLCRTI 798 LI I L KYGT F+ S R RLC ++ Sbjct: 204 LIPILLKVRKYGTSEKFILSVFHPDAARLYRLCSSL 239 >SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 26.2 bits (55), Expect = 5.6 Identities = 18/88 (20%), Positives = 38/88 (43%), Gaps = 1/88 (1%) Frame = +2 Query: 227 VSSHRPFASSKSAH-LTKLLSSQATYLYHFACLMKYKDIQKYEVQQLIEWAINASPDMDL 403 + + P + + H L + S+ T + AC Y D +K E + +I W + + Sbjct: 297 IKNLHPLTNKSTIHSLLSYVFSRQTQ--NIACEPMYIDYRKDETEAIIRWKTPLHAETCI 354 Query: 404 QQFRIEFMDKTTELNLRSCQPKSFTYTF 487 FR + + + ++R+ + K + F Sbjct: 355 NAFRTQERKQNSHDDIRAHRKKGSSRPF 382 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.8 bits (54), Expect = 7.4 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = -1 Query: 94 DADEPIIYFENITECLTDDQCDKFTYFAELK 2 DAD I+ NI LT ++CD+F Y +K Sbjct: 1023 DADYAIL---NILNLLTSNECDEFDYSVLMK 1050 >SPBC30B4.05 |kap109||karyopherin Kap109|Schizosaccharomyces pombe|chr 2|||Manual Length = 967 Score = 25.8 bits (54), Expect = 7.4 Identities = 12/29 (41%), Positives = 21/29 (72%) Frame = -2 Query: 690 LNVVNEETFDVHIMIAAHGAL*YEKYVVK 604 LN+V E+T D++I +AA +L ++ Y+ K Sbjct: 39 LNIVAEDTVDINIKLAA--SLYFKNYIKK 65 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 25.4 bits (53), Expect = 9.8 Identities = 17/79 (21%), Positives = 38/79 (48%), Gaps = 1/79 (1%) Frame = +1 Query: 523 IIDDMVYTRD-KSSLDFVMQQLKTMKVLFYNVFFILQCAMCRDHYMNVKGFLIYHIELIE 699 +ID VY+ +S + V Q ++++ + ++ L CR Y N+ + I+ Sbjct: 285 VIDLFVYSGSTESPKETVKQFVQSIGKPAMHQYWTLGYHSCRWGYTNITEIMDVRQNYID 344 Query: 700 IALDKEKYGTDITFVDSYQ 756 + E + +DI +++ Y+ Sbjct: 345 ADIPVETFWSDIDYMEKYR 363 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,396,333 Number of Sequences: 5004 Number of extensions: 70751 Number of successful extensions: 171 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 400438000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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